Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I184 R1	278	29.6	1166432	97.3%	1134938	124.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	527,133	A→G	V182A (GTT→GCT)	allS ←	HTH‑type transcriptional activator AllS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	527,133	0	A	G	100.0%	130.4 / NA	36	V182A (GTT→GCT)	allS	HTH‑type transcriptional activator AllS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

TCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAA  >  NZ_CP009273/527007‑527263

tCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGc                                                                                                                         <  1:370164/138‑1 (MQ=255)

                  ttttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGa                                                                                                      >  2:200385/1‑139 (MQ=255)

                     tGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACa                                                                                                    <  1:101304/138‑1 (MQ=255)

                       cccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAg                                                                                                 <  1:355823/139‑1 (MQ=255)

                           ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcgc                                                                                              >  2:339006/1‑138 (MQ=255)

                           ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcgc                                                                                              >  1:560154/1‑138 (MQ=255)

                             cAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCAt                                                                                           <  2:235525/139‑1 (MQ=255)

                                  gCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAc                                                                                      <  2:78462/139‑1 (MQ=255)

                                  gCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAc                                                                                      <  1:339006/139‑1 (MQ=255)

                                   ccAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc                                                                                     <  1:147460/139‑1 (MQ=255)

                                   ccAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc                                                                                     <  2:560154/139‑1 (MQ=255)

                                      ggCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGAt                                                                                  <  2:260978/139‑1 (MQ=255)

                                      ggCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGAt                                                                                  >  1:505249/1‑139 (MQ=255)

                                            gcgTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAg                                                                            >  1:284350/1‑139 (MQ=255)

                                                            cGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAgg                                                            >  1:144775/1‑139 (MQ=255)

                                                             gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTaa                                                               >  1:401761/1‑135 (MQ=255)

                                                             gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTaa                                                               <  2:401761/135‑1 (MQ=255)

                                                              ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCa                                                                                            <  1:483135/105‑1 (MQ=255)

                                                              ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCa                                                                                            >  2:483135/1‑105 (MQ=255)

                                                              ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa                                                                             >  2:296125/1‑120 (MQ=255)

                                                              ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa                                                                             <  1:296125/120‑1 (MQ=255)

                                                                  cTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTg                                                      >  2:157688/1‑139 (MQ=255)

                                                                         cAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAg                                                  <  2:447075/136‑1 (MQ=255)

                                                                          aaTATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               <  1:421790/138‑1 (MQ=255)

                                                                          aaTATTGACCGCCGGAAAGCGCCACAACTGCGCTTCTGTTAGCGGCTTTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAACGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               <  2:392345/138‑1 (MQ=255)

                                                                               tGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACa                                                                                                    >  1:289822/1‑80 (MQ=255)

                                                                               tGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACa                                                                                                    <  2:289822/80‑1 (MQ=255)

                                                                                    gccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCg                                    >  1:195037/1‑139 (MQ=255)

                                                                                                                ttAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCt          <  1:157688/137‑1 (MQ=255)

                                                                                                                ttAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCt          <  2:424796/137‑1 (MQ=255)

                                                                                                                ttAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTc        >  1:424796/1‑139 (MQ=255)

                                                                                                                       ctctTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               <  2:274708/93‑1 (MQ=255)

                                                                                                                       ctctTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               >  1:274708/1‑93 (MQ=255)

                                                                                                                        tctTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACaa  >  2:6868/1‑137 (MQ=255)

                                                                                                                          ttCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAg                    >  2:219531/1‑117 (MQ=255)

                                                                                                                          ttCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAg                    <  1:219531/117‑1 (MQ=255)

TCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAA  >  NZ_CP009273/527007‑527263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGAC  >  NZ_CP009273/527006‑527278

ATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGC                                                                                                                             <  SRR3722139.374612/150‑1 (MQ=60)

                 CTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCC                                                                                                            >  SRR3722139.566562/1‑150 (MQ=60)

                     TTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTG                                                                                                        <  SRR3722139.102502/150‑1 (MQ=60)

                       GCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAC                                                                                                     <  SRR3722139.360125/151‑1 (MQ=60)

                            GGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATC                                                                                                >  SRR3722139.511075/1‑151 (MQ=60)

                              cggagatgtgtataagagacaGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATC                                                                                                <  SRR3722139.488761/128‑1 (MQ=60)

                                  agataattcttttttttttcaagcagaagacggcatacgagatctagtacggtctcgggggctcggagatgtgtataagagacaGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAG                                                                                           <  SRR3722139.438398/66‑1 (MQ=60)

                                  CGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGG                                                                                          <  SRR3722139.343133/151‑1 (MQ=60)

                                  CGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGG                                                                                          >  SRR3722139.287927/1‑151 (MQ=60)

                                   GCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGG                                                                                         <  SRR3722139.149257/151‑1 (MQ=60)

                                           aagagacaGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGAC                                                                                 <  SRR3722139.299836/143‑1 (MQ=60)

                                                  TGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGT                                                                          >  SRR3722139.146516/1‑151 (MQ=60)

                                                   GGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGT                                                                          >  SRR3722139.406523/1‑150 (MQ=60)

                                                                     GTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCtgtctcttatacacatctccgagcccacgagaccgtactagatctcgt                                                       >  SRR3722139.293473/1‑103 (MQ=60)

                                                                          CAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGT                                                  >  SRR3722139.197440/1‑151 (MQ=60)

                                                                          CAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCG                                                   <  SRR3722139.426763/150‑1 (MQ=60)

                                                                                                     ataagagacagGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTT                        <  SRR3722139.222217/139‑1 (MQ=60)

                                                                                                      CTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCG                      >  SRR3722139.429800/1‑151 (MQ=60)

                                                                                                             TCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGctgtctcttatacacatctccgagcccacgagaccg               >  SRR3722139.278189/1‑115 (MQ=60)

                                                                                                                GTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAG              <  SRR3722139.159612/149‑1 (MQ=60)

                                                                                                                          CTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGAC  >  SRR3722139.40034/1‑151 (MQ=60)

ATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAATAGCGAGTCCCAGAC  >  NZ_CP009273/527006‑527278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`