Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 151,620 C→A 36.7% K98N (AAG→AAT)  htrE ← putative outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273151,6200CA36.7% 313.9 / 222.1 218K98N (AAG→AAT) htrEputative outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base C (67/71);  new base A (41/39);  total (108/110)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 219 total aligned reads displayed.

CGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGAT  >  CP009273/151474‑151768
                                                                                                                                                  |                                                                                                                                                    
cGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCttt                                                                                                                                                   >  2:1130038/1‑150 (MQ=255)
 gTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTa                                                                                                                                                  <  2:1377320/150‑1 (MQ=255)
       cGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGa                                                                                                                                            <  1:1232579/150‑1 (MQ=255)
       cGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAAGATGAAACTGCAATAAATTCTTTAATGTGa                                                                                                                                            >  2:3867037/1‑150 (MQ=255)
        gTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGAt                                                                                                                                           <  2:4145726/150‑1 (MQ=255)
         ttAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATa                                                                                                                                          >  2:772997/1‑150 (MQ=255)
         ttAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATa                                                                                                                                          <  2:3076631/150‑1 (MQ=255)
          tAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATAc                                                                                                                                         <  1:3540307/150‑1 (MQ=255)
           aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACa                                                                                                                                        <  2:2325609/150‑1 (MQ=255)
           aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACa                                                                                                                                        >  1:3958042/1‑150 (MQ=255)
            aCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACaa                                                                                                                                       <  1:1772746/150‑1 (MQ=255)
              gTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGc                                                                                                                                     >  1:514116/1‑150 (MQ=255)
              gTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGc                                                                                                                                     >  1:2448479/1‑150 (MQ=255)
                cATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCt                                                                                                                                   >  2:3123900/1‑150 (MQ=255)
                  tAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTgg                                                                                                                                 <  1:3150888/150‑1 (MQ=255)
                  tAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTgg                                                                                                                                 <  2:3512248/150‑1 (MQ=255)
                   aaCGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTggg                                                                                                                                <  1:709816/150‑1 (MQ=255)
                       aaCAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGtt                                                                                                                            >  2:2426321/1‑150 (MQ=255)
                       aaCAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGtt                                                                                                                            >  2:2426758/1‑150 (MQ=255)
                        aCAGACGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGCACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTc                                                                                                                           <  1:3333196/150‑1 (MQ=255)
                        aCAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTc                                                                                                                           <  1:3194099/150‑1 (MQ=255)
                         cAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCt                                                                                                                          <  1:3063084/150‑1 (MQ=255)
                          aGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCtt                                                                                                                         <  2:2078377/150‑1 (MQ=255)
                          aGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCtt                                                                                                                         <  1:931289/150‑1 (MQ=255)
                            aaGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCtttt                                                                                                                       <  1:2244335/150‑1 (MQ=255)
                                  gAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCtt                                                                                                                 <  2:3713345/150‑1 (MQ=255)
                                  gAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCtt                                                                                                                 <  1:3172125/150‑1 (MQ=255)
                                  gAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCtt                                                                                                                 <  1:1427054/150‑1 (MQ=255)
                                   aGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTc                                                                                                                <  2:2007590/150‑1 (MQ=255)
                                   aGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTc                                                                                                                <  2:1320220/150‑1 (MQ=255)
                                     ggATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCaa                                                                                                              >  1:273730/1‑150 (MQ=255)
                                     ggATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCaa                                                                                                              <  2:2727361/150‑1 (MQ=255)
                                        tAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTg                                                                                                           <  2:3294618/150‑1 (MQ=255)
                                          aTTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCg                                                                                                         <  1:1037746/150‑1 (MQ=255)
                                          aTTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCg                                                                                                         >  2:1511988/1‑150 (MQ=255)
                                            ttCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGAc                                                                                                       >  2:2055402/1‑150 (MQ=255)
                                                 tCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATg                                                                                                  <  1:4020105/150‑1 (MQ=255)
                                                 tCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATg                                                                                                  <  2:2372551/150‑1 (MQ=255)
                                                  cAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGt                                                                                                 <  1:3519748/150‑1 (MQ=255)
                                                   aaaTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTa                                                                                                >  2:345860/1‑150 (MQ=255)
                                                    aaTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCATAAATAGCGACaaaattaa                                                                                               >  1:240291/1‑145 (MQ=255)
                                                     aTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAAt                                                                                              <  1:1904307/150‑1 (MQ=255)
                                                        gAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACt                                                                                           >  2:2533641/1‑150 (MQ=255)
                                                            cAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTgg                                                                                       >  2:449893/1‑150 (MQ=255)
                                                               ttGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCATTTTTCCTTCAAATGCGACAAATGTAATACATTGGTTa                                                                                    >  1:1541872/1‑150 (MQ=255)
                                                                tGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTaa                                                                                   >  2:1656643/1‑150 (MQ=255)
                                                                 gCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAt                                                                                  <  2:3025470/150‑1 (MQ=255)
                                                                 gCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAt                                                                                  <  1:1500503/150‑1 (MQ=255)
                                                                    gAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAt                                                                               >  2:2549610/1‑150 (MQ=255)
                                                                      gCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttg                                                                             <  1:679246/150‑1 (MQ=255)
                                                                      gCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttg                                                                             <  1:824868/150‑1 (MQ=255)
                                                                      gCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttg                                                                             <  1:1092814/150‑1 (MQ=255)
                                                                        gTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggt                                                                           <  1:1985021/150‑1 (MQ=255)
                                                                         tttCATCCCTGGCAAGCAGAACGGCTTTTTCGTTTTTTATATAGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggtt                                                                          <  2:1255716/150‑1 (MQ=255)
                                                                         tttCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggtt                                                                          <  1:2839646/150‑1 (MQ=255)
                                                                          ttCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggttg                                                                         <  1:3628439/150‑1 (MQ=255)
                                                                             aTCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTc                                                                      <  1:1817932/150‑1 (MQ=255)
                                                                                 cTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGttt                                                                  <  2:121265/150‑1 (MQ=255)
                                                                                  tGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTa                                                                 >  2:2045489/1‑150 (MQ=255)
                                                                                    gCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACa                                                               <  2:2170500/150‑1 (MQ=255)
                                                                                     cAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTTGTTGGTCGTTTACAt                                                              >  2:3498700/1‑150 (MQ=255)
                                                                                       aGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATaa                                                            >  2:1858474/1‑150 (MQ=255)
                                                                                         cAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAac                                                          <  1:2951466/150‑1 (MQ=255)
                                                                                         cAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAac                                                          <  1:1475023/150‑1 (MQ=255)
                                                                                          aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAaca                                                         >  2:1503244/1‑150 (MQ=255)
                                                                                          aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTAAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAaca                                                         >  2:33836/1‑150 (MQ=255)
                                                                                           gAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTAAATTGCGACAAAAGTAATAATTTGGTTAATGATTGGTTGGTCGTTTACATAAacac                                                        >  1:796544/1‑150 (MQ=255)
                                                                                               ggCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAc                                                    <  2:2875194/150‑1 (MQ=255)
                                                                                                 cTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgt                                                  <  2:1542318/150‑1 (MQ=255)
                                                                                                  tttttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTGGGTCGTTTACATAAACACTGAcgtc                                                 <  2:807323/150‑1 (MQ=255)
                                                                                                    tttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTTGTAGTTTACATAAACACTGAcgtcgt                                               >  2:3681168/1‑150 (MQ=255)
                                                                                                    tttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGAGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgtcgt                                               >  2:2764609/1‑150 (MQ=255)
                                                                                                    tttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATAATTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgtcgt                                               >  1:487940/1‑150 (MQ=255)
                                                                                                     ttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTa                                              >  1:2137594/1‑150 (MQ=255)
                                                                                                       cGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTaaa                                            >  2:2165204/1‑150 (MQ=255)
                                                                                                       cGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTaaa                                            >  1:3478227/1‑150 (MQ=255)
                                                                                                          tatttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTAAATTGCGACAAATGTAATACTTTGGTTAATGATTTGTTGGTCGTTTACATAAACACTGACGTCGTaaaaaa                                         >  1:69587/1‑147 (MQ=255)
                                                                                                           atttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACAcc                                        >  2:4142673/1‑150 (MQ=255)
                                                                                                             ttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTACTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGGTTACATAAACACTGACGTCGTAAACACCCg                                      >  1:2919171/1‑150 (MQ=255)
                                                                                                                tatCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGta                                   <  2:2598762/150‑1 (MQ=255)
                                                                                                                    gggAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGGATAGc                               >  1:74312/1‑150 (MQ=255)
                                                                                                                      gagaATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACGTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgg                             >  1:3273118/1‑150 (MQ=255)
                                                                                                                       agaATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCggg                            >  2:828002/1‑150 (MQ=255)
                                                                                                                        gaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgggg                           <  1:934705/150‑1 (MQ=255)
                                                                                                                        gaATTAATATGAAACTGCAATAAATTATTTAACGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgggg                           >  2:3952975/1‑150 (MQ=255)
                                                                                                                                atGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTc                   <  1:3345674/150‑1 (MQ=255)
                                                                                                                                atGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTc                   <  1:377071/150‑1 (MQ=255)
                                                                                                                                  gAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCAc                 <  2:579472/150‑1 (MQ=255)
                                                                                                                                  gAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATAATTTGGTTAATTATTGGTTGGTCGTTTACATAAAAACAGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCAc                 >  1:3657504/1‑150 (MQ=255)
                                                                                                                                     aCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAAGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGt              >  1:3889427/1‑150 (MQ=255)
                                                                                                                                      cTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTa             <  1:2689269/150‑1 (MQ=255)
                                                                                                                                        gCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTAcc           >  2:3186029/1‑150 (MQ=255)
                                                                                                                                        gCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTAcc           >  2:3185414/1‑150 (MQ=255)
                                                                                                                                          aaTAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTAAATTGCGACAAATGTAATACATTGGTTAATGATTGGTTGGTCGTTTAAATAAACACTGAAGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCgg         >  1:425796/1‑150 (MQ=255)
                                                                                                                                           aTAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATAATTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGc        >  2:2941157/1‑150 (MQ=255)
                                                                                                                                           aTAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCATAAATTGAGACAAAATTAATAAGTTGGTTAATGTTTGGTTTGTCGTTTAAATAAAAACAGACGTCGTAAAAAACCCGTATAGCGGGGTTACCTTAACGGTAACGGc        >  1:1093972/1‑150 (MQ=255)
                                                                                                                                            tAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCt       <  2:3664993/150‑1 (MQ=255)
                                                                                                                                            tAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCt       <  2:3665038/150‑1 (MQ=255)
                                                                                                                                               aTTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgag    <  2:733339/150‑1 (MQ=255)
                                                                                                                                                 tCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGAt  >  2:1456742/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGAT  >  CP009273/151474‑151768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A