Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,865 Δ1 bp 94.2% coding (118/210 nt) cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,8650G.94.2% 1080.5 / 57.2 225coding (118/210 nt)cspEconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (9/4);  new base . (102/110);  total (111/114)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010
                                                                                                                                                     |                                                                                                                                                 
ttGTGTCTATTTTTCATGTAAAGGTAATTGTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                                                   >  2:3876214/1‑150 (MQ=255)
 tgtgTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGAAGTGTTCGTACACTTCTCTGCAATCCATACTAATgt                                                                                                                                                  >  2:2484921/1‑149 (MQ=255)
  gtgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATgtt                                                                                                                                                 >  2:2587756/1‑148 (MQ=255)
    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTCACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGttt                                                                                                                                               >  2:2876971/1‑150 (MQ=255)
        aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaaaa                                                                                                                                          <  2:474377/150‑1 (MQ=255)
        aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGAATCGGTTTCATTACTCCGGAATACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaaaa                                                                                                                                          >  2:2067276/1‑150 (MQ=255)
         tttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAc                                                                                                                                         <  2:1370823/150‑1 (MQ=255)
          ttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAct                                                                                                                                        >  1:2007474/1‑150 (MQ=255)
             tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCtt                                                                                                                                     >  1:25282/1‑150 (MQ=255)
              cATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTg                                                                                                                                    <  1:457478/150‑1 (MQ=255)
              cATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTg                                                                                                                                    <  1:457403/150‑1 (MQ=255)
               aTGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGc                                                                                                                                   >  1:2414355/1‑150 (MQ=255)
               aTGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGAAGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGc                                                                                                                                   >  1:2125183/1‑150 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  >  1:1777192/1‑150 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  >  1:2381435/1‑150 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  <  1:3256135/150‑1 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  >  2:1056841/1‑150 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  >  1:843942/1‑150 (MQ=255)
                 gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTg                                                                                                                                 <  1:1563828/150‑1 (MQ=255)
                 gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTg                                                                                                                                 <  1:1563598/150‑1 (MQ=255)
                    aaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAg                                                                                                                              <  1:1116642/150‑1 (MQ=255)
                     aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAgg                                                                                                                             <  2:4132939/150‑1 (MQ=255)
                       gTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTc                                                                                                                           >  2:2238261/1‑150 (MQ=255)
                               gATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTa                                                                                                                   <  2:2918532/150‑1 (MQ=255)
                               gATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTa                                                                                                                   <  1:218222/150‑1 (MQ=255)
                                 tGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTaga                                                                                                                 <  2:2168740/150‑1 (MQ=255)
                                   tCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGt                                                                                                               <  2:1576932/150‑1 (MQ=255)
                                    cTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGtt                                                                                                              >  2:3796194/1‑150 (MQ=255)
                                    cTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGtt                                                                                                              <  2:1304690/150‑1 (MQ=255)
                                      aaGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCg                                                                                                            <  2:1020997/150‑1 (MQ=255)
                                       aGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGa                                                                                                           >  2:1048427/1‑150 (MQ=255)
                                       aGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGa                                                                                                           <  1:18610/150‑1 (MQ=255)
                                       aGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGa                                                                                                           >  2:4088117/1‑150 (MQ=255)
                                          ttAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAt                                                                                                        <  1:2954367/150‑1 (MQ=255)
                                          ttAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAt                                                                                                        >  1:1702057/1‑150 (MQ=255)
                                          ttAAAGGTAACATTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAt                                                                                                        <  1:3438485/150‑1 (MQ=255)
                                           tAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                                                       <  2:2784664/150‑1 (MQ=255)
                                                 tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                                                 >  2:3485967/1‑150 (MQ=255)
                                                   aCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACg                                                                                                <  2:4051623/150‑1 (MQ=255)
                                                     gTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTg                                                                                             <  1:4038576/150‑1 (MQ=255)
                                                     gTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTg                                                                                             >  1:1978867/1‑150 (MQ=255)
                                                         aGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGTTCAGCGCGTAGAGTTAGAAATCACTAACGGTGACaa                                                                                         >  2:2945609/1‑150 (MQ=255)
                                                          gTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCaaa                                                                                        >  2:132728/1‑150 (MQ=255)
                                                          gTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCaaa                                                                                        >  1:1858743/1‑150 (MQ=255)
                                                               ttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGccc                                                                                   <  1:4132627/150‑1 (MQ=255)
                                                               ttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGccc                                                                                   >  1:3511660/1‑150 (MQ=255)
                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  <  1:3854720/150‑1 (MQ=255)
                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  >  2:1773090/1‑150 (MQ=255)
                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  <  2:640856/150‑1 (MQ=255)
                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  <  2:2495801/150‑1 (MQ=255)
                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  >  2:3638419/1‑150 (MQ=255)
                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                <  1:927339/150‑1 (MQ=255)
                                                                   tGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTct                                                                               <  1:2621370/150‑1 (MQ=255)
                                                                   tGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTct                                                                               >  1:1698174/1‑150 (MQ=255)
                                                                   tGAGTCCAAAGGATGCGGTTTCATTACTCCGGAAGCCGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                >  1:4424415/1‑150 (MQ=255)
                                                                    gAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctg                                                                              <  1:1648431/150‑1 (MQ=255)
                                                                     aGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgc                                                                             >  1:3197410/1‑150 (MQ=255)
                                                                      gTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgct                                                                            >  2:702091/1‑150 (MQ=255)
                                                                          aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaa                                                                        >  2:1719781/1‑150 (MQ=255)
                                                                          aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaa                                                                        >  1:1313621/1‑150 (MQ=255)
                                                                          aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaa                                                                        >  1:3586731/1‑150 (MQ=255)
                                                                               aTTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAATCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACTGTGACAAAGTCCCTTCTGCTGCAAACGTa                                                                   >  2:1611069/1‑150 (MQ=255)
                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                               <  1:1888121/150‑1 (MQ=255)
                                                                                       tCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGctct                                                           <  1:1948137/150‑1 (MQ=255)
                                                                                       tCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGctct                                                           <  1:1947084/150‑1 (MQ=255)
                                                                                        cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                                                          >  2:222095/1‑150 (MQ=255)
                                                                                          ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTa                                                        >  2:22474/1‑150 (MQ=255)
                                                                                           tACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTaa                                                       <  1:2071193/150‑1 (MQ=255)
                                                                                                cGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc                                                  <  1:1188623/150‑1 (MQ=255)
                                                                                                 ggAAGACGGCATCAAAGACGTGTTCGTACACTTCTCTTCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACg                                                 <  1:56426/150‑1 (MQ=255)
                                                                                                  gAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGt                                                >  2:597070/1‑150 (MQ=255)
                                                                                                  gAAGACGGCAGCAAAGAAGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGt                                                <  2:2125183/150‑1 (MQ=255)
                                                                                                    aGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCa                                              <  2:1755487/150‑1 (MQ=255)
                                                                                                     gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg                                             <  1:2304939/150‑1 (MQ=255)
                                                                                                      aCGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGc                                            <  2:1898854/150‑1 (MQ=255)
                                                                                                        ggCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCaa                                          <  2:4370404/150‑1 (MQ=255)
                                                                                                          cagcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGa                                        >  2:4340069/1‑150 (MQ=255)
                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       <  1:3271587/150‑1 (MQ=255)
                                                                                                                  aCGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaaaa                                <  2:253555/150‑1 (MQ=255)
                                                                                                                      gtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCg                            <  1:4123862/150‑1 (MQ=255)
                                                                                                                       ttCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGc                           <  2:2685884/150‑1 (MQ=255)
                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                         <  1:2195121/150‑1 (MQ=255)
                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAAATACGTCAGCAAGAATTCAAAACCCGCtt                         >  1:2802822/1‑150 (MQ=255)
                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     >  1:3200283/1‑150 (MQ=255)
                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     <  2:2270732/150‑1 (MQ=255)
                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     <  2:2270576/150‑1 (MQ=255)
                                                                                                                               cTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                   <  1:2537044/150‑1 (MQ=255)
                                                                                                                                ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                   <  1:529957/150‑1 (MQ=255)
                                                                                                                                ttCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc                  <  1:419123/150‑1 (MQ=255)
                                                                                                                                   tctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg               <  1:1736182/150‑1 (MQ=255)
                                                                                                                                   tctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg               <  1:3991594/150‑1 (MQ=255)
                                                                                                                                       cAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt           >  2:1233847/1‑150 (MQ=255)
                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          <  2:2476974/150‑1 (MQ=255)
                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          <  2:4522230/150‑1 (MQ=255)
                                                                                                                                          tCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttttt        <  1:1056841/150‑1 (MQ=255)
                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAACGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      <  2:777897/150‑1 (MQ=255)
                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      >  2:3063205/1‑150 (MQ=255)
                                                                                                                                             aGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTgg     >  1:3652744/1‑150 (MQ=255)
                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTCATCAGCGGGTTTTTTTTGGTCt  >  2:511299/1‑150 (MQ=255)
                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt  <  2:3367231/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A