Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,314,870 C→A 100% A35S (GCG→TCG)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,314,8700CA97.4% 727.6 / 7.6 224A35S (GCG→TCG) yhbXputative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (93/124);  total (95/129)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAAT  >  CP009273/3314723‑3315016
                                                                                                                                                   |                                                                                                                                                  
tACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATc                                                                                                                                                  >  2:185817/1‑150 (MQ=255)
 acacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATcc                                                                                                                                                 <  2:4458286/150‑1 (MQ=255)
 acacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATcc                                                                                                                                                 >  1:1844936/1‑150 (MQ=255)
  cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCg                                                                                                                                                <  1:401615/150‑1 (MQ=255)
     cATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaa                                                                                                                                             >  1:2884727/1‑150 (MQ=255)
      aTGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaag                                                                                                                                            <  1:2092634/150‑1 (MQ=255)
        gAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaagaa                                                                                                                                          <  1:3727348/150‑1 (MQ=255)
         aaTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGaagaag                                                                                                                                         >  2:3504279/1‑150 (MQ=255)
          aTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGc                                                                                                                                        <  2:2030884/150‑1 (MQ=255)
               ggCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTa                                                                                                                                   <  2:899169/150‑1 (MQ=255)
               ggCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTa                                                                                                                                   >  2:197932/1‑150 (MQ=255)
                  tAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGt                                                                                                                                >  2:2831698/1‑150 (MQ=255)
                  tAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGt                                                                                                                                >  2:1186110/1‑150 (MQ=255)
                   aaTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGtt                                                                                                                               <  1:2994608/150‑1 (MQ=255)
                     tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCg                                                                                                                             >  2:2180171/1‑150 (MQ=255)
                      aCTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGt                                                                                                                            >  1:4079512/1‑150 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                         <  2:1726002/150‑1 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                         <  1:3887170/150‑1 (MQ=255)
                            gTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATAcc                                                                                                                      >  1:2694709/1‑150 (MQ=255)
                               tGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCaaa                                                                                                                   >  2:3881648/1‑150 (MQ=255)
                                 ccGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCaaaaa                                                                                                                 <  1:1018664/150‑1 (MQ=255)
                                   gCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACa                                                                                                               >  2:3971917/1‑150 (MQ=255)
                                        cagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAac                                                                                                          >  1:3186031/1‑150 (MQ=255)
                                        cagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAac                                                                                                          >  1:1034015/1‑150 (MQ=255)
                                        cagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAac                                                                                                          >  1:1035070/1‑150 (MQ=255)
                                           tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGTAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAcaca                                                                                                       <  2:4350225/150‑1 (MQ=255)
                                               acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATGTACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGa                                                                                                   >  1:3773168/1‑150 (MQ=255)
                                                caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGAt                                                                                                  <  2:4206119/150‑1 (MQ=255)
                                                  aaTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATac                                                                                                <  1:4176501/150‑1 (MQ=255)
                                                      gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATacaaca                                                                                            <  2:2239728/150‑1 (MQ=255)
                                                      gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATacaaca                                                                                            >  1:3426142/1‑150 (MQ=255)
                                                        gCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACaacaac                                                                                          <  1:1912768/150‑1 (MQ=255)
                                                         cGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAAcc                                                                                         >  2:3364759/1‑150 (MQ=255)
                                                          ggCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCa                                                                                        <  1:2054907/150‑1 (MQ=255)
                                                          ggCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTCCGTAATACCAAAAACAATAACACAAAGATACAACAACCa                                                                                        <  1:3119574/150‑1 (MQ=255)
                                                               caaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATgag                                                                                   <  2:2086222/150‑1 (MQ=255)
                                                                aaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATgaga                                                                                  <  2:811360/150‑1 (MQ=255)
                                                                 aGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAt                                                                                 <  2:1208466/150‑1 (MQ=255)
                                                                 aGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAt                                                                                 >  1:4454944/1‑150 (MQ=255)
                                                                   cAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTACTACCAAAAACAATAACACAAAGATACAACAACCAATGAGAttt                                                                               >  2:241002/1‑150 (MQ=255)
                                                                   cAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAttt                                                                               <  2:1076943/150‑1 (MQ=255)
                                                                   cAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAttt                                                                               <  2:4134209/150‑1 (MQ=255)
                                                                    aGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGTGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTa                                                                              >  1:2659746/1‑150 (MQ=255)
                                                                    aGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATNACACAAAGATACAACAACCAATGAGATTTa                                                                              <  1:4281606/150‑1 (MQ=255)
                                                                      gCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTaaa                                                                            >  2:623869/1‑150 (MQ=255)
                                                                      gCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTaaa                                                                            <  2:1557455/150‑1 (MQ=255)
                                                                             ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                     >  1:4467418/1‑150 (MQ=255)
                                                                             ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                     <  2:4033187/150‑1 (MQ=255)
                                                                               aaGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGc                                                                   <  2:2578180/150‑1 (MQ=255)
                                                                               aaGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGc                                                                   <  1:2427294/150‑1 (MQ=255)
                                                                                aGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTCGCa                                                                  >  1:790363/1‑150 (MQ=255)
                                                                                aGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCa                                                                  >  1:4600493/1‑150 (MQ=255)
                                                                                aGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCa                                                                  <  1:3469645/150‑1 (MQ=255)
                                                                                aGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCa                                                                  <  2:1780256/150‑1 (MQ=255)
                                                                                   gatgatATACGTTTTAAAACCAGGATTGTCAGAACGATTAACAGCAATCTCTGAACCATATGCTATCCGGAAGAAGCTACTAAGTTCGTAATACCAAAAACAATAAAACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAt                                                               <  1:1966452/150‑1 (MQ=255)
                                                                                    atgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAtt                                                              <  2:1238949/150‑1 (MQ=255)
                                                                                    atgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAtt                                                              <  2:1626325/150‑1 (MQ=255)
                                                                                     tgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAttt                                                             >  2:445138/1‑150 (MQ=255)
                                                                                      gatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttg                                                            >  1:2119853/1‑150 (MQ=255)
                                                                                        tataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgtt                                                          <  1:3170848/150‑1 (MQ=255)
                                                                                         ataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgttg                                                         >  2:4471575/1‑150 (MQ=255)
                                                                                            cGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAAt                                                      >  1:312239/1‑150 (MQ=255)
                                                                                             gTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATa                                                     <  2:3285887/150‑1 (MQ=255)
                                                                                              ttttACAACCAGGATGTTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATAc                                                    <  2:1204896/150‑1 (MQ=255)
                                                                                                  aCAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTc                                                <  2:322890/150‑1 (MQ=255)
                                                                                                    aaCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAt                                              <  1:326966/150‑1 (MQ=255)
                                                                                                    aaCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAt                                              >  1:3285737/1‑150 (MQ=255)
                                                                                                    aaCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAt                                              <  1:4638845/150‑1 (MQ=255)
                                                                                                    aaCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAt                                              <  2:2972432/150‑1 (MQ=255)
                                                                                                     aCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATc                                             <  1:4435652/150‑1 (MQ=255)
                                                                                                       cAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCa                                           <  2:494951/150‑1 (MQ=255)
                                                                                                          gATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaa                                        >  2:108688/1‑150 (MQ=255)
                                                                                                           aTGGTCAGAACGAAGAACAGCACGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAGCCAGaaa                                       >  2:28745/1‑150 (MQ=255)
                                                                                                           aTGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaaa                                       <  1:1335118/150‑1 (MQ=255)
                                                                                                            tGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAAt                                      <  2:1844936/150‑1 (MQ=255)
                                                                                                             ggTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATc                                     <  2:278042/150‑1 (MQ=255)
                                                                                                               tCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCa                                   >  1:729847/1‑150 (MQ=255)
                                                                                                                 aGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAg                                 >  2:3132192/1‑150 (MQ=255)
                                                                                                                 aGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAg                                 >  2:3400937/1‑150 (MQ=255)
                                                                                                                  gAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGt                                >  1:172011/1‑150 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                              <  1:2895785/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                              <  1:693116/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                              <  2:2518608/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                              >  1:599033/1‑150 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACAAAGTTCGTAATACCAAAAACAATAACACAAACATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATAATGTCATCCAGAAATCCAAGATc                              >  1:2161541/1‑150 (MQ=255)
                                                                                                                     cGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTca                             >  1:1679335/1‑150 (MQ=255)
                                                                                                                         gaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                         <  1:4214546/150‑1 (MQ=255)
                                                                                                                         gaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                         >  1:13825/1‑150 (MQ=255)
                                                                                                                            cAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCaa                      <  1:53498/150‑1 (MQ=255)
                                                                                                                               cAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAatat                   <  1:1130614/150‑1 (MQ=255)
                                                                                                                               cAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAatat                   <  1:185817/150‑1 (MQ=255)
                                                                                                                                aaGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAtata                  >  1:2623063/1‑150 (MQ=255)
                                                                                                                                    gcTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACa              >  1:69629/1‑150 (MQ=255)
                                                                                                                                     cTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACaa             >  2:417091/1‑150 (MQ=255)
                                                                                                                                      tGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAt            >  2:3692412/1‑150 (MQ=255)
                                                                                                                                          ccATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc        <  1:2831698/150‑1 (MQ=255)
                                                                                                                                              atGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaaca    <  2:444796/150‑1 (MQ=255)
                                                                                                                                              atGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaaca    <  2:2270247/150‑1 (MQ=255)
                                                                                                                                               tGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaacaa   <  1:3780727/150‑1 (MQ=255)
                                                                                                                                               tGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaacaa   <  2:3186037/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAAT  >  CP009273/3314723‑3315016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314724‑3315019
                                                                                                                                                  |                                                                                                                                                     
ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCC                                                                                                                                                    >  SRR19973072.1849784/1‑150 (MQ=60)
ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCC                                                                                                                                                    >  SRR19973072.1848934/1‑150 (MQ=60)
ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCC                                                                                                                                                    >  SRR19973072.2157911/1‑150 (MQ=60)
 CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCG                                                                                                                                                   <  SRR19973072.402501/150‑1 (MQ=60)
    CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                >  SRR19973072.2890909/1‑150 (MQ=60)
    CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                <  SRR19973072.3984511/150‑1 (MQ=60)
    CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                <  SRR19973072.3984161/150‑1 (MQ=60)
     ATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAG                                                                                                                                               <  SRR19973072.2097192/150‑1 (MQ=60)
     ATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAG                                                                                                                                               <  SRR19973072.2097524/150‑1 (MQ=60)
       GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                             >  SRR19973072.1313123/1‑150 (MQ=60)
       GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                             <  SRR19973072.3735192/150‑1 (MQ=60)
                CTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAG                                                                                                                                    <  SRR19973072.3788583/150‑1 (MQ=60)
                 TAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGT                                                                                                                                   >  SRR19973072.4095216/1‑150 (MQ=60)
                 TAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGT                                                                                                                                   >  SRR19973072.1768309/1‑150 (MQ=60)
                  AATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTT                                                                                                                                  <  SRR19973072.3000986/150‑1 (MQ=60)
                     ACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGT                                                                                                                               <  SRR19973072.4059315/150‑1 (MQ=60)
                     ACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGT                                                                                                                               >  SRR19973072.4088074/1‑150 (MQ=60)
                     ACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGT                                                                                                                               >  SRR19973072.4343949/1‑150 (MQ=60)
                        CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            <  SRR19973072.3895261/150‑1 (MQ=60)
                         ATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATA                                                                                                                           <  SRR19973072.3053768/150‑1 (MQ=60)
                          TGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATAC                                                                                                                          >  SRR19973072.20601/1‑150 (MQ=60)
                           GTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACC                                                                                                                         >  SRR19973072.2700442/1‑150 (MQ=60)
                           GTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACC                                                                                                                         >  SRR19973072.2362129/1‑150 (MQ=60)
                              TGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAA                                                                                                                      <  SRR19973072.960350/150‑1 (MQ=60)
                                CCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAA                                                                                                                    <  SRR19973072.1020875/150‑1 (MQ=60)
                                       CAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAC                                                                                                             >  SRR19973072.3192756/1‑150 (MQ=60)
                                       CAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAC                                                                                                             <  SRR19973072.3834320/150‑1 (MQ=60)
                                       CAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAC                                                                                                             >  SRR19973072.3192750/1‑150 (MQ=60)
                                       CAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAC                                                                                                             >  SRR19973072.1036251/1‑150 (MQ=60)
                                       CAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAC                                                                                                             >  SRR19973072.1036444/1‑150 (MQ=60)
                                       CAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAC                                                                                                             >  SRR19973072.1037307/1‑150 (MQ=60)
                                         GTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACAC                                                                                                           <  SRR19973072.4226515/150‑1 (MQ=60)
                                            ACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAA                                                                                                        <  SRR19973072.2974887/150‑1 (MQ=60)
                                              ACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATGTACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGA                                                                                                      >  SRR19973072.3781080/1‑150 (MQ=60)
                                              ACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGA                                                                                                      <  SRR19973072.132109/150‑1 (MQ=60)
                                                ATATGGTGCGTCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCTATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATA                                                                                                    <  SRR19973072.1378447/150‑1 (MQ=60)
                                                AAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATA                                                                                                    <  SRR19973072.1902601/150‑1 (MQ=60)
                                                 AATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATAC                                                                                                   <  SRR19973072.4185245/150‑1 (MQ=60)
                                                    GGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTTATACCAAAAACAATAACACAAAGATACAAC                                                                                                <  SRR19973072.2387007/150‑1 (MQ=60)
                                                    GGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAAC                                                                                                <  SRR19973072.69571/150‑1 (MQ=60)
                                                     GTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACA                                                                                               >  SRR19973072.3433483/1‑150 (MQ=60)
                                                     GTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACA                                                                                               >  SRR19973072.3433351/1‑150 (MQ=60)
                                                       GCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAAC                                                                                             <  SRR19973072.1916842/150‑1 (MQ=60)
                                                         GGCAACAAGCAGGCTAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCA                                                                                           <  SRR19973072.2059378/150‑1 (MQ=60)
                                                         GGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCA                                                                                           <  SRR19973072.3126188/150‑1 (MQ=60)
                                                         GGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTCCGTAATACCAAAAACAATAACACAAAGATACAACAACCA                                                                                           <  SRR19973072.3126154/150‑1 (MQ=60)
                                                            AACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATG                                                                                        <  SRR19973072.548349/150‑1 (MQ=60)
                                                              CAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAG                                                                                      <  SRR19973072.4198533/150‑1 (MQ=60)
                                                                AGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAT                                                                                    >  SRR19973072.4464340/1‑150 (MQ=60)
                                                                 GCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATT                                                                                   >  SRR19973072.3320699/1‑150 (MQ=60)
                                                                   AGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGTGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTA                                                                                 >  SRR19973072.2665363/1‑150 (MQ=60)
                                                                   AGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGTGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTA                                                                                 >  SRR19973072.2665441/1‑150 (MQ=60)
                                                                   AGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATNACACAAAGATACAACAACCAATGAGATTTA                                                                                 <  SRR19973072.4290515/150‑1 (MQ=60)
                                                                   AGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTA                                                                                 <  SRR19973072.3007282/150‑1 (MQ=60)
                                                                   AGGCGAAGCGGAAGGGATGATATAAGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGATGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATAACAAAAACAATAACACACAGATACAACAACCAATGAGATTTA                                                                                 <  SRR19973072.785697/150‑1 (MQ=60)
                                                                       GAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCTCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAG                                                                             <  SRR19973072.3693929/150‑1 (MQ=60)
                                                                       GAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAG                                                                             <  SRR19973072.2900823/150‑1 (MQ=60)
                                                                          GCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATTTGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTC                                                                          <  SRR19973072.709458/150‑1 (MQ=60)
                                                                           CGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCT                                                                         >  SRR19973072.793695/1‑150 (MQ=60)
                                                                            GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                        >  SRR19973072.4476813/1‑150 (MQ=60)
                                                                            GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                        >  SRR19973072.4476843/1‑150 (MQ=60)
                                                                              AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      <  SRR19973072.2432556/150‑1 (MQ=60)
                                                                               AGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTCGCA                                                                     >  SRR19973072.792050/1‑150 (MQ=60)
                                                                               AGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCA                                                                     >  SRR19973072.4610147/1‑150 (MQ=60)
                                                                               AGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCA                                                                     <  SRR19973072.3476900/150‑1 (MQ=60)
                                                                                  GATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACCACAACCTATGAGATTTAAAAGTTCTAGCAAAT                                                                  <  SRR19973072.1970770/150‑1 (MQ=60)
                                                                                  GATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAT                                                                  >  SRR19973072.1641738/1‑150 (MQ=60)
                                                                                  GATGATATACGTTTTAAAACCAGGATTGTCAGAACGATTAACAGCAATCTCTGAACCATATGCTATCCGGAAGAAGCTACTAAGTTCGTAATACCAAAAACAATAAAACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAT                                                                  <  SRR19973072.1970660/150‑1 (MQ=60)
                                                                                    TGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTT                                                                >  SRR19973072.1072786/1‑150 (MQ=60)
                                                                                     GATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTG                                                               >  SRR19973072.2124474/1‑150 (MQ=60)
                                                                                       TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                             <  SRR19973072.1022573/150‑1 (MQ=60)
                                                                                       TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                             <  SRR19973072.3177534/150‑1 (MQ=60)
                                                                                       TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                             <  SRR19973072.3177659/150‑1 (MQ=60)
                                                                                         TACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGA                                                           <  SRR19973072.3264220/150‑1 (MQ=60)
                                                                                           CGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAAT                                                         >  SRR19973072.312956/1‑150 (MQ=60)
                                                                                           CGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAAT                                                         >  SRR19973072.3758178/1‑150 (MQ=60)
                                                                                                  CAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCA                                                  >  SRR19973072.3286683/1‑150 (MQ=60)
                                                                                                   AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAT                                                 >  SRR19973072.3292712/1‑150 (MQ=60)
                                                                                                   AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAT                                                 <  SRR19973072.327702/150‑1 (MQ=60)
                                                                                                   AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAT                                                 <  SRR19973072.4648547/150‑1 (MQ=60)
                                                                                                   AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATATAAAAGTTCTAGCAAATTTGTTGAATACTGTCAT                                                 >  SRR19973072.4584664/1‑150 (MQ=60)
                                                                                                    ACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATC                                                <  SRR19973072.4445008/150‑1 (MQ=60)
                                                                                                       AGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAG                                             <  SRR19973072.4225878/150‑1 (MQ=60)
                                                                                                          ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973072.4448445/1‑150 (MQ=60)
                                                                                                          ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          <  SRR19973072.1338037/150‑1 (MQ=60)
                                                                                                           TGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAAT                                         <  SRR19973072.3728499/150‑1 (MQ=60)
                                                                                                              TCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCA                                      >  SRR19973072.731420/1‑150 (MQ=60)
                                                                                                                 GAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGT                                   >  SRR19973072.172167/1‑150 (MQ=60)
                                                                                                                 GAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGT                                   <  SRR19973072.1920292/150‑1 (MQ=60)
                                                                                                                 GAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGT                                   >  SRR19973072.172448/1‑150 (MQ=60)
                                                                                                                   ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                 >  SRR19973072.600416/1‑150 (MQ=60)
                                                                                                                   ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                 <  SRR19973072.694639/150‑1 (MQ=60)
                                                                                                                   ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                 <  SRR19973072.2901988/150‑1 (MQ=60)
                                                                                                                   ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                 <  SRR19973072.2835174/150‑1 (MQ=60)
                                                                                                                   ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACAAAGTTCGTAATACCAAAAACAATAACACAAACATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATAATGTCATCCAGAAATCCAAGATC                                 >  SRR19973072.2166251/1‑150 (MQ=60)
                                                                                                                    CGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCA                                >  SRR19973072.1683008/1‑150 (MQ=60)
                                                                                                                       AGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCAAGAAATCCAAGTTCACAA                             >  SRR19973072.3152551/1‑150 (MQ=60)
                                                                                                                        GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                            >  SRR19973072.13872/1‑150 (MQ=60)
                                                                                                                        GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                            >  SRR19973072.4625028/1‑150 (MQ=60)
                                                                                                                        GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                            <  SRR19973072.4223346/150‑1 (MQ=60)
                                                                                                                          ACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCA                          <  SRR19973072.689420/150‑1 (MQ=60)
                                                                                                                          ACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCA                          <  SRR19973072.689448/150‑1 (MQ=60)
                                                                                                                           CAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAA                         <  SRR19973072.53604/150‑1 (MQ=60)
                                                                                                                           CAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAA                         <  SRR19973072.53636/150‑1 (MQ=60)
                                                                                                                           CAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAA                         <  SRR19973072.2091185/150‑1 (MQ=60)
                                                                                                                            AGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAT                        >  SRR19973072.452175/1‑150 (MQ=60)
                                                                                                                              CAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATAT                      <  SRR19973072.1132982/150‑1 (MQ=60)
                                                                                                                              CAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATAT                      <  SRR19973072.186284/150‑1 (MQ=60)
                                                                                                                               AAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATA                     >  SRR19973072.2628706/1‑150 (MQ=60)
                                                                                                                               AAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATA                     >  SRR19973072.2628584/1‑150 (MQ=60)
                                                                                                                                   GCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACA                 >  SRR19973072.2690911/1‑150 (MQ=60)
                                                                                                                                   GCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACA                 >  SRR19973072.69811/1‑150 (MQ=60)
                                                                                                                                         CCATATGCGATCCGGAAGAATCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTATCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973072.2837721/150‑1 (MQ=60)
                                                                                                                                         CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973072.2837763/150‑1 (MQ=60)
                                                                                                                                         CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973072.2837762/150‑1 (MQ=60)
                                                                                                                                          CATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCA          <  SRR19973072.1835965/150‑1 (MQ=60)
                                                                                                                                              TGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAA      <  SRR19973072.3788657/150‑1 (MQ=60)
                                                                                                                                                 GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT   >  SRR19973072.830497/1‑150 (MQ=60)
                                                                                                                                                 GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT   >  SRR19973072.830601/1‑150 (MQ=60)
                                                                                                                                                 GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT   >  SRR19973072.955614/1‑150 (MQ=60)
                                                                                                                                                  ATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  <  SRR19973072.4204116/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314724‑3315019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑