Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F224 I0 R1	1457	284.0	9290224	95.7%	8890744	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	652,865	Δ1 bp	91.5%	coding (118/210 nt)	cspE →	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	652,865	0	G	.	91.5%	1224.8 / 111.8	272	coding (118/210 nt)	cspE	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand): ref base G (12/11); new base . (130/119); total (142/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 278 total aligned reads displayed.

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTT  >  CP009273/652716‑653012

ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                                                     <  2:2731752/150‑1 (MQ=255)

 tgtgTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATgt                                                                                                                                                    <  2:1304988/150‑2 (MQ=255)

   tgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGttt                                                                                                                                                  <  2:3053442/150‑1 (MQ=255)

   tgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGttt                                                                                                                                                  <  2:3811108/150‑1 (MQ=255)

   tgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGttt                                                                                                                                                  >  2:4134272/1‑150 (MQ=255)

    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                                 <  1:4442367/150‑1 (MQ=255)

    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                                 >  2:373214/1‑150 (MQ=255)

         tttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCATACTAAT‑GTTTTAAAAc                                                                                                                                           <  2:857720/150‑1 (MQ=255)

          ttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAct                                                                                                                                          >  2:1917785/1‑150 (MQ=255)

          ttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAct                                                                                                                                          >  2:532086/1‑150 (MQ=255)

              cATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTg                                                                                                                                      >  2:3022093/1‑150 (MQ=255)

                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                    <  2:2423840/150‑1 (MQ=255)

                 gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTg                                                                                                                                   >  1:1268784/1‑150 (MQ=255)

                  tAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTg                                                                                                                                   >  1:2227652/1‑150 (MQ=255)

                  tAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGa                                                                                                                                  >  2:4336741/1‑150 (MQ=255)

                     aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAgg                                                                                                                               <  2:3107583/150‑1 (MQ=255)

                      ggTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGt                                                                                                                              >  1:2326259/1‑150 (MQ=255)

                      ggTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGt                                                                                                                              <  1:4569287/150‑1 (MQ=255)

                       gTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTc                                                                                                                             <  2:2501073/150‑1 (MQ=255)

                        tAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCa                                                                                                                            <  1:4108963/150‑1 (MQ=255)

                        tAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCa                                                                                                                            <  2:4216332/150‑1 (MQ=255)

                           ttttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAgcg                                                                                                                         <  2:1692850/150‑1 (MQ=255)

                            tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAgcgc                                                                                                                        <  2:1098914/150‑1 (MQ=255)

                             ttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGcgcg                                                                                                                       <  2:2052378/150‑1 (MQ=255)

                              tGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGt                                                                                                                      >  2:2293903/1‑150 (MQ=255)

                               gATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTa                                                                                                                     >  1:1720608/1‑150 (MQ=255)

                                aTGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTag                                                                                                                    >  1:3442699/1‑150 (MQ=255)

                                   tCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGt                                                                                                                 >  2:157066/1‑150 (MQ=255)

                                     tAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTc                                                                                                               <  1:2430117/150‑1 (MQ=255)

                                      aaGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCg                                                                                                              <  2:1800266/150‑1 (MQ=255)

                                        gATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGaa                                                                                                            >  1:1829582/1‑150 (MQ=255)

                                          ttAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAt                                                                                                          <  1:4107813/150‑1 (MQ=255)

                                           tAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                                                         >  2:2090496/1‑150 (MQ=255)

                                           tAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                                                         <  1:3334503/150‑1 (MQ=255)

                                           tAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                                                         <  2:2746427/150‑1 (MQ=255)

                                              aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                      <  1:727294/150‑1 (MQ=255)

                                              aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                      <  1:2540303/150‑1 (MQ=255)

                                              aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                      <  1:1548055/150‑1 (MQ=255)

                                               ggTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                      <  1:3700808/150‑1 (MQ=255)

                                                 tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                                                   >  1:4215706/1‑150 (MQ=255)

                                                 tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                                                   >  1:3096300/1‑150 (MQ=255)

                                                     gTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTg                                                                                               <  2:1370882/150‑1 (MQ=255)

                                                     gTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTg                                                                                               >  2:1596076/1‑150 (MQ=255)

                                                      ttAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGc                                                                                              <  2:3203126/150‑1 (MQ=255)

                                                       tAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGcc                                                                                             >  2:909568/1‑150 (MQ=255)

                                                             gTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGc                                                                                       <  1:2010991/150‑1 (MQ=255)

                                                               ttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGccc                                                                                     <  1:2738393/150‑1 (MQ=255)

                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                    >  1:3853149/1‑150 (MQ=255)

                                                                 aaTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCtt                                                                                   <  1:2111017/150‑1 (MQ=255)

                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                  <  2:29502/150‑1 (MQ=255)

                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAACACTAACGGTGCCAAAGGCCCTTc                                                                                  >  2:3629177/1‑150 (MQ=255)

                                                                    gAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctg                                                                                >  1:1246793/1‑150 (MQ=255)

                                                                    gAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctg                                                                                >  1:1440783/1‑150 (MQ=255)

                                                                      gTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgct                                                                              >  2:4607293/1‑150 (MQ=255)

                                                                           aaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaaa                                                                         >  2:765074/1‑150 (MQ=255)

                                                                             ggATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACg                                                                       <  2:72622/150‑1 (MQ=255)

                                                                              gATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGt                                                                      >  1:1546259/1‑150 (MQ=255)

                                                                              gATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGATCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGt                                                                      >  2:2797596/1‑150 (MQ=255)

                                                                               aTTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCACACgga                                                                     >  2:2210007/1‑148 (MQ=255)

                                                                                ttCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTaa                                                                    >  2:3349708/1‑150 (MQ=255)

                                                                                  cGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATc                                                                  >  2:148498/1‑150 (MQ=255)

                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                                 <  1:373214/150‑1 (MQ=255)

                                                                                     tttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGct                                                               <  2:648414/150‑1 (MQ=255)

                                                                                     tttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGct                                                               <  1:3517342/150‑1 (MQ=255)

                                                                                        cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                                                            >  2:151630/1‑150 (MQ=255)

                                                                                        cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                                                            <  1:4326160/150‑1 (MQ=255)

                                                                                        cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                                                            >  1:1007704/1‑150 (MQ=255)

                                                                                          ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTa                                                          >  1:2827271/1‑150 (MQ=255)

                                                                                          ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTa                                                          >  1:3211709/1‑150 (MQ=255)

                                                                                           tACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTaa                                                         <  1:758291/150‑1 (MQ=255)

                                                                                            aCTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAg                                                        >  1:2114841/1‑150 (MQ=255)

                                                                                             cTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGa                                                       <  1:992778/150‑1 (MQ=255)

                                                                                              tCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGAt                                                      <  1:4413041/150‑1 (MQ=255)

                                                                                                cGGAAGACGTCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTATAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc                                                    <  2:4006210/150‑1 (MQ=255)

                                                                                                        ggCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCaa                                            >  1:500299/1‑150 (MQ=255)

                                                                                                          cagcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGa                                          >  2:3110461/1‑150 (MQ=255)

                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                         >  2:4295656/1‑150 (MQ=255)

                                                                                                             caAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                       <  1:4020357/150‑1 (MQ=255)

                                                                                                             caAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                       <  1:1701920/150‑1 (MQ=255)

                                                                                                                aGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaa                                    <  2:185300/150‑1 (MQ=255)

                                                                                                                  aCGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaaaa                                  >  1:2630434/1‑150 (MQ=255)

                                                                                                                   cGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAc                                 >  1:194667/1‑150 (MQ=255)

                                                                                                                   cGTGTTCGTACACGTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAc                                 <  1:855474/150‑1 (MQ=255)

                                                                                                                        tCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCt                            <  1:3381686/150‑1 (MQ=255)

                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                           >  2:1982387/1‑150 (MQ=255)

                                                                                                                          gTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTa                          <  1:2781158/150‑1 (MQ=255)

                                                                                                                           tACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTaa                         >  1:3172473/1‑150 (MQ=255)

                                                                                                                              acTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                      <  1:3955801/150‑1 (MQ=255)

                                                                                                                                 tctcTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                   <  1:2912368/150‑1 (MQ=255)

                                                                                                                                  ctctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg                  >  2:3301832/1‑150 (MQ=255)

                                                                                                                                  ctctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg                  >  1:2978470/1‑150 (MQ=255)

                                                                                                                                  ctctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg                  >  2:1283401/1‑150 (MQ=255)

                                                                                                                                   tctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                 <  1:4270743/150‑1 (MQ=255)

                                                                                                                                     tGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt               <  1:1050079/150‑1 (MQ=255)

                                                                                                                                     tGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt               <  1:3940369/150‑1 (MQ=255)

                                                                                                                                      gCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttt              >  2:3890600/1‑150 (MQ=255)

                                                                                                                                      gCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttt              >  2:3890520/1‑150 (MQ=255)

                                                                                                                                      gCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttt              <  1:3022367/150‑1 (MQ=255)

                                                                                                                                             aGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTgg       >  1:2476374/1‑150 (MQ=255)

                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt    >  1:1140205/1‑150 (MQ=255)

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTT  >  CP009273/652716‑653012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A