Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,314,870 C→A 62.7% A35S (GCG→TCG)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,314,8700CA62.7% 120.9 / 189.2 150A35S (GCG→TCG) yhbXputative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (17/39);  new base A (32/62);  total (49/101)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 152 total aligned reads displayed.

ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314724‑3315019
                                                                                                                                                  |                                                                                                                                                     
acacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATcc                                                                                                                                                    <  2:3027218/150‑1 (MQ=255)
 cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCg                                                                                                                                                   <  1:4193269/150‑1 (MQ=255)
 cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCg                                                                                                                                                   >  1:3376780/1‑150 (MQ=255)
       gAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaagac                                                                                                                                             >  2:1865624/1‑149 (MQ=255)
          tAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCg                                                                                                                                          <  1:547382/150‑1 (MQ=255)
           agagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGa                                                                                                                                         <  1:312579/150‑1 (MQ=255)
           agagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGa                                                                                                                                         <  1:2146044/150‑1 (MQ=255)
           agagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGa                                                                                                                                         <  1:2146676/150‑1 (MQ=255)
             agGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACt                                                                                                                                       <  2:1982838/150‑1 (MQ=255)
              ggCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTa                                                                                                                                      >  1:4649582/1‑150 (MQ=255)
               gCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTaa                                                                                                                                     >  2:1716186/1‑150 (MQ=255)
                cTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAg                                                                                                                                    >  1:3404547/1‑150 (MQ=255)
                 tAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGt                                                                                                                                   >  2:3304995/1‑150 (MQ=255)
                  aaTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGtt                                                                                                                                  >  1:3898699/1‑150 (MQ=255)
                   aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATTATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTc                                                                                                                                 <  1:2344943/150‑1 (MQ=255)
                   aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTc                                                                                                                                 <  2:3315177/150‑1 (MQ=255)
                    tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCg                                                                                                                                >  1:85253/1‑150 (MQ=255)
                     aCTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGt                                                                                                                               >  1:3291072/1‑150 (MQ=255)
                       tCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTaa                                                                                                                             <  2:3498340/150‑1 (MQ=255)
                        cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                            <  2:3739679/150‑1 (MQ=255)
                           gTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATAcc                                                                                                                         <  1:3497609/150‑1 (MQ=255)
                               gCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCaaaa                                                                                                                     <  2:2328508/150‑1 (MQ=255)
                               gCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCaaaa                                                                                                                     <  1:4757767/150‑1 (MQ=255)
                                  gCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACa                                                                                                                  <  2:2095716/150‑1 (MQ=255)
                                     gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                               >  2:2266929/1‑150 (MQ=255)
                                     gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                               <  2:429730/150‑1 (MQ=255)
                                     gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                               <  1:2636595/150‑1 (MQ=255)
                                     gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                               >  2:2710479/1‑150 (MQ=255)
                                          tAACACAAATGGTGCGGCAACTAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAcaca                                                                                                          <  2:1593029/150‑1 (MQ=255)
                                          tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAcaca                                                                                                          >  2:321106/1‑150 (MQ=255)
                                           aaCACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACaa                                                                                                         >  1:818047/1‑150 (MQ=255)
                                           aaCACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACaa                                                                                                         <  1:2882062/150‑1 (MQ=255)
                                            acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAATGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACaaa                                                                                                        <  1:608344/150‑1 (MQ=255)
                                              acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGa                                                                                                      <  2:830997/150‑1 (MQ=255)
                                              acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGa                                                                                                      <  2:834180/150‑1 (MQ=255)
                                                  aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATaca                                                                                                  <  1:2826170/150‑1 (MQ=255)
                                                  aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATaca                                                                                                  >  2:3856758/1‑150 (MQ=255)
                                                   tGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACTTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATacaa                                                                                                 <  1:1236752/150‑1 (MQ=255)
                                                   tGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATacaa                                                                                                 <  2:3063171/150‑1 (MQ=255)
                                                         ggCAACAAGCTGGCGATGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCa                                                                                           <  2:3629384/150‑1 (MQ=255)
                                                         ggCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCa                                                                                           <  1:2284323/150‑1 (MQ=255)
                                                          gCAACAAGCAGGCGTAGCGGAAGGGATGATATACGTTTTAAAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCaa                                                                                          <  2:1519412/150‑1 (MQ=255)
                                                           caacaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAAt                                                                                         >  1:657091/1‑150 (MQ=255)
                                                            aacaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATg                                                                                        >  1:3961163/1‑150 (MQ=255)
                                                                 gCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAtt                                                                                   <  2:4075294/150‑1 (MQ=255)
                                                                 gCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAtt                                                                                   <  2:879581/150‑1 (MQ=255)
                                                                  cAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAttt                                                                                  <  2:1833381/150‑1 (MQ=255)
                                                                     gCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTaaa                                                                               <  2:1744762/150‑1 (MQ=255)
                                                                     gCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAAATTTaaa                                                                               <  2:250951/150‑1 (MQ=255)
                                                                       taaGAGGAAGGGATGATATACTTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGTAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAg                                                                             <  1:948228/149‑1 (MQ=255)
                                                                            tgAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                        <  2:1430298/149‑1 (MQ=255)
                                                                            ggAAGTTATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCTATCCGTAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                        <  2:2517643/150‑1 (MQ=255)
                                                                            ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                        <  1:1781054/150‑1 (MQ=255)
                                                                             gAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAg                                                                       <  1:1895867/150‑1 (MQ=255)
                                                                              aaGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGc                                                                      <  1:4480547/150‑1 (MQ=255)
                                                                              aaGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGc                                                                      <  1:4480560/150‑1 (MQ=255)
                                                                                  gatgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAt                                                                  <  1:3921753/150‑1 (MQ=255)
                                                                                  gatgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAt                                                                  <  1:4014786/150‑1 (MQ=255)
                                                                                     gatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttg                                                               >  2:2065631/1‑150 (MQ=255)
                                                                                      atatACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgt                                                              >  1:4168059/1‑150 (MQ=255)
                                                                                       tataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgtt                                                             >  1:1565094/1‑150 (MQ=255)
                                                                                       tataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgtt                                                             >  1:3319007/1‑150 (MQ=255)
                                                                                        ataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgttg                                                            >  2:212792/1‑150 (MQ=255)
                                                                                        ataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgtta                                                            <  2:227678/150‑2 (MQ=255)
                                                                                         taCTTTTTACAACCAGTATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGa                                                           <  2:4340992/150‑1 (MQ=255)
                                                                                         taCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGa                                                           <  2:4023351/150‑1 (MQ=255)
                                                                                         taCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGa                                                           <  1:3299956/150‑1 (MQ=255)
                                                                                          aCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGaa                                                          <  2:85253/150‑1 (MQ=255)
                                                                                           cGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACC‑AAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATa                                                        >  1:1817852/1‑150 (MQ=255)
                                                                                            gTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCTCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATa                                                        <  2:403032/150‑1 (MQ=255)
                                                                                              tttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACt                                                      >  1:3950852/1‑150 (MQ=255)
                                                                                               ttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTg                                                     >  1:2130766/1‑150 (MQ=255)
                                                                                               ttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTg                                                     >  1:2130665/1‑150 (MQ=255)
                                                                                               ttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTg                                                     >  1:4743689/1‑150 (MQ=255)
                                                                                                tACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGt                                                    >  1:2504688/1‑150 (MQ=255)
                                                                                                 aCAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTc                                                   <  2:1937170/150‑1 (MQ=255)
                                                                                                 aCAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTc                                                   <  1:537584/150‑1 (MQ=255)
                                                                                                       aGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAg                                             <  1:120222/150‑1 (MQ=255)
                                                                                                       aGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAg                                             <  1:705403/150‑1 (MQ=255)
                                                                                                       aGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAg                                             <  2:4743689/150‑1 (MQ=255)
                                                                                                        ggATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGa                                            <  1:1307672/150‑1 (MQ=255)
                                                                                                           tGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAAt                                         >  2:538213/1‑150 (MQ=255)
                                                                                                            ggTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATc                                        <  2:282275/150‑1 (MQ=255)
                                                                                                              tCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCa                                      <  2:245996/150‑1 (MQ=255)
                                                                                                              tCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCa                                      <  2:4573774/150‑1 (MQ=255)
                                                                                                                    cGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTca                                <  1:1033563/150‑1 (MQ=255)
                                                                                                                     gaagaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTcac                               <  2:340932/150‑1 (MQ=255)
                                                                                                                     gaagaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTcac                               <  1:155132/150‑1 (MQ=255)
                                                                                                                      aagaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTcaca                              <  1:4018085/150‑1 (MQ=255)
                                                                                                                        gaaCAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                            >  2:1042398/1‑150 (MQ=255)
                                                                                                                        gaaCAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                            >  2:1042401/1‑150 (MQ=255)
                                                                                                                        gaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                            >  1:4077428/1‑150 (MQ=255)
                                                                                                                        gaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                            >  1:4077333/1‑150 (MQ=255)
                                                                                                                            aGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAat                        <  1:3287246/150‑1 (MQ=255)
                                                                                                                               aaGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAtata                     <  2:4398658/150‑1 (MQ=255)
                                                                                                                                    cTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACaa                <  2:829299/150‑1 (MQ=255)
                                                                                                                                         ccATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc           <  1:3770805/150‑1 (MQ=255)
                                                                                                                                         ccATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc           <  1:1486886/150‑1 (MQ=255)
                                                                                                                                              tGCGCTACGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaacaa      <  2:4537701/150‑1 (MQ=255)
                                                                                                                                                  cTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTATCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGtt  <  2:4392853/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314724‑3315019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314725‑3315019
                                                                                                                                                 |                                                                                                                                                     
CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCG                                                                                                                                                   >  SRR19973082.3397662/1‑150 (MQ=60)
CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCG                                                                                                                                                   <  SRR19973082.4218884/150‑1 (MQ=60)
CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCG                                                                                                                                                   >  SRR19973082.2036661/1‑150 (MQ=60)
      GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGCGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                             >  SRR19973082.3488820/1‑150 (MQ=60)
         TAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCG                                                                                                                                          <  SRR19973082.551313/150‑1 (MQ=60)
          AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGA                                                                                                                                         <  SRR19973082.314770/150‑1 (MQ=60)
          AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGA                                                                                                                                         <  SRR19973082.2160321/150‑1 (MQ=60)
          AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGA                                                                                                                                         <  SRR19973082.2159688/150‑1 (MQ=60)
          AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGA                                                                                                                                         <  SRR19973082.2159560/150‑1 (MQ=60)
           GAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGAC                                                                                                                                        <  SRR19973082.1472487/150‑1 (MQ=60)
             GGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTA                                                                                                                                      >  SRR19973082.4677712/1‑150 (MQ=60)
               CTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAG                                                                                                                                    >  SRR19973082.3425573/1‑150 (MQ=60)
                 AATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTT                                                                                                                                  >  SRR19973082.3922534/1‑150 (MQ=60)
                  ATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATTATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTC                                                                                                                                 <  SRR19973082.2359416/150‑1 (MQ=60)
                   TACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCG                                                                                                                                >  SRR19973082.86141/1‑150 (MQ=60)
                    ACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGT                                                                                                                               >  SRR19973082.3311393/1‑150 (MQ=60)
                       CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            >  SRR19973082.3081932/1‑150 (MQ=60)
                          GTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACC                                                                                                                         <  SRR19973082.3519008/150‑1 (MQ=60)
                              GCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAA                                                                                                                     <  SRR19973082.4786270/150‑1 (MQ=60)
                                    GTCATTAACAAAAATGGTGCGGCAACAAGCAGGAGAAGGGGAAGGTATGATATACGTTTTACAAACAGGATGGTCAGAACGAAGAACAGCAAGCTCTTAACCATATGCGATCCGTAAGAAGCGACTAAGTTCGTAATACCAAAAACAATA                                                                                                               <  SRR19973082.4551625/150‑1 (MQ=60)
                                    GTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATA                                                                                                               <  SRR19973082.2652899/150‑1 (MQ=60)
                                       AGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACA                                                                                                            >  SRR19973082.2445015/1‑150 (MQ=60)
                                          AACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAA                                                                                                         >  SRR19973082.823392/1‑150 (MQ=60)
                                          AACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAA                                                                                                         <  SRR19973082.2899848/150‑1 (MQ=60)
                                           ACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAATGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAA                                                                                                        <  SRR19973082.612661/150‑1 (MQ=60)
                                                 ATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACA                                                                                                  <  SRR19973082.2843484/150‑1 (MQ=60)
                                                  TGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACTTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAA                                                                                                 <  SRR19973082.1245222/150‑1 (MQ=60)
                                                        GGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCA                                                                                           <  SRR19973082.2298587/150‑1 (MQ=60)
                                                          CAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAAT                                                                                         >  SRR19973082.661630/1‑150 (MQ=60)
                                                           AACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATG                                                                                        >  SRR19973082.3985209/1‑150 (MQ=60)
                                                             CAAGCAGGCGAAGCGGATGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAG                                                                                      <  SRR19973082.825312/150‑1 (MQ=60)
                                                                      taagaGGAAGGGATGATATACTTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGTAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAG                                                                             <  SRR19973082.954815/145‑1 (MQ=60)
                                                                      GAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAG                                                                             <  SRR19973082.954860/150‑1 (MQ=60)
                                                                           GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                        <  SRR19973082.1792798/150‑1 (MQ=60)
                                                                            GAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAG                                                                       <  SRR19973082.1908029/150‑1 (MQ=60)
                                                                             AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      <  SRR19973082.4507782/150‑1 (MQ=60)
                                                                             AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      <  SRR19973082.4507769/150‑1 (MQ=60)
                                                                             AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      <  SRR19973082.1218434/150‑1 (MQ=60)
                                                                                 GATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAT                                                                  <  SRR19973082.4039344/150‑1 (MQ=60)
                                                                                 GATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAT                                                                  <  SRR19973082.3945668/150‑1 (MQ=60)
                                                                                 GATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAT                                                                  <  SRR19973082.4039044/150‑1 (MQ=60)
                                                                                  ATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATT                                                                 >  SRR19973082.4273049/1‑150 (MQ=60)
                                                                                     ATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGT                                                              >  SRR19973082.4193568/1‑150 (MQ=60)
                                                                                      TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                             >  SRR19973082.1575310/1‑150 (MQ=60)
                                                                                      TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                             >  SRR19973082.3339530/1‑150 (MQ=60)
                                                                                      TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                             >  SRR19973082.3339666/1‑150 (MQ=60)
                                                                                        TACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGA                                                           <  SRR19973082.3320343/150‑1 (MQ=60)
                                                                                          CGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACC‑AAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATA                                                        >  SRR19973082.1829751/1‑150 (MQ=60)
                                                                                             TTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACT                                                      >  SRR19973082.3974859/1‑150 (MQ=60)
                                                                                             TTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACT                                                      >  SRR19973082.3974857/1‑150 (MQ=60)
                                                                                              TTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTG                                                     <  SRR19973082.3325415/150‑1 (MQ=60)
                                                                                              TTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTG                                                     >  SRR19973082.2144213/1‑150 (MQ=60)
                                                                                              TTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTG                                                     >  SRR19973082.4772154/1‑150 (MQ=60)
                                                                                              TTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTG                                                     >  SRR19973082.2144314/1‑150 (MQ=60)
                                                                                               TACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGT                                                    >  SRR19973082.2520327/1‑150 (MQ=60)
                                                                                                ACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTC                                                   <  SRR19973082.541461/150‑1 (MQ=60)
                                                                                                ACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTC                                                   <  SRR19973082.541439/150‑1 (MQ=60)
                                                                                                      AGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAG                                             <  SRR19973082.121337/150‑1 (MQ=60)
                                                                                                      AGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAG                                             <  SRR19973082.710157/150‑1 (MQ=60)
                                                                                                       GGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGA                                            <  SRR19973082.1316713/150‑1 (MQ=60)
                                                                                                          TGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAAT                                         <  SRR19973082.2281133/150‑1 (MQ=60)
                                                                                                               AGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAG                                    <  SRR19973082.3311296/150‑1 (MQ=60)
                                                                                                                   CGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCA                                <  SRR19973082.1040705/150‑1 (MQ=60)
                                                                                                                    GAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCAC                               <  SRR19973082.4052926/150‑1 (MQ=60)
                                                                                                                    GAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCAC                               <  SRR19973082.156500/150‑1 (MQ=60)
                                                                                                                     AAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACA                              <  SRR19973082.4042681/150‑1 (MQ=60)
                                                                                                                      AGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAA                             >  SRR19973082.810093/1‑150 (MQ=60)
                                                                                                                       GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                            >  SRR19973082.4102528/1‑150 (MQ=60)
                                                                                                                       GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                            >  SRR19973082.4102414/1‑150 (MQ=60)
                                                                                                                       GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                            >  SRR19973082.4102318/1‑150 (MQ=60)
                                                                                                                          CAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAA                         >  SRR19973082.654652/1‑150 (MQ=60)
                                                                                                                           AGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAT                        <  SRR19973082.3307541/150‑1 (MQ=60)
                                                                                                                                 CGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAAC                  <  SRR19973082.4781208/150‑1 (MQ=60)
                                                                                                                                    TGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAT               <  SRR19973082.1799239/150‑1 (MQ=60)
                                                                                                                                        CCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973082.3794093/150‑1 (MQ=60)
                                                                                                                                        CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973082.1496845/150‑1 (MQ=60)
                                                                                                                                           TATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAAC        <  SRR19973082.2727024/150‑1 (MQ=60)
                                                                                                                                           TATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAAC        <  SRR19973082.3880434/150‑1 (MQ=60)
                                                                                                                                                 CTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  <  SRR19973082.157025/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314725‑3315019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑