Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 151,620 C→A 54.2% K98N (AAG→AAT)  htrE ← putative outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273151,6200CA54.2% 15.0 / inf 212K98N (AAG→AAT) htrEputative outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base C (44/53);  new base A (78/37);  total (122/90)
Fisher's exact test for biased strand distribution p-value = 1.30e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

AGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGA  >  CP009273/151471‑151767
                                                                                                                                                     |                                                                                                                                                   
aGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATAAATATGAAACTGCAATAAATTc                                                                                                                                                     >  2:2899747/1‑150 (MQ=255)
  aCGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCtt                                                                                                                                                   <  2:3181659/150‑1 (MQ=255)
   cGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCttt                                                                                                                                                  <  1:3017379/150‑1 (MQ=255)
   cGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTAttt                                                                                                                                                  >  2:2647066/1‑150 (MQ=255)
    gTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTa                                                                                                                                                 <  1:3232164/150‑1 (MQ=255)
    gTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTa                                                                                                                                                 >  2:3723663/1‑150 (MQ=255)
       gATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAAtg                                                                                                                                              <  2:1798060/150‑1 (MQ=255)
           gTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGAt                                                                                                                                          >  1:4538469/1‑150 (MQ=255)
             tAACTTCATAACGAACAGAAGCCTGAGGGATAATTTCCTTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCTTTATTTATATCGGGATAATTAATATGAAACTGCAATAAATTCTTTAATGTGATAc                                                                                                                                        <  2:2440534/150‑1 (MQ=255)
                 gTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGc                                                                                                                                    >  2:4536961/1‑150 (MQ=255)
                 gTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGc                                                                                                                                    >  2:3306701/1‑150 (MQ=255)
                 gTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGc                                                                                                                                    >  2:4001066/1‑150 (MQ=255)
                  tCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGcc                                                                                                                                   >  2:1367215/1‑150 (MQ=255)
                  tCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGcc                                                                                                                                   >  2:3814297/1‑150 (MQ=255)
                    aTAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTg                                                                                                                                 >  2:244574/1‑150 (MQ=255)
                     tAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTgg                                                                                                                                <  2:2743034/150‑1 (MQ=255)
                         gAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGt                                                                                                                            >  1:3665806/1‑150 (MQ=255)
                           aCAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTc                                                                                                                          <  1:4347207/150‑1 (MQ=255)
                           aCAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTc                                                                                                                          >  1:1479482/1‑150 (MQ=255)
                             aGAATCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCtt                                                                                                                        >  2:1536329/1‑150 (MQ=255)
                             aGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCtt                                                                                                                        >  2:2992254/1‑150 (MQ=255)
                              gAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCttt                                                                                                                       <  2:2580424/150‑1 (MQ=255)
                               aaGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCtttt                                                                                                                      <  1:2674224/150‑1 (MQ=255)
                                aGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCttttt                                                                                                                     <  1:3390692/150‑1 (MQ=255)
                                aGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCttttt                                                                                                                     >  2:3678468/1‑150 (MQ=255)
                                  ccTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTc                                                                                                                   >  1:226135/1‑150 (MQ=255)
                                   cTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTcc                                                                                                                  <  2:3722953/150‑1 (MQ=255)
                                    tGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCt                                                                                                                 <  1:2794392/150‑1 (MQ=255)
                                          aTAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAAtt                                                                                                           >  2:195100/1‑150 (MQ=255)
                                           tAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTg                                                                                                          <  1:1186016/150‑1 (MQ=255)
                                           tAATTTCCGTCAAATTGAGGCAATTGACGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTATTTATTTATATCGGGAGAATTAATATTAAACTGAAATAAATTATTTAATGTGATACAATAATGTGAGTAATTTTTTCCTTCAATTg                                                                                                          >  2:1359928/1‑150 (MQ=255)
                                             aTTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCg                                                                                                        <  2:3269428/150‑1 (MQ=255)
                                             aTTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCg                                                                                                        <  1:2048008/150‑1 (MQ=255)
                                               ttCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGAc                                                                                                      >  1:361054/1‑150 (MQ=255)
                                                tCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGACTTAATATGAAACTGCAATAAATTCTTTAATGTGCTACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGAcc                                                                                                     >  1:556430/1‑149 (MQ=255)
                                                   gTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAt                                                                                                  >  1:678762/1‑150 (MQ=255)
                                                   gTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAt                                                                                                  <  1:520499/150‑1 (MQ=255)
                                                      aaaTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTa                                                                                               <  1:948377/150‑1 (MQ=255)
                                                       aaTTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTaa                                                                                              <  1:211799/150‑1 (MQ=255)
                                                          tGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAATCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATAc                                                                                           >  1:513715/1‑150 (MQ=255)
                                                           gAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACt                                                                                          <  1:2494191/150‑1 (MQ=255)
                                                           gAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACt                                                                                          >  2:2351342/1‑150 (MQ=255)
                                                           gAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACt                                                                                          >  1:2024513/1‑150 (MQ=255)
                                                            aGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACtt                                                                                         <  1:4242622/150‑1 (MQ=255)
                                                                    gCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAATTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAt                                                                                 <  1:3600158/150‑1 (MQ=255)
                                                                    gCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAACGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAt                                                                                 <  1:4563380/150‑1 (MQ=255)
                                                                     ccGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATg                                                                                <  2:3056716/150‑1 (MQ=255)
                                                                      cGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGa                                                                               <  2:2388181/150‑1 (MQ=255)
                                                                          cGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttgg                                                                           <  2:3980432/150‑1 (MQ=255)
                                                                             ttCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGAttggttg                                                                        >  1:188821/1‑150 (MQ=255)
                                                                                aTCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATtggttggta                                                                     <  1:2330256/150‑2 (MQ=255)
                                                                                    cTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGttt                                                                 <  1:4114566/150‑1 (MQ=255)
                                                                                     tGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTa                                                                <  1:3768328/150‑1 (MQ=255)
                                                                                        cAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACAt                                                             <  2:743855/150‑1 (MQ=255)
                                                                                             aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAaca                                                        >  1:3874235/1‑150 (MQ=255)
                                                                                              gAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAacac                                                       >  1:3652323/1‑150 (MQ=255)
                                                                                              gAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAGGATTGGTTGGTCGTTTACATAAacac                                                       >  1:2939608/1‑150 (MQ=255)
                                                                                               aaCGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACt                                                      >  2:3676800/1‑150 (MQ=255)
                                                                                                aCGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTg                                                     <  1:1623507/150‑1 (MQ=255)
                                                                                                 cGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGa                                                    <  2:4329522/150‑1 (MQ=255)
                                                                                                 cGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGa                                                    <  1:1593067/150‑1 (MQ=255)
                                                                                                   gCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcg                                                  >  2:3127948/1‑150 (MQ=255)
                                                                                                    cTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTAATTTTTACTTCAATTTAGACAAATTTAATAATTTGGTTAATGTTTGGTTGGTCGTTTAAAAAAacaaatacgt                                                 >  1:841818/1‑143 (MQ=255)
                                                                                                      ttttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgtcg                                               <  1:3080231/150‑1 (MQ=255)
                                                                                                       tttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAcgtcgt                                              >  2:3787970/1‑150 (MQ=255)
                                                                                                          cGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTaaa                                           <  1:1914712/150‑1 (MQ=255)
                                                                                                           gTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAac                                          <  1:4175421/150‑1 (MQ=255)
                                                                                                           gTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAac                                          >  2:2539028/1‑150 (MQ=255)
                                                                                                           gTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTGTACATAAACACTGACGTCGTAAac                                          >  1:1837316/1‑150 (MQ=255)
                                                                                                            ttatttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCAGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTCATACTTTGGTTAATGATGGGTTGGTCGGTTACATAAACACGGACGTCGTAAaca                                         >  2:2555503/1‑150 (MQ=255)
                                                                                                             tatttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTAAATAAACACTGACGTCGTAAacac                                        >  1:3214939/1‑150 (MQ=255)
                                                                                                              atttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACAcc                                       <  2:524307/150‑1 (MQ=255)
                                                                                                              atttatATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACAcc                                       <  1:4215377/150‑1 (MQ=255)
                                                                                                               tttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGACTGGGCGTACTTTTTTCCATCAAAAGCGACAAATGAAATAATTTGGTGAATGATTGGTTGTTCGTTTACATAAAAACTGACGTCGTAAACAacc                                      >  1:3818610/1‑150 (MQ=255)
                                                                                                                   tatCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGta                                  >  1:4596605/1‑150 (MQ=255)
                                                                                                                   tatCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGta                                  >  2:1161640/1‑150 (MQ=255)
                                                                                                                    atCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGtat                                 <  1:686350/150‑1 (MQ=255)
                                                                                                                     tCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGtata                                >  1:4566964/1‑150 (MQ=255)
                                                                                                                       gggAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGc                              <  1:288718/150‑1 (MQ=255)
                                                                                                                       gggAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGc                              >  2:1659086/1‑150 (MQ=255)
                                                                                                                          agaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCggg                           >  2:206363/1‑150 (MQ=255)
                                                                                                                          agaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCggg                           >  2:206419/1‑150 (MQ=255)
                                                                                                                           gaATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgggg                          >  2:4633674/1‑150 (MQ=255)
                                                                                                                           gaATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgggg                          >  2:3569669/1‑150 (MQ=255)
                                                                                                                             aTTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGtt                        >  2:3894974/1‑150 (MQ=255)
                                                                                                                               tAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTAc                      >  2:1270644/1‑150 (MQ=255)
                                                                                                                                aaTATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTAcc                     >  2:1032796/1‑150 (MQ=255)
                                                                                                                                 atatGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCt                    >  1:3234904/1‑150 (MQ=255)
                                                                                                                                   atGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTc                  <  1:2463658/150‑1 (MQ=255)
                                                                                                                                     gAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCAc                >  2:1339515/1‑150 (MQ=255)
                                                                                                                                     gAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCAc                >  1:761512/1‑150 (MQ=255)
                                                                                                                                      aaaCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACt               >  1:4575725/1‑150 (MQ=255)
                                                                                                                                      aaaCTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACt               >  2:2109802/1‑150 (MQ=255)
                                                                                                                                        aCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGt             >  1:4456707/1‑150 (MQ=255)
                                                                                                                                        aCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGt             <  1:1254763/150‑1 (MQ=255)
                                                                                                                                         cTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTa            >  1:169892/1‑150 (MQ=255)
                                                                                                                                               tAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGGCGTTTTCATAAAAACTGAAGTCGTAAAAACCCGGTATAGCGGGGTTACCATCACTGTACCGGCt      >  1:1735996/1‑150 (MQ=255)
                                                                                                                                               tAAATTATTTAATGTGATACAAGCCTGGGCGTTATTTTTTCCTTAAAATGCGACAAATTTAATACTTTTGTTAATGATTGGTTGGTCGTTTAAATAAAAACTGACGTCGTAAAAACCCGGTATAGCGGGGTTAACTTAACTGTACCCGCt      >  1:1735330/1‑150 (MQ=255)
                                                                                                                                                 aaTTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTga    >  2:1599411/1‑150 (MQ=255)
                                                                                                                                                   ttCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgaga  <  1:2637608/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGA  >  CP009273/151471‑151767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGA  >  CP009273/151473‑151767
                                                                                                                                                   |                                                                                                                                                   
ACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATT                                                                                                                                                   <  SRR19973073.2309466/150‑1 (MQ=60)
 CGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTT                                                                                                                                                  <  SRR19973073.3023490/150‑1 (MQ=60)
  GTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTA                                                                                                                                                 <  SRR19973073.3238686/150‑1 (MQ=60)
  GTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTA                                                                                                                                                 <  SRR19973073.3238726/150‑1 (MQ=60)
  GTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTA                                                                                                                                                 <  SRR19973073.4604491/150‑1 (MQ=60)
       TCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTG                                                                                                                                            <  SRR19973073.2326116/150‑1 (MQ=60)
         GTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGAT                                                                                                                                          <  SRR19973073.4633707/150‑1 (MQ=60)
         GTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGAT                                                                                                                                          >  SRR19973073.4547622/1‑150 (MQ=60)
         GTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGAT                                                                                                                                          >  SRR19973073.4547569/1‑150 (MQ=60)
         GTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGAT                                                                                                                                          <  SRR19973073.1552751/150‑1 (MQ=60)
          TTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATA                                                                                                                                         >  SRR19973073.3399339/1‑150 (MQ=60)
                    AACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGG                                                                                                                               >  SRR19973073.3135317/1‑150 (MQ=60)
                    AACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGG                                                                                                                               <  SRR19973073.650604/150‑1 (MQ=60)
                       GAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGT                                                                                                                            >  SRR19973073.3673353/1‑150 (MQ=60)
                       GAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGT                                                                                                                            >  SRR19973073.3673158/1‑150 (MQ=60)
                        AACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTT                                                                                                                           <  SRR19973073.2094389/150‑1 (MQ=60)
                         ACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTC                                                                                                                          <  SRR19973073.4355904/150‑1 (MQ=60)
                         ACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTC                                                                                                                          >  SRR19973073.1482570/1‑150 (MQ=60)
                           AGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTT                                                                                                                        <  SRR19973073.3226607/150‑1 (MQ=60)
                           AGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTT                                                                                                                        >  SRR19973073.1686206/1‑150 (MQ=60)
                             AAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTT                                                                                                                      <  SRR19973073.1610656/150‑1 (MQ=60)
                             AAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTT                                                                                                                      <  SRR19973073.2679695/150‑1 (MQ=60)
                             AAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTT                                                                                                                      <  SRR19973073.3938930/150‑1 (MQ=60)
                              AGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTT                                                                                                                     <  SRR19973073.3397503/150‑1 (MQ=60)
                               GCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTT                                                                                                                    <  SRR19973073.440543/150‑1 (MQ=60)
                                CCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTC                                                                                                                   >  SRR19973073.226656/1‑150 (MQ=60)
                                 CTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCC                                                                                                                  <  SRR19973073.4494743/150‑1 (MQ=60)
                                  TGAGGGNTAATTTCCNTCAAATTGNGGCAATTGNCGAGCGTTTNATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCT                                                                                                                 <  SRR19973073.3102153/150‑1 (MQ=60)
                                  TGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCT                                                                                                                 <  SRR19973073.2800120/150‑1 (MQ=60)
                                   GAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTACTT                                                                                                                >  SRR19973073.4027507/1‑150 (MQ=60)
                                        ATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATT                                                                                                           >  SRR19973073.1905103/1‑150 (MQ=60)
                                         TAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTG                                                                                                          <  SRR19973073.1188468/150‑1 (MQ=60)
                                           ATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCG                                                                                                        <  SRR19973073.2052264/150‑1 (MQ=60)
                                             TTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGAC                                                                                                      >  SRR19973073.2999704/1‑150 (MQ=60)
                                             TTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGAC                                                                                                      >  SRR19973073.361777/1‑150 (MQ=60)
                                             TTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGAC                                                                                                      >  SRR19973073.361445/1‑150 (MQ=60)
                                              TCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGACTTAATATGAAACTGCAATAAATTCTTTAATGTGCTACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACC                                                                                                     >  SRR19973073.557630/1‑150 (MQ=60)
                                                 GTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAT                                                                                                  <  SRR19973073.521623/150‑1 (MQ=60)
                                                 GTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAT                                                                                                  <  SRR19973073.3454986/150‑1 (MQ=60)
                                                 GTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAT                                                                                                  >  SRR19973073.680177/1‑150 (MQ=60)
                                                 GTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAT                                                                                                  >  SRR19973073.680175/1‑150 (MQ=60)
                                                 GTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAAT                                                                                                  <  SRR19973073.521575/150‑1 (MQ=60)
                                                   CAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGT                                                                                                <  SRR19973073.2043358/150‑1 (MQ=60)
                                                    AAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTA                                                                                               <  SRR19973073.950352/150‑1 (MQ=60)
                                                     AATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAA                                                                                              <  SRR19973073.212298/150‑1 (MQ=60)
                                                        TGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAATCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATAC                                                                                           >  SRR19973073.514818/1‑150 (MQ=60)
                                                         GAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACT                                                                                          <  SRR19973073.2499345/150‑1 (MQ=60)
                                                         GAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACT                                                                                          >  SRR19973073.2028532/1‑150 (MQ=60)
                                                         GAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACT                                                                                          >  SRR19973073.2028720/1‑150 (MQ=60)
                                                          AGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTT                                                                                         <  SRR19973073.4251020/150‑1 (MQ=60)
                                                             CAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACATTGG                                                                                      >  SRR19973073.2782112/1‑150 (MQ=60)
                                                                  GCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAATTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAT                                                                                 <  SRR19973073.3607381/150‑1 (MQ=60)
                                                                  GCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAACGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAT                                                                                 <  SRR19973073.4572515/150‑1 (MQ=60)
                                                                   CCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATG                                                                                >  SRR19973073.2911061/1‑150 (MQ=60)
                                                                      AGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATT                                                                             <  SRR19973073.2607697/150‑1 (MQ=60)
                                                                           TTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTG                                                                        <  SRR19973073.3330944/150‑1 (MQ=60)
                                                                           TTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTTATACAATCCTGGGCGTTCATTTTTACTTAAATTTCGACAAATGTAATAATTTGGTTAATGATTTGTTG                                                                        >  SRR19973073.572349/1‑150 (MQ=60)
                                                                           TTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTG                                                                        >  SRR19973073.189264/1‑150 (MQ=60)
                                                                             CATCCCTGGCAAGCAGAACGGCTTTTTAGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGAGATAAAAGCCTGGGCGATCTTTTTTACTTAAAATGAGAAAAATCTAATAATTTGGTTAATGATTtttttgt                                                                      >  SRR19973073.282902/1‑143 (MQ=60)
                                                                              ATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTA                                                                     <  SRR19973073.2335010/150‑1 (MQ=60)
                                                                                  CTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTT                                                                 <  SRR19973073.4122801/150‑1 (MQ=60)
                                                                                   TGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTA                                                                <  SRR19973073.3775883/150‑1 (MQ=60)
                                                                                    GGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTAC                                                               <  SRR19973073.3052975/150‑1 (MQ=60)
                                                                                        AGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAA                                                           >  SRR19973073.1872278/1‑150 (MQ=60)
                                                                                        AGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAA                                                           >  SRR19973073.4563935/1‑150 (MQ=60)
                                                                                          CAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAAC                                                         <  SRR19973073.730975/150‑1 (MQ=60)
                                                                                          CAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAAC                                                         >  SRR19973073.4341297/1‑150 (MQ=60)
                                                                                           AGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACA                                                        >  SRR19973073.3881913/1‑150 (MQ=60)
                                                                                            GAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACAC                                                       <  SRR19973073.525718/150‑1 (MQ=60)
                                                                                            GAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACAC                                                       >  SRR19973073.3659655/1‑150 (MQ=60)
                                                                                            GAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACAC                                                       >  SRR19973073.3659650/1‑150 (MQ=60)
                                                                                            GAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAAGGATTGGTTGGTCGTTTACATAAACAC                                                       >  SRR19973073.2945613/1‑150 (MQ=60)
                                                                                              ACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTG                                                     <  SRR19973073.1626858/150‑1 (MQ=60)
                                                                                               CGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGA                                                    >  SRR19973073.4578846/1‑150 (MQ=60)
                                                                                               CGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGA                                                    <  SRR19973073.1596357/150‑1 (MQ=60)
                                                                                                GGCTTTTTCGTTATTTATATCGTGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAC                                                   <  SRR19973073.858568/150‑1 (MQ=60)
                                                                                                GGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAC                                                   >  SRR19973073.3861495/1‑150 (MQ=60)
                                                                                                  CTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTAATTTTTACTTCAATTTAGACAAATTTAATAATTTGGTTAATGTTTGGTTGGTCGTTTAaaaaaacaaatacgt                                                 >  SRR19973073.843553/1‑135 (MQ=60)
                                                                                                    TTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCG                                               <  SRR19973073.3086415/150‑1 (MQ=60)
                                                                                                        CGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAA                                           <  SRR19973073.1918613/150‑1 (MQ=60)
                                                                                                         GTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAAC                                          <  SRR19973073.4183720/150‑1 (MQ=60)
                                                                                                         GTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAAC                                          <  SRR19973073.4183735/150‑1 (MQ=60)
                                                                                                         GTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAAC                                          <  SRR19973073.4183791/150‑1 (MQ=60)
                                                                                                         GTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTGTACATAAACACTGACGTCGTAAAC                                          >  SRR19973073.1841100/1‑150 (MQ=60)
                                                                                                           TATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTAAATAAACACTGACGTCGTAAACAC                                        >  SRR19973073.3221427/1‑150 (MQ=60)
                                                                                                           TATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATAATTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGAAGTCGTAAACAC                                        >  SRR19973073.2448230/1‑150 (MQ=60)
                                                                                                            ATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACC                                       <  SRR19973073.4223744/150‑1 (MQ=60)
                                                                                                            ATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACC                                       <  SRR19973073.4223888/150‑1 (MQ=60)
                                                                                                             TTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCC                                      >  SRR19973073.3826104/1‑150 (MQ=60)
                                                                                                             TTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGACTGGGCGTACTTTTTTCCATCAAAAGCGACAAATGAAATAATTTGGTGAATGATTGGTTGTTCGTTTACATAAAAACTGACGTCGTAAACAACC                                      >  SRR19973073.3826216/1‑150 (MQ=60)
                                                                                                                ATATCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGT                                   >  SRR19973073.3670031/1‑150 (MQ=60)
                                                                                                                 TATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTA                                  >  SRR19973073.4605789/1‑150 (MQ=60)
                                                                                                                  ATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTAT                                 <  SRR19973073.1811251/150‑1 (MQ=60)
                                                                                                                  ATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTAT                                 <  SRR19973073.687778/150‑1 (MQ=60)
                                                                                                                  ATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTAT                                 <  SRR19973073.1811235/150‑1 (MQ=60)
                                                                                                                   TCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATA                                >  SRR19973073.4576100/1‑150 (MQ=60)
                                                                                                                   TCGGGAGAATTAATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATA                                >  SRR19973073.4576103/1‑150 (MQ=60)
                                                                                                                     GGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGC                              <  SRR19973073.289339/150‑1 (MQ=60)
                                                                                                                               ATATGAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCT                    >  SRR19973073.3241430/1‑150 (MQ=60)
                                                                                                                                 ATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTC                  <  SRR19973073.2468756/150‑1 (MQ=60)
                                                                                                                                   GAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCAC                >  SRR19973073.2907419/1‑150 (MQ=60)
                                                                                                                                   GAAACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCAC                >  SRR19973073.763043/1‑150 (MQ=60)
                                                                                                                                    AAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACT               >  SRR19973073.1261182/1‑150 (MQ=60)
                                                                                                                                    AAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACT               >  SRR19973073.4584874/1‑150 (MQ=60)
                                                                                                                                     AACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTG              >  SRR19973073.2470238/1‑150 (MQ=60)
                                                                                                                                      ACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGT             <  SRR19973073.1257384/150‑1 (MQ=60)
                                                                                                                                      ACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGT             >  SRR19973073.4465662/1‑150 (MQ=60)
                                                                                                                                      ACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGT             <  SRR19973073.1257385/150‑1 (MQ=60)
                                                                                                                                      ACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTAAATTTCGACAAATGTAATACTTTGGTTAATTATTTGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGGATAGCGGGGTTACCTTCACTGG             >  SRR19973073.2133502/1‑150 (MQ=60)
                                                                                                                                      ACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGT             <  SRR19973073.77963/150‑1 (MQ=60)
                                                                                                                                      ACTGCAATAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCATCAATTTCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAAAACAGACGTCGGAAACACCCGGTATAGCGGGGTTACCTTCAATGT             >  SRR19973073.976315/1‑150 (MQ=60)
                                                                                                                                       CTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTA            >  SRR19973073.170293/1‑150 (MQ=60)
                                                                                                                                       CTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGGCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTA            >  SRR19973073.170348/1‑150 (MQ=60)
                                                                                                                                         GCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACC          <  SRR19973073.2666326/150‑1 (MQ=60)
                                                                                                                                             TAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTGCTTCAATTGCGACAAATGTAATACGTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTTACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCT      >  SRR19973073.1738948/1‑150 (MQ=60)
                                                                                                                                             TAAATTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGGCGTTTTCATAAAAACTGAAGTCGTAAAAACCCGGTATAGCGGGGTTACCATCACTGTACCGGCT      >  SRR19973073.1739604/1‑150 (MQ=60)
                                                                                                                                             TAAATTATTTAATGTGATACAAGCCTGGGCGTTATTTTTTCCTTAAAATGCGACAAATTTAATACTTTTGTTAATGATTGGTTGGTCGTTTAAATAAAAACTGACGTCGTAAAAACCCGGTATAGCGGGGTTAACTTAACTGTACCCGCT      >  SRR19973073.1738936/1‑150 (MQ=60)
                                                                                                                                               AATTATTTAATGTGATACAAGCCTGGGCGTTAATTTTTCCTTCAAatgcgtcaaaggtaaaaattttgttaaagaattgttggtcgtttaaaaaaaaaaagaattagtaaaaaaccggtaaagcggggttacaatcacagtaacgcctta    >  SRR19973073.3434303/1‑45 (MQ=50)
                                                                                                                                                 TTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGA  <  SRR19973073.2643017/150‑1 (MQ=60)
                                                                                                                                                 TTATTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGA  >  SRR19973073.3015432/1‑150 (MQ=60)
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ACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATCCCTGGCAAGCAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGA  >  CP009273/151473‑151767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑