Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,314,870 C→A 100% A35S (GCG→TCG)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,314,8700CA96.0% 647.9 / 17.4 201A35S (GCG→TCG) yhbXputative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (86/107);  total (90/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAA  >  CP009273/3314723‑3315015
                                                                                                                                                   |                                                                                                                                                 
tACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATc                                                                                                                                                 <  2:2279632/150‑1 (MQ=255)
  cacCATGAATTGAGGCTAATACACATGTCTGCCGCTGTCAGTAACACAAATGTTGCGGCATCTAGCAGGCGAAGCGGAAGGGATTATATACGTTTTACAACCAGGTTGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCg                                                                                                                                               <  2:2729741/150‑1 (MQ=255)
    ccATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGga                                                                                                                                             >  2:3545474/1‑150 (MQ=255)
    ccATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGga                                                                                                                                             >  1:2818714/1‑150 (MQ=255)
    ccATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGga                                                                                                                                             >  1:4515900/1‑150 (MQ=255)
       tGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaaga                                                                                                                                          >  1:461016/1‑150 (MQ=255)
        gAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGgaagaa                                                                                                                                         >  1:750095/1‑150 (MQ=255)
         aaTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGaagaag                                                                                                                                        >  1:2926828/1‑150 (MQ=255)
          aTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGc                                                                                                                                       >  2:1795649/1‑150 (MQ=255)
          aTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGc                                                                                                                                       <  1:4210514/150‑1 (MQ=255)
            agagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGa                                                                                                                                     <  1:2341836/150‑1 (MQ=255)
            agagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGa                                                                                                                                     <  1:205129/150‑1 (MQ=255)
             gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGAc                                                                                                                                    <  1:1407375/150‑1 (MQ=255)
              agGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACt                                                                                                                                   <  1:3727586/150‑1 (MQ=255)
                gCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTaa                                                                                                                                 <  1:1955453/150‑1 (MQ=255)
                   aaTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGtt                                                                                                                              >  2:3673577/1‑150 (MQ=255)
                   aaTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGtt                                                                                                                              >  2:2161396/1‑150 (MQ=255)
                    aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTc                                                                                                                             <  2:3507111/150‑1 (MQ=255)
                       cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTa                                                                                                                          <  2:1862737/150‑1 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                        >  1:1345550/1‑150 (MQ=255)
                         cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAt                                                                                                                        <  1:3767075/150‑1 (MQ=255)
                          aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATa                                                                                                                       >  1:2184067/1‑150 (MQ=255)
                            gTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCTGGCGAAGCGGAAGGGATGATATACTTTTTAAAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATAcc                                                                                                                     <  1:1197546/150‑1 (MQ=255)
                            gTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATAcc                                                                                                                     <  2:1521606/150‑1 (MQ=255)
                             tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCa                                                                                                                    <  1:2597704/150‑1 (MQ=255)
                             tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCa                                                                                                                    <  1:1616883/150‑1 (MQ=255)
                              cTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCaa                                                                                                                   <  1:1050988/150‑1 (MQ=255)
                               tGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCaaa                                                                                                                  <  2:4565609/150‑1 (MQ=255)
                                  cGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAAc                                                                                                               >  1:3076695/1‑150 (MQ=255)
                                    cagtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACaa                                                                                                             >  1:2586304/1‑150 (MQ=255)
                                     agtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAAt                                                                                                            <  1:94514/150‑1 (MQ=255)
                                     agtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAAt                                                                                                            <  1:3321287/150‑1 (MQ=255)
                                      gtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGATTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATa                                                                                                           >  2:3599202/1‑150 (MQ=255)
                                         agtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAaca                                                                                                        <  2:3012275/150‑1 (MQ=255)
                                           tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAAcaca                                                                                                      >  2:4433053/1‑150 (MQ=255)
                                               acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGa                                                                                                  >  1:3184406/1‑150 (MQ=255)
                                                caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGAt                                                                                                 <  1:1463299/150‑1 (MQ=255)
                                                  actGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATac                                                                                               <  2:2028957/148‑1 (MQ=255)
                                                   aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATaca                                                                                              <  2:1466942/150‑1 (MQ=255)
                                                     ggTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATacaac                                                                                            <  2:1054127/150‑1 (MQ=255)
                                                       tGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATAcaacaa                                                                                          <  1:4577726/150‑1 (MQ=255)
                                                        gCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATNCCAAAAACAATAACACAAAGATACaacaac                                                                                         <  1:4281988/150‑1 (MQ=255)
                                                        gCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACaacaac                                                                                         <  2:2943238/150‑1 (MQ=255)
                                                        gCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACaacaac                                                                                         <  1:1698160/150‑1 (MQ=255)
                                                         cGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAAcc                                                                                        <  2:1995756/150‑1 (MQ=255)
                                                           gCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCaa                                                                                      >  2:1113679/1‑150 (MQ=255)
                                                             aacaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATg                                                                                    >  2:127801/1‑150 (MQ=255)
                                                              acaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATga                                                                                   <  2:1958876/150‑1 (MQ=255)
                                                              acaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATga                                                                                   <  1:1281625/150‑1 (MQ=255)
                                                               caaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATgag                                                                                  <  2:1395674/150‑1 (MQ=255)
                                                                aaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATgaga                                                                                 >  2:2652455/1‑150 (MQ=255)
                                                                  gCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAtt                                                                               <  1:2999191/150‑1 (MQ=255)
                                                                   cAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAttt                                                                              >  1:186159/1‑150 (MQ=255)
                                                                     ggCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTaa                                                                            <  1:2768023/150‑1 (MQ=255)
                                                                        gAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAg                                                                         >  1:570453/1‑150 (MQ=255)
                                                                         aaGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGt                                                                        >  1:1670049/1‑150 (MQ=255)
                                                                          aGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGtt                                                                       <  1:4347727/150‑1 (MQ=255)
                                                                           gCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTc                                                                      <  2:2589984/150‑1 (MQ=255)
                                                                             ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                    >  1:4542688/1‑150 (MQ=255)
                                                                             ggAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTa                                                                    >  1:1899760/1‑150 (MQ=255)
                                                                              gAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAg                                                                   <  1:965580/150‑1 (MQ=255)
                                                                                aGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCa                                                                 <  2:409000/150‑1 (MQ=255)
                                                                                    atgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAtt                                                             >  1:1150229/1‑150 (MQ=255)
                                                                                     tgatATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAttt                                                            <  1:2118105/150‑1 (MQ=255)
                                                                                        tataCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATttgtt                                                         <  2:2354682/150‑1 (MQ=255)
                                                                                          taCGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGa                                                       >  1:311012/1‑150 (MQ=255)
                                                                                             gTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATa                                                    >  1:1534355/1‑150 (MQ=255)
                                                                                              ttttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCCAATTTGTTGAATAc                                                   >  2:277472/1‑150 (MQ=255)
                                                                                               tttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACt                                                  >  1:1465756/1‑150 (MQ=255)
                                                                                               tttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACt                                                  <  2:346599/150‑1 (MQ=255)
                                                                                                ttACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTg                                                 <  2:583924/150‑1 (MQ=255)
                                                                                                 tACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGt                                                >  1:1751737/1‑150 (MQ=255)
                                                                                                 tACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGt                                                <  2:2554027/150‑1 (MQ=255)
                                                                                                     aCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATc                                            >  1:2376218/1‑150 (MQ=255)
                                                                                                     aCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATc                                            >  1:2376252/1‑150 (MQ=255)
                                                                                                        aGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAg                                         >  1:3207343/1‑150 (MQ=255)
                                                                                                        aGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAg                                         >  1:3886833/1‑150 (MQ=255)
                                                                                                               tCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCa                                  <  1:1831725/150‑1 (MQ=255)
                                                                                                               tCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCa                                  <  1:4380390/150‑1 (MQ=255)
                                                                                                                 aGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAg                                >  1:879517/1‑150 (MQ=255)
                                                                                                                   aaCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGtt                              <  2:1936686/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                             <  2:2498307/150‑1 (MQ=255)
                                                                                                                    aCGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTc                             <  1:1638769/150‑1 (MQ=255)
                                                                                                                      gaagaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTcac                           <  2:2377978/150‑1 (MQ=255)
                                                                                                                       aagaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTcaca                          >  1:1011087/1‑150 (MQ=255)
                                                                                                                       aagaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTcaca                          <  2:2118643/150‑1 (MQ=255)
                                                                                                                         gaaCAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAc                        <  2:4155373/150‑1 (MQ=255)
                                                                                                                                aaGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAtata                 <  1:1999426/150‑1 (MQ=255)
                                                                                                                                 aGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATaa                >  1:536958/1‑150 (MQ=255)
                                                                                                                                 aGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATaa                >  1:4006560/1‑150 (MQ=255)
                                                                                                                                    gcTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACa             >  1:3942469/1‑150 (MQ=255)
                                                                                                                                      tGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAt           <  2:1589252/150‑1 (MQ=255)
                                                                                                                                       gAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAtt          <  1:3455397/150‑1 (MQ=255)
                                                                                                                                       gAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAtt          <  1:2286478/150‑1 (MQ=255)
                                                                                                                                          ccATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACACCCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc       <  2:136463/150‑1 (MQ=255)
                                                                                                                                          ccATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATc       >  1:2439525/1‑150 (MQ=255)
                                                                                                                                            atatGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACACTTATcaa     >  2:2344488/1‑150 (MQ=255)
                                                                                                                                            atatGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaa     >  1:1357467/1‑150 (MQ=255)
                                                                                                                                            atatGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTACTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaa     >  1:3807473/1‑150 (MQ=255)
                                                                                                                                               tGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATcaacaa  >  2:1417238/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAA  >  CP009273/3314723‑3315015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT  >  CP009273/3314721‑3315018
                                                                                                                                                     |                                                                                                                                                    
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGA                                                                                                                                                      >  SRR19973073.3514117/1‑150 (MQ=60)
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGA                                                                                                                                                      >  SRR19973073.142919/1‑150 (MQ=60)
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGA                                                                                                                                                      <  SRR19973073.2313581/150‑1 (MQ=60)
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGA                                                                                                                                                      <  SRR19973073.2313750/150‑1 (MQ=60)
    CACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCG                                                                                                                                                  >  SRR19973073.1312952/1‑150 (MQ=60)
      CCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGA                                                                                                                                                >  SRR19973073.4524969/1‑150 (MQ=60)
      CCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGA                                                                                                                                                >  SRR19973073.2824495/1‑150 (MQ=60)
        ATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAG                                                                                                                                              >  SRR19973073.3260789/1‑150 (MQ=60)
         TGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGA                                                                                                                                             >  SRR19973073.461969/1‑150 (MQ=60)
          GAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAA                                                                                                                                            >  SRR19973073.751613/1‑150 (MQ=60)
           AATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAG                                                                                                                                           >  SRR19973073.2932812/1‑150 (MQ=60)
            ATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGC                                                                                                                                          <  SRR19973073.4218872/150‑1 (MQ=60)
              AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGA                                                                                                                                        <  SRR19973073.2346624/150‑1 (MQ=60)
              AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGA                                                                                                                                        <  SRR19973073.205609/150‑1 (MQ=60)
              AGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGA                                                                                                                                        >  SRR19973073.3965992/1‑150 (MQ=60)
               GAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGAC                                                                                                                                       <  SRR19973073.1410349/150‑1 (MQ=60)
                AGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACT                                                                                                                                      >  SRR19973073.4308504/1‑150 (MQ=60)
                AGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACT                                                                                                                                      <  SRR19973073.3735078/150‑1 (MQ=60)
                AGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACT                                                                                                                                      <  SRR19973073.3735070/150‑1 (MQ=60)
                  GCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAA                                                                                                                                    <  SRR19973073.1959478/150‑1 (MQ=60)
                  GCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAA                                                                                                                                    <  SRR19973073.1959522/150‑1 (MQ=60)
                   CTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAG                                                                                                                                   <  SRR19973073.1853489/150‑1 (MQ=60)
                      ATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTC                                                                                                                                >  SRR19973073.2157287/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                           <  SRR19973073.4551236/150‑1 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                           <  SRR19973073.3774625/150‑1 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                           >  SRR19973073.1348409/1‑150 (MQ=60)
                           CATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAAT                                                                                                                           <  SRR19973073.1834348/150‑1 (MQ=60)
                            ATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATA                                                                                                                          >  SRR19973073.2188624/1‑150 (MQ=60)
                              GTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCTGGCGAAGCGGAAGGGATGATATACTTTTTAAAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACC                                                                                                                        <  SRR19973073.1200029/150‑1 (MQ=60)
                               TCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCA                                                                                                                       <  SRR19973073.1620216/150‑1 (MQ=60)
                               TCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCA                                                                                                                       <  SRR19973073.2603048/150‑1 (MQ=60)
                                CTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAA                                                                                                                      <  SRR19973073.1053174/150‑1 (MQ=60)
                                 TGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAA                                                                                                                     <  SRR19973073.1734803/150‑1 (MQ=60)
                                    CGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAAC                                                                                                                  >  SRR19973073.3082896/1‑150 (MQ=60)
                                    CGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAAC                                                                                                                  >  SRR19973073.3082875/1‑150 (MQ=60)
                                      CAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAA                                                                                                                >  SRR19973073.2591623/1‑150 (MQ=60)
                                       AGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAAT                                                                                                               <  SRR19973073.3328005/150‑1 (MQ=60)
                                       AGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAAT                                                                                                               <  SRR19973073.94751/150‑1 (MQ=60)
                                       AGTCAGTAACACAAATGGTGCGGCAACAAGCAGGAGAAGCGGAAGGGATGATATACGTTTTACAACCATGATGTTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAAT                                                                                                               <  SRR19973073.94748/150‑1 (MQ=60)
                                         TCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAA                                                                                                             >  SRR19973073.2601070/1‑150 (MQ=60)
                                            GTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACAC                                                                                                          >  SRR19973073.2471717/1‑150 (MQ=60)
                                                 ACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGA                                                                                                     >  SRR19973073.3190829/1‑150 (MQ=60)
                                                  CAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGAT                                                                                                    <  SRR19973073.1466365/150‑1 (MQ=60)
                                                  CAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGAT                                                                                                    <  SRR19973073.1466399/150‑1 (MQ=60)
                                                         TGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAA                                                                                             <  SRR19973073.4586918/150‑1 (MQ=60)
                                                         TGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAA                                                                                             <  SRR19973073.4586878/150‑1 (MQ=60)
                                                          GCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATNCCAAAAACAATAACACAAAGATACAACAAC                                                                                            <  SRR19973073.4290492/150‑1 (MQ=60)
                                                          GCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAAC                                                                                            <  SRR19973073.1701682/150‑1 (MQ=60)
                                                             GCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAA                                                                                         <  SRR19973073.2018422/150‑1 (MQ=60)
                                                             GCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAA                                                                                         <  SRR19973073.3366286/150‑1 (MQ=60)
                                                              CAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAAT                                                                                        <  SRR19973073.2988464/150‑1 (MQ=60)
                                                              CAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAAT                                                                                        <  SRR19973073.2365857/150‑1 (MQ=60)
                                                               AACAAGCAGGCGAAGTGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATG                                                                                       <  SRR19973073.2120972/150‑1 (MQ=60)
                                                                ACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGA                                                                                      <  SRR19973073.1284315/150‑1 (MQ=60)
                                                                   AGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAT                                                                                   <  SRR19973073.4584283/150‑1 (MQ=60)
                                                                   AGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGAT                                                                                   <  SRR19973073.714329/150‑1 (MQ=60)
                                                                    GCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATT                                                                                  <  SRR19973073.3005280/150‑1 (MQ=60)
                                                                    GCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATT                                                                                  <  SRR19973073.785172/150‑1 (MQ=60)
                                                                     CAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTT                                                                                 >  SRR19973073.186599/1‑150 (MQ=60)
                                                                     CAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTT                                                                                 <  SRR19973073.1221566/150‑1 (MQ=60)
                                                                       GGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAA                                                                               <  SRR19973073.2773688/150‑1 (MQ=60)
                                                                       GGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAA                                                                               <  SRR19973073.2773705/150‑1 (MQ=60)
                                                                       GGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAA                                                                               <  SRR19973073.1454712/150‑1 (MQ=60)
                                                                          GAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAG                                                                            >  SRR19973073.571678/1‑150 (MQ=60)
                                                                          GAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAG                                                                            >  SRR19973073.4407200/1‑150 (MQ=60)
                                                                           AAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGT                                                                           >  SRR19973073.1673518/1‑150 (MQ=60)
                                                                            AGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTT                                                                          <  SRR19973073.4356430/150‑1 (MQ=60)
                                                                               GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                       >  SRR19973073.1903633/1‑150 (MQ=60)
                                                                               GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                       >  SRR19973073.1903632/1‑150 (MQ=60)
                                                                               GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                       >  SRR19973073.1903499/1‑150 (MQ=60)
                                                                               GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                       >  SRR19973073.4551795/1‑150 (MQ=60)
                                                                               GGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTA                                                                       <  SRR19973073.1541185/150‑1 (MQ=60)
                                                                                GAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAG                                                                      <  SRR19973073.967601/150‑1 (MQ=60)
                                                                                    GGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAA                                                                  >  SRR19973073.4366171/1‑150 (MQ=60)
                                                                                     GATGATATACGTTTTACAACCTGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAAT                                                                 <  SRR19973073.515734/150‑1 (MQ=60)
                                                                                      ATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATT                                                                >  SRR19973073.1152586/1‑150 (MQ=60)
                                                                                      ATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATT                                                                >  SRR19973073.1152556/1‑150 (MQ=60)
                                                                                       TGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTT                                                               <  SRR19973073.2122540/150‑1 (MQ=60)
                                                                                          TATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCACGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCCATGAGATTTAAAAGTTCTAGCAAATTTGTT                                                            >  SRR19973073.540827/1‑150 (MQ=60)
                                                                                            TACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGA                                                          >  SRR19973073.311658/1‑150 (MQ=60)
                                                                                            TACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGA                                                          >  SRR19973073.311660/1‑150 (MQ=60)
                                                                                             ACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAA                                                         <  SRR19973073.1068700/150‑1 (MQ=60)
                                                                                               GTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATA                                                       >  SRR19973073.1537498/1‑150 (MQ=60)
                                                                                                 TTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACT                                                     <  SRR19973073.4252548/150‑1 (MQ=60)
                                                                                                 TTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACT                                                     >  SRR19973073.1468825/1‑150 (MQ=60)
                                                                                                 TTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACT                                                     >  SRR19973073.1469259/1‑150 (MQ=60)
                                                                                                   TACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGT                                                   >  SRR19973073.1755371/1‑150 (MQ=60)
                                                                                                      AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCAT                                                <  SRR19973073.951354/150‑1 (MQ=60)
                                                                                                       ACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATC                                               >  SRR19973073.2381081/1‑150 (MQ=60)
                                                                                                       ACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATC                                               >  SRR19973073.2381095/1‑150 (MQ=60)
                                                                                                       ACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATC                                               >  SRR19973073.2381115/1‑150 (MQ=60)
                                                                                                          AGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAG                                            >  SRR19973073.3213823/1‑150 (MQ=60)
                                                                                                          AGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAG                                            >  SRR19973073.3894546/1‑150 (MQ=60)
                                                                                                            GATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAA                                          >  SRR19973073.2317715/1‑150 (MQ=60)
                                                                                                             ATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAA                                         >  SRR19973073.3074826/1‑150 (MQ=60)
                                                                                                                GTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCC                                      <  SRR19973073.3822154/150‑1 (MQ=60)
                                                                                                                 TCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCA                                     <  SRR19973073.1835502/150‑1 (MQ=60)
                                                                                                                 TCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCA                                     <  SRR19973073.4389177/150‑1 (MQ=60)
                                                                                                                   AGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAG                                   >  SRR19973073.881333/1‑150 (MQ=60)
                                                                                                                   AGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAG                                   >  SRR19973073.3212501/1‑150 (MQ=60)
                                                                                                                   AGAACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAG                                   >  SRR19973073.2666681/1‑150 (MQ=60)
                                                                                                                      ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                <  SRR19973073.2278020/150‑1 (MQ=60)
                                                                                                                      ACGAAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTC                                <  SRR19973073.1642160/150‑1 (MQ=60)
                                                                                                                         AAGAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACA                             >  SRR19973073.1013199/1‑150 (MQ=60)
                                                                                                                           GAACAGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAAC                           >  SRR19973073.2863570/1‑150 (MQ=60)
                                                                                                                               AGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAT                       >  SRR19973073.1676260/1‑150 (MQ=60)
                                                                                                                               AGCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAAT                       <  SRR19973073.3552348/150‑1 (MQ=60)
                                                                                                                                GCAAGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATA                      <  SRR19973073.1388909/150‑1 (MQ=60)
                                                                                                                                  AAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATA                    <  SRR19973073.2003576/150‑1 (MQ=60)
                                                                                                                                  AAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATA                    <  SRR19973073.4128722/150‑1 (MQ=60)
                                                                                                                                   AGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAA                   >  SRR19973073.538118/1‑150 (MQ=60)
                                                                                                                                   AGCGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAA                   >  SRR19973073.4014557/1‑150 (MQ=60)
                                                                                                                                     CGCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAAC                 <  SRR19973073.1627636/150‑1 (MQ=60)
                                                                                                                                      GCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACA                >  SRR19973073.3950321/1‑150 (MQ=60)
                                                                                                                                      GCTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACA                >  SRR19973073.3906773/1‑150 (MQ=60)
                                                                                                                                       CTGAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAA               <  SRR19973073.1031379/150‑1 (MQ=60)
                                                                                                                                         GAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATT             <  SRR19973073.3800413/150‑1 (MQ=60)
                                                                                                                                         GAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATT             <  SRR19973073.2291146/150‑1 (MQ=60)
                                                                                                                                         GAACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATT             <  SRR19973073.3462279/150‑1 (MQ=60)
                                                                                                                                           ACCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTAT           >  SRR19973073.1667942/1‑150 (MQ=60)
                                                                                                                                            CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC          >  SRR19973073.2444470/1‑150 (MQ=60)
                                                                                                                                            CCATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATC          >  SRR19973073.2444574/1‑150 (MQ=60)
                                                                                                                                              ATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAA        >  SRR19973073.1360343/1‑150 (MQ=60)
                                                                                                                                              ATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAA        >  SRR19973073.1360031/1‑150 (MQ=60)
                                                                                                                                              ATATGCGATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTACTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAA        >  SRR19973073.3815067/1‑150 (MQ=60)
                                                                                                                                                    GATCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT  >  SRR19973073.1807158/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGT  >  CP009273/3314721‑3315018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑