Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 732,494 G→T 100% pseudogene (214/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273732,4940GT95.6% 746.4 / 21.7 249pseudogene (214/1137 nt)ybfLpseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
Reads supporting (aligned to +/- strand):  ref base G (7/4);  new base T (115/123);  total (122/127)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 252 total aligned reads displayed.

AAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGA  >  CP009273/732353‑732638
                                                                                                                                             |                                                                                                                                                
aaaTTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTa                                                                                                                                            >  1:3357594/1‑148 (MQ=255)
 aaTTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCactatactat                                                                                                                                           >  2:2126088/1‑148 (MQ=255)
 aaTTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCactatactat                                                                                                                                           <  2:3547187/148‑1 (MQ=255)
  aTTGTTAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTAtt                                                                                                                                          <  2:642095/148‑1 (MQ=255)
   ttGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTg                                                                                                                                         <  1:717693/148‑1 (MQ=255)
   ttGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTg                                                                                                                                         <  1:3129897/148‑1 (MQ=255)
       cAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCag                                                                                                                                     <  2:507487/148‑1 (MQ=255)
       cAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCag                                                                                                                                     <  2:2363564/148‑1 (MQ=255)
         ggCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCagag                                                                                                                                   >  2:3311875/1‑148 (MQ=255)
         ggCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCagag                                                                                                                                   >  1:238902/1‑148 (MQ=255)
          gCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGt                                                                                                                                  >  2:2627656/1‑148 (MQ=255)
              ccTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTa                                                                                                                              <  1:1015303/148‑1 (MQ=255)
              ccTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTa                                                                                                                              >  1:2185987/1‑148 (MQ=255)
               ctactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTAt                                                                                                                             <  2:1022945/148‑1 (MQ=255)
                tactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATc                                                                                                                            <  2:1951523/148‑1 (MQ=255)
                   taTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTg                                                                                                                         >  1:1619346/1‑148 (MQ=255)
                   taTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTg                                                                                                                         >  2:1618207/1‑148 (MQ=255)
                    aTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGt                                                                                                                        >  2:3414077/1‑148 (MQ=255)
                       gACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATc                                                                                                                     <  2:2650197/148‑1 (MQ=255)
                          tATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGt                                                                                                                  >  2:1266902/1‑148 (MQ=255)
                           aTTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTc                                                                                                                 <  2:1485971/148‑1 (MQ=255)
                           aTTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTc                                                                                                                 <  2:1692554/148‑1 (MQ=255)
                           aTTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTATTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTc                                                                                                                 >  2:152953/1‑148 (MQ=255)
                             ttttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCt                                                                                                               >  2:2911618/1‑148 (MQ=255)
                             ttttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCt                                                                                                               >  1:2644054/1‑148 (MQ=255)
                             ttttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCt                                                                                                               <  1:2811965/148‑1 (MQ=255)
                                tGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCa                                                                                                            <  1:614018/148‑1 (MQ=255)
                                  ccGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaa                                                                                                          <  2:1776399/148‑1 (MQ=255)
                                  ccGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaa                                                                                                          >  1:1157854/1‑148 (MQ=255)
                                   cGTTATTTCTGGTGCAGAAAGTTGTGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaaa                                                                                                         <  2:431925/148‑1 (MQ=255)
                                   cGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaaa                                                                                                         <  1:1348194/148‑1 (MQ=255)
                                         ttCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCAc                                                                                                   >  2:1986773/1‑148 (MQ=255)
                                           cTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGa                                                                                                 <  2:1566113/148‑1 (MQ=255)
                                               tGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGc                                                                                             <  2:3206120/148‑1 (MQ=255)
                                               tGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGc                                                                                             <  2:2831699/148‑1 (MQ=255)
                                                   gAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTtta                                                                                         <  2:2261213/148‑1 (MQ=255)
                                                    aaaGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                        >  1:299426/1‑148 (MQ=255)
                                                    aaaGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttat                                                                                        >  1:1727411/1‑148 (MQ=255)
                                                     aaGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTttatt                                                                                       <  1:420141/148‑1 (MQ=38)
                                                       gTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTaa                                                                                     <  1:2271509/148‑1 (MQ=37)
                                                        ttGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAAc                                                                                    >  2:158544/1‑148 (MQ=37)
                                                            gAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGa                                                                                <  1:378597/148‑1 (MQ=37)
                                                             aaGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGGTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTGTATTAACTGGAt                                                                               >  2:3290829/1‑148 (MQ=255)
                                                              aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATg                                                                              <  2:843206/148‑1 (MQ=37)
                                                              aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATg                                                                              >  1:2595081/1‑148 (MQ=37)
                                                              aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATg                                                                              <  1:2933211/148‑1 (MQ=37)
                                                              aGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATg                                                                              <  1:2927245/148‑1 (MQ=37)
                                                               gATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGc                                                                             >  1:3310004/1‑148 (MQ=37)
                                                               gATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGc                                                                             <  1:1561707/148‑1 (MQ=37)
                                                               gATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGc                                                                             <  1:2882471/148‑1 (MQ=37)
                                                                  ataGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTg                                                                          >  1:64388/1‑148 (MQ=37)
                                                                   taGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTGTATTAACTGGATGCGTGa                                                                         >  2:2813816/1‑148 (MQ=255)
                                                                    agagGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAt                                                                        >  1:441012/1‑148 (MQ=38)
                                                                     gagGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAtt                                                                       >  2:417073/1‑148 (MQ=38)
                                                                      agGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTg                                                                      >  2:3515302/1‑148 (MQ=255)
                                                                            tctgggAAACACATATCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGGATCCTGTATCAGTCCTGCAAAATTTCCCGAGTGATTTATTCACTGGATGCGTGATTGCCATtc                                                                <  1:1238385/145‑1 (MQ=11)
                                                                               gggAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatc                                                             >  1:3332853/1‑148 (MQ=255)
                                                                                ggAAACACATCTCGATTTCTTGAAGCAATATTGTGATTTTCAAAATCGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGAATCAGTCCTGCAAAATTTCACGACTGATTTATTAACTGGATGCGTGATAGCCATtcatca                                                            >  1:1910704/1‑148 (MQ=14)
                                                                                ggAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatca                                                            >  2:3380141/1‑148 (MQ=255)
                                                                                 gAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCaa                                                           <  1:204563/148‑1 (MQ=255)
                                                                                 gAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCaa                                                           <  2:185091/148‑1 (MQ=255)
                                                                                    acacATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATg                                                        >  1:3043924/1‑148 (MQ=255)
                                                                                       caTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATa                                                     <  1:892030/148‑1 (MQ=255)
                                                                                          ctcGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAg                                                  >  2:1135500/1‑148 (MQ=255)
                                                                                            cGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGAc                                                >  2:2455130/1‑148 (MQ=255)
                                                                                              aTTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGt                                              >  2:2305068/1‑148 (MQ=255)
                                                                                               tttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTc                                             <  2:3220485/148‑1 (MQ=255)
                                                                                               tttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTc                                             <  2:784714/148‑1 (MQ=255)
                                                                                                ttCTTGAATCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCa                                            <  2:2418997/148‑1 (MQ=255)
                                                                                                ttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCa                                            >  1:447533/1‑148 (MQ=255)
                                                                                                    tGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGc                                        >  1:1006187/1‑148 (MQ=255)
                                                                                                     gAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCa                                       <  1:1258592/148‑1 (MQ=255)
                                                                                                       aGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAt                                     >  2:740318/1‑148 (MQ=255)
                                                                                                       aGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAt                                     <  1:2126088/148‑1 (MQ=255)
                                                                                                       aGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAt                                     <  2:1800093/148‑1 (MQ=255)
                                                                                                          aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGa                                  <  2:479622/148‑1 (MQ=255)
                                                                                                          aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGa                                  <  2:3352059/148‑1 (MQ=255)
                                                                                                          aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGa                                  <  2:1913644/148‑1 (MQ=255)
                                                                                                          aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGa                                  <  1:1616706/148‑1 (MQ=255)
                                                                                                            tatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATg                                >  2:1264732/1‑148 (MQ=255)
                                                                                                            tatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATg                                >  2:1540575/1‑148 (MQ=255)
                                                                                                             atGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATgg                               >  2:2988179/1‑148 (MQ=255)
                                                                                                              tGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGa                              >  1:2134302/1‑148 (MQ=255)
                                                                                                                 tGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaaa                           >  2:3390634/1‑148 (MQ=255)
                                                                                                                  gATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaaaa                          <  1:340445/148‑1 (MQ=255)
                                                                                                                        gAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTcc                    <  2:635541/148‑1 (MQ=255)
                                                                                                                          aaaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCgg                  <  1:1266902/148‑1 (MQ=255)
                                                                                                                            aTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCa                >  2:1684791/1‑148 (MQ=255)
                                                                                                                             tGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAc               >  2:1171574/1‑148 (MQ=255)
                                                                                                                              ggTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAct              <  2:2857823/148‑1 (MQ=255)
                                                                                                                              ggTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCAct              <  2:2857339/148‑1 (MQ=255)
                                                                                                                               gTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCActc             <  1:1500053/148‑1 (MQ=255)
                                                                                                                                 aTTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCtt           <  1:3157338/148‑1 (MQ=255)
                                                                                                                                 aTTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCtt           >  1:282386/1‑148 (MQ=255)
                                                                                                                                  ttCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa          <  1:821228/148‑1 (MQ=255)
                                                                                                                                  ttCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa          <  2:2635205/148‑1 (MQ=255)
                                                                                                                                     cTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGa       >  1:935264/1‑148 (MQ=255)
                                                                                                                                      tGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGAc      <  1:188566/148‑1 (MQ=255)
                                                                                                                                       gTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACa     <  1:2089023/148‑1 (MQ=255)
                                                                                                                                       gTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACa     >  1:2459410/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
AAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGA  >  CP009273/732353‑732638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG  >  CP009273/732345‑732642
                                                                                                                                                     |                                                                                                                                                    
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACT                                                                                                                                                      <  SRR19973066.2768979/150‑1 (MQ=60)
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACT                                                                                                                                                      <  SRR19973066.2766739/150‑1 (MQ=60)
 AGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTA                                                                                                                                                     >  SRR19973066.749886/1‑150 (MQ=60)
      ATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTA                                                                                                                                                >  SRR19973066.3379974/1‑150 (MQ=60)
          ATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGC                                                                                                                                            >  SRR19973066.3326066/1‑150 (MQ=60)
           TTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCC                                                                                                                                           <  SRR19973066.3150792/150‑1 (MQ=60)
           TTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCC                                                                                                                                           <  SRR19973066.3151192/150‑1 (MQ=60)
           TTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCC                                                                                                                                           <  SRR19973066.723003/150‑1 (MQ=60)
               CAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAG                                                                                                                                       >  SRR19973066.240777/1‑150 (MQ=60)
                AGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGT                                                                                                                                      <  SRR19973066.3336544/150‑1 (MQ=60)
                AGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGT                                                                                                                                      <  SRR19973066.2990539/150‑1 (MQ=60)
                 GGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTT                                                                                                                                     <  SRR19973066.2400558/150‑1 (MQ=60)
                 GGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTT                                                                                                                                     <  SRR19973066.2400532/150‑1 (MQ=60)
                    ATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTA                                                                                                                                  >  SRR19973066.2201155/1‑150 (MQ=59)
                    ATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTA                                                                                                                                  >  SRR19973066.2201140/1‑150 (MQ=59)
                    ATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTA                                                                                                                                  >  SRR19973066.2201139/1‑150 (MQ=59)
                      CCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATC                                                                                                                                >  SRR19973066.1283705/1‑150 (MQ=55)
                      CCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATC                                                                                                                                <  SRR19973066.1022776/150‑1 (MQ=55)
                        TACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCT                                                                                                                              <  SRR19973066.3489040/150‑1 (MQ=55)
                         ACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTG                                                                                                                             >  SRR19973066.2502965/1‑150 (MQ=55)
                         ACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTG                                                                                                                             >  SRR19973066.1631084/1‑150 (MQ=55)
                         ACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTG                                                                                                                             >  SRR19973066.1631023/1‑150 (MQ=55)
                                 CTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTC                                                                                                                     <  SRR19973066.1921307/150‑1 (MQ=53)
                                   ATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCT                                                                                                                   >  SRR19973066.2661826/1‑150 (MQ=60)
                                     TTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGC                                                                                                                 <  SRR19973066.2830876/150‑1 (MQ=60)
                                       TTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAA                                                                                                               >  SRR19973066.2103100/1‑150 (MQ=60)
                                        TGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAA                                                                                                              >  SRR19973066.1166082/1‑150 (MQ=42)
                                        TGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAA                                                                                                              <  SRR19973066.618318/150‑1 (MQ=60)
                                         GCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAA                                                                                                             >  SRR19973066.3378739/1‑150 (MQ=42)
                                           CGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATT                                                                                                           <  SRR19973066.1357972/150‑1 (MQ=60)
                                           CGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATT                                                                                                           <  SRR19973066.44724/150‑1 (MQ=60)
                                            GTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTT                                                                                                          <  SRR19973066.4988/150‑1 (MQ=60)
                                              TATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCA                                                                                                        >  SRR19973066.1566948/1‑150 (MQ=42)
                                              TATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCA                                                                                                        <  SRR19973066.1144225/150‑1 (MQ=60)
                                               ATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCAC                                                                                                       <  SRR19973066.2456838/150‑1 (MQ=60)
                                                   CTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCATGCAAAATTTCACGAGT                                                                                                   >  SRR19973066.3241794/1‑150 (MQ=60)
                                                       TGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTT                                                                                               <  SRR19973066.1260959/150‑1 (MQ=42)
                                                        GCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTT                                                                                              >  SRR19973066.2841915/1‑150 (MQ=42)
                                                          AGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                            >  SRR19973066.301538/1‑150 (MQ=42)
                                                          AGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                            >  SRR19973066.1739901/1‑150 (MQ=42)
                                                          AGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                            >  SRR19973066.2876692/1‑150 (MQ=42)
                                                          AGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTAT                                                                                            >  SRR19973066.1976153/1‑150 (MQ=42)
                                                             AAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAA                                                                                         <  SRR19973066.423412/150‑1 (MQ=36)
                                                               GTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACT                                                                                       <  SRR19973066.1629942/150‑1 (MQ=32)
                                                               GTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACT                                                                                       <  SRR19973066.2287123/150‑1 (MQ=32)
                                                                    GAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATG                                                                                  >  SRR19973066.2612659/1‑150 (MQ=32)
                                                                    GAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATG                                                                                  >  SRR19973066.2612531/1‑150 (MQ=32)
                                                                    GAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATG                                                                                  <  SRR19973066.381619/150‑1 (MQ=32)
                                                                    GAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATG                                                                                  <  SRR19973066.2047551/150‑1 (MQ=32)
                                                                    GAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATG                                                                                  <  SRR19973066.381578/150‑1 (MQ=32)
                                                                     AAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGC                                                                                 >  SRR19973066.3331956/1‑150 (MQ=32)
                                                                     AAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGC                                                                                 >  SRR19973066.2203122/1‑150 (MQ=31)
                                                                      AGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCG                                                                                <  SRR19973066.2952730/150‑1 (MQ=32)
                                                                      AGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCG                                                                                <  SRR19973066.2946741/150‑1 (MQ=32)
                                                                      AGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCG                                                                                <  SRR19973066.2952725/150‑1 (MQ=32)
                                                                       GATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGT                                                                               <  SRR19973066.1572950/150‑1 (MQ=32)
                                                                       GATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGT                                                                               <  SRR19973066.858154/150‑1 (MQ=32)
                                                                       GATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGT                                                                               <  SRR19973066.2901771/150‑1 (MQ=32)
                                                                        ATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTG                                                                              >  SRR19973066.65175/1‑150 (MQ=32)
                                                                          ATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAT                                                                            >  SRR19973066.444420/1‑150 (MQ=34)
                                                                          ATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAT                                                                            >  SRR19973066.444338/1‑150 (MQ=34)
                                                                          ATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAT                                                                            >  SRR19973066.444334/1‑150 (MQ=34)
                                                                              AGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCC                                                                        >  SRR19973066.973376/1‑150 (MQ=42)
                                                                                 ATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATT                                                                     >  SRR19973066.952023/1‑150 (MQ=42)
                                                                                   TTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCA                                                                   <  SRR19973066.1385180/150‑1 (MQ=40)
                                                                                    TCTGGGAAACACATATCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGGATCCTGTATCAGTCCTGCAAAATTTCCCGAGTGATTTATTCACTGGATGCGTGATTGCCATTCAT                                                                  <  SRR19973066.1247213/150‑1 (MQ=31)
                                                                                    TCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCAT                                                                  <  SRR19973066.3023588/150‑1 (MQ=40)
                                                                                     CGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATC                                                                 >  SRR19973066.3355014/1‑150 (MQ=40)
                                                                                      GGGGAAACACATCTCGATTTCTTGAAGCAATATTGTGATTTTCAAAATCGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGAATCAGTCCTGCAAAATTTCACGACTGATTTATTAACTGGATGCGTGATAGCCATTCATCA                                                                >  SRR19973066.1924309/1‑150 (MQ=31)
                                                                                       GGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAA                                                               >  SRR19973066.677218/1‑150 (MQ=42)
                                                                                         GAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAT                                                             <  SRR19973066.206288/150‑1 (MQ=46)
                                                                                          AAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATG                                                            >  SRR19973066.3064270/1‑150 (MQ=48)
                                                                                           AACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGA                                                           >  SRR19973066.750201/1‑150 (MQ=50)
                                                                                             CACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATA                                                         <  SRR19973066.1734465/150‑1 (MQ=53)
                                                                                               CATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAA                                                       <  SRR19973066.898347/150‑1 (MQ=53)
                                                                                                 TCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGA                                                     <  SRR19973066.3011749/150‑1 (MQ=53)
                                                                                                  CTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGAC                                                    >  SRR19973066.128521/1‑150 (MQ=54)
                                                                                                     GATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTC                                                 >  SRR19973066.1054646/1‑150 (MQ=53)
                                                                                                      ATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCA                                                >  SRR19973066.537751/1‑150 (MQ=53)
                                                                                                      ATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCA                                                >  SRR19973066.920536/1‑150 (MQ=53)
                                                                                                      ATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCA                                                >  SRR19973066.450981/1‑150 (MQ=53)
                                                                                                         TCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTG                                             >  SRR19973066.3447528/1‑150 (MQ=46)
                                                                                                          CTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGC                                            >  SRR19973066.1013600/1‑150 (MQ=44)
                                                                                                             GAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAT                                         <  SRR19973066.1267664/150‑1 (MQ=42)
                                                                                                             GAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAT                                         <  SRR19973066.1266153/150‑1 (MQ=42)
                                                                                                              AAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATT                                        <  SRR19973066.642882/150‑1 (MQ=42)
                                                                                                               AGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTG                                       <  SRR19973066.2140811/150‑1 (MQ=42)
                                                                                                                  AATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATG                                    <  SRR19973066.1627403/150‑1 (MQ=42)
                                                                                                                  AATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATG                                    <  SRR19973066.1628436/150‑1 (MQ=42)
                                                                                                                  AATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATG                                    >  SRR19973066.1224294/1‑150 (MQ=42)
                                                                                                                    TATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGA                                  >  SRR19973066.2148834/1‑150 (MQ=42)
                                                                                                                    TATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGA                                  >  SRR19973066.2149106/1‑150 (MQ=42)
                                                                                                                      TGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGCCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAA                                >  SRR19973066.3086345/1‑150 (MQ=42)
                                                                                                                          GATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACG                            <  SRR19973066.343065/150‑1 (MQ=42)
                                                                                                                           ATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGC                           >  SRR19973066.2838002/1‑150 (MQ=42)
                                                                                                                             TTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTC                         <  SRR19973066.2127642/150‑1 (MQ=42)
                                                                                                                             TTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTC                         <  SRR19973066.2128266/150‑1 (MQ=42)
                                                                                                                               TGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCG                       >  SRR19973066.1017509/1‑150 (MQ=42)
                                                                                                                                GAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGG                      >  SRR19973066.3363184/1‑150 (MQ=42)
                                                                                                                                 AAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGC                     >  SRR19973066.2696245/1‑150 (MQ=42)
                                                                                                                                  AAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCA                    <  SRR19973066.1275724/150‑1 (MQ=42)
                                                                                                                                  AAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCA                    <  SRR19973066.1276051/150‑1 (MQ=42)
                                                                                                                                  AAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCA                    >  SRR19973066.177971/1‑150 (MQ=43)
                                                                                                                                  AAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCA                    >  SRR19973066.177960/1‑150 (MQ=43)
                                                                                                                                    ATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACT                  >  SRR19973066.3139207/1‑150 (MQ=42)
                                                                                                                                       GTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTT               >  SRR19973066.284436/1‑150 (MQ=42)
                                                                                                                                       GTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTT               <  SRR19973066.1510508/150‑1 (MQ=42)
                                                                                                                                       GTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTT               <  SRR19973066.1510642/150‑1 (MQ=42)
                                                                                                                                         ATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAT             >  SRR19973066.2771476/1‑150 (MQ=42)
                                                                                                                                         ATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAT             <  SRR19973066.3178381/150‑1 (MQ=42)
                                                                                                                                         ATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAT             <  SRR19973066.3413945/150‑1 (MQ=42)
                                                                                                                                          TTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATG            <  SRR19973066.827803/150‑1 (MQ=42)
                                                                                                                                          TTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATG            <  SRR19973066.827234/150‑1 (MQ=42)
                                                                                                                                           TCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGA           >  SRR19973066.942061/1‑150 (MQ=42)
                                                                                                                                            CCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGAC          >  SRR19973066.658423/1‑150 (MQ=42)
                                                                                                                                             CTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACA         >  SRR19973066.2476172/1‑150 (MQ=42)
                                                                                                                                              TGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAA        <  SRR19973066.190196/150‑1 (MQ=42)
                                                                                                                                               GTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAG       <  SRR19973066.2103421/150‑1 (MQ=42)
                                                                                                                                               GTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAG       <  SRR19973066.2931053/150‑1 (MQ=42)
                                                                                                                                               GTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAG       <  SRR19973066.2931103/150‑1 (MQ=42)
                                                                                                                                                  CACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGT    <  SRR19973066.2654242/150‑1 (MQ=42)
                                                                                                                                                   ACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC   <  SRR19973066.420327/150‑1 (MQ=42)
                                                                                                                                                   ACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC   >  SRR19973066.3359906/1‑150 (MQ=42)
                                                                                                                                                   ACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTC   >  SRR19973066.352156/1‑150 (MQ=42)
                                                                                                                                                    CTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG  <  SRR19973066.815551/150‑1 (MQ=43)
                                                                                                                                                    CTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG  <  SRR19973066.815555/150‑1 (MQ=43)
                                                                                                                                                    CTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG  <  SRR19973066.815556/150‑1 (MQ=43)
                                                                                                                                                     |                                                                                                                                                    
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG  >  CP009273/732345‑732642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑