Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,314,870 C→A 100% A35S (GCG→TCG)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,314,8700CA96.6% 669.3 / 14.4 207A35S (GCG→TCG) yhbXputative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base A (91/109);  total (97/110)
Fisher's exact test for biased strand distribution p-value = 5.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314723‑3315019
                                                                                                                                                   |                                                                                                                                                     
   acCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCgg                                                                                                                                                  >  1:269333/1‑150 (MQ=255)
     cATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaa                                                                                                                                                <  1:625861/150‑1 (MQ=255)
     cATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaa                                                                                                                                                >  1:3366687/1‑150 (MQ=255)
     cATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaa                                                                                                                                                >  2:2516039/1‑150 (MQ=255)
        gAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACACGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaagaa                                                                                                                                             <  1:2574050/150‑1 (MQ=255)
        gAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaagaa                                                                                                                                             >  1:731119/1‑150 (MQ=255)
        gAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaagaa                                                                                                                                             <  1:1853067/150‑1 (MQ=255)
          aTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCTGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGc                                                                                                                                           <  2:2398486/150‑1 (MQ=255)
             gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGAc                                                                                                                                        >  2:3989621/1‑150 (MQ=255)
               ggCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTa                                                                                                                                      <  2:2940164/150‑1 (MQ=255)
               ggCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTa                                                                                                                                      <  2:2792484/150‑1 (MQ=255)
                 cTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAg                                                                                                                                    <  2:3370753/150‑1 (MQ=255)
                  tAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGt                                                                                                                                   >  1:3223709/1‑150 (MQ=255)
                   aaTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGtt                                                                                                                                  <  2:1127150/150‑1 (MQ=255)
                    aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTc                                                                                                                                 >  2:197352/1‑150 (MQ=255)
                      aCTCATTTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGt                                                                                                                               >  1:1988538/1‑150 (MQ=255)
                        tCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTaa                                                                                                                             <  2:2158594/150‑1 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                            >  1:15614/1‑150 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                            >  1:15808/1‑150 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                            <  2:137660/150‑1 (MQ=255)
                          aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATa                                                                                                                           >  1:1029180/1‑150 (MQ=255)
                          aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATa                                                                                                                           <  1:1680839/150‑1 (MQ=255)
                                      gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                               <  2:1034272/150‑1 (MQ=255)
                                      gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                               <  1:3141117/150‑1 (MQ=255)
                                       tcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATaa                                                                                                              <  2:669546/150‑1 (MQ=255)
                                          gtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAacac                                                                                                           <  2:1270537/150‑1 (MQ=255)
                                            aaCACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACaa                                                                                                         >  1:1279815/1‑150 (MQ=255)
                                            aaCACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACaa                                                                                                         >  1:3449699/1‑150 (MQ=255)
                                            aaCACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACaa                                                                                                         >  1:2056261/1‑150 (MQ=255)
                                              cacaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGTAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAg                                                                                                       <  2:3955779/150‑1 (MQ=255)
                                              cacaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAg                                                                                                       <  2:886084/150‑1 (MQ=255)
                                               acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGa                                                                                                      >  1:2649282/1‑150 (MQ=255)
                                                caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGAt                                                                                                     <  2:3310658/150‑1 (MQ=255)
                                                 aaaTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATa                                                                                                    >  2:2642261/1‑150 (MQ=255)
                                                     ggTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATacaac                                                                                                <  2:4086310/150‑1 (MQ=255)
                                                       tGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATAcaacaa                                                                                              >  2:1231254/1‑150 (MQ=255)
                                                          ggCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCa                                                                                           <  1:914566/150‑1 (MQ=255)
                                                             aacaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATg                                                                                        >  1:3170806/1‑150 (MQ=255)
                                                             aacaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATg                                                                                        <  2:3014142/150‑1 (MQ=255)
                                                               caaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATgag                                                                                      <  1:2392483/150‑1 (MQ=255)
                                                                  gCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAtt                                                                                   >  1:2176665/1‑150 (MQ=255)
                                                                    aGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTa                                                                                 >  1:2501707/1‑150 (MQ=255)
                                                                     ggCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTaa                                                                                <  2:274078/150‑1 (MQ=255)
                                                                       cGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTaaaa                                                                              >  1:1152593/1‑150 (MQ=255)
                                                                             ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                        <  1:3279185/150‑1 (MQ=255)
                                                                             ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                        <  2:3773834/150‑1 (MQ=255)
                                                                                aGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCa                                                                     <  2:3080906/150‑1 (MQ=255)
                                                                                 gggATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCaa                                                                    >  1:579260/1‑150 (MQ=255)
                                                                                  ggATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCaaa                                                                   >  2:1419071/1‑150 (MQ=255)
                                                                                  ggATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGATCTAGCaaa                                                                   >  2:1419585/1‑150 (MQ=255)
                                                                                    atgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTAGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAtt                                                                 <  2:3169923/150‑1 (MQ=255)
                                                                                     tgatATACGTTTTACAACCAGGATTGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAttt                                                                >  1:720275/1‑150 (MQ=255)
                                                                                       atatACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgt                                                              >  2:35601/1‑150 (MQ=255)
                                                                                        tataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgtt                                                             <  2:1284307/150‑1 (MQ=255)
                                                                                         ataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgttg                                                            <  1:2955870/150‑1 (MQ=255)
                                                                                         ataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgttg                                                            >  1:2610886/1‑150 (MQ=255)
                                                                                         ataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgttg                                                            >  1:1329141/1‑150 (MQ=255)
                                                                                           aCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGaa                                                          <  2:359201/150‑1 (MQ=255)
                                                                                            cGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAAt                                                         <  1:548395/150‑1 (MQ=255)
                                                                                             gTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATa                                                        <  1:2940787/150‑1 (MQ=255)
                                                                                               tttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGactact                                                      >  2:2825876/1‑150 (MQ=255)
                                                                                                ttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTg                                                     >  1:3336181/1‑150 (MQ=255)
                                                                                                  aCAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTc                                                   <  2:3651592/150‑1 (MQ=255)
                                                                                                   cAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCa                                                  <  2:55641/150‑1 (MQ=255)
                                                                                                   cAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCa                                                  >  2:467885/1‑150 (MQ=255)
                                                                                                     aCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATc                                                >  1:2285596/1‑150 (MQ=255)
                                                                                                     aCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATc                                                >  1:204125/1‑150 (MQ=255)
                                                                                                       cAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCa                                              >  1:1206971/1‑150 (MQ=255)
                                                                                                       cAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCa                                              >  2:1791963/1‑150 (MQ=255)
                                                                                                        aGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAg                                             >  2:2143991/1‑150 (MQ=255)
                                                                                                          gATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaa                                           <  2:3907397/150‑1 (MQ=255)
                                                                                                          gATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaa                                           <  2:267944/150‑1 (MQ=255)
                                                                                                           aTGGTCAGAACGAAGAACAGCAAGCGCTGANCCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaaa                                          >  1:3852673/1‑150 (MQ=255)
                                                                                                           aTGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaaa                                          >  1:633554/1‑150 (MQ=255)
                                                                                                           aTGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGaaa                                          >  1:633618/1‑150 (MQ=255)
                                                                                                             ggTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATc                                        <  1:1448027/150‑1 (MQ=255)
                                                                                                             ggTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATc                                        >  1:2121249/1‑150 (MQ=255)
                                                                                                             ggTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATc                                        >  1:2209718/1‑150 (MQ=255)
                                                                                                               tCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCa                                      >  2:3133334/1‑150 (MQ=255)
                                                                                                                cAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCaa                                     >  1:3914675/1‑150 (MQ=255)
                                                                                                                cAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCaa                                     >  1:3705544/1‑150 (MQ=255)
                                                                                                                cAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCaa                                     >  2:1546990/1‑150 (MQ=255)
                                                                                                                   aaCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGtt                                  <  1:3299699/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCTTATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                                 <  2:756345/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                                 <  1:3609852/150‑1 (MQ=255)
                                                                                                                          aaCAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAcc                           <  2:2199365/150‑1 (MQ=255)
                                                                                                                               cAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAatat                      <  2:1299767/150‑1 (MQ=255)
                                                                                                                                aaGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAtata                     >  1:842888/1‑150 (MQ=255)
                                                                                                                                    gcTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACa                 <  2:1148873/150‑1 (MQ=255)
                                                                                                                                    gcTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACa                 >  1:1406477/1‑150 (MQ=255)
                                                                                                                                     cTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACaa                <  1:2676692/150‑1 (MQ=255)
                                                                                                                                     cTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACaa                >  2:2089252/1‑150 (MQ=255)
                                                                                                                                      tGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAt               >  2:3369278/1‑150 (MQ=255)
                                                                                                                                       gAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAtt              <  2:731119/150‑1 (MQ=255)
                                                                                                                                        aaCCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTa             <  1:1175819/150‑1 (MQ=255)
                                                                                                                                          ccATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc           <  1:2485463/150‑1 (MQ=255)
                                                                                                                                          ccATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc           <  1:3004282/150‑1 (MQ=255)
                                                                                                                                             tatGCGATAAGGAAGAAGCGACTTAGTTAGTAATACCAAAAACAATAACAAAAATATACAACAACAAATGAGATTTATAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACAAATATTAACAATTATcaac        <  2:346636/150‑1 (MQ=255)
                                                                                                                                                gcgaTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAAt     <  1:3394583/150‑1 (MQ=255)
                                                                                                                                                   cTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGtt  >  2:1482217/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314723‑3315019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314721‑3315019
                                                                                                                                                     |                                                                                                                                                     
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGA                                                                                                                                                       <  SRR19973074.3518974/150‑1 (MQ=60)
 GTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGAT                                                                                                                                                      >  SRR19973074.1971883/1‑150 (MQ=60)
 GTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGAT                                                                                                                                                      <  SRR19973074.1152720/150‑1 (MQ=60)
  TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATC                                                                                                                                                     <  SRR19973074.3162549/150‑1 (MQ=60)
     ACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGG                                                                                                                                                  >  SRR19973074.269854/1‑150 (MQ=60)
       CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                >  SRR19973074.924890/1‑150 (MQ=60)
       CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                >  SRR19973074.3372850/1‑150 (MQ=60)
       CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                >  SRR19973074.3372929/1‑150 (MQ=60)
       CATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAA                                                                                                                                                <  SRR19973074.627038/150‑1 (MQ=60)
          GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACACGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                             <  SRR19973074.2578856/150‑1 (MQ=60)
          GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                             >  SRR19973074.732492/1‑150 (MQ=60)
          GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                             <  SRR19973074.1856606/150‑1 (MQ=60)
            ATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGC                                                                                                                                           <  SRR19973074.855700/150‑1 (MQ=60)
                    TAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGT                                                                                                                                   >  SRR19973074.3229666/1‑150 (MQ=60)
                      ATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTC                                                                                                                                 <  SRR19973074.3443227/150‑1 (MQ=60)
                      ATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTC                                                                                                                                 <  SRR19973074.819196/150‑1 (MQ=60)
                       TACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCG                                                                                                                                <  SRR19973074.165448/150‑1 (MQ=60)
                        ACTCATTTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGT                                                                                                                               >  SRR19973074.1992316/1‑150 (MQ=60)
                        ACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGT                                                                                                                               >  SRR19973074.2927370/1‑150 (MQ=60)
                         CTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTA                                                                                                                              <  SRR19973074.2401891/150‑1 (MQ=60)
                          TCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGCCTAAGTTCGTAA                                                                                                                             >  SRR19973074.3337095/1‑150 (MQ=60)
                          TCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAA                                                                                                                             >  SRR19973074.3817792/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            >  SRR19973074.15853/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            >  SRR19973074.15854/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            >  SRR19973074.302977/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            >  SRR19973074.15659/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                            >  SRR19973074.1571377/1‑150 (MQ=60)
                            ATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATA                                                                                                                           <  SRR19973074.1683987/150‑1 (MQ=60)
                            ATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATA                                                                                                                           >  SRR19973074.1031078/1‑150 (MQ=60)
                                 TGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAA                                                                                                                      >  SRR19973074.2047263/1‑150 (MQ=60)
                                        GTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATA                                                                                                               <  SRR19973074.3146881/150‑1 (MQ=60)
                                           AGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACA                                                                                                            >  SRR19973074.2035613/1‑150 (MQ=60)
                                              AACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAA                                                                                                         >  SRR19973074.3456041/1‑150 (MQ=60)
                                              AACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAA                                                                                                         >  SRR19973074.1282097/1‑150 (MQ=60)
                                              AACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAA                                                                                                         >  SRR19973074.2060125/1‑150 (MQ=60)
                                              AACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAA                                                                                                         >  SRR19973074.1282252/1‑150 (MQ=60)
                                                 ACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGA                                                                                                      >  SRR19973074.2654224/1‑150 (MQ=60)
                                                   AAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATA                                                                                                    >  SRR19973074.3238628/1‑150 (MQ=60)
                                                            GGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCA                                                                                           <  SRR19973074.916310/150‑1 (MQ=60)
                                                            GGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCA                                                                                           <  SRR19973074.1550102/150‑1 (MQ=60)
                                                             GCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAA                                                                                          >  SRR19973074.1822418/1‑150 (MQ=60)
                                                              CAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAAT                                                                                         >  SRR19973074.3211712/1‑150 (MQ=60)
                                                              CAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAAT                                                                                         <  SRR19973074.1073414/150‑1 (MQ=60)
                                                               AACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATG                                                                                        >  SRR19973074.3176646/1‑150 (MQ=60)
                                                               AACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATG                                                                                        >  SRR19973074.253093/1‑150 (MQ=60)
                                                                 CAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAG                                                                                      <  SRR19973074.2396895/150‑1 (MQ=60)
                                                                 CAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAG                                                                                      <  SRR19973074.2396920/150‑1 (MQ=60)
                                                                  AAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGA                                                                                     >  SRR19973074.3705742/1‑150 (MQ=60)
                                                                   AGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAT                                                                                    <  SRR19973074.3390250/150‑1 (MQ=60)
                                                                    GCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATT                                                                                   >  SRR19973074.2180595/1‑150 (MQ=60)
                                                                    GCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATT                                                                                   >  SRR19973074.2180752/1‑150 (MQ=60)
                                                                    GCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATT                                                                                   >  SRR19973074.1336333/1‑150 (MQ=60)
                                                                     CAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTT                                                                                  >  SRR19973074.4015725/1‑150 (MQ=60)
                                                                      AGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTA                                                                                 >  SRR19973074.2506360/1‑150 (MQ=60)
                                                                         CGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAA                                                                              >  SRR19973074.1154788/1‑150 (MQ=60)
                                                                               GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                        <  SRR19973074.3285271/150‑1 (MQ=60)
                                                                                 AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      >  SRR19973074.258632/1‑150 (MQ=60)
                                                                                 AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      >  SRR19973074.2188180/1‑150 (MQ=60)
                                                                                 AAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGC                                                                      >  SRR19973074.593083/1‑150 (MQ=60)
                                                                                  AGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCA                                                                     <  SRR19973074.307540/150‑1 (MQ=60)
                                                                                   GGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAA                                                                    >  SRR19973074.580350/1‑150 (MQ=60)
                                                                                   GGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAA                                                                    >  SRR19973074.580385/1‑150 (MQ=60)
                                                                                       TGATATACGTTTTACAACCAGGATTGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTT                                                                >  SRR19973074.721631/1‑150 (MQ=60)
                                                                                       TGATATACGTTTTACAACCAGGATTGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTT                                                                >  SRR19973074.721609/1‑150 (MQ=60)
                                                                                       TGATATACGTTTTACAACCAGGATTGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTT                                                                >  SRR19973074.721626/1‑150 (MQ=60)
                                                                                         ATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGT                                                              <  SRR19973074.269521/150‑1 (MQ=60)
                                                                                           ATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTG                                                            <  SRR19973074.2961367/150‑1 (MQ=60)
                                                                                           ATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTG                                                            >  SRR19973074.2615781/1‑150 (MQ=60)
                                                                                           ATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTG                                                            >  SRR19973074.2615771/1‑150 (MQ=60)
                                                                                           ATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTG                                                            >  SRR19973074.1331629/1‑150 (MQ=60)
                                                                                              CGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAAT                                                         <  SRR19973074.2948718/150‑1 (MQ=60)
                                                                                              CGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAAT                                                         <  SRR19973074.549480/150‑1 (MQ=60)
                                                                                               GTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATA                                                        <  SRR19973074.2946256/150‑1 (MQ=60)
                                                                                                  TTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTG                                                     >  SRR19973074.3342370/1‑150 (MQ=60)
                                                                                                       ACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATC                                                >  SRR19973074.2289880/1‑150 (MQ=60)
                                                                                                       ACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATC                                                >  SRR19973074.204552/1‑150 (MQ=60)
                                                                                                         CAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCA                                              >  SRR19973074.1209283/1‑150 (MQ=60)
                                                                                                          AGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAG                                             >  SRR19973074.1882338/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGANCCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973074.3859702/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGANCCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973074.3859709/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973074.634739/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973074.634803/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973074.634748/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                          >  SRR19973074.634732/1‑150 (MQ=60)
                                                                                                               GGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATC                                        <  SRR19973074.1450734/150‑1 (MQ=60)
                                                                                                               GGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATC                                        <  SRR19973074.1197101/150‑1 (MQ=60)
                                                                                                               GGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATC                                        >  SRR19973074.2213873/1‑150 (MQ=60)
                                                                                                               GGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATC                                        >  SRR19973074.2125232/1‑150 (MQ=60)
                                                                                                                  CAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAA                                     >  SRR19973074.3921870/1‑150 (MQ=60)
                                                                                                                  CAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAA                                     >  SRR19973074.3712392/1‑150 (MQ=60)
                                                                                                                     AACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTT                                  <  SRR19973074.3305819/150‑1 (MQ=60)
                                                                                                                     AACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTT                                  <  SRR19973074.3305763/150‑1 (MQ=60)
                                                                                                                      ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                 <  SRR19973074.3616520/150‑1 (MQ=60)
                                                                                                                      ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                 <  SRR19973074.2065510/150‑1 (MQ=60)
                                                                                                                         AAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACA                              >  SRR19973074.1284532/1‑150 (MQ=60)
                                                                                                                          AGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAA                             <  SRR19973074.3613686/150‑1 (MQ=60)
                                                                                                                             ACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCA                          <  SRR19973074.2988155/150‑1 (MQ=60)
                                                                                                                                  AAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATA                     >  SRR19973074.844515/1‑150 (MQ=60)
                                                                                                                                     CGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCATAAACAATAACACAAAGATACAACATCCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAAC                  <  SRR19973074.2254543/150‑1 (MQ=60)
                                                                                                                                     CGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAAC                  <  SRR19973074.3630847/150‑1 (MQ=60)
                                                                                                                                      GCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACA                 >  SRR19973074.1409069/1‑150 (MQ=60)
                                                                                                                                       CTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAA                <  SRR19973074.2681674/150‑1 (MQ=60)
                                                                                                                                          AACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTA             <  SRR19973074.1178059/150‑1 (MQ=60)
                                                                                                                                          AACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTA             <  SRR19973074.1178049/150‑1 (MQ=60)
                                                                                                                                           ACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTAT            >  SRR19973074.2458688/1‑150 (MQ=60)
                                                                                                                                            CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973074.1412387/150‑1 (MQ=60)
                                                                                                                                            CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973074.2490080/150‑1 (MQ=60)
                                                                                                                                            CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973074.2490264/150‑1 (MQ=60)
                                                                                                                                            CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC           <  SRR19973074.3009855/150‑1 (MQ=60)
                                                                                                                                             CATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCA          <  SRR19973074.2667187/150‑1 (MQ=60)
                                                                                                                                                ATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGATGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACA       >  SRR19973074.840390/1‑150 (MQ=60)
                                                                                                                                                  GCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAAT     <  SRR19973074.3400867/150‑1 (MQ=60)
                                                                                                                                                    GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT   >  SRR19973074.38439/1‑150 (MQ=60)
                                                                                                                                                    GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT   >  SRR19973074.1546341/1‑150 (MQ=60)
                                                                                                                                                    GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAAGGT   >  SRR19973074.1546367/1‑150 (MQ=60)
                                                                                                                                                     ATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  <  SRR19973074.50784/150‑1 (MQ=60)
                                                                                                                                                     ATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  <  SRR19973074.3553440/150‑1 (MQ=60)
                                                                                                                                                     ATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  <  SRR19973074.3553437/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTT  >  CP009273/3314721‑3315019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑