Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 738,124:1 +T 12.8% intergenic (‑112/‑159) dtpD ← / → ybgI dipeptide and tripeptide permease D/NIF3 family metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273738,1241.T12.8% 1009.5 / 165.8 273intergenic (‑112/‑159)dtpD/ybgIdipeptide and tripeptide permease D/NIF3 family metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (110/128);  new base T (24/11);  total (134/139)
Fisher's exact test for biased strand distribution p-value = 1.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 286 total aligned reads displayed.

TAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAA  >  CP009273/737975‑738273
                                                                                                                                                      |                                                                                                                                                     
tAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGa                                                                                                                                                        <  2:1260722/150‑1 (MQ=255)
  gTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑tt                                                                                                                                                     >  1:4122800/1‑150 (MQ=255)
           gcgcgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAata                                                                                                                                            <  1:1089567/150‑1 (MQ=255)
              cgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATAc                                                                                                                                         <  2:2295483/150‑1 (MQ=255)
               ggCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACa                                                                                                                                        >  2:501985/1‑150 (MQ=255)
                 cTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACAtt                                                                                                                                      <  2:568017/150‑1 (MQ=255)
                  tgtgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTa                                                                                                                                     >  1:888160/1‑150 (MQ=255)
                    tgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGc                                                                                                                                   >  1:1261149/1‑150 (MQ=255)
                     gATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCt                                                                                                                                  <  1:3222923/150‑1 (MQ=255)
                         cGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAg                                                                                                                              >  1:2867567/1‑150 (MQ=255)
                           tgtTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCg                                                                                                                            <  1:20376/150‑1 (MQ=255)
                            gtTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTACTAGTTGAGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGt                                                                                                                           >  1:2570778/1‑150 (MQ=255)
                            gtTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGt                                                                                                                           <  1:3915131/150‑1 (MQ=255)
                               tATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGtgat                                                                                                                        >  2:3488522/1‑150 (MQ=255)
                               tATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGtgat                                                                                                                        <  2:1727717/150‑1 (MQ=255)
                                aTTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGtgatg                                                                                                                       <  1:1957329/150‑1 (MQ=255)
                                 ttCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGtgatga                                                                                                                      >  2:2997787/1‑150 (MQ=255)
                                     taataaTTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGatat                                                                                                                   >  1:737754/1‑150 (MQ=255)
                                     taataaTTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGAtata                                                                                                                  >  1:4203381/1‑150 (MQ=255)
                                      aataatTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATaa                                                                                                                 >  1:4000320/1‑150 (MQ=255)
                                         aatTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACa                                                                                                              >  1:2162584/1‑150 (MQ=255)
                                            tCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAAt                                                                                                            >  1:1425428/1‑150 (MQ=255)
                                              cacaATTGACGGCAACGTCACGTAATAGTTGCGCTGCGTGCTTTTGTTTACTTAAAGTTAAAACTTTGATACATATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATGAGCTGAAGCGTGATGATATAAACAATTTg                                                                                                         <  2:211480/150‑1 (MQ=255)
                                               acaATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGc                                                                                                        >  1:61146/1‑150 (MQ=255)
                                                caATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCg                                                                                                       >  1:244812/1‑150 (MQ=255)
                                                  aTTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTc                                                                                                     >  1:2958738/1‑150 (MQ=255)
                                                     gACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCttt                                                                                                  <  1:3035012/150‑1 (MQ=255)
                                                      aCGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCtttt                                                                                                 <  2:889916/150‑1 (MQ=255)
                                                       cGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg                                                                                                <  2:1798307/150‑1 (MQ=255)
                                                       cGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg                                                                                                <  1:3996268/150‑1 (MQ=255)
                                                       cGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg                                                                                                >  1:1688063/1‑150 (MQ=255)
                                                       cGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg                                                                                                <  1:2499156/150‑1 (MQ=255)
                                                       cGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg                                                                                                <  1:1441989/150‑1 (MQ=255)
                                                        ggCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTgg                                                                                               <  1:2631767/150‑1 (MQ=255)
                                                          cAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCg                                                                                             <  1:3514086/150‑1 (MQ=255)
                                                           aaCGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCg                                                                                             >  2:2453420/1‑150 (MQ=255)
                                                           aaCGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGt                                                                                            <  2:1396228/150‑1 (MQ=255)
                                                             cGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCg                                                                                          >  2:3556135/1‑150 (MQ=255)
                                                             cGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCg                                                                                          <  1:2543582/150‑1 (MQ=255)
                                                             cGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCg                                                                                          >  2:3094165/1‑150 (MQ=255)
                                                             cGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCg                                                                                          >  2:3431461/1‑150 (MQ=255)
                                                              gTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCgg                                                                                         >  1:93254/1‑150 (MQ=255)
                                                               tCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCgg                                                                                         >  2:4231280/1‑150 (MQ=255)
                                                                cACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgt                                                                                       <  1:1328418/150‑1 (MQ=255)
                                                                 aCGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtt                                                                                      <  2:4092826/150‑1 (MQ=255)
                                                                 aCGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtt                                                                                      <  1:71241/150‑1 (MQ=255)
                                                                   gTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtttg                                                                                    <  1:2688971/150‑1 (MQ=255)
                                                                        aGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCt                                                                               >  2:3966083/1‑150 (MQ=255)
                                                                         gTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCtt                                                                              <  2:4036541/150‑1 (MQ=255)
                                                                         gTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCtt                                                                              <  1:1996346/150‑1 (MQ=255)
                                                                                ggcgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGt                                                                       >  1:4382467/1‑150 (MQ=255)
                                                                                  cgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTc                                                                      <  2:2806401/150‑1 (MQ=255)
                                                                                  cgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTc                                                                      >  2:198967/1‑150 (MQ=255)
                                                                                  cgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCa                                                                     <  1:2321701/150‑1 (MQ=255)
                                                                                  cgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCa                                                                     >  1:2338240/1‑150 (MQ=255)
                                                                                        tgtgTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGAt                                                               <  2:2511998/150‑1 (MQ=255)
                                                                                            tttCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTgg                                                            <  2:3711815/150‑1 (MQ=255)
                                                                                            tttCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGc                                                           >  2:1544470/1‑150 (MQ=255)
                                                                                                cTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATc                                                       >  2:2159139/1‑150 (MQ=255)
                                                                                                 ttAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcg                                                      <  2:930364/150‑1 (MQ=255)
                                                                                                  tAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcga                                                     >  1:199491/1‑150 (MQ=255)
                                                                                                     aGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcgacga                                                  <  2:1311426/150‑1 (MQ=255)
                                                                                                      gTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGaa                                                 >  2:805942/1‑150 (MQ=255)
                                                                                                         aaaaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTg                                              >  1:2098793/1‑150 (MQ=255)
                                                                                                         aaaaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTg                                              >  2:4442794/1‑150 (MQ=255)
                                                                                                           aaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGc                                            <  1:2590905/150‑1 (MQ=255)
                                                                                                           aaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGc                                            <  1:4553822/150‑1 (MQ=255)
                                                                                                           aaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGc                                            >  1:2180372/1‑150 (MQ=255)
                                                                                                           aaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGc                                            >  1:3704742/1‑150 (MQ=255)
                                                                                                            aCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCg                                           <  2:3601021/150‑1 (MQ=255)
                                                                                                            aCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCg                                           <  1:3830328/150‑1 (MQ=255)
                                                                                                             cTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCg                                           >  1:829157/1‑150 (MQ=255)
                                                                                                              tttGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAg                                         <  1:1007589/150‑1 (MQ=255)
                                                                                                                      aGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCt                                 <  2:1619865/150‑1 (MQ=255)
                                                                                                                      aGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCt                                 >  1:1583620/1‑150 (MQ=255)
                                                                                                                      aGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCt                                 <  2:748901/150‑1 (MQ=255)
                                                                                                                       gATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTa                                >  2:1307994/1‑150 (MQ=255)
                                                                                                                       gATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTa                                <  2:3264724/150‑1 (MQ=255)
                                                                                                                         tCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATg                              <  2:4397499/150‑1 (MQ=255)
                                                                                                                           tGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGtt                            <  2:4140623/150‑1 (MQ=255)
                                                                                                                           tGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGtt                            >  1:3157721/1‑150 (MQ=255)
                                                                                                                            gATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTa                           >  1:2784902/1‑150 (MQ=255)
                                                                                                                              ttatttatGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTaa                          >  1:1375844/1‑150 (MQ=255)
                                                                                                                              ttatttatGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAAc                         >  1:2102983/1‑150 (MQ=255)
                                                                                                                                atttatGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACgg                       >  1:1553780/1‑150 (MQ=255)
                                                                                                                                 tttatGCAATATGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACgg                       <  1:1521327/150‑1 (MQ=255)
                                                                                                                                 tttatGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGgt                      <  2:3719815/150‑1 (MQ=255)
                                                                                                                                 tttatGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGgt                      <  2:3116636/150‑1 (MQ=255)
                                                                                                                                   tatGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGgtgt                    >  2:447524/1‑150 (MQ=255)
                                                                                                                                   tatGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGgtgt                    <  2:2339075/150‑1 (MQ=255)
                                                                                                                                    atGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTa                   >  1:1934659/1‑150 (MQ=255)
                                                                                                                                        aaTATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTa               <  2:674404/150‑1 (MQ=255)
                                                                                                                                         atatGCTGTCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTa               >  2:2374149/1‑150 (MQ=255)
                                                                                                                                         atatGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTAt              >  1:3474990/1‑150 (MQ=255)
                                                                                                                                          tatGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATc             <  1:182144/150‑1 (MQ=255)
                                                                                                                                           atGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCa            <  2:1083057/150‑1 (MQ=255)
                                                                                                                                                 tCTGATTTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATa       >  2:2655362/1‑150 (MQ=255)
                                                                                                                                                    gA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGa   <  1:2816621/150‑1 (MQ=255)
                                                                                                                                                     a‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGaa  >  2:1336249/1‑150 (MQ=255)
                                                                                                                                                     a‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGaa  >  2:2085372/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑TTGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAA  >  CP009273/737975‑738273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A