Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,865 Δ1 bp 86.9% coding (118/210 nt) cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,8650G.86.9% 796.2 / 129.8 199coding (118/210 nt)cspEconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (19/7);  new base . (83/90);  total (102/97)
Fisher's exact test for biased strand distribution p-value = 2.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 202 total aligned reads displayed.

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010
                                                                                                                                                     |                                                                                                                                                 
ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                                                   <  1:4168586/150‑1 (MQ=255)
ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                                                   <  1:1706158/150‑1 (MQ=255)
    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                               <  1:3626185/150‑1 (MQ=255)
      cTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaa                                                                                                                                            <  1:1032321/150‑1 (MQ=255)
        aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTaaa                                                                                                                                           >  1:2049236/1‑150 (MQ=255)
        aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaaaa                                                                                                                                          <  1:3871425/150‑1 (MQ=255)
             tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCtt                                                                                                                                     <  1:2392943/150‑1 (MQ=255)
             tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCtt                                                                                                                                     <  1:874084/150‑1 (MQ=255)
               aTGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGc                                                                                                                                   >  1:2032756/1‑150 (MQ=255)
               aTGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAATCTTGc                                                                                                                                   >  2:3567098/1‑150 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  <  1:3877874/150‑1 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCGGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  <  1:1699617/150‑1 (MQ=255)
                 gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTg                                                                                                                                 <  2:1030401/150‑1 (MQ=255)
                 gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTg                                                                                                                                 <  2:3433606/150‑1 (MQ=255)
                    aaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAg                                                                                                                              >  2:2607220/1‑150 (MQ=255)
                     aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAgg                                                                                                                             <  2:3070070/150‑1 (MQ=255)
                     aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAgg                                                                                                                             >  1:1852870/1‑150 (MQ=255)
                       gTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTc                                                                                                                           <  2:4538729/150‑1 (MQ=255)
                         aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCa                                                                                                                          <  2:1488801/150‑1 (MQ=255)
                         aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAg                                                                                                                         >  1:2261068/1‑150 (MQ=255)
                          aTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAg                                                                                                                         >  1:2126790/1‑150 (MQ=255)
                          aTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAgc                                                                                                                        >  2:2088517/1‑150 (MQ=255)
                           ttttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAgcg                                                                                                                       <  2:1036893/150‑1 (MQ=255)
                                 tGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTaga                                                                                                                 <  1:3265239/150‑1 (MQ=255)
                                  gTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTaga                                                                                                                 >  2:2074421/1‑150 (MQ=255)
                                   tCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGt                                                                                                               <  1:437616/150‑1 (MQ=255)
                                    cTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGtt                                                                                                              >  1:1261314/1‑150 (MQ=255)
                                     tAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTc                                                                                                             >  2:3298415/1‑150 (MQ=255)
                                       aGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGa                                                                                                           >  2:4216439/1‑150 (MQ=255)
                                        gATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGaa                                                                                                          <  2:202614/150‑1 (MQ=255)
                                         aTTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGaaa                                                                                                         >  1:2442713/1‑150 (MQ=255)
                                            aaaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCa                                                                                                      <  1:2304241/150‑1 (MQ=255)
                                             aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTCAT‑GTTTTAAAACTCGTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCAc                                                                                                     >  2:697604/1‑150 (MQ=255)
                                             aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCAc                                                                                                     >  2:1572029/1‑150 (MQ=255)
                                             aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCAc                                                                                                     >  2:3366052/1‑150 (MQ=255)
                                                   aCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACgg                                                                                               >  2:3823644/1‑150 (MQ=255)
                                                   aCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACgg                                                                                               <  2:4488436/150‑1 (MQ=255)
                                                     gTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTg                                                                                             <  1:4365976/150‑1 (MQ=255)
                                                       tAAGTGGTTTAATGAGTCCAATGGATTCGGTTTCATTACTCCGGAATTCGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGATGGTCAGCGCGTAGAGTTCGAAGTCACTAACGGTGc                                                                                            >  2:4003728/1‑150 (MQ=255)
                                                         aGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCa                                                                                          >  2:4288238/1‑150 (MQ=255)
                                                          gTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTCACGGTGCCaaa                                                                                        >  2:1896357/1‑150 (MQ=255)
                                                           tGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAg                                                                                       <  2:1643666/150‑1 (MQ=255)
                                                            ggTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAgg                                                                                      >  1:1004318/1‑150 (MQ=255)
                                                             gTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGc                                                                                     <  2:2757778/150‑1 (MQ=255)
                                                              tttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGc                                                                                     >  2:3804371/1‑150 (MQ=255)
                                                               ttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGccc                                                                                   >  2:861279/1‑150 (MQ=255)
                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGccca                                                                                  <  2:1003630/150‑2 (MQ=255)
                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGTCCCTTc                                                                                >  1:1981245/1‑150 (MQ=255)
                                                                   tGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                >  1:3069472/1‑150 (MQ=255)
                                                                     aGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgc                                                                             >  1:2140223/1‑150 (MQ=255)
                                                                       tCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACTGTGCCAAAGGCCCTTctgctg                                                                           >  1:2125483/1‑150 (MQ=255)
                                                                       tCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgctg                                                                           <  2:2254740/150‑1 (MQ=255)
                                                                       tCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgctg                                                                           <  2:2254636/150‑1 (MQ=255)
                                                                            aGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCacac                                                                      >  2:3512771/1‑150 (MQ=255)
                                                                              gATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACg                                                                     <  2:43452/150‑1 (MQ=255)
                                                                              gATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGt                                                                    >  2:4395601/1‑150 (MQ=255)
                                                                               aTTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTa                                                                   >  1:2328492/1‑150 (MQ=255)
                                                                                 tCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAAt                                                                 <  1:1015065/150‑1 (MQ=255)
                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                               >  2:4190105/1‑150 (MQ=255)
                                                                                     tttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGct                                                             >  1:2895591/1‑150 (MQ=255)
                                                                                      ttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGctc                                                            >  2:3951428/1‑150 (MQ=255)
                                                                                       tCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGctct                                                           <  1:2557295/150‑1 (MQ=255)
                                                                                       tCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGctct                                                           <  2:3607818/150‑1 (MQ=255)
                                                                                        cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                                                          >  2:122848/1‑150 (MQ=255)
                                                                                          ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTa                                                        <  1:4209772/150‑1 (MQ=255)
                                                                                                cGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc                                                  <  2:815776/150‑1 (MQ=255)
                                                                                                 ggAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACg                                                 >  2:1048502/1‑150 (MQ=255)
                                                                                                 ggAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACg                                                 <  1:2901956/150‑1 (MQ=255)
                                                                                                     gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg                                             >  2:673311/1‑150 (MQ=255)
                                                                                                         gcagcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAg                                         <  2:200943/150‑1 (MQ=255)
                                                                                                           agcaAAGACGTGTTCTTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       <  1:1830514/150‑1 (MQ=255)
                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       >  1:3760577/1‑150 (MQ=255)
                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       <  2:4221925/150‑1 (MQ=255)
                                                                                                             caAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                     <  1:1373328/150‑1 (MQ=255)
                                                                                                              aaaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                     >  2:3487928/1‑150 (MQ=255)
                                                                                                               aaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCa                                   >  1:4457001/1‑150 (MQ=255)
                                                                                                               aaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCa                                   >  2:841549/1‑150 (MQ=255)
                                                                                                                aGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaa                                  >  2:4503125/1‑150 (MQ=255)
                                                                                                                   cGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAc                               >  1:1327017/1‑150 (MQ=255)
                                                                                                                     tgtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAccc                             <  2:704133/150‑1 (MQ=255)
                                                                                                                     tgtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAccc                             <  2:704259/150‑1 (MQ=255)
                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                         >  1:1525630/1‑150 (MQ=255)
                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                         >  1:2246692/1‑150 (MQ=255)
                                                                                                                           tACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTaa                       <  1:1217198/150‑1 (MQ=255)
                                                                                                                           tACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTaa                       >  2:2704887/1‑150 (MQ=255)
                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     >  2:2963569/1‑150 (MQ=255)
                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     <  2:1472037/150‑1 (MQ=255)
                                                                                                                              acTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                    >  1:3631901/1‑150 (MQ=255)
                                                                                                                               cTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                    >  1:2743190/1‑150 (MQ=255)
                                                                                                                                       cAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt           <  1:488639/150‑1 (MQ=255)
                                                                                                                                       cAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAAGCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt           >  2:3929668/1‑150 (MQ=255)
                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          <  2:2891486/150‑1 (MQ=255)
                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          <  2:1327017/150‑1 (MQ=255)
                                                                                                                                           ccAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt       >  2:1088461/1‑150 (MQ=255)
                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      <  1:859351/150‑1 (MQ=255)
                                                                                                                                              gACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCACGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt    >  2:2487876/1‑150 (MQ=255)
                                                                                                                                                cTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc   <  2:582398/150‑1 (MQ=255)
                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTcc  >  2:3216315/1‑149 (MQ=255)
                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt  >  1:2187590/1‑150 (MQ=255)
                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt  >  1:4108758/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A