Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I177 R1
|
141 |
27.5 |
996908 |
97.7% |
973979 |
124.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
478,146 |
A→C |
F572V (TTT→GTT) |
acrB ← |
efflux RND transporter permease AcrB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 478,146 | 0 | A | C | 100.0%
| 86.9
/ NA
| 24 | F572V (TTT→GTT) | acrB | efflux RND transporter permease AcrB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (13/11); total (13/11) |
AAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCT > NZ_CP009273/478016‑478272
|
aagccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCt > 2:16723/1‑139 (MQ=255)
cGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTgg > 2:210896/1‑139 (MQ=255)
aCTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCaagaag > 1:482536/1‑137 (MQ=255)
cGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTgg > 2:373158/1‑139 (MQ=255)
tctttGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACg < 1:273020/138‑1 (MQ=255)
gTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGAt < 1:63930/139‑1 (MQ=255)
tACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCAc > 1:316223/1‑138 (MQ=255)
aTTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc < 1:373158/139‑1 (MQ=255)
gCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATa < 2:482536/139‑1 (MQ=255)
cACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGACCATGCCGACCACGATGATCAGATa > 2:206117/1‑138 (MQ=255)
gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCa < 1:447619/135‑1 (MQ=255)
gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCa > 2:447619/1‑135 (MQ=255)
gtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAg > 1:267662/1‑78 (MQ=255)
gtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAg < 2:267662/78‑1 (MQ=255)
tACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGt > 2:138384/1‑138 (MQ=255)
tACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa < 2:35207/139‑1 (MQ=255)
tACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa < 1:16723/139‑1 (MQ=255)
cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa > 2:236316/1‑137 (MQ=255)
cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa < 1:236316/137‑1 (MQ=255)
ccTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGccc > 2:293843/1‑139 (MQ=255)
ggCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATGCAGCACCAGGTAACGCCCCGTa < 1:237063/126‑1 (MQ=255)
ggCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTa > 2:237063/1‑126 (MQ=255)
cAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCg > 2:171342/1‑139 (MQ=255)
ccATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCt < 1:450255/139‑1 (MQ=255)
|
AAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCT > NZ_CP009273/478016‑478272
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCG > NZ_CP009273/478027‑478289
|
GGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGG > SRR3722131.487711/1‑149 (MQ=60)
GACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTgtctcttatacacatctccgagcccac > SRR3722131.315193/1‑124 (MQ=60)
TTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGC < SRR3722131.276191/150‑1 (MQ=60)
GGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCG < SRR3722131.64630/151‑1 (MQ=60)
TAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACG > SRR3722131.319812/1‑150 (MQ=60)
GTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtcttctgcttg > SRR3722131.95282/1‑87 (MQ=60)
CATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCAC < SRR3722131.377289/151‑1 (MQ=60)
GCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCtgtctcttatacacatctccgagcccacgagaccgtactagatctcgtat > SRR3722131.270782/1‑101 (MQ=60)
TTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGC < SRR3722131.452442/150‑1 (MQ=60)
GTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTAC < SRR3722131.16872/151‑1 (MQ=60)
GTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTAC < SRR3722131.239116/151‑1 (MQ=60)
caGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATGCAGCACCAGGTAACGCCCCGTACTGCGCAGAAT < SRR3722131.239874/149‑1 (MQ=60)
gggctcggagatgtgtataagagacaGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATAC < SRR3722131.494034/125‑1 (MQ=60)
ACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGT < SRR3722131.455101/151‑1 (MQ=60)
CAGGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGG < SRR3722131.88321/151‑1 (MQ=60)
GTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCG > SRR3722131.355405/1‑151 (MQ=60)
|
GGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCGGTGTAGTGGTGCG > NZ_CP009273/478027‑478289
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
