Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 732,494 G→T 86.8% pseudogene (214/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273732,4940GT86.8% 666.6 / 109.2 271pseudogene (214/1137 nt)ybfLpseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base T (139/96);  total (156/115)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  >  CP009273/732345‑732641
                                                                                                                                                     |                                                                                                                                                   
  gatcATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTAt                                                                                                                                                   >  2:3354410/4‑150 (MQ=255)
  gagCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGAt                                                                                                                                                   <  1:1564382/150‑1 (MQ=255)
     cATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACt                                                                                                                                                <  2:2698664/150‑1 (MQ=255)
     cATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACt                                                                                                                                                >  2:2433950/1‑150 (MQ=255)
      aTAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTa                                                                                                                                               <  2:295486/150‑1 (MQ=255)
         aaTTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTg                                                                                                                                            <  2:2147360/150‑1 (MQ=255)
         aaTTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTg                                                                                                                                            <  2:1615761/150‑1 (MQ=255)
              tCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCaga                                                                                                                                       >  1:2620073/1‑150 (MQ=255)
                 ggCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGtt                                                                                                                                    >  1:1139698/1‑150 (MQ=255)
                    aTCCTACTAGTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTa                                                                                                                                 <  2:4490336/150‑1 (MQ=255)
                        tactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCt                                                                                                                             <  1:3831989/150‑1 (MQ=255)
                         actaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCATg                                                                                                                            <  2:2617062/150‑1 (MQ=255)
                           taTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTa                                                                                                                          >  2:350128/1‑150 (MQ=255)
                                  tATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTcc                                                                                                                   >  1:2244995/1‑150 (MQ=255)
                                   aTTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCt                                                                                                                  <  1:3397960/150‑1 (MQ=255)
                                    tttttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTg                                                                                                                 <  2:1750923/150‑1 (MQ=255)
                                     ttttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGc                                                                                                                <  2:1034960/150‑1 (MQ=255)
                                     ttttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGc                                                                                                                <  1:562680/150‑1 (MQ=255)
                                         gCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaaa                                                                                                            <  1:630293/150‑1 (MQ=255)
                                         gCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaaa                                                                                                            >  1:2301439/1‑150 (MQ=255)
                                          ccGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAt                                                                                                           <  2:609868/150‑1 (MQ=255)
                                           cGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAtt                                                                                                          >  1:2458371/1‑150 (MQ=39)
                                            gTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAttt                                                                                                         >  2:2442307/1‑150 (MQ=255)
                                             ttATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTc                                                                                                        >  2:2755796/1‑150 (MQ=255)
                                             ttATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTc                                                                                                        >  1:3984928/1‑150 (MQ=255)
                                               aTTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCAc                                                                                                      <  1:1333831/150‑1 (MQ=255)
                                                     ggTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGc                                                                                                <  1:3498671/150‑1 (MQ=255)
                                                        gCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCttt                                                                                             >  2:2856948/1‑150 (MQ=255)
                                                          aGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                           >  1:3200866/1‑150 (MQ=255)
                                                           gAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttatt                                                                                          >  1:2448348/1‑150 (MQ=255)
                                                           gAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttatt                                                                                          <  2:3278670/150‑1 (MQ=255)
                                                              aGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAAc                                                                                       <  2:687069/150‑1 (MQ=255)
                                                                 tGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTgg                                                                                    <  2:3463759/150‑1 (MQ=37)
                                                                    gAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATg                                                                                 >  1:2737973/1‑150 (MQ=37)
                                                                      aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCg                                                                               >  2:2875090/1‑150 (MQ=37)
                                                                       aatatAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGt                                                                              >  2:122149/2‑150 (MQ=37)
                                                                       aatatAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGt                                                                              >  1:2606838/2‑150 (MQ=37)
                                                                        atatAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTg                                                                             >  2:3561999/1‑150 (MQ=37)
                                                                          ataGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAt                                                                           >  1:1949191/1‑150 (MQ=38)
                                                                           taGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAtt                                                                          <  2:546113/150‑1 (MQ=38)
                                                                           taGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAtt                                                                          <  1:683346/150‑1 (MQ=38)
                                                                            agagGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTg                                                                         >  1:2658380/1‑150 (MQ=255)
                                                                             gagGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGc                                                                        >  2:147020/1‑150 (MQ=255)
                                                                             gagGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGc                                                                        >  1:4419343/1‑150 (MQ=39)
                                                                                  tttCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtc                                                                   >  2:3678562/1‑150 (MQ=255)
                                                                                  ttccGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtc                                                                   <  1:3632268/147‑1 (MQ=38)
                                                                                    tCGGGGAAACACATCTCGATTTCTTGTAGCAATATGGGGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcat                                                                 <  1:1266016/150‑1 (MQ=255)
                                                                                     cGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatc                                                                >  1:3009933/1‑150 (MQ=255)
                                                                                          aaaCACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATg                                                           <  2:2413378/150‑1 (MQ=255)
                                                                                              acaTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATaa                                                       >  2:3926624/1‑150 (MQ=255)
                                                                                                aTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAg                                                     <  2:3795705/150‑1 (MQ=255)
                                                                                                   tcGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACg                                                  >  1:792845/1‑150 (MQ=255)
                                                                                                     gATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTc                                                >  1:2966384/1‑150 (MQ=255)
                                                                                                     gATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTc                                                >  2:2490757/1‑150 (MQ=255)
                                                                                                      aTTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCa                                               >  1:949223/1‑150 (MQ=255)
                                                                                                      aTTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCa                                               >  1:3698257/1‑150 (MQ=255)
                                                                                                       tttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAt                                              <  2:383781/150‑1 (MQ=255)
                                                                                                       tttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAt                                              >  1:1845596/1‑150 (MQ=255)
                                                                                                          cTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGc                                           >  1:1776425/1‑150 (MQ=40)
                                                                                                           ttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCa                                          <  2:3080082/150‑1 (MQ=255)
                                                                                                             gAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAt                                        >  1:4581278/1‑150 (MQ=255)
                                                                                                                gCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGa                                     <  2:815354/150‑1 (MQ=255)
                                                                                                                 cAATATGGTGATTTTGATAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGATTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAt                                    <  1:879409/150‑1 (MQ=255)
                                                                                                                 cAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAt                                    <  1:1421450/150‑1 (MQ=255)
                                                                                                                   atatGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATgg                                  >  2:3681654/1‑150 (MQ=39)
                                                                                                                    tatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGa                                 >  2:431428/1‑150 (MQ=255)
                                                                                                                    tatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGa                                 >  1:3409309/1‑150 (MQ=255)
                                                                                                                      tGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaa                               >  2:649159/1‑150 (MQ=255)
                                                                                                                       ggTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaaa                              >  2:4604173/1‑150 (MQ=255)
                                                                                                                        gTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaaaa                             >  1:3199249/1‑150 (MQ=255)
                                                                                                                         tGATTTTGAAAATTGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAc                            <  1:2508451/150‑1 (MQ=255)
                                                                                                                         tGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAc                            >  1:2150088/1‑150 (MQ=255)
                                                                                                                         tGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAc                            <  2:1792260/150‑1 (MQ=255)
                                                                                                                          gATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACg                           >  1:2357106/1‑150 (MQ=255)
                                                                                                                          gATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACg                           >  2:1869928/1‑150 (MQ=255)
                                                                                                                          gATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACg                           >  2:4380411/1‑150 (MQ=255)
                                                                                                                            ttttGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCt                         <  1:2856948/150‑1 (MQ=255)
                                                                                                                              ttGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTcc                       <  1:3262404/150‑1 (MQ=255)
                                                                                                                               tGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCg                      >  2:1436692/1‑150 (MQ=255)
                                                                                                                                gAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCgg                     >  2:1992147/1‑150 (MQ=255)
                                                                                                                                gAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCgg                     <  1:3129106/150‑1 (MQ=255)
                                                                                                                                  aaaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCa                   <  2:2020334/150‑1 (MQ=255)
                                                                                                                                  aaaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCa                   >  2:1843946/1‑150 (MQ=255)
                                                                                                                                  aaaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCa                   >  2:3085104/1‑150 (MQ=255)
                                                                                                                                   aaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAc                  >  2:3717957/1‑150 (MQ=255)
                                                                                                                                   aaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAc                  >  1:2585584/1‑150 (MQ=255)
                                                                                                                                     tGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCActc                >  2:3420445/1‑150 (MQ=255)
                                                                                                                                     tGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCActc                >  1:2820787/1‑150 (MQ=255)
                                                                                                                                        tATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa             <  2:3115066/150‑1 (MQ=255)
                                                                                                                                         aTTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAt            >  2:399626/1‑150 (MQ=255)
                                                                                                                                           tCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGa          >  2:220601/1‑150 (MQ=255)
                                                                                                                                            ccTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGAc         <  2:2634526/150‑1 (MQ=255)
                                                                                                                                             cTGTACACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATTCGTGATTGCCATTCATCAAATGATAAATACTTCATTGCAATTGATGGAAAAACTCTCCGGCACTCTTATGaaa        >  1:597712/1‑148 (MQ=14)
                                                                                                                                               gTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAag      >  1:4271378/1‑150 (MQ=255)
                                                                                                                                                ttCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAaga     <  2:4010312/150‑1 (MQ=255)
                                                                                                                                                 tCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAagag    >  1:2115480/1‑150 (MQ=255)
                                                                                                                                                  cACTATACTATTGCCAGAGTTGTATCCTGTATCAGTACTGCAAAATTACACGAGTGATTTATTAAATGTATGAGAGATTGCCAAACATCAAATGATAAATACGTCAATGCAATTGATGGAAAAACGCACCGGCACTCTTATGACAagaga   >  1:3592518/1‑149 (MQ=14)
                                                                                                                                                   aCGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTc  <  2:388127/150‑1 (MQ=39)
                                                                                                                                                   aCGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTc  <  1:4259412/150‑1 (MQ=39)
                                                                                                                                                   aCGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTc  <  2:17759/150‑1 (MQ=39)
                                                                                                                                                     |                                                                                                                                                   
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  >  CP009273/732345‑732641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  >  CP009273/732345‑732641
                                                                                                                                                     |                                                                                                                                                   
TAGATCATAAATTGTCAGGCATCCTACTATTGACTATTGTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACT                                                                                                                                                     >  SRR19973072.2359632/1‑150 (MQ=60)
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACT                                                                                                                                                     <  SRR19973072.1201051/150‑1 (MQ=60)
 AGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTA                                                                                                                                                    <  SRR19973072.3627127/150‑1 (MQ=60)
  GAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGAT                                                                                                                                                   <  SRR19973072.1567720/150‑1 (MQ=60)
   ATCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATA                                                                                                                                                  >  SRR19973072.627332/1‑150 (MQ=60)
   AGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATA                                                                                                                                                  >  SRR19973072.3869462/1‑150 (MQ=60)
      ATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTA                                                                                                                                               >  SRR19973072.4378024/1‑150 (MQ=60)
       TAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTAT                                                                                                                                              >  SRR19973072.731721/1‑150 (MQ=60)
          ATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGC                                                                                                                                           <  SRR19973072.3038013/150‑1 (MQ=60)
           TTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCC                                                                                                                                          <  SRR19973072.937060/150‑1 (MQ=60)
             GTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATAGTATTCCTGTTCACTATACTATTGCCAG                                                                                                                                        <  SRR19973072.3238455/150‑1 (MQ=60)
              TCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGA                                                                                                                                       >  SRR19973072.2625713/1‑150 (MQ=60)
                AGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTAATCCTGTTCACGATACTATTGCCAGAGT                                                                                                                                     <  SRR19973072.4119408/150‑1 (MQ=60)
                 GGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTT                                                                                                                                    >  SRR19973072.1142080/1‑150 (MQ=60)
                     TCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTAT                                                                                                                                <  SRR19973072.1519901/150‑1 (MQ=58)
                     TCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTAT                                                                                                                                >  SRR19973072.1882323/1‑150 (MQ=55)
                        TACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCT                                                                                                                             <  SRR19973072.3839997/150‑1 (MQ=53)
                                  TATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCC                                                                                                                   >  SRR19973072.2249837/1‑150 (MQ=51)
                                   ATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCT                                                                                                                  <  SRR19973072.3405161/150‑1 (MQ=54)
                                   ATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCT                                                                                                                  <  SRR19973072.1381097/150‑1 (MQ=54)
                                   ATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCT                                                                                                                  <  SRR19973072.3405138/150‑1 (MQ=54)
                                     TTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGC                                                                                                                <  SRR19973072.564000/150‑1 (MQ=50)
                                        TGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAA                                                                                                             >  SRR19973072.1390607/1‑150 (MQ=42)
                                        TGCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAA                                                                                                             <  SRR19973072.3668052/150‑1 (MQ=41)
                                         GCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAA                                                                                                            <  SRR19973072.4308595/150‑1 (MQ=60)
                                         GCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAA                                                                                                            <  SRR19973072.631734/150‑1 (MQ=60)
                                         GCCGTTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAA                                                                                                            >  SRR19973072.2306355/1‑150 (MQ=41)
                                          CCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAT                                                                                                           <  SRR19973072.1309975/150‑1 (MQ=43)
                                           CGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATT                                                                                                          >  SRR19973072.2463710/1‑150 (MQ=43)
                                           CGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATT                                                                                                          >  SRR19973072.2463761/1‑150 (MQ=43)
                                             TTATTTCTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTC                                                                                                        >  SRR19973072.3993268/1‑150 (MQ=41)
                                              TATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCA                                                                                                       >  SRR19973072.2453175/1‑150 (MQ=42)
                                               ATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCAC                                                                                                      <  SRR19973072.2972920/150‑1 (MQ=60)
                                               ATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCAC                                                                                                      <  SRR19973072.1336748/150‑1 (MQ=60)
                                                   CTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGT                                                                                                  >  SRR19973072.2287247/1‑150 (MQ=41)
                                                   CTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGT                                                                                                  >  SRR19973072.2236822/1‑150 (MQ=41)
                                                   CTGGTGCAGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGT                                                                                                  >  SRR19973072.4652236/1‑150 (MQ=41)
                                                     GGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGC                                                                                                <  SRR19973072.3506000/150‑1 (MQ=42)
                                                        GCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTT                                                                                             <  SRR19973072.2556924/150‑1 (MQ=42)
                                                        GCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTT                                                                                             <  SRR19973072.2288675/150‑1 (MQ=42)
                                                        GCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTT                                                                                             <  SRR19973072.4627892/150‑1 (MQ=42)
                                                          AGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                           <  SRR19973072.4526646/150‑1 (MQ=42)
                                                          AGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                           >  SRR19973072.3207587/1‑150 (MQ=41)
                                                          AGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                           >  SRR19973072.3207623/1‑150 (MQ=41)
                                                          AGAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                           >  SRR19973072.3207628/1‑150 (MQ=41)
                                                           GAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCTGTCCTGCAAAATTTCACGAGTGCTTgagt                                                                                          >  SRR19973072.2725333/1‑146 (MQ=39)
                                                           GAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATT                                                                                          >  SRR19973072.2453752/1‑150 (MQ=40)
                                                           GAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATT                                                                                          >  SRR19973072.2453670/1‑150 (MQ=40)
                                                           GAAAGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATT                                                                                          <  SRR19973072.2833508/150‑1 (MQ=39)
                                                            AAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTA                                                                                         >  SRR19973072.2640182/1‑150 (MQ=38)
                                                            AAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTA                                                                                         >  SRR19973072.2640042/1‑150 (MQ=38)
                                                              AGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAAC                                                                                       <  SRR19973072.4512376/150‑1 (MQ=34)
                                                              AGTTGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAAC                                                                                       <  SRR19973072.2330002/150‑1 (MQ=33)
                                                               GTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACT                                                                                      <  SRR19973072.2898070/150‑1 (MQ=32)
                                                                 TGGGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGG                                                                                    >  SRR19973072.2053385/1‑150 (MQ=31)
                                                                   GGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAT                                                                                  <  SRR19973072.2787307/150‑1 (MQ=32)
                                                                    GAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATG                                                                                 >  SRR19973072.2743816/1‑150 (MQ=32)
                                                                       AATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGT                                                                              >  SRR19973072.2612559/1‑150 (MQ=31)
                                                                       AATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGT                                                                              >  SRR19973072.2612557/1‑150 (MQ=31)
                                                                       AATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGT                                                                              >  SRR19973072.2612457/1‑150 (MQ=31)
                                                                         TATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGA                                                                            <  SRR19973072.2606072/150‑1 (MQ=32)
                                                                         TATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGA                                                                            <  SRR19973072.285206/150‑1 (MQ=32)
                                                                          ATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGTTGATTTTGAAAATGGTATTCCTGTTCAATATACTATTGCCAGAGTTGTATCCTGTCTCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAT                                                                           >  SRR19973072.1718101/1‑150 (MQ=31)
                                                                          ATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAT                                                                           >  SRR19973072.1953350/1‑150 (MQ=34)
                                                                           TAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATT                                                                          <  SRR19973072.684859/150‑1 (MQ=36)
                                                                            AGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTG                                                                         >  SRR19973072.2664140/1‑150 (MQ=38)
                                                                            AGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTG                                                                         >  SRR19973072.2664075/1‑150 (MQ=38)
                                                                            AGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTG                                                                         <  SRR19973072.1320780/150‑1 (MQ=38)
                                                                             GAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGC                                                                        >  SRR19973072.4428646/1‑150 (MQ=41)
                                                                                  TTCCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTC                                                                   <  SRR19973072.3639722/150‑1 (MQ=34)
                                                                                  TTCCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTC                                                                   <  SRR19973072.3639615/150‑1 (MQ=34)
                                                                                  TTCCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTC                                                                   <  SRR19973072.3639919/150‑1 (MQ=34)
                                                                                   TTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCA                                                                  >  SRR19973072.1956780/1‑150 (MQ=41)
                                                                                    TCGGGGAAACACATCTCGATTTCTTGTAGCAATATGGGGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCAT                                                                 <  SRR19973072.1268742/150‑1 (MQ=38)
                                                                                     CGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATC                                                                >  SRR19973072.3016297/1‑150 (MQ=40)
                                                                                     CGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATC                                                                >  SRR19973072.3016331/1‑150 (MQ=40)
                                                                                     CGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATC                                                                >  SRR19973072.3930278/1‑150 (MQ=40)
                                                                                       GGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAA                                                              <  SRR19973072.1462556/150‑1 (MQ=42)
                                                                                        GGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAA                                                             >  SRR19973072.1185016/1‑150 (MQ=44)
                                                                                         GAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAT                                                            <  SRR19973072.2344216/150‑1 (MQ=46)
                                                                                         GAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAT                                                            <  SRR19973072.3759761/150‑1 (MQ=46)
                                                                                            ACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAT                                                         <  SRR19973072.372591/150‑1 (MQ=53)
                                                                                                   TCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACG                                                  >  SRR19973072.794537/1‑150 (MQ=53)
                                                                                                   TCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACG                                                  >  SRR19973072.794721/1‑150 (MQ=53)
                                                                                                     GATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTC                                                >  SRR19973072.2972704/1‑150 (MQ=53)
                                                                                                     GATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTC                                                >  SRR19973072.2972716/1‑150 (MQ=53)
                                                                                                     GATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTC                                                >  SRR19973072.3574761/1‑150 (MQ=53)
                                                                                                     GATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTC                                                >  SRR19973072.3574748/1‑150 (MQ=53)
                                                                                                      ATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCA                                               >  SRR19973072.951301/1‑150 (MQ=53)
                                                                                                      ATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCA                                               >  SRR19973072.3706053/1‑150 (MQ=53)
                                                                                                      ATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCA                                               >  SRR19973072.951296/1‑150 (MQ=53)
                                                                                                       TTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAT                                              >  SRR19973072.1849595/1‑150 (MQ=50)
                                                                                                       TTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAT                                              >  SRR19973072.2545568/1‑150 (MQ=50)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATT                                             <  SRR19973072.1901336/150‑1 (MQ=48)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATT                                             >  SRR19973072.3392838/1‑150 (MQ=48)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATACATCAAATGATAAAGACGTCAAT                                             >  SRR19973072.1815669/1‑150 (MQ=47)
                                                                                                          CTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGC                                           >  SRR19973072.1780958/1‑150 (MQ=45)
                                                                                                          CTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGC                                           >  SRR19973072.1780313/1‑150 (MQ=45)
                                                                                                             GAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAT                                        >  SRR19973072.4590910/1‑150 (MQ=42)
                                                                                                                 CAATATGGTGATTTTGATAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGATTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAT                                    <  SRR19973072.881299/150‑1 (MQ=40)
                                                                                                                 CAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAT                                    >  SRR19973072.3484646/1‑150 (MQ=42)
                                                                                                                 CAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAT                                    <  SRR19973072.1424532/150‑1 (MQ=42)
                                                                                                                    TATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGA                                 >  SRR19973072.3416504/1‑150 (MQ=42)
                                                                                                                     ATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAA                                <  SRR19973072.3441541/150‑1 (MQ=43)
                                                                                                                        GTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAA                             >  SRR19973072.3206007/1‑150 (MQ=42)
                                                                                                                         TGATTTTGAAAATTGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAC                            <  SRR19973072.2513903/150‑1 (MQ=41)
                                                                                                                         TGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAC                            >  SRR19973072.3274160/1‑150 (MQ=42)
                                                                                                                         TGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAC                            >  SRR19973072.65100/1‑150 (MQ=42)
                                                                                                                         TGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAC                            >  SRR19973072.64944/1‑150 (MQ=42)
                                                                                                                         TGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAC                            >  SRR19973072.2154768/1‑150 (MQ=42)
                                                                                                                          GATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACG                           >  SRR19973072.2362181/1‑150 (MQ=42)
                                                                                                                            TTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCT                         <  SRR19973072.2863066/150‑1 (MQ=42)
                                                                                                                              TTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCC                       <  SRR19973072.3269327/150‑1 (MQ=42)
                                                                                                                               TGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCG                      >  SRR19973072.597551/1‑150 (MQ=42)
                                                                                                                                GAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGG                     <  SRR19973072.3135709/150‑1 (MQ=42)
                                                                                                                                 AAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGC                    >  SRR19973072.3047646/1‑150 (MQ=42)
                                                                                                                                   AATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAC                  >  SRR19973072.326399/1‑150 (MQ=42)
                                                                                                                                   AATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAC                  >  SRR19973072.2591166/1‑150 (MQ=42)
                                                                                                                                     TGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTC                >  SRR19973072.2826829/1‑150 (MQ=42)
                                                                                                                                      GGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCT               >  SRR19973072.2463161/1‑150 (MQ=42)
                                                                                                                                       GTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTT              >  SRR19973072.4217791/1‑150 (MQ=42)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA             >  SRR19973072.3183884/1‑150 (MQ=42)
                                                                                                                                         ATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAT            <  SRR19973072.2159859/150‑1 (MQ=42)
                                                                                                                                         ATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAT            <  SRR19973072.699523/150‑1 (MQ=42)
                                                                                                                                             CTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACA        >  SRR19973072.599113/1‑150 (MQ=42)
                                                                                                                                             CTGTACACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATTCGTGATTGCCATTCATCAAATGATAAATACTTCATTGCAATTGATGGAAAAACTCTCCGGCACTCTTATGAAA        >  SRR19973072.599094/1‑150 (MQ=36)
                                                                                                                                               GTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAG      >  SRR19973072.4280263/1‑150 (MQ=42)
                                                                                                                                                 TCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGGG    >  SRR19973072.2120147/1‑150 (MQ=42)
                                                                                                                                                 TCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAG    >  SRR19973072.2120091/1‑150 (MQ=42)
                                                                                                                                                  CACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATTACAAGAGT   >  SRR19973072.2482915/1‑150 (MQ=41)
                                                                                                                                                  CACTATACTATTGCCAGAGTTGTATCCTGTATCAGTACTGCAAAATTACACGAGTGATTTATTAAATGTATGAGAGATTGCCAAACATCAAATGATAAATACGTCAATGCAATTGATGGAAAAACGCACCGGCACTCTTATGACAAGAGA   >  SRR19973072.3600078/1‑150 (MQ=30)
                                                                                                                                                   ACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  >  SRR19973072.222510/1‑150 (MQ=42)
                                                                                                                                                   ACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  <  SRR19973072.4268353/150‑1 (MQ=43)
                                                                                                                                                   ACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  <  SRR19973072.4268265/150‑1 (MQ=43)
                                                                                                                                                     |                                                                                                                                                   
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC  >  CP009273/732345‑732641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑