Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 176,783 G→T 100% D354Y (GAT→TAT)  dgt → deoxyguanosine triphosphate triphosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273176,7830GT99.5% 621.5 / ‑4.2 187D354Y (GAT→TAT) dgtdeoxyguanosine triphosphate triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (94/92);  total (95/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG  >  CP009273/176637‑176926
                                                                                                                                                  |                                                                                                                                               
 ttCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATaa                                                                                                                                             >  1:3430339/1‑150 (MQ=255)
   cGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATc                                                                                                                                           >  2:4570048/1‑150 (MQ=255)
   cGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATc                                                                                                                                           >  2:1561111/1‑150 (MQ=255)
      tctTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgc                                                                                                                                        >  1:4700469/1‑150 (MQ=255)
       ctTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcc                                                                                                                                       <  1:3708197/150‑1 (MQ=255)
         tttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGGAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcctg                                                                                                                                     <  2:2709325/150‑1 (MQ=255)
          ttCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATctgcctgc                                                                                                                                    <  2:4655491/150‑1 (MQ=255)
              cTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGAtt                                                                                                                                <  1:4020928/150‑1 (MQ=255)
                 gtggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTc                                                                                                                             >  1:2700034/1‑150 (MQ=255)
                   ggtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGc                                                                                                                           >  2:4160170/1‑150 (MQ=255)
                    gtTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGcc                                                                                                                          <  2:4957379/150‑1 (MQ=255)
                      tGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCgg                                                                                                                        >  2:488326/1‑150 (MQ=255)
                        aataTGACTGGGAAAAATCACGCTCAAATAGTTTAAGCAGCAGTACGGAAGATCAGTTTTTTATTTATTTTCGGGTAAACACCCTTAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGaa                                                                                                                      <  1:4867969/147‑1 (MQ=255)
                         aaaTGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAAc                                                                                                                     <  1:2059720/150‑1 (MQ=255)
                          aaTGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACg                                                                                                                    <  2:3252163/150‑1 (MQ=255)
                             gCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGttt                                                                                                                 <  1:3466195/150‑1 (MQ=255)
                                tGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAt                                                                                                              >  1:4574450/1‑150 (MQ=255)
                                tGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAt                                                                                                              <  2:3607042/150‑1 (MQ=255)
                                  ggAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCa                                                                                                            >  2:2916599/1‑150 (MQ=255)
                                     aaaaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGc                                                                                                         <  1:570242/150‑1 (MQ=255)
                                      aaaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCa                                                                                                        >  2:3094627/1‑150 (MQ=255)
                                       aaTCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCat                                                                                                       <  1:2747102/150‑1 (MQ=255)
                                          cACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTTGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattat                                                                                                    <  2:5160109/150‑1 (MQ=255)
                                          cACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattat                                                                                                    >  1:4365223/1‑150 (MQ=255)
                                          cACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCattat                                                                                                    <  1:2424267/150‑1 (MQ=255)
                                            cGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTg                                                                                                  >  2:1506120/1‑150 (MQ=255)
                                              cTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGa                                                                                                >  2:3574157/1‑150 (MQ=255)
                                                cAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGTAAg                                                                                              >  2:4057469/1‑150 (MQ=255)
                                                cAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAg                                                                                              >  1:4913657/1‑150 (MQ=255)
                                                     aGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGcc                                                                                         <  1:3746164/150‑1 (MQ=255)
                                                     aGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGcc                                                                                         <  1:1036010/150‑1 (MQ=255)
                                                      gTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGTTAAAAACCCTAAATAAAATGGTACACTACGCGGCACAAAGATTTATTTATAATCTGCCTGCGATTTTCGCAGGAACGATTAATCATGCATTATTGGTAGATGCCa                                                                                        >  1:905062/1‑150 (MQ=255)
                                                       tttAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAg                                                                                       <  2:3486983/150‑1 (MQ=255)
                                                          aaGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGa                                                                                    >  1:2708198/1‑150 (MQ=255)
                                                           aGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGaa                                                                                   >  2:1476713/1‑150 (MQ=255)
                                                           aGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACGGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGaa                                                                                   >  1:3009523/1‑150 (MQ=255)
                                                             ccGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATg                                                                                 <  1:1999257/150‑1 (MQ=255)
                                                               gCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCa                                                                               <  1:3739047/150‑1 (MQ=255)
                                                               gCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCa                                                                               >  1:2923546/1‑150 (MQ=255)
                                                                cAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCCGCACAACGATTTATTTATAATCTGCCTGCCATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATCCCAGCGAAAGCAg                                                                              >  1:4235066/1‑150 (MQ=255)
                                                                 aGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGc                                                                             >  1:3300301/1‑150 (MQ=255)
                                                                  gTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCg                                                                            <  1:4592160/150‑1 (MQ=255)
                                                                  gTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCg                                                                            <  2:2464597/150‑1 (MQ=255)
                                                                    aCGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAt                                                                          <  2:3495456/150‑1 (MQ=255)
                                                                    aCGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAt                                                                          <  2:236663/150‑1 (MQ=255)
                                                                    aCGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAt                                                                          <  2:221904/150‑1 (MQ=255)
                                                                     cGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtc                                                                         <  2:1740618/150‑1 (MQ=255)
                                                                      ggAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                        >  1:632014/1‑150 (MQ=255)
                                                                      ggAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                        >  2:3465603/1‑150 (MQ=255)
                                                                      ggAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                        >  2:2354447/1‑150 (MQ=255)
                                                                      ggAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtct                                                                        >  2:2057109/1‑150 (MQ=255)
                                                                       gAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCAAATGCAGCGAtctt                                                                       >  2:4535028/1‑150 (MQ=255)
                                                                        aaGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttc                                                                      <  2:2788537/150‑1 (MQ=255)
                                                                        aaGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttc                                                                      >  1:3409357/1‑150 (MQ=255)
                                                                         aGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttct                                                                     >  2:2984601/1‑150 (MQ=255)
                                                                         aGATCAGTTTTGTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAtcttct                                                                     <  2:2189658/150‑1 (MQ=255)
                                                                             cAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAg                                                                 >  1:2657271/1‑150 (MQ=255)
                                                                               gTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGTCAGCGAATGCAGCGATCTTCTTAAGCt                                                               >  2:3509654/1‑150 (MQ=255)
                                                                                ttttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCta                                                              >  2:1773540/1‑150 (MQ=255)
                                                                                 tttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtat                                                             >  1:3270301/1‑150 (MQ=255)
                                                                                 tttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtat                                                             >  1:1282410/1‑150 (MQ=255)
                                                                                 tttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtat                                                             <  1:202833/150‑1 (MQ=255)
                                                                                  ttttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtata                                                            <  2:2350223/150‑1 (MQ=255)
                                                                                   tttATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCtatat                                                           <  2:2662218/150‑1 (MQ=255)
                                                                                           tttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGt                                                   >  1:4224841/1‑150 (MQ=255)
                                                                                            ttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTc                                                  <  2:3219473/150‑1 (MQ=255)
                                                                                            ttACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTc                                                  >  2:2941093/1‑150 (MQ=255)
                                                                                             tACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGACCGTTTAATCATGCATTATTGGAAGATGCCAGCGACTGCAGCGATCTTCTTAAGCTATATAAAAATGTCg                                                 >  2:2805963/1‑150 (MQ=255)
                                                                                             tACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCg                                                 >  1:1787197/1‑150 (MQ=255)
                                                                                             tACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCg                                                 >  2:4319000/1‑150 (MQ=255)
                                                                                             tACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCg                                                 >  2:1605851/1‑150 (MQ=255)
                                                                                                gggTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTg                                              <  1:3912342/150‑1 (MQ=255)
                                                                                                gggTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTg                                              <  1:4519825/150‑1 (MQ=255)
                                                                                                    aaaCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTaaa                                          >  2:1108793/1‑150 (MQ=255)
                                                                                                    aaaCACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTaaa                                          <  2:1879838/150‑1 (MQ=255)
                                                                                                          ccTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAtgt                                    <  1:2201093/150‑1 (MQ=255)
                                                                                                           cTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAtgtg                                   <  1:790974/150‑1 (MQ=255)
                                                                                                             aaataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGtt                                 >  1:1525660/1‑150 (MQ=255)
                                                                                                               ataaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTa                               >  2:890400/1‑150 (MQ=255)
                                                                                                                taaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAg                              <  1:3918710/150‑1 (MQ=255)
                                                                                                                 aaaCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGc                             >  1:3443264/1‑150 (MQ=255)
                                                                                                                   aCTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCa                           >  1:4396822/1‑150 (MQ=255)
                                                                                                                     tGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGACATc                         >  1:2482534/1‑150 (MQ=255)
                                                                                                                      ggTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATcc                        >  2:5117551/1‑150 (MQ=255)
                                                                                                                      ggTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATcc                        >  1:4433691/1‑150 (MQ=255)
                                                                                                                      ggTACCCTACGCGGCACAACGATTTAGTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCCTGCATTATAGGACGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATTTCGCTGTAAAACCTGTGTTTAGACTTcc                        >  2:4401727/1‑150 (MQ=255)
                                                                                                                       gTACCCTACGCGTCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCa                       <  1:5093180/150‑1 (MQ=255)
                                                                                                                          cccTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGAt                    >  2:1673534/1‑150 (MQ=255)
                                                                                                                           ccTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATg                   >  1:2605847/1‑150 (MQ=255)
                                                                                                                           ccTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATg                   >  1:2426985/1‑150 (MQ=255)
                                                                                                                             tACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTc                 <  2:661294/150‑1 (MQ=255)
                                                                                                                             tACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTc                 <  1:1961660/150‑1 (MQ=255)
                                                                                                                                  ggCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCg            >  1:3069741/1‑150 (MQ=255)
                                                                                                                                  ggCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCg            >  1:3069686/1‑150 (MQ=255)
                                                                                                                                      caACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCtt        <  1:2786272/150‑1 (MQ=255)
                                                                                                                                      caACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCtt        <  2:5087641/150‑1 (MQ=255)
                                                                                                                                        ncGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGa      >  1:1028642/2‑150 (MQ=255)
                                                                                                                                         cGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGaa     <  2:3740204/150‑1 (MQ=255)
                                                                                                                                          gATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAAt    <  1:1561111/150‑1 (MQ=255)
                                                                                                                                           atttatttATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAAtt   >  2:1232995/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG  >  CP009273/176637‑176926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGGGC  >  CP009273/176634‑176932
                                                                                                                                                     |                                                                                                                                                     
AAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTT                                                                                                                                                       <  SRR19973063.2649812/150‑1 (MQ=60)
   GTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATA                                                                                                                                                    >  SRR19973063.1306362/1‑150 (MQ=60)
    TTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAA                                                                                                                                                   >  SRR19973063.3441250/1‑150 (MQ=60)
       GCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCT                                                                                                                                                <  SRR19973063.535773/150‑1 (MQ=60)
         TCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGC                                                                                                                                              >  SRR19973063.4715449/1‑150 (MQ=60)
          CTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCC                                                                                                                                             <  SRR19973063.3720054/150‑1 (MQ=60)
                 CTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATT                                                                                                                                      <  SRR19973063.4033827/150‑1 (MQ=60)
                    GTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTC                                                                                                                                   >  SRR19973063.2708740/1‑150 (MQ=60)
                       GTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCC                                                                                                                                <  SRR19973063.654111/150‑1 (MQ=60)
                           AATATGACTGGGAAAAATCACGCTCAAATAGTTTAAGCAGCAGTACGGAAGATCAGTTTTTTATTTATTTTCGGGTAAACACCCTTAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAA                                                                                                                            <  SRR19973063.4883658/150‑1 (MQ=60)
                            AAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAAC                                                                                                                           <  SRR19973063.2066153/150‑1 (MQ=60)
                             AATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACG                                                                                                                          >  SRR19973063.2057279/1‑150 (MQ=60)
                                GCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTT                                                                                                                       <  SRR19973063.3477226/150‑1 (MQ=60)
                                GCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTT                                                                                                                       <  SRR19973063.3477121/150‑1 (MQ=60)
                                   TGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAT                                                                                                                    <  SRR19973063.60050/150‑1 (MQ=60)
                                   TGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAAT                                                                                                                    >  SRR19973063.4589081/1‑150 (MQ=60)
                                        AAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGC                                                                                                               <  SRR19973063.571927/150‑1 (MQ=60)
                                          AATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCAT                                                                                                             <  SRR19973063.2755968/150‑1 (MQ=60)
                                          AATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCAT                                                                                                             >  SRR19973063.4458578/1‑150 (MQ=60)
                                           ATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATT                                                                                                            <  SRR19973063.3284274/150‑1 (MQ=60)
                                            TCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTA                                                                                                           <  SRR19973063.1200376/150‑1 (MQ=60)
                                             CACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTAT                                                                                                          >  SRR19973063.3883214/1‑150 (MQ=60)
                                             CACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTAT                                                                                                          <  SRR19973063.2432016/150‑1 (MQ=60)
                                             CACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTAT                                                                                                          >  SRR19973063.4379235/1‑150 (MQ=60)
                                             CACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTAT                                                                                                          >  SRR19973063.4379188/1‑150 (MQ=60)
                                               CGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTG                                                                                                        >  SRR19973063.1545202/1‑150 (MQ=60)
                                                   CAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAG                                                                                                    >  SRR19973063.4929524/1‑150 (MQ=60)
                                                        AGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCC                                                                                               <  SRR19973063.3758135/150‑1 (MQ=60)
                                                        AGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCC                                                                                               <  SRR19973063.3758022/150‑1 (MQ=60)
                                                        AGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCC                                                                                               <  SRR19973063.1039147/150‑1 (MQ=60)
                                                         GTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGTTAAAAACCCTAAATAAAATGGTACACTACGCGGCACAAAGATTTATTTATAATCTGCCTGCGATTTTCGCAGGAACGATTAATCATGCATTATTGGTAGATGCCA                                                                                              >  SRR19973063.907707/1‑150 (MQ=60)
                                                         GTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCA                                                                                              <  SRR19973063.4893910/150‑1 (MQ=60)
                                                          TTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAG                                                                                             <  SRR19973063.1982/150‑1 (MQ=60)
                                                             AAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGA                                                                                          >  SRR19973063.2716899/1‑150 (MQ=60)
                                                             AAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGA                                                                                          >  SRR19973063.2716942/1‑150 (MQ=60)
                                                              AGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCAGTATTGGAAGATGCCAGCGAA                                                                                         >  SRR19973063.422485/1‑150 (MQ=60)
                                                              AGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACGGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAC                                                                                         >  SRR19973063.3019126/1‑150 (MQ=60)
                                                                CCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATG                                                                                       <  SRR19973063.2005501/150‑1 (MQ=60)
                                                                  GCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCA                                                                                     <  SRR19973063.3750987/150‑1 (MQ=60)
                                                                  GCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCA                                                                                     <  SRR19973063.3750932/150‑1 (MQ=60)
                                                                  GCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCA                                                                                     >  SRR19973063.2932861/1‑150 (MQ=60)
                                                                   CAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAG                                                                                    >  SRR19973063.4248475/1‑150 (MQ=60)
                                                                   CAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCCGCACAACGATTTATTTATAATCTGCCTGCCATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATCCCAGCGAAAGCAG                                                                                    >  SRR19973063.4248566/1‑150 (MQ=60)
                                                                    AGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGC                                                                                   >  SRR19973063.3310865/1‑150 (MQ=60)
                                                                     GTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCG                                                                                  <  SRR19973063.4606852/150‑1 (MQ=60)
                                                                     GTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCG                                                                                  <  SRR19973063.4606816/150‑1 (MQ=60)
                                                                       ACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGAT                                                                                <  SRR19973063.4958716/150‑1 (MQ=60)
                                                                        CGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATC                                                                               >  SRR19973063.2957894/1‑150 (MQ=60)
                                                                         GGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCT                                                                              >  SRR19973063.633870/1‑150 (MQ=60)
                                                                           AAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTC                                                                            >  SRR19973063.3420204/1‑150 (MQ=60)
                                                                              ATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTA                                                                         <  SRR19973063.4341537/150‑1 (MQ=60)
                                                                                CAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAG                                                                       >  SRR19973063.2665782/1‑150 (MQ=60)
                                                                                CAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAG                                                                       >  SRR19973063.2665774/1‑150 (MQ=60)
                                                                                 AGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGC                                                                      >  SRR19973063.2087077/1‑150 (MQ=60)
                                                                                   TTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTA                                                                    <  SRR19973063.4552649/150‑1 (MQ=60)
                                                                                    TTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTAT                                                                   <  SRR19973063.203441/150‑1 (MQ=60)
                                                                                    TTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTAT                                                                   >  SRR19973063.3280793/1‑150 (MQ=60)
                                                                                    TTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTAT                                                                   <  SRR19973063.203446/150‑1 (MQ=60)
                                                                                    TTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTAT                                                                   >  SRR19973063.1286233/1‑150 (MQ=60)
                                                                                     TTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATA                                                                  <  SRR19973063.2741115/150‑1 (MQ=60)
                                                                                       TTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATA                                                                <  SRR19973063.1837572/150‑1 (MQ=60)
                                                                                        TATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAA                                                               >  SRR19973063.343550/1‑150 (MQ=60)
                                                                                              TTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGT                                                         >  SRR19973063.4238306/1‑150 (MQ=60)
                                                                                               TTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTC                                                        >  SRR19973063.2478613/1‑150 (MQ=60)
                                                                                                TACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCG                                                       >  SRR19973063.1792724/1‑150 (MQ=60)
                                                                                                   GGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTG                                                    <  SRR19973063.3924819/150‑1 (MQ=60)
                                                                                                   GGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTG                                                    >  SRR19973063.2647593/1‑150 (MQ=60)
                                                                                                   GGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTG                                                    <  SRR19973063.4534328/150‑1 (MQ=60)
                                                                                                    GGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGT                                                   >  SRR19973063.3441343/1‑150 (MQ=60)
                                                                                                       AAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAA                                                <  SRR19973063.3984915/150‑1 (MQ=60)
                                                                                                          CACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACA                                             >  SRR19973063.4628037/1‑150 (MQ=60)
                                                                                                           ACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACAT                                            >  SRR19973063.168871/1‑150 (MQ=60)
                                                                                                             CCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGT                                          <  SRR19973063.2208018/150‑1 (MQ=60)
                                                                                                              CTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTG                                         <  SRR19973063.793260/150‑1 (MQ=60)
                                                                                                              CTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTG                                         <  SRR19973063.3385460/150‑1 (MQ=60)
                                                                                                                AAATAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTT                                       >  SRR19973063.1530334/1‑150 (MQ=60)
                                                                                                                   TAAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAG                                    <  SRR19973063.3931215/150‑1 (MQ=60)
                                                                                                                    AAACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGC                                   >  SRR19973063.3454212/1‑150 (MQ=60)
                                                                                                                      ACTGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCA                                 >  SRR19973063.4410905/1‑150 (MQ=60)
                                                                                                                        TGGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGACATC                               >  SRR19973063.2490464/1‑150 (MQ=60)
                                                                                                                         GGTACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCC                              >  SRR19973063.4447921/1‑150 (MQ=60)
                                                                                                                          GTACCCTACGCGTCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCA                             <  SRR19973063.5109570/150‑1 (MQ=60)
                                                                                                                           TACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAG                            <  SRR19973063.3555138/150‑1 (MQ=60)
                                                                                                                           TACCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAG                            <  SRR19973063.3061600/150‑1 (MQ=60)
                                                                                                                             CCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGAT                          <  SRR19973063.2763730/150‑1 (MQ=60)
                                                                                                                             CCCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGAT                          >  SRR19973063.4431003/1‑150 (MQ=60)
                                                                                                                              CCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATG                         >  SRR19973063.2434743/1‑150 (MQ=60)
                                                                                                                              CCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATG                         >  SRR19973063.2614111/1‑150 (MQ=60)
                                                                                                                              CCTACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATG                         >  SRR19973063.2614127/1‑150 (MQ=60)
                                                                                                                                TACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTC                       <  SRR19973063.1967802/150‑1 (MQ=60)
                                                                                                                                TACGCGGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTC                       <  SRR19973063.891470/150‑1 (MQ=60)
                                                                                                                                     GGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCG                  >  SRR19973063.3079544/1‑150 (MQ=60)
                                                                                                                                     GGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCG                  >  SRR19973063.3079599/1‑150 (MQ=60)
                                                                                                                                     GGCACAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCG                  <  SRR19973063.3104588/150‑1 (MQ=60)
                                                                                                                                         CAACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTT              <  SRR19973063.2795244/150‑1 (MQ=60)
                                                                                                                                          AACGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTG             <  SRR19973063.2842975/150‑1 (MQ=60)
                                                                                                                                           NCGATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGA            >  SRR19973063.1031742/1‑150 (MQ=60)
                                                                                                                                             GATTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAAT          <  SRR19973063.1565901/150‑1 (MQ=60)
                                                                                                                                               TTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG        <  SRR19973063.2325614/150‑1 (MQ=60)
                                                                                                                                               TTTATTTATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTG        <  SRR19973063.970939/150‑1 (MQ=60)
                                                                                                                                                     TATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGGGC  <  SRR19973063.4719159/150‑1 (MQ=60)
                                                                                                                                                     TATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGGGC  <  SRR19973063.4719147/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGCTCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACCCTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGGGC  >  CP009273/176634‑176932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑