Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 151,620 C→A 15.9% K98N (AAG→AAT)  htrE ← putative outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273151,6200CA15.9% 677.6 / 74.7 212K98N (AAG→AAT) htrEputative outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base C (98/80);  new base A (18/16);  total (116/96)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

AGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATC  >  CP009273/151471‑151769
                                                                                                                                                     |                                                                                                                                                     
aGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTc                                                                                                                                                       <  1:2508028/150‑1 (MQ=255)
aGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTc                                                                                                                                                       <  1:2251137/150‑1 (MQ=255)
 gACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCt                                                                                                                                                      <  2:3958256/150‑1 (MQ=255)
  aCGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCtt                                                                                                                                                     <  1:1648754/150‑1 (MQ=255)
  aCGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCtt                                                                                                                                                     <  2:2393194/150‑1 (MQ=255)
     ttGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTaa                                                                                                                                                  >  2:4352340/1‑150 (MQ=255)
          cGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGa                                                                                                                                             <  1:2492480/150‑1 (MQ=255)
          cGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGa                                                                                                                                             <  1:2491453/150‑1 (MQ=255)
          cGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGa                                                                                                                                             <  1:1603893/150‑1 (MQ=255)
             tAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATAc                                                                                                                                          <  2:829818/150‑1 (MQ=255)
             tAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATAc                                                                                                                                          >  1:2980081/1‑150 (MQ=255)
                 gTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGc                                                                                                                                      <  1:3349530/150‑1 (MQ=255)
                  tCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTAGATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGcc                                                                                                                                     >  2:3839280/1‑150 (MQ=255)
                   cATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCt                                                                                                                                    <  2:1658065/150‑1 (MQ=255)
                    aTAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTg                                                                                                                                   >  2:1095681/1‑150 (MQ=255)
                     tAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTgg                                                                                                                                  >  1:2421363/1‑150 (MQ=255)
                      aaCGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTggg                                                                                                                                 >  2:3459693/1‑150 (MQ=255)
                         gAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGt                                                                                                                              <  1:2165647/150‑1 (MQ=255)
                         gAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGt                                                                                                                              >  1:3798338/1‑150 (MQ=255)
                            cAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCt                                                                                                                           <  1:2744473/150‑1 (MQ=255)
                            cAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCt                                                                                                                           <  1:429924/150‑1 (MQ=255)
                            cAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCt                                                                                                                           <  2:3597346/150‑1 (MQ=255)
                            cAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCt                                                                                                                           <  2:3249808/150‑1 (MQ=255)
                             aGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCtt                                                                                                                          >  2:452229/1‑150 (MQ=255)
                              gAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCttt                                                                                                                         <  1:4442382/150‑1 (MQ=255)
                                aGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCttttt                                                                                                                       <  1:2729112/150‑1 (MQ=255)
                                  ccTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTc                                                                                                                     >  2:4177849/1‑150 (MQ=255)
                                   cTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTcc                                                                                                                    >  1:4240827/1‑150 (MQ=255)
                                     gAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCtt                                                                                                                  <  1:1171953/150‑1 (MQ=255)
                                     gAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCtt                                                                                                                  <  2:1729976/150‑1 (MQ=255)
                                     gAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCtt                                                                                                                  >  2:2737543/1‑150 (MQ=255)
                                       gggATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCa                                                                                                                <  2:119281/150‑1 (MQ=255)
                                          aTAATTTCCGTCATATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTCATATGAAACGGCAATAAAGTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAAtt                                                                                                             <  2:4620833/150‑1 (MQ=255)
                                           tAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTg                                                                                                            <  1:4603547/150‑1 (MQ=255)
                                           tAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTg                                                                                                            <  1:3787911/150‑1 (MQ=255)
                                                tCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCGTCAATTGCGACa                                                                                                       >  2:3155857/1‑150 (MQ=255)
                                                   gTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAt                                                                                                    <  1:4047146/150‑1 (MQ=255)
                                                     cAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGt                                                                                                  >  2:4467807/1‑150 (MQ=255)
                                                       aaTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTaa                                                                                                <  2:1387246/150‑1 (MQ=255)
                                                        aTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAATTTCTTTAATGTGATACAAGCCTGGGCGTTATTTTTTCCTTACATTGCGAAAAAAGTact                                                                                               >  1:3887230/1‑148 (MQ=255)
                                                        aTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAAt                                                                                               <  1:3501387/150‑1 (MQ=255)
                                                              gCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGTTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTg                                                                                         <  2:2474135/150‑1 (MQ=255)
                                                                aaTTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTATTGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGt                                                                                       >  2:2304084/1‑150 (MQ=255)
                                                                aaTTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGt                                                                                       >  2:3684649/1‑150 (MQ=255)
                                                                aaTTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGt                                                                                       <  1:549421/150‑1 (MQ=255)
                                                                  ttGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTa                                                                                     <  1:455510/150‑1 (MQ=255)
                                                                   tGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTaa                                                                                    <  1:2387200/150‑1 (MQ=255)
                                                                     ccGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATAGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAAATGGGACAAATTTAAAAAAATGGTTAATg                                                                                  >  1:2361047/1‑150 (MQ=255)
                                                                      cGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTACTTCAATTGCGACAAATGAAATACTTTGGTTAATGa                                                                                 >  2:640819/1‑150 (MQ=255)
                                                                        aGCGTTTCATCCCTGGCATGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAtt                                                                               <  1:4141011/150‑1 (MQ=255)
                                                                         gCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttg                                                                              <  1:4269326/150‑1 (MQ=255)
                                                                           gTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCATGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATTAttggt                                                                            >  2:2624808/1‑150 (MQ=255)
                                                                             ttCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggttg                                                                          <  1:1189054/150‑1 (MQ=255)
                                                                             ttCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggttg                                                                          <  2:870468/150‑1 (MQ=255)
                                                                                aTCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCTGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTc                                                                       <  1:318240/150‑1 (MQ=255)
                                                                                aTCCCTGGCAAACAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTc                                                                       <  2:2447877/150‑1 (MQ=255)
                                                                                 tCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCg                                                                      >  1:3230256/1‑150 (MQ=255)
                                                                                 tCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCg                                                                      <  1:1894320/150‑1 (MQ=255)
                                                                                   ccTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGtt                                                                    >  2:517498/1‑150 (MQ=255)
                                                                                    cTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGttt                                                                   <  2:636641/150‑1 (MQ=255)
                                                                                     tGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTa                                                                  <  2:694963/150‑1 (MQ=255)
                                                                                        cAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTACTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACAt                                                               >  1:2805561/1‑150 (MQ=255)
                                                                                          aGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATaa                                                             >  2:3344512/1‑150 (MQ=255)
                                                                                          aGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATaa                                                             <  2:1746173/150‑1 (MQ=255)
                                                                                            cAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAac                                                           >  1:2626669/1‑150 (MQ=255)
                                                                                             aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAaca                                                          >  1:4204365/1‑150 (MQ=255)
                                                                                             aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGAAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAaca                                                          >  1:2568702/1‑150 (MQ=255)
                                                                                              gAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATTTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAacac                                                         >  1:2862329/1‑150 (MQ=255)
                                                                                               aaCGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACt                                                        >  2:4346530/1‑150 (MQ=255)
                                                                                                  ggCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAc                                                     <  1:4352354/150‑1 (MQ=255)
                                                                                                   gCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcg                                                    >  2:1040075/1‑150 (MQ=255)
                                                                                                    cTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTATTTTTTCCTTCAATTGCGACAAATGTAATACTTTTGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgt                                                   >  1:3946164/1‑150 (MQ=255)
                                                                                                       tttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgtcgt                                                >  2:2731439/1‑150 (MQ=255)
                                                                                                          cGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTaaa                                             >  2:2216778/1‑150 (MQ=255)
                                                                                                           gTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAac                                            >  2:259498/1‑150 (MQ=255)
                                                                                                           gTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAac                                            >  1:2307475/1‑150 (MQ=255)
                                                                                                            ttatttatATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAaca                                           >  1:1029856/1‑150 (MQ=255)
                                                                                                             tatttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAacac                                          >  2:1514196/1‑150 (MQ=255)
                                                                                                                  atatCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGt                                     <  2:4256071/150‑1 (MQ=255)
                                                                                                                  atatCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGt                                     >  1:1986042/1‑150 (MQ=255)
                                                                                                                   tatCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGta                                    >  2:3731393/1‑150 (MQ=255)
                                                                                                                     tCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGtata                                  <  1:3437644/150‑1 (MQ=255)
                                                                                                                      cGGGAGAATTAATATGAAACTGCAATAAAGTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAg                                 <  2:4388462/150‑1 (MQ=255)
                                                                                                                        ggAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCg                               <  1:2020041/150‑1 (MQ=255)
                                                                                                                          agaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCggg                             <  2:546205/150‑1 (MQ=255)
                                                                                                                           gaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgggg                            <  1:1608152/150‑1 (MQ=255)
                                                                                                                               tAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTAc                        >  1:2777519/1‑150 (MQ=255)
                                                                                                                               tAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAAAACTGACGTCGTAAACACCCGGTATAGCGGGGGTAc                        >  1:2833232/1‑150 (MQ=255)
                                                                                                                                aaTATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTAcc                       <  1:3956995/150‑1 (MQ=255)
                                                                                                                                  tatGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCtt                     <  2:3768837/150‑1 (MQ=255)
                                                                                                                                    tGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCa                   >  2:3071541/1‑150 (MQ=255)
                                                                                                                                      aaaCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATTATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACt                 >  1:4441838/1‑150 (MQ=255)
                                                                                                                                      aaaCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCaat                 <  2:2532289/150‑3 (MQ=255)
                                                                                                                                      aaaCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACt                 >  1:3071750/1‑150 (MQ=255)
                                                                                                                                        aCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGt               >  2:2756230/1‑150 (MQ=255)
                                                                                                                                         cTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTa              <  2:1825666/150‑1 (MQ=255)
                                                                                                                                           gCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTAcc            >  1:1516973/1‑150 (MQ=255)
                                                                                                                                              aTAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTACTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACAACCGGTATAGCGGGGTTACCTTCACTGTACCGGc         >  1:886904/1‑150 (MQ=255)
                                                                                                                                               tAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCt        >  2:1619492/1‑150 (MQ=255)
                                                                                                                                                   ttCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgaga    >  2:3292214/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATC  >  CP009273/151471‑151769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A