Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F224 I0 R1	1457	284.0	9290224	95.7%	8890744	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	176,783	G→T	89.4%	D354Y (GAT→TAT)	dgt →	deoxyguanosine triphosphate triphosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	176,783	0	G	T	89.4%	656.2 / 76.4	243	D354Y (GAT→TAT)	dgt	deoxyguanosine triphosphate triphosphohydrolase
Reads supporting (aligned to +/- strand): ref base G (12/14); new base T (100/117); total (112/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

GGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG  >  CP009273/176636‑176926

 gTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTtata                                                                                                                                              >  1:1029005/1‑150 (MQ=255)

 gTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTtata                                                                                                                                              >  1:3352262/1‑150 (MQ=255)

 gTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTtata                                                                                                                                              >  2:1257940/1‑150 (MQ=255)

    cGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATc                                                                                                                                           <  1:519539/150‑1 (MQ=255)

     gCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATct                                                                                                                                          >  1:3673508/1‑150 (MQ=255)

       tctTTTCGCTTGTGGTTGAAAATGCCTGGGAAAAATCATTCTCAAATAGTTTAAGACGCAGTACGGTAGATCTTTTTTTTATGTATTTACGGGTAAACACCCTTAATTTACTGGTACCCTACGCGGCACTACGATTTATTTATATTctgc                                                                                                                                        <  2:328638/150‑1 (MQ=255)

       tctTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgc                                                                                                                                        <  2:1205275/150‑1 (MQ=255)

          tttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcctg                                                                                                                                     <  2:1972111/150‑1 (MQ=255)

          tttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcctg                                                                                                                                     <  2:4311485/150‑1 (MQ=255)

            tCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCg                                                                                                                                   >  1:761318/1‑150 (MQ=255)

             cGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGa                                                                                                                                  >  2:2347081/1‑150 (MQ=255)

             cGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGa                                                                                                                                  >  2:116593/1‑150 (MQ=255)

              gCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAt                                                                                                                                 >  2:809839/1‑150 (MQ=255)

               cTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAtt                                                                                                                                >  2:1067975/1‑150 (MQ=255)

                tggtggTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAttt                                                                                                                               <  2:2842343/150‑1 (MQ=255)

                tggtggTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAttt                                                                                                                               >  1:3035932/1‑150 (MQ=255)

                tggtggTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAttt                                                                                                                               <  2:3450675/150‑1 (MQ=255)

                  gtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTc                                                                                                                             <  1:79164/150‑1 (MQ=255)

                            aTGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGt                                                                                                                   <  1:1213576/150‑1 (MQ=255)

                             tGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACTGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGtt                                                                                                                  <  1:1722917/150‑1 (MQ=255)

                             tGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGtt                                                                                                                  <  2:3448480/150‑1 (MQ=255)

                               ccTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTa                                                                                                                >  1:2178405/1‑150 (MQ=255)

                               ccTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTa                                                                                                                >  1:4088469/1‑150 (MQ=255)

                                cTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGTTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTaa                                                                                                               >  2:3280605/1‑150 (MQ=255)

                                 tGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAt                                                                                                              <  1:1325262/150‑1 (MQ=255)

                                 tGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAt                                                                                                              >  1:3998186/1‑150 (MQ=255)

                                    gAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCAt                                                                                                           <  1:1178115/150‑1 (MQ=255)

                                     aaaaaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATg                                                                                                          <  2:4388416/150‑1 (MQ=255)

                                       aaaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCa                                                                                                        >  1:3726640/1‑150 (MQ=255)

                                         aTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCatt                                                                                                      >  2:704322/1‑150 (MQ=255)

                                           cACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattat                                                                                                    <  1:4246071/150‑1 (MQ=255)

                                            aCGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattatt                                                                                                   >  1:489147/1‑150 (MQ=255)

                                            aCGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattatt                                                                                                   <  2:2276588/150‑1 (MQ=255)

                                                tCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGaa                                                                                               >  2:4029707/1‑150 (MQ=255)

                                                tCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGaa                                                                                               <  2:46889/150‑1 (MQ=255)

                                                  aaaTAGTTTAAGCCGCAGTACGGAAGATCTGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGa                                                                                             <  2:3014120/150‑1 (MQ=255)

                                                  aaaTAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGa                                                                                             >  2:4291409/1‑150 (MQ=255)

                                                     tAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGc                                                                                          <  1:4588639/150‑1 (MQ=255)

                                                      aGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGcc                                                                                         <  2:84854/150‑1 (MQ=255)

                                                       gTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCa                                                                                        <  2:2658855/150‑1 (MQ=255)

                                                          tAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCg                                                                                     >  1:280432/1‑150 (MQ=255)

                                                           aaGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGa                                                                                    <  1:1911829/150‑1 (MQ=255)

                                                           aaGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCAGTATTGGAAGATGCCAGCGa                                                                                    >  2:3707873/1‑150 (MQ=255)

                                                             gCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAAt                                                                                  <  2:948807/150‑1 (MQ=255)

                                                              ccGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATg                                                                                 <  1:1801735/150‑1 (MQ=255)

                                                              ccGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATg                                                                                 <  2:1381696/150‑1 (MQ=255)

                                                              ccGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATg                                                                                 <  2:1923564/150‑1 (MQ=255)

                                                                gCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCa                                                                               <  2:2591222/150‑1 (MQ=255)

                                                                  aGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGc                                                                             <  1:4078850/150‑1 (MQ=255)

                                                                    tACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGa                                                                           >  2:1133515/1‑150 (MQ=255)

                                                                      cGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtc                                                                         <  1:918281/150‑1 (MQ=255)

                                                                       ggAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                        >  2:1987905/1‑150 (MQ=255)

                                                                          aGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttct                                                                     >  1:385638/1‑150 (MQ=255)

                                                                           gATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATcttctt                                                                    >  2:1084656/1‑150 (MQ=255)

                                                                            aTCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTa                                                                   >  1:4519154/1‑150 (MQ=255)

                                                                              cAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAg                                                                 <  2:767683/150‑1 (MQ=255)

                                                                              cAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCAGTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAg                                                                 >  2:1685292/1‑150 (MQ=255)

                                                                               aGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGc                                                                >  1:3492633/1‑150 (MQ=255)

                                                                                 ttttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCta                                                              <  1:3538916/150‑1 (MQ=255)

                                                                                  tttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtat                                                             <  2:1825706/150‑1 (MQ=255)

                                                                                   ttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtata                                                            >  2:2737410/1‑150 (MQ=255)

                                                                                   ttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGAATTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTTGAAGATGCCATCGAATGCAGCGATCTTCTTAAGCaata                                                            >  1:3788808/1‑150 (MQ=255)

                                                                                    tttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtatat                                                           <  2:2336614/150‑1 (MQ=255)

                                                                                      tATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATaa                                                         <  2:2537260/150‑1 (MQ=255)

                                                                                       aTGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATaaa                                                        >  2:1507694/1‑150 (MQ=255)

                                                                                        tGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGTTATATaaaa                                                       >  1:3402866/1‑150 (MQ=255)

                                                                                           aTTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATg                                                    <  1:765277/150‑1 (MQ=255)

                                                                                           aTTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATg                                                    >  2:3078038/1‑150 (MQ=255)

                                                                                             ttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTc                                                  <  1:1859410/150‑1 (MQ=255)

                                                                                             ttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTc                                                  <  1:2077129/150‑1 (MQ=255)

                                                                                             ttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTc                                                  <  1:4419571/150‑1 (MQ=255)

                                                                                                     aaaCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTaaa                                          >  2:571809/1‑150 (MQ=255)

                                                                                                        cacCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACa                                       <  1:2847241/150‑1 (MQ=255)

                                                                                                        cacCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACa                                       >  1:3086826/1‑150 (MQ=255)

                                                                                                         acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                      <  2:3352262/150‑1 (MQ=255)

                                                                                                         acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                      <  1:1516709/150‑1 (MQ=255)

                                                                                                         acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                      <  1:3159192/150‑1 (MQ=255)

                                                                                                         acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                      <  1:3159273/150‑1 (MQ=255)

                                                                                                         acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                      <  2:1029005/150‑1 (MQ=255)

                                                                                                         acCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAt                                      >  2:1884774/1‑150 (MQ=255)

                                                                                                           ccTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGTTATATAAAAATGTCGCTGTAAAACAtgt                                    <  2:531329/150‑1 (MQ=255)

                                                                                                           ccTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAtgt                                    <  1:2723263/150‑1 (MQ=255)

                                                                                                             taaataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATgtgt                                  >  1:2052176/1‑150 (MQ=255)

                                                                                                               aataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGttt                                >  2:3198985/1‑150 (MQ=255)

                                                                                                               aataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGttt                                <  2:2493400/150‑1 (MQ=255)

                                                                                                                ataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTa                               <  2:2482415/150‑1 (MQ=255)

                                                                                                                   aaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGcc                            >  1:4350580/1‑150 (MQ=255)

                                                                                                                   aaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGcc                            >  1:1955866/1‑150 (MQ=255)

                                                                                                                    aCTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCa                           <  1:1995665/150‑1 (MQ=255)

                                                                                                                      tGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATc                         <  2:230637/150‑1 (MQ=255)

                                                                                                                           cccTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGAt                    >  1:1631962/1‑150 (MQ=255)

                                                                                                                            ccTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATg                   <  2:662014/150‑1 (MQ=255)

                                                                                                                               aCGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCg                <  2:3673508/150‑1 (MQ=255)

                                                                                                                                 gcgGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAg              >  2:658947/1‑150 (MQ=255)

                                                                                                                                      acaACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCt         >  2:2447166/1‑150 (MQ=255)

                                                                                                                                       caACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCtt        >  2:4189264/1‑150 (MQ=255)

                                                                                                                                         aCGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGa      <  1:2603272/150‑1 (MQ=255)

                                                                                                                                             tttatttaTAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTg  >  1:1099988/1‑150 (MQ=255)

                                                                                                                                             tttatttaTAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTg  >  2:4397291/1‑150 (MQ=255)

                                                                                                                                             tttatttaTAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTg  >  2:3898156/1‑150 (MQ=255)

GGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG  >  CP009273/176636‑176926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGG  >  CP009273/176634‑176930

AAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTT                                                                                                                                                     >  SRR19973072.495267/1‑150 (MQ=60)

  GGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTAT                                                                                                                                                   <  SRR19973072.595105/150‑1 (MQ=60)

   GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATA                                                                                                                                                  >  SRR19973072.1031234/1‑150 (MQ=60)

   GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATA                                                                                                                                                  >  SRR19973072.3359371/1‑150 (MQ=60)

      CGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATC                                                                                                                                               <  SRR19973072.520779/150‑1 (MQ=60)

       GCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCT                                                                                                                                              >  SRR19973072.3323043/1‑150 (MQ=60)

       GCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCT                                                                                                                                              >  SRR19973072.3681251/1‑150 (MQ=60)

            TTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTG                                                                                                                                         <  SRR19973072.3136267/150‑1 (MQ=60)

              TCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCG                                                                                                                                       >  SRR19973072.763422/1‑150 (MQ=60)

              TCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCG                                                                                                                                       >  SRR19973072.762927/1‑150 (MQ=60)

               CGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGA                                                                                                                                      >  SRR19973072.2039309/1‑150 (MQ=60)

                  TGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTT                                                                                                                                   >  SRR19973072.3042363/1‑150 (MQ=60)

                  TGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTT                                                                                                                                   >  SRR19973072.1899499/1‑150 (MQ=60)

                   GGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTT                                                                                                                                  <  SRR19973072.367604/150‑1 (MQ=60)

                   GGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTT                                                                                                                                  <  SRR19973072.1795677/150‑1 (MQ=60)

                    GTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTC                                                                                                                                 <  SRR19973072.1282023/150‑1 (MQ=60)

                    GTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTC                                                                                                                                 <  SRR19973072.92428/150‑1 (MQ=60)

                    GTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTC                                                                                                                                 <  SRR19973072.79182/150‑1 (MQ=60)

                    GTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTC                                                                                                                                 <  SRR19973072.79366/150‑1 (MQ=60)

                       GTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCC                                                                                                                              <  SRR19973072.3940358/150‑1 (MQ=60)

                         TGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGG                                                                                                                            >  SRR19973072.1150866/1‑150 (MQ=60)

                            AAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAAC                                                                                                                         <  SRR19973072.1808660/150‑1 (MQ=60)

                              ATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGT                                                                                                                       <  SRR19973072.1216166/150‑1 (MQ=60)

                              ATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGT                                                                                                                       <  SRR19973072.1216170/150‑1 (MQ=60)

                              ATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGT                                                                                                                       <  SRR19973072.743412/150‑1 (MQ=60)

                               TGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACTGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTT                                                                                                                      <  SRR19973072.1726692/150‑1 (MQ=60)

                                 CCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTA                                                                                                                    >  SRR19973072.2183149/1‑150 (MQ=60)

                                 CCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTA                                                                                                                    >  SRR19973072.4097045/1‑150 (MQ=60)

                                 CCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTA                                                                                                                    >  SRR19973072.4097039/1‑150 (MQ=60)

                                 CCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTA                                                                                                                    >  SRR19973072.4097003/1‑150 (MQ=60)

                                   TGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAT                                                                                                                  >  SRR19973072.4006559/1‑150 (MQ=60)

                                   TGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAT                                                                                                                  <  SRR19973072.1328159/150‑1 (MQ=60)

                                      GAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCAT                                                                                                               <  SRR19973072.1180591/150‑1 (MQ=60)

                                       AAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATG                                                                                                              <  SRR19973072.1298367/150‑1 (MQ=60)

                                        AAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGC                                                                                                             <  SRR19973072.3804806/150‑1 (MQ=60)

                                         AAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCA                                                                                                            >  SRR19973072.3734509/1‑150 (MQ=60)

                                         AAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCA                                                                                                            >  SRR19973072.3734482/1‑150 (MQ=60)

                                          AATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCAT                                                                                                           <  SRR19973072.1992978/150‑1 (MQ=60)

                                             CACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTAT                                                                                                        <  SRR19973072.4254905/150‑1 (MQ=60)

                                              ACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATT                                                                                                       >  SRR19973072.490304/1‑150 (MQ=60)

                                              ACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATT                                                                                                       >  SRR19973072.490326/1‑150 (MQ=60)

                                                GCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGG                                                                                                     >  SRR19973072.4030211/1‑150 (MQ=60)

                                                       TAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGC                                                                                              <  SRR19973072.4598276/150‑1 (MQ=60)

                                                           TTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGC                                                                                          >  SRR19973072.103995/1‑150 (MQ=60)

                                                            TAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCG                                                                                         >  SRR19973072.281074/1‑150 (MQ=60)

                                                            TAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCG                                                                                         >  SRR19973072.281088/1‑150 (MQ=60)

                                                             AAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGA                                                                                        <  SRR19973072.1915901/150‑1 (MQ=60)

                                                                CCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATG                                                                                     <  SRR19973072.1805673/150‑1 (MQ=60)

                                                                 CGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGC                                                                                    >  SRR19973072.4596380/1‑150 (MQ=60)

                                                                    AGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGC                                                                                 <  SRR19973072.4087408/150‑1 (MQ=60)

                                                                      TACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGA                                                                               >  SRR19973072.3232140/1‑150 (MQ=60)

                                                                      TACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGA                                                                               <  SRR19973072.172678/150‑1 (MQ=60)

                                                                        CGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATC                                                                             <  SRR19973072.920271/150‑1 (MQ=60)

                                                                          GAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTT                                                                           <  SRR19973072.3635847/150‑1 (MQ=60)

                                                                           AAGATCAGTTTTTTATGTATTTACGGGTAANCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTC                                                                          >  SRR19973072.4295891/1‑150 (MQ=60)

                                                                            AGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCT                                                                         >  SRR19973072.4485239/1‑150 (MQ=60)

                                                                            AGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCT                                                                         >  SRR19973072.386471/1‑150 (MQ=60)

                                                                              ATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTA                                                                       >  SRR19973072.4528693/1‑150 (MQ=60)

                                                                               TCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAA                                                                      >  SRR19973072.2186292/1‑150 (MQ=60)

                                                                                 AGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGC                                                                    <  SRR19973072.1919592/150‑1 (MQ=60)

                                                                                 AGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGC                                                                    >  SRR19973072.3499941/1‑150 (MQ=60)

                                                                                 AGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGC                                                                    >  SRR19973072.3499924/1‑150 (MQ=60)

                                                                                 AGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGC                                                                    >  SRR19973072.3422185/1‑150 (MQ=60)

                                                                                   TTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTA                                                                  <  SRR19973072.3546358/150‑1 (MQ=60)

                                                                                    TTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTAT                                                                 >  SRR19973072.1614029/1‑150 (MQ=60)

                                                                                     TTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGAATTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTTGAAGATGCCATCGAATGCAGCGATCTTCTTAAGCAATA                                                                >  SRR19973072.3796749/1‑150 (MQ=60)

                                                                                      TTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTTATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATAT                                                               <  SRR19973072.1906336/150‑1 (MQ=60)

                                                                                          TGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGTTATATAAAA                                                           >  SRR19973072.3410055/1‑150 (MQ=60)

                                                                                          TGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGTTATATAAAA                                                           >  SRR19973072.3410048/1‑150 (MQ=60)

                                                                                             ATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATG                                                        <  SRR19973072.766891/150‑1 (MQ=60)

                                                                                               TTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTC                                                      <  SRR19973072.1863429/150‑1 (MQ=60)

                                                                                               TTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTC                                                      <  SRR19973072.2081651/150‑1 (MQ=60)

                                                                                               TTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTC                                                      <  SRR19973072.4428874/150‑1 (MQ=60)

                                                                                                TACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCG                                                     <  SRR19973072.1994914/150‑1 (MQ=60)

                                                                                                   GGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTG                                                  >  SRR19973072.834021/1‑150 (MQ=60)

                                                                                                     GTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTA                                                >  SRR19973072.472448/1‑150 (MQ=60)

                                                                                                      TAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAA                                               >  SRR19973072.307089/1‑150 (MQ=60)

                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                           <  SRR19973072.2853329/150‑1 (MQ=60)

                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                           <  SRR19973072.1470550/150‑1 (MQ=60)

                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                           >  SRR19973072.3524324/1‑150 (MQ=60)

                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                           >  SRR19973072.3093331/1‑150 (MQ=60)

                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                           <  SRR19973072.4148795/150‑1 (MQ=60)

                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                           <  SRR19973072.3038497/150‑1 (MQ=60)

                                                                                                           ACCCTAAATAAACTGGTACCCTACGCTGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGATATATAAAAATGTCGCTGTAAAACAT                                          <  SRR19973072.2478840/150‑1 (MQ=60)

                                                                                                           ACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAT                                          <  SRR19973072.3165943/150‑1 (MQ=60)

                                                                                                           ACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAT                                          <  SRR19973072.1519932/150‑1 (MQ=60)

                                                                                                           ACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAT                                          <  SRR19973072.3165862/150‑1 (MQ=60)

                                                                                                           ACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAT                                          <  SRR19973072.3165898/150‑1 (MQ=60)

                                                                                                            CCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATG                                         >  SRR19973072.2841242/1‑150 (MQ=60)

                                                                                                             CCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGT                                        <  SRR19973072.2729128/150‑1 (MQ=60)

                                                                                                             CCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGT                                        <  SRR19973072.2729072/150‑1 (MQ=60)

                                                                                                               TAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGT                                      >  SRR19973072.2056643/1‑150 (MQ=60)

                                                                                                               TAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGCAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGT                                      >  SRR19973072.2692553/1‑150 (MQ=60)

                                                                                                                 AATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTT                                    <  SRR19973072.1538203/150‑1 (MQ=60)

                                                                                                                  ATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTA                                   <  SRR19973072.475733/150‑1 (MQ=60)

                                                                                                                    AAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTATTTAGC                                 <  SRR19973072.1171841/150‑1 (MQ=60)

                                                                                                                     AACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCC                                <  SRR19973072.3738087/150‑1 (MQ=60)

                                                                                                                     AACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCC                                >  SRR19973072.4359762/1‑150 (MQ=60)

                                                                                                                     AACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCC                                >  SRR19973072.4359698/1‑150 (MQ=60)

                                                                                                                     AACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCC                                >  SRR19973072.1960041/1‑150 (MQ=60)

                                                                                                                      ACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCA                               <  SRR19973072.1999964/150‑1 (MQ=60)

                                                                                                                          GTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCA                           >  SRR19973072.20756/1‑150 (MQ=60)

                                                                                                                          GTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCA                           >  SRR19973072.20737/1‑150 (MQ=60)

                                                                                                                             CCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGAT                        >  SRR19973072.1635521/1‑150 (MQ=60)

                                                                                                                             CACTACGCGTAACAACGATTTATTGATAATCTGCATGCGATTTTCGCCTGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTTAGTTATATTAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGAT                        <  SRR19973072.777269/150‑1 (MQ=60)

                                                                                                                               CTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGT                      >  SRR19973072.3877448/1‑150 (MQ=60)

                                                                                                                               CTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGT                      <  SRR19973072.3101097/150‑1 (MQ=60)

                                                                                                                               CTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGT                      <  SRR19973072.2948517/150‑1 (MQ=60)

                                                                                                                                TACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTC                     >  SRR19973072.4314853/1‑150 (MQ=60)

                                                                                                                                          AACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTG           >  SRR19973072.4482994/1‑150 (MQ=60)

                                                                                                                                          AACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTG           >  SRR19973072.369338/1‑150 (MQ=60)

                                                                                                                                          AACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTG           >  SRR19973072.2146231/1‑150 (MQ=60)

                                                                                                                                           ACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGA          <  SRR19973072.2608884/150‑1 (MQ=60)

                                                                                                                                             GATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAAT        <  SRR19973072.174090/150‑1 (MQ=60)

                                                                                                                                               TTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG      >  SRR19973072.1102317/1‑150 (MQ=60)

                                                                                                                                                   TTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGG  <  SRR19973072.1206551/150‑1 (MQ=60)

                                                                                                                                                   TTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGG  <  SRR19973072.3379411/150‑1 (MQ=60)

                                                                                                                                                   TTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGG  <  SRR19973072.1206420/150‑1 (MQ=60)

AAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGG  >  CP009273/176634‑176930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`