Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	738,124:1	+T	46.0%	intergenic (‑112/‑159)	dtpD ← / → ybgI	dipeptide and tripeptide permease D/NIF3 family metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	738,124	1	.	T	46.0%	inf / inf	226	intergenic (‑112/‑159)	dtpD/ybgI	dipeptide and tripeptide permease D/NIF3 family metal‑binding protein
Reads supporting (aligned to +/- strand): ref base . (58/64); new base T (49/55); total (107/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 238 total aligned reads displayed.

AGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGA  >  CP009273/737976‑738272

aGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t                                                                                                                                                      <  1:1005785/150‑1 (MQ=255)

aGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t                                                                                                                                                      >  2:932801/1‑150 (MQ=255)

aGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t                                                                                                                                                      >  2:4359120/1‑150 (MQ=255)

 gTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t‑t                                                                                                                                                    <  2:1267818/150‑1 (MQ=255)

  tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t‑tt                                                                                                                                                   <  2:1571435/150‑2 (MQ=255)

  tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑Tg                                                                                                                                                   >  2:400911/1‑150 (MQ=255)

  tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑Tg                                                                                                                                                   <  1:2201580/150‑1 (MQ=255)

      tAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t‑ttgcat                                                                                                                                               <  2:3357847/150‑6 (MQ=255)

      tAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑t‑ttgcat                                                                                                                                               >  1:919019/1‑145 (MQ=255)

          gcgcgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAat                                                                                                                                            <  2:1373047/150‑1 (MQ=255)

           cgcgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAata                                                                                                                                           <  2:4242536/150‑1 (MQ=255)

           cgcgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAata                                                                                                                                           <  2:2449902/150‑1 (MQ=255)

             cgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATAc                                                                                                                                        >  1:985295/1‑150 (MQ=255)

             cgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATAc                                                                                                                                        >  1:470747/1‑150 (MQ=255)

             cgGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATAc                                                                                                                                        <  1:3400837/150‑1 (MQ=255)

               gCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACa                                                                                                                                       <  1:1053192/150‑1 (MQ=255)

                 tgtgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACAtt                                                                                                                                     >  1:2406018/1‑150 (MQ=255)

                 tgtgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTa                                                                                                                                    <  2:3232950/150‑1 (MQ=255)

                  gtgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTa                                                                                                                                    >  2:4598837/1‑150 (MQ=255)

                  gtgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAg                                                                                                                                   <  1:345201/150‑1 (MQ=255)

                   tgATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAg                                                                                                                                   >  2:2246749/1‑150 (MQ=255)

                    gATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTAGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTAATGCAATATGCTGTCTGA‑TATGCATAAATATACATTAGc                                                                                                                                  >  1:222356/1‑150 (MQ=255)

                     aggCGTGTTTATTCATAATAAGGCCACAATTGACGGCATCGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCt                                                                                                                                 <  1:3187721/148‑1 (MQ=255)

                      tGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTg                                                                                                                                >  2:869786/1‑150 (MQ=255)

                      tGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTg                                                                                                                                >  2:4360203/1‑150 (MQ=255)

                        cGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGaa                                                                                                                              >  1:3932541/1‑150 (MQ=255)

                        cGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTAATGCAATATGCTGTCTGA‑T‑TGCATAAATATAAATTAGCTGAAg                                                                                                                             >  1:4187705/1‑150 (MQ=255)

                          tgtTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGc                                                                                                                            <  2:2936698/150‑1 (MQ=255)

                           gtTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCg                                                                                                                           >  2:4210142/1‑150 (MQ=255)

                               aTTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGtgatg                                                                                                                      <  1:664072/150‑1 (MQ=255)

                               aTTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGtgatg                                                                                                                      <  1:2287229/150‑1 (MQ=255)

                                    taataaTTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGAtata                                                                                                                 >  1:326807/1‑150 (MQ=255)

                                     aataatTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGAtata                                                                                                                 <  2:1547096/150‑1 (MQ=255)

                                        aatTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACa                                                                                                             >  1:2070468/1‑150 (MQ=255)

                                            ccACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAAtt                                                                                                          >  2:4469382/1‑150 (MQ=255)

                                            ccACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAAtt                                                                                                          <  1:2838327/150‑1 (MQ=255)

                                            ccACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAAttt                                                                                                         <  2:4531412/150‑1 (MQ=255)

                                                aaTTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCg                                                                                                      >  2:2409907/1‑150 (MQ=255)

                                                    gACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCtt                                                                                                  >  1:3757430/1‑150 (MQ=255)

                                                    gACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCttt                                                                                                 <  1:643873/150‑1 (MQ=255)

                                                     aCGGCAACGTCACGTTATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCttt                                                                                                 <  2:1669532/150‑1 (MQ=255)

                                                      cGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCtttt                                                                                                <  2:853352/150‑1 (MQ=255)

                                                       ggCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTg                                                                                               <  2:1115444/150‑1 (MQ=255)

                                                         cAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGc                                                                                             <  2:571651/150‑1 (MQ=255)

                                                         cAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCg                                                                                            >  1:820619/1‑150 (MQ=255)

                                                             gTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCg                                                                                         <  1:4456085/150‑1 (MQ=255)

                                                               cACGTAATAGTTGCGCGGCGTTCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCggg                                                                                       <  2:3560354/150‑1 (MQ=255)

                                                               cACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCggg                                                                                       <  1:3652099/150‑1 (MQ=255)

                                                               cACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCggg                                                                                       <  2:2583914/150‑1 (MQ=255)

                                                                aCGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtt                                                                                     <  1:3727226/150‑1 (MQ=255)

                                                                 cGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgttt                                                                                    <  2:1907664/150‑1 (MQ=255)

                                                                 cGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAATTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAACGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgttt                                                                                    <  2:3071911/150‑1 (MQ=255)

                                                                  gTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtttg                                                                                   <  1:1333778/150‑1 (MQ=255)

                                                                   tAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtttg                                                                                   >  1:3577895/1‑150 (MQ=255)

                                                                   tAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtttg                                                                                   <  2:2075353/150‑1 (MQ=255)

                                                                    aaTAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtttgt                                                                                  <  2:1973597/150‑1 (MQ=255)

                                                                    aaTAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGgtttgtt                                                                                 >  1:198517/1‑150 (MQ=255)

                                                                       aGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCt                                                                              >  1:4383637/1‑150 (MQ=255)

                                                                          tGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTa                                                                            <  1:1837061/150‑1 (MQ=255)

                                                                          tGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTAGGGTTTGTTTCTTaa                                                                           <  1:4164921/150‑1 (MQ=255)

                                                                               ggcgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATAAATTAGCTGACGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGt                                                                      >  2:2282277/1‑150 (MQ=255)

                                                                                 cgTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCa                                                                    >  1:1887518/1‑150 (MQ=255)

                                                                                     cTTGTGTTTCCTTAAATTTAACACTTTGATACATTTCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATTAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAg                                                                <  1:2703164/150‑1 (MQ=255)

                                                                                      ttGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAg                                                                <  1:1632355/150‑1 (MQ=255)

                                                                                         tgtTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTg                                                            >  2:3916405/1‑150 (MQ=255)

                                                                                           tttCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGc                                                          <  2:3829165/150‑1 (MQ=255)

                                                                                            ttCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCt                                                         <  2:4029670/150‑1 (MQ=255)

                                                                                             tCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCt                                                         <  2:4067120/150‑1 (MQ=255)

                                                                                               cTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTAt                                                       >  2:2009898/1‑150 (MQ=255)

                                                                                               cTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTAt                                                       >  1:3814854/1‑150 (MQ=255)

                                                                                                ttAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcg                                                     >  2:4055101/1‑150 (MQ=255)

                                                                                                   aaGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcgac                                                   >  2:2916683/1‑150 (MQ=255)

                                                                                                   aaGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcgac                                                   <  1:2655586/150‑1 (MQ=255)

                                                                                                   aaGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcgacg                                                  >  2:4299522/1‑150 (MQ=255)

                                                                                                    aGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATcgacg                                                  <  2:2678509/150‑1 (MQ=255)

                                                                                                     gTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGaa                                                <  1:3875169/150‑1 (MQ=255)

                                                                                                       tAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAAc                                               >  1:1186885/1‑150 (MQ=255)

                                                                                                        aaaaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTg                                             >  2:3409392/1‑150 (MQ=255)

                                                                                                         aaaCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTgg                                            <  1:1534887/150‑1 (MQ=255)

                                                                                                           aCTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCg                                          <  1:788819/150‑1 (MQ=255)

                                                                                                            cTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCg                                          >  1:3762999/1‑150 (MQ=255)

                                                                                                             tttGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAg                                        >  2:2187050/1‑150 (MQ=255)

                                                                                                                gATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTa                                     >  1:290648/1‑150 (MQ=255)

                                                                                                                   aCAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACg                                  <  2:238171/150‑1 (MQ=255)

                                                                                                                    cAGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACg                                  >  2:3485366/1‑150 (MQ=255)

                                                                                                                     aGATCTGATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGc                                 >  1:4528496/1‑150 (MQ=255)

                                                                                                                      gATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTa                               >  2:3815781/1‑150 (MQ=255)

                                                                                                                           gATTATTTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGtt                           >  1:2912245/1‑150 (MQ=255)

                                                                                                                            attattTATGCAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTa                          >  1:3152027/1‑150 (MQ=255)

                                                                                                                             ttatttatGTAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTaa                         <  1:173851/150‑1 (MQ=255)

                                                                                                                                   atGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTa                  <  1:1863746/150‑1 (MQ=255)

                                                                                                                                      cAATATGCTGTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTCTGTTAACGGTGTAGc                >  2:3779941/1‑150 (MQ=255)

                                                                                                                                           tGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAg          >  2:4145452/1‑150 (MQ=255)

                                                                                                                                            gCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGa         <  2:2009704/150‑1 (MQ=255)

                                                                                                                                             cTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGaa        <  2:3131665/150‑1 (MQ=255)

                                                                                                                                               gTCTGATT‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAAt       <  2:795305/150‑1 (MQ=255)

                                                                                                                                               gTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATa      <  2:2962671/150‑1 (MQ=255)

                                                                                                                                                tCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATAc     <  2:1392175/150‑1 (MQ=255)

                                                                                                                                                   gA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGa  >  2:679359/1‑150 (MQ=255)

                                                                                                                                                   gA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGa  >  2:930914/1‑150 (MQ=255)

AGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTTGCGCGGCGTGCTTGTGTTTCCTTAAAGTTAAAACTTTGATACAGATCTGATTATTTATGCAATATGCTGTCTGA‑T‑TGCATAAATATACATTAGCTGAAGCGTGATGATATAAACAATTTGCGTCTTTTGGCGTCGGGTTTGTTTCTTAATATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGA  >  CP009273/737976‑738272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A