Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 732,494 G→T 90.3% pseudogene (214/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273732,4940GT90.3% 608.1 / 58.0 217pseudogene (214/1137 nt)ybfLpseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
Reads supporting (aligned to +/- strand):  ref base G (12/9);  new base T (93/101);  total (107/110)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 223 total aligned reads displayed.

GAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGT  >  CP009273/732347‑732640
                                                                                                                                                   |                                                                                                                                                  
gagCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGAt                                                                                                                                                  <  2:2563037/150‑1 (MQ=255)
gagCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGAt                                                                                                                                                  <  2:2562911/150‑1 (MQ=255)
 agCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACtata                                                                                                                                                 >  1:1234878/1‑150 (MQ=255)
 agCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACtata                                                                                                                                                 <  1:1828760/150‑1 (MQ=255)
  gCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATAc                                                                                                                                                >  1:2907842/1‑150 (MQ=255)
   cATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACt                                                                                                                                               <  1:963469/150‑1 (MQ=255)
    aTAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTa                                                                                                                                              >  2:1807113/1‑150 (MQ=255)
     tAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCactatactat                                                                                                                                             <  1:309168/150‑1 (MQ=255)
     tAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCactatactat                                                                                                                                             >  1:638538/1‑150 (MQ=255)
           gTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCag                                                                                                                                       >  2:2876626/1‑150 (MQ=255)
            tCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCaga                                                                                                                                      <  1:3225650/150‑1 (MQ=255)
             cAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCagag                                                                                                                                     <  2:4403820/150‑1 (MQ=255)
              aGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGt                                                                                                                                    >  1:3170495/1‑150 (MQ=255)
                   tCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTAt                                                                                                                               <  1:2572070/150‑1 (MQ=255)
                    ccTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATc                                                                                                                              <  1:780148/150‑1 (MQ=255)
                     ctactaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATcc                                                                                                                             >  2:1097359/1‑150 (MQ=255)
                        ctaTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGt                                                                                                                          >  2:2321524/1‑150 (MQ=255)
                         taTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTa                                                                                                                         <  1:3183447/150‑1 (MQ=255)
                         taTTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTa                                                                                                                         >  2:3019590/1‑150 (MQ=255)
                            tGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCa                                                                                                                      <  1:4129425/150‑1 (MQ=255)
                              aCTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGt                                                                                                                    >  1:4382644/1‑150 (MQ=255)
                                    tttGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCa                                                                                                              <  1:3937183/150‑1 (MQ=255)
                                      tGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCaaa                                                                                                            >  1:1758972/1‑150 (MQ=255)
                                        ccGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTATTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTTCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAt                                                                                                          >  2:1529585/1‑150 (MQ=255)
                                           ttATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTc                                                                                                       <  1:4036942/150‑1 (MQ=255)
                                            tATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCa                                                                                                      <  1:1213949/150‑1 (MQ=255)
                                              tttCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACg                                                                                                    <  1:196938/150‑1 (MQ=255)
                                                tCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAg                                                                                                  >  2:2017584/1‑150 (MQ=39)
                                                 cTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGt                                                                                                 >  1:4496920/1‑150 (MQ=255)
                                                 cTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGt                                                                                                 <  2:17712/150‑1 (MQ=255)
                                                    gTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCt                                                                                              >  1:109503/1‑150 (MQ=255)
                                                     tGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTATTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATAGACAGAGATGTATCATGTATCAGTCCTGAAAAATTTCAAGAGTGCtt                                                                                             >  1:3952744/1‑150 (MQ=11)
                                                       cAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTtta                                                                                           <  1:1535353/150‑1 (MQ=255)
                                                       cAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTtta                                                                                           >  1:669709/1‑150 (MQ=255)
                                                       cAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTtta                                                                                           >  1:4095617/1‑150 (MQ=39)
                                                             gTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACt                                                                                     <  2:1485996/150‑1 (MQ=37)
                                                             gTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACt                                                                                     <  2:1622127/150‑1 (MQ=37)
                                                              ttGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTg                                                                                    <  1:2250152/150‑1 (MQ=37)
                                                                gggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGa                                                                                  >  2:4421868/1‑150 (MQ=37)
                                                                gggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGa                                                                                  <  1:1540331/150‑1 (MQ=37)
                                                                gggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGa                                                                                  >  2:29933/1‑150 (MQ=37)
                                                                 ggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAt                                                                                 <  2:1257717/150‑1 (MQ=37)
                                                                 ggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAt                                                                                 >  2:4272179/1‑150 (MQ=37)
                                                                 ggAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAt                                                                                 <  1:2842376/150‑1 (MQ=255)
                                                                  gAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATg                                                                                <  2:267372/150‑1 (MQ=37)
                                                                   aaGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGc                                                                               <  1:2388237/150‑1 (MQ=37)
                                                                        ataGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAt                                                                          <  2:3069879/150‑1 (MQ=38)
                                                                        ataGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAt                                                                          <  2:3069856/150‑1 (MQ=38)
                                                                         taGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAtt                                                                         <  2:2454656/150‑1 (MQ=38)
                                                                         taGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAtt                                                                         <  1:2217217/150‑1 (MQ=38)
                                                                         taGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAtt                                                                         <  1:3086856/150‑1 (MQ=38)
                                                                             ggATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCa                                                                     >  1:1904314/1‑150 (MQ=255)
                                                                             ggATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCa                                                                     <  1:4254969/150‑1 (MQ=255)
                                                                              gATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAt                                                                    <  2:2642992/150‑1 (MQ=39)
                                                                              gATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAt                                                                    <  2:2643257/150‑1 (MQ=39)
                                                                              gATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAt                                                                    <  2:4091593/150‑1 (MQ=39)
                                                                               aTTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAtt                                                                   <  1:2295829/150‑1 (MQ=255)
                                                                               aTTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCAtt                                                                   >  2:712903/1‑150 (MQ=255)
                                                                                 ttCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtca                                                                 <  1:726557/150‑1 (MQ=255)
                                                                                   cGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTCTCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatc                                                               >  2:2096825/1‑150 (MQ=255)
                                                                                    ggggAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATtcatca                                                              >  2:4196150/1‑150 (MQ=255)
                                                                                       gAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAAt                                                           <  2:2652736/150‑1 (MQ=255)
                                                                                         aaCACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGa                                                         <  1:4206008/150‑1 (MQ=255)
                                                                                          acacATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAt                                                        <  1:742076/150‑1 (MQ=255)
                                                                                          acacATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAt                                                        >  1:4017453/1‑150 (MQ=255)
                                                                                             caTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATaaa                                                     >  1:383153/1‑150 (MQ=255)
                                                                                              aTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAg                                                    >  2:2095674/1‑150 (MQ=255)
                                                                                              aTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAg                                                    <  2:1211557/150‑1 (MQ=255)
                                                                                              aTCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAg                                                    <  2:4490608/150‑1 (MQ=255)
                                                                                                  cGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGt                                                >  1:2751655/1‑150 (MQ=255)
                                                                                                  cGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCATGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGt                                                >  1:1054020/1‑150 (MQ=255)
                                                                                                   gATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTc                                               >  1:3044215/1‑150 (MQ=255)
                                                                                                      ttCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCAtt                                            <  1:2844612/150‑1 (MQ=255)
                                                                                                        cTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGc                                          <  1:310261/150‑1 (MQ=255)
                                                                                                         ttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCa                                         <  2:9039/150‑1 (MQ=255)
                                                                                                         ttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCa                                         <  2:1686205/150‑1 (MQ=255)
                                                                                                            aaGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAtt                                      <  1:1351689/150‑1 (MQ=255)
                                                                                                            aaGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAtt                                      >  1:1253776/1‑150 (MQ=255)
                                                                                                            aaGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAAtt                                      <  2:3211028/150‑1 (MQ=255)
                                                                                                              gCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGa                                    <  1:80236/150‑1 (MQ=255)
                                                                                                                aaTATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATg                                  <  2:1078281/150‑1 (MQ=255)
                                                                                                                 atatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATgg                                 >  2:4597429/1‑150 (MQ=255)
                                                                                                                  tatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGCTTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGa                                <  2:4162843/150‑1 (MQ=255)
                                                                                                                  tatGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGa                                <  1:1886338/150‑1 (MQ=255)
                                                                                                                   atGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaa                               >  1:3382387/1‑150 (MQ=255)
                                                                                                                    tGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaa                              <  2:4019667/150‑1 (MQ=255)
                                                                                                                    tGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGaaa                              <  2:4019811/150‑1 (MQ=255)
                                                                                                                       tGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAc                           >  1:2890712/1‑150 (MQ=255)
                                                                                                                         aTTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGc                         >  2:1825383/1‑150 (MQ=255)
                                                                                                                           tttGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTc                       >  2:3295258/1‑150 (MQ=255)
                                                                                                                            ttGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTcc                      <  2:641289/150‑1 (MQ=255)
                                                                                                                             tGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTcca                     <  2:1373460/150‑2 (MQ=255)
                                                                                                                               aaaaTGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGc                   >  2:1847885/1‑150 (MQ=255)
                                                                                                                                      tATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa            >  1:3536541/1‑150 (MQ=255)
                                                                                                                                      tATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa            >  1:3369418/1‑150 (MQ=255)
                                                                                                                                      tATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGCGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTa            >  1:1648836/1‑150 (MQ=255)
                                                                                                                                       aTTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTAt           >  2:3934267/1‑150 (MQ=255)
                                                                                                                                        ttCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATg          <  2:3468827/150‑1 (MQ=255)
                                                                                                                                          ccTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGAc        >  1:3653060/1‑150 (MQ=39)
                                                                                                                                                cACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGt  >  2:2118923/1‑150 (MQ=39)
                                                                                                                                                   |                                                                                                                                                  
GAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGT  >  CP009273/732347‑732640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGC  >  CP009273/732345‑732643
                                                                                                                                                     |                                                                                                                                                     
TAGAGAATAATTTGTCAGGGATCCTACTACTGTCTATATTTGGCTTTATTTCTGGTGCAGAATGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACT                                                                                                                                                       <  SRR19973073.480607/150‑1 (MQ=17)
 AGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTA                                                                                                                                                      <  SRR19973073.3053775/150‑1 (MQ=60)
 AGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTA                                                                                                                                                      >  SRR19973073.1473094/1‑150 (MQ=60)
   AGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATA                                                                                                                                                    >  SRR19973073.1237460/1‑150 (MQ=60)
   AGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATA                                                                                                                                                    >  SRR19973073.1237486/1‑150 (MQ=60)
   AGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATA                                                                                                                                                    <  SRR19973073.1832534/150‑1 (MQ=60)
    GCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATAC                                                                                                                                                   >  SRR19973073.2913783/1‑150 (MQ=60)
    GCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATAC                                                                                                                                                   >  SRR19973073.2913767/1‑150 (MQ=60)
     CATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACT                                                                                                                                                  <  SRR19973073.965483/150‑1 (MQ=60)
       TAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTTGTGCAGAAAGTTGGGAAGATTTAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTAT                                                                                                                                                <  SRR19973073.310141/150‑1 (MQ=60)
       TAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTAT                                                                                                                                                <  SRR19973073.309813/150‑1 (MQ=60)
       TAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTAT                                                                                                                                                >  SRR19973073.639898/1‑150 (MQ=60)
         AATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTG                                                                                                                                              >  SRR19973073.1279975/1‑150 (MQ=60)
              TCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGA                                                                                                                                         <  SRR19973073.3232156/150‑1 (MQ=60)
                AGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGT                                                                                                                                       >  SRR19973073.3176891/1‑150 (MQ=60)
                     TCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTAT                                                                                                                                  <  SRR19973073.2577371/150‑1 (MQ=57)
                     TCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTAT                                                                                                                                  <  SRR19973073.2577418/150‑1 (MQ=57)
                      CCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATC                                                                                                                                 <  SRR19973073.781727/150‑1 (MQ=55)
                        TACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCT                                                                                                                               <  SRR19973073.2539272/150‑1 (MQ=55)
                          CTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGT                                                                                                                             >  SRR19973073.4258695/1‑150 (MQ=55)
                          CTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGT                                                                                                                             >  SRR19973073.3214474/1‑150 (MQ=55)
                           TATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTA                                                                                                                            <  SRR19973073.3189870/150‑1 (MQ=55)
                              TGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCA                                                                                                                         <  SRR19973073.1489072/150‑1 (MQ=57)
                              TGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCA                                                                                                                         <  SRR19973073.4137687/150‑1 (MQ=57)
                                ACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGT                                                                                                                       >  SRR19973073.4391473/1‑150 (MQ=60)
                                ACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGT                                                                                                                       >  SRR19973073.4391435/1‑150 (MQ=60)
                                      TTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCATAGTTGTATCCTGTATCAGTCCTGCA                                                                                                                 >  SRR19973073.4268561/1‑150 (MQ=60)
                                      TTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCA                                                                                                                 >  SRR19973073.2431033/1‑150 (MQ=60)
                                      TTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCA                                                                                                                 <  SRR19973073.3945029/150‑1 (MQ=60)
                                       TTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAA                                                                                                                <  SRR19973073.1582020/150‑1 (MQ=45)
                                        TGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAA                                                                                                               >  SRR19973073.1762613/1‑150 (MQ=42)
                                          CCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAT                                                                                                             <  SRR19973073.1141309/150‑1 (MQ=60)
                                          CCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAAT                                                                                                             >  SRR19973073.680464/1‑150 (MQ=42)
                                           CGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATT                                                                                                            >  SRR19973073.1962939/1‑150 (MQ=42)
                                             TTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTC                                                                                                          <  SRR19973073.4045014/150‑1 (MQ=60)
                                              TATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCA                                                                                                         <  SRR19973073.1216475/150‑1 (MQ=60)
                                               ATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCAC                                                                                                        <  SRR19973073.107178/150‑1 (MQ=60)
                                                TTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACG                                                                                                       <  SRR19973073.197419/150‑1 (MQ=42)
                                                TTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACG                                                                                                       <  SRR19973073.197396/150‑1 (MQ=42)
                                                TTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACG                                                                                                       >  SRR19973073.1754228/1‑150 (MQ=42)
                                                   CTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGT                                                                                                    >  SRR19973073.4505959/1‑150 (MQ=42)
                                                   CTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATCTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGT                                                                                                    >  SRR19973073.3157585/1‑150 (MQ=41)
                                                     GGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGC                                                                                                  <  SRR19973073.123429/150‑1 (MQ=42)
                                                      GTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCT                                                                                                 >  SRR19973073.109773/1‑150 (MQ=42)
                                                       TGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTATTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATAGACAGAGATGTATCATGTATCAGTCCTGAAAAATTTCAAGAGTGCTT                                                                                                >  SRR19973073.3960613/1‑150 (MQ=45)
                                                         CAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTA                                                                                              <  SRR19973073.1538496/150‑1 (MQ=42)
                                                         CAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTA                                                                                              <  SRR19973073.1538517/150‑1 (MQ=42)
                                                         CAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTA                                                                                              >  SRR19973073.671104/1‑150 (MQ=42)
                                                         CAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTA                                                                                              >  SRR19973073.4103808/1‑150 (MQ=43)
                                                           GAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATT                                                                                            <  SRR19973073.2241150/150‑1 (MQ=40)
                                                            AAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTA                                                                                           <  SRR19973073.3622374/150‑1 (MQ=38)
                                                            AAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTA                                                                                           >  SRR19973073.2101726/1‑150 (MQ=38)
                                                             AAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAA                                                                                          <  SRR19973073.4191769/150‑1 (MQ=36)
                                                                TTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTG                                                                                       <  SRR19973073.2254778/150‑1 (MQ=32)
                                                                  GGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGA                                                                                     <  SRR19973073.1543479/150‑1 (MQ=32)
                                                                   GGAAATTATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAT                                                                                    <  SRR19973073.2848191/150‑1 (MQ=31)
                                                                   GGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAT                                                                                    <  SRR19973073.2848205/150‑1 (MQ=31)
                                                                   GGAAAATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGAT                                                                                    <  SRR19973073.531316/150‑1 (MQ=31)
                                                                     AAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGC                                                                                  <  SRR19973073.2393133/150‑1 (MQ=32)
                                                                         TATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGA                                                                              >  SRR19973073.1776956/1‑150 (MQ=32)
                                                                           TAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATT                                                                            <  SRR19973073.3093103/150‑1 (MQ=36)
                                                                           TAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATT                                                                            <  SRR19973073.2221811/150‑1 (MQ=36)
                                                                           TAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATT                                                                            <  SRR19973073.3093053/150‑1 (MQ=36)
                                                                               GGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCA                                                                        <  SRR19973073.4263386/150‑1 (MQ=42)
                                                                               GGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCA                                                                        >  SRR19973073.1908191/1‑150 (MQ=42)
                                                                                 ATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATT                                                                      <  SRR19973073.2300511/150‑1 (MQ=42)
                                                                                   TTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCA                                                                    <  SRR19973073.728001/150‑1 (MQ=40)
                                                                                   TTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCA                                                                    <  SRR19973073.728049/150‑1 (MQ=40)
                                                                                   TTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCA                                                                    <  SRR19973073.65124/150‑1 (MQ=40)
                                                                                      GGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCA                                                                 >  SRR19973073.3453056/1‑150 (MQ=40)
                                                                                      GGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCA                                                                 >  SRR19973073.3753659/1‑150 (MQ=40)
                                                                                        GGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAA                                                               >  SRR19973073.403241/1‑150 (MQ=44)
                                                                                           AACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGA                                                            <  SRR19973073.4214358/150‑1 (MQ=50)
                                                                                            ACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAT                                                           <  SRR19973073.743584/150‑1 (MQ=53)
                                                                                            ACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAT                                                           >  SRR19973073.4025482/1‑150 (MQ=53)
                                                                                            ACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAT                                                           <  SRR19973073.743129/150‑1 (MQ=53)
                                                                                            ACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGAT                                                           >  SRR19973073.4025177/1‑150 (MQ=53)
                                                                                              ACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAA                                                         <  SRR19973073.1673549/150‑1 (MQ=53)
                                                                                              ACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAA                                                         >  SRR19973073.3953819/1‑150 (MQ=53)
                                                                                              ACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAA                                                         <  SRR19973073.4090223/150‑1 (MQ=53)
                                                                                               CATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAA                                                        >  SRR19973073.383899/1‑150 (MQ=53)
                                                                                               CATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAA                                                        >  SRR19973073.383906/1‑150 (MQ=53)
                                                                                                   TCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACG                                                    <  SRR19973073.2273082/150‑1 (MQ=53)
                                                                                                    CGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGT                                                   >  SRR19973073.2757292/1‑150 (MQ=53)
                                                                                                    CGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCATGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGT                                                   >  SRR19973073.1056212/1‑150 (MQ=51)
                                                                                                     GATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTC                                                  >  SRR19973073.3050367/1‑150 (MQ=53)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATT                                               <  SRR19973073.1853057/150‑1 (MQ=48)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATT                                               <  SRR19973073.2850420/150‑1 (MQ=48)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATT                                               <  SRR19973073.2850443/150‑1 (MQ=48)
                                                                                                        TTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATT                                               <  SRR19973073.2850444/150‑1 (MQ=48)
                                                                                                          CTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGC                                             <  SRR19973073.310804/150‑1 (MQ=44)
                                                                                                          CTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGC                                             <  SRR19973073.310906/150‑1 (MQ=44)
                                                                                                              AAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATT                                         <  SRR19973073.1354559/150‑1 (MQ=42)
                                                                                                              AAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATT                                         >  SRR19973073.3867616/1‑150 (MQ=42)
                                                                                                              AAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATT                                         >  SRR19973073.1256398/1‑150 (MQ=42)
                                                                                                                GCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGA                                       <  SRR19973073.80452/150‑1 (MQ=42)
                                                                                                                 CAATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGAT                                      <  SRR19973073.431186/150‑1 (MQ=42)
                                                                                                                   ATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGG                                    >  SRR19973073.135329/1‑150 (MQ=42)
                                                                                                                   ATATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGG                                    >  SRR19973073.3091770/1‑150 (MQ=42)
                                                                                                                    TATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGA                                   <  SRR19973073.1890193/150‑1 (MQ=42)
                                                                                                                    TATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGA                                   <  SRR19973073.1890276/150‑1 (MQ=42)
                                                                                                                     ATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAA                                  >  SRR19973073.746322/1‑150 (MQ=42)
                                                                                                                     ATGGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAA                                  >  SRR19973073.3389188/1‑150 (MQ=42)
                                                                                                                       GGTGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAA                                >  SRR19973073.3263030/1‑150 (MQ=42)
                                                                                                                         TGATTTTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAAC                              >  SRR19973073.2896617/1‑150 (MQ=42)
                                                                                                                              TTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCC                         >  SRR19973073.944143/1‑150 (MQ=42)
                                                                                                                              TTGAAAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCC                         >  SRR19973073.781832/1‑150 (MQ=42)
                                                                                                                                  AAATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTAACGAGTGCTTTATTAACTGGATGCGTGATTGCAATTCATCAAATTATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCA                     >  SRR19973073.3386651/1‑150 (MQ=39)
                                                                                                                                    ATGGTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGAAAAATTTCTCGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACT                   <  SRR19973073.4068000/150‑1 (MQ=40)
                                                                                                                                       GTATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTT                <  SRR19973073.412564/150‑1 (MQ=42)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGCGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA               <  SRR19973073.3039426/150‑1 (MQ=41)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA               >  SRR19973073.3376212/1‑150 (MQ=42)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA               >  SRR19973073.3376171/1‑150 (MQ=42)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA               >  SRR19973073.3543621/1‑150 (MQ=42)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA               >  SRR19973073.286681/1‑150 (MQ=42)
                                                                                                                                        TATTCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGCGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTA               >  SRR19973073.1652253/1‑150 (MQ=41)
                                                                                                                                           TCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGA            <  SRR19973073.2332284/150‑1 (MQ=42)
                                                                                                                                           TCCTGTTCACTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGA            <  SRR19973073.3183513/150‑1 (MQ=42)
                                                                                                                                            CCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGAC           >  SRR19973073.3660394/1‑150 (MQ=43)
                                                                                                                                                    CTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG   >  SRR19973073.3709505/1‑150 (MQ=43)
                                                                                                                                                    CTATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCG   >  SRR19973073.3709481/1‑150 (MQ=43)
                                                                                                                                                     TATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGC  >  SRR19973073.2727314/1‑150 (MQ=43)
                                                                                                                                                     TATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGC  <  SRR19973073.2967967/150‑1 (MQ=43)
                                                                                                                                                     |                                                                                                                                                     
TAGAGCATAAATTGTCAGGCATCCTACTATTGACTATTTTTGCCGTTATTTCTGGTGCAGAAAGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTCTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACTATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGC  >  CP009273/732345‑732643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑