Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	652,865	Δ1 bp	89.3%	coding (118/210 nt)	cspE →	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	652,865	0	G	.	89.3%	873.7 / 108.7	206	coding (118/210 nt)	cspE	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand): ref base G (15/7); new base . (104/80); total (119/87)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 212 total aligned reads displayed.

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010

ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                                                   <  1:3027209/150‑1 (MQ=255)

 tgtgTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATgt                                                                                                                                                  >  1:3952422/1‑149 (MQ=255)

  gtgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATgtt                                                                                                                                                 >  1:410656/1‑148 (MQ=255)

  gtgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATgtt                                                                                                                                                 >  1:2585430/1‑148 (MQ=255)

  gtgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCAGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATgtt                                                                                                                                                 >  1:2585502/1‑148 (MQ=255)

   tgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGttt                                                                                                                                                >  1:579372/1‑150 (MQ=255)

   tgtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGttt                                                                                                                                                <  1:2429240/150‑1 (MQ=255)

    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                               >  1:3885516/1‑150 (MQ=255)

    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                               >  2:329563/1‑150 (MQ=255)

    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                               >  2:2846704/1‑150 (MQ=255)

    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttt                                                                                                                                               >  1:1534072/1‑150 (MQ=255)

     tCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGtttta                                                                                                                                              >  2:1516583/1‑149 (MQ=255)

      cTATTTTTCATGTAAAGGTCATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaa                                                                                                                                            >  2:2763957/1‑150 (MQ=255)

        aTTTTTCATGTAAAGTTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaaaa                                                                                                                                          <  2:940749/150‑1 (MQ=255)

        aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaaaa                                                                                                                                          >  1:3120120/1‑150 (MQ=255)

         tttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAc                                                                                                                                         >  2:849261/1‑150 (MQ=255)

          ttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAct                                                                                                                                        >  1:1240371/1‑150 (MQ=255)

              cATGTAAAGGTATTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTg                                                                                                                                    <  1:1016668/150‑1 (MQ=255)

              cATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTg                                                                                                                                    <  2:250849/150‑1 (MQ=255)

               aTGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGc                                                                                                                                   >  1:1851965/1‑150 (MQ=255)

               aTGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGc                                                                                                                                   >  1:829045/1‑150 (MQ=255)

                 gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTg                                                                                                                                 <  2:2811367/150‑1 (MQ=255)

                   aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                                                                                                >  2:3173513/1‑150 (MQ=255)

                   aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAAAGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAATACGAGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGaa                                                                                                                               >  2:3613884/1‑150 (MQ=255)

                    aaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAg                                                                                                                              <  1:4356548/150‑1 (MQ=255)

                      ggTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGt                                                                                                                            <  1:2667011/150‑1 (MQ=255)

                         aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAg                                                                                                                         >  2:1332060/1‑150 (MQ=255)

                           ttttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgc                                                                                                                        <  2:684038/150‑1 (MQ=255)

                             ttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGcgcg                                                                                                                     >  1:2250881/1‑150 (MQ=255)

                              tGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGt                                                                                                                    >  2:2446090/1‑150 (MQ=255)

                                aTGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTag                                                                                                                  >  2:3382426/1‑150 (MQ=255)

                                     tAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTc                                                                                                             >  2:4110637/1‑150 (MQ=255)

                                      aaGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCg                                                                                                            <  2:3978205/150‑1 (MQ=255)

                                              aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                    >  1:99840/1‑150 (MQ=255)

                                              aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                    >  2:2469427/1‑150 (MQ=255)

                                                                tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  >  1:3498812/1‑150 (MQ=255)

                                                                 aaTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                                                  >  1:2845367/1‑150 (MQ=255)

                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                <  2:4100355/150‑1 (MQ=255)

                                                                        ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCtgctgc                                                                          >  1:634941/1‑150 (MQ=255)

                                                                        ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCtgctgc                                                                          <  2:104868/150‑1 (MQ=255)

                                                                        ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCtgctgc                                                                          <  2:2674954/150‑1 (MQ=255)

                                                                        ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCtgctgc                                                                          <  2:103978/150‑1 (MQ=255)

                                                                         cAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCa                                                                         >  1:3119644/1‑150 (MQ=255)

                                                                          aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaa                                                                        >  1:1371727/1‑150 (MQ=255)

                                                                             ggATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACg                                                                     <  1:3631163/150‑1 (MQ=255)

                                                                             ggATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCACTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTTAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACg                                                                     >  2:1625838/1‑150 (MQ=255)

                                                                              gATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGt                                                                    >  1:2133210/1‑150 (MQ=255)

                                                                                ttCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTac                                                                  >  2:1703701/1‑149 (MQ=255)

                                                                                ttCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTac                                                                  >  2:3892319/1‑149 (MQ=255)

                                                                                  cGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATc                                                                >  1:88568/1‑150 (MQ=255)

                                                                                  cGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATc                                                                >  2:171503/1‑150 (MQ=255)

                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                               <  2:4059391/150‑1 (MQ=255)

                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACAGGGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                               <  1:804498/150‑1 (MQ=255)

                                                                                        cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                                                          <  1:4169442/150‑1 (MQ=255)

                                                                                             cTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGa                                                     >  2:3452422/1‑150 (MQ=255)

                                                                                                cGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc                                                  <  1:2634087/150‑1 (MQ=255)

                                                                                                 ggAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACg                                                 >  1:3841815/1‑150 (MQ=255)

                                                                                                 ggAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACg                                                 <  2:1451055/150‑1 (MQ=255)

                                                                                                  gAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACg                                                 <  1:3557506/150‑1 (MQ=255)

                                                                                                   aaGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTc                                               >  2:4253530/1‑150 (MQ=255)

                                                                                                   aaGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTc                                               >  2:2085171/1‑150 (MQ=255)

                                                                                                     gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg                                             >  1:1500083/1‑150 (MQ=255)

                                                                                                      aCGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGc                                            >  1:1075926/1‑150 (MQ=255)

                                                                                                       cGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCa                                           <  2:3541439/150‑1 (MQ=255)

                                                                                                       cGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCa                                           >  1:61559/1‑150 (MQ=255)

                                                                                                             caAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                     <  1:2433639/150‑1 (MQ=255)

                                                                                                              aaaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                     <  1:2891992/150‑1 (MQ=255)

                                                                                                               aaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACATAATCGCTCTGTAAGATACGTCAGCAAGAATTCa                                   <  1:2631990/150‑1 (MQ=255)

                                                                                                                aGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaa                                  >  1:1276773/1‑150 (MQ=255)

                                                                                                                    gtgtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAcc                              >  1:3194770/1‑150 (MQ=255)

                                                                                                                     tgtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAccc                             >  1:95607/1‑150 (MQ=255)

                                                                                                                        tCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCt                          <  2:3839764/150‑1 (MQ=255)

                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                         <  1:329563/150‑1 (MQ=255)

                                                                                                                          gTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTa                        <  1:636201/150‑1 (MQ=255)

                                                                                                                          gTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTa                        >  1:3524483/1‑150 (MQ=255)

                                                                                                                            acacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                      <  1:826784/150‑1 (MQ=255)

                                                                                                                            acacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                      <  1:1750952/150‑1 (MQ=255)

                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     <  1:2624362/150‑1 (MQ=255)

                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     >  1:1687729/1‑150 (MQ=255)

                                                                                                                              acTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                    <  1:686197/150‑1 (MQ=255)

                                                                                                                              acTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                    <  2:33549/150‑1 (MQ=255)

                                                                                                                              acTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                    <  2:587834/150‑1 (MQ=255)

                                                                                                                               cTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                   <  2:2292033/150‑1 (MQ=255)

                                                                                                                               cTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                   <  2:1499376/150‑1 (MQ=255)

                                                                                                                                ttCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc                  >  2:2956622/1‑150 (MQ=255)

                                                                                                                                 tctcTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                 <  1:3488421/150‑1 (MQ=255)

                                                                                                                                  ctctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                 >  2:1865167/1‑150 (MQ=255)

                                                                                                                                  ctctGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg                <  1:1092288/150‑1 (MQ=255)

                                                                                                                                     tGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt             >  2:3003519/1‑150 (MQ=255)

                                                                                                                                     tGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt             >  2:701723/1‑150 (MQ=255)

                                                                                                                                       cAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt           >  1:1100646/1‑150 (MQ=255)

                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          >  2:520009/1‑150 (MQ=255)

                                                                                                                                           ccAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt       <  2:4051532/150‑1 (MQ=255)

                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAATATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      >  2:821179/1‑150 (MQ=255)

                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      >  2:1170963/1‑150 (MQ=255)

                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      <  2:3841828/150‑1 (MQ=255)

                                                                                                                                              gACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt    <  2:3878059/150‑1 (MQ=255)

                                                                                                                                               aCTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc   >  2:3119759/1‑150 (MQ=255)

                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt  <  1:2482105/150‑1 (MQ=255)

                                                                                                                                                cTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt  >  2:4096315/1‑150 (MQ=255)

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A