Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F54 I0 R1	640	319.5	10493376	95.3%	10000187	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	923,339	G→T	10.4%	S438* (TCG→TAG)	cydC ←	fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	923,339	0	G	T	10.4%	1019.7 / 66.7	295	S438* (TCG→TAG)	cydC	fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand): ref base G (135/129); new base T (20/11); total (155/140)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

CACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATGCG  >  CP009273/923190‑923488

  cGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggcgag                                                                                                                                                     <  2:1478880/150‑1 (MQ=255)

  cGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggcgag                                                                                                                                                     >  1:4011424/1‑150 (MQ=255)

  cGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggcgag                                                                                                                                                     >  1:2075293/1‑150 (MQ=255)

      cGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAggcgaggcga                                                                                                                                                 >  1:1170858/1‑150 (MQ=255)

       gATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGgcgaggcgag                                                                                                                                                >  1:691176/1‑150 (MQ=255)

         tAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTa                                                                                                                                              >  1:4056295/1‑150 (MQ=255)

            ccAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTaaaa                                                                                                                                           >  2:3454407/1‑150 (MQ=255)

              aGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGa                                                                                                                                         <  1:1358845/150‑1 (MQ=255)

              aGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGa                                                                                                                                         <  2:2272431/150‑1 (MQ=255)

                  ggCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTAt                                                                                                                                     >  1:3357446/1‑150 (MQ=255)

                  ggCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTAt                                                                                                                                     >  2:3876225/1‑150 (MQ=255)

                        gTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCTAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCa                                                                                                                               >  1:2206897/1‑150 (MQ=255)

                         ttCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAg                                                                                                                              <  1:3855292/150‑1 (MQ=255)

                            accaccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGt                                                                                                                           >  1:1251722/1‑150 (MQ=255)

                               accGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGgc                                                                                                                        >  1:726025/1‑150 (MQ=255)

                                ccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGgcg                                                                                                                       <  1:4359174/150‑1 (MQ=255)

                                     gagCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTaaa                                                                                                                  >  1:3186132/1‑150 (MQ=255)

                                      agCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAAc                                                                                                                 >  1:1961348/1‑150 (MQ=255)

                                      agCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCCGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAAc                                                                                                                 <  2:1236630/150‑1 (MQ=255)

                                         tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              <  2:1891886/150‑1 (MQ=255)

                                         tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              >  1:2554449/1‑150 (MQ=255)

                                         tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              >  2:596151/1‑150 (MQ=255)

                                         tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              >  2:4127628/1‑150 (MQ=255)

                                         tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              >  2:2978698/1‑150 (MQ=255)

                                             gTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCa                                                                                                          >  2:1423041/1‑150 (MQ=255)

                                             gTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCa                                                                                                          >  1:1918916/1‑150 (MQ=255)

                                              tCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCAc                                                                                                         >  1:99586/1‑150 (MQ=255)

                                              tCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCAc                                                                                                         <  1:4484814/150‑1 (MQ=255)

                                                cGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTc                                                                                                       >  1:2339846/1‑150 (MQ=255)

                                                 gCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCg                                                                                                      >  2:285547/1‑150 (MQ=255)

                                                     tCACCGAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGa                                                                                                  <  1:3242344/150‑1 (MQ=255)

                                                      cACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAg                                                                                                 <  1:516012/150‑1 (MQ=255)

                                                        ccTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGa                                                                                               >  1:3817700/1‑150 (MQ=255)

                                                           aaCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaaca                                                                                            <  2:2545130/150‑1 (MQ=255)

                                                            aCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaa                                                                                           >  1:2650584/1‑150 (MQ=255)

                                                             ccAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaac                                                                                          <  1:4180289/150‑1 (MQ=255)

                                                             ccAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaac                                                                                          <  1:4604049/150‑1 (MQ=255)

                                                               aaCTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGc                                                                                        >  1:3087657/1‑150 (MQ=255)

                                                                   gTTGTGACCTGCTTCCTCGAGCAGCTTTTCCAGGCCATCGCGACGCAAGATCTCCGACAGAGCCTCATCTCTACTGCGCGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAATCAGATGCTCTCGCTGAGGTAGAACGCTGAt                                                                                    <  2:3499663/150‑1 (MQ=255)

                                                                    ttGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATg                                                                                   >  1:2799318/1‑150 (MQ=255)

                                                                      gagaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGt                                                                                 >  2:2618503/1‑150 (MQ=255)

                                                                         aCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTg                                                                              <  1:246863/150‑1 (MQ=255)

                                                                            tGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCg                                                                           >  1:1768147/1‑150 (MQ=255)

                                                                            tGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCg                                                                           <  2:4312030/150‑1 (MQ=255)

                                                                              cATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTa                                                                         >  2:4527131/1‑150 (MQ=255)

                                                                              cATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTa                                                                         >  1:3324319/1‑150 (MQ=255)

                                                                                  cTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGACCAACGCTGATGGTCTGTCGTAGAGc                                                                     >  2:1138575/1‑150 (MQ=255)

                                                                                   tCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGcc                                                                    <  2:5034228/150‑1 (MQ=255)

                                                                                   tCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGcc                                                                    <  1:1091917/150‑1 (MQ=255)

                                                                                         agcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttca                                                              <  2:3716921/150‑1 (MQ=255)

                                                                                              ttCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAg                                                         >  1:2968250/1‑150 (MQ=255)

                                                                                               tCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAgg                                                        <  2:3805409/150‑1 (MQ=255)

                                                                                                ccAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggc                                                       <  2:1708828/150‑1 (MQ=255)

                                                                                                 cAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggct                                                      <  2:4011424/150‑1 (MQ=255)

                                                                                                   ggCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctgg                                                    <  1:4808938/150‑1 (MQ=255)

                                                                                                    gCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctggc                                                   >  2:3591919/1‑150 (MQ=255)

                                                                                                     ccAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctggct                                                  <  2:2595664/150‑1 (MQ=255)

                                                                                                     ccAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctggct                                                  <  2:1674599/150‑1 (MQ=255)

                                                                                                       aaCGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTAt                                                >  2:3891572/1‑150 (MQ=255)

                                                                                                       aaCGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTAt                                                >  2:658829/1‑150 (MQ=255)

                                                                                                         cgcgACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTTGCTATgg                                              <  2:3321284/150‑1 (MQ=255)

                                                                                                            gACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGc                                           <  2:890783/150‑1 (MQ=255)

                                                                                                             aCGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCt                                          <  1:742394/150‑1 (MQ=255)

                                                                                                             aCGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCt                                          <  2:1975796/150‑1 (MQ=255)

                                                                                                              cGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTa                                         >  2:3191276/1‑150 (MQ=255)

                                                                                                              cGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTa                                         <  1:617377/150‑1 (MQ=255)

                                                                                                               gCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTAt                                        >  1:3603969/1‑150 (MQ=255)

                                                                                                                cAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGGAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCAGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATc                                       >  1:1121694/1‑150 (MQ=255)

                                                                                                                 aaGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCg                                      <  1:1750748/150‑1 (MQ=255)

                                                                                                                    aTCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCGATGGGGCTATCGTTa                                   >  1:2719902/1‑150 (MQ=255)

                                                                                                                     tctcCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTaa                                  >  2:1574508/1‑150 (MQ=255)

                                                                                                                     tctcCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTaa                                  >  2:4175986/1‑150 (MQ=255)

                                                                                                                      ctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAg                                 <  2:922072/150‑1 (MQ=255)

                                                                                                                       tcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGc                                >  2:4743067/1‑150 (MQ=255)

                                                                                                                         ctaCAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaa                              <  2:1306906/148‑1 (MQ=255)

                                                                                                                         cGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaa                              >  1:3032615/1‑150 (MQ=255)

                                                                                                                         cGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaa                              >  1:1549789/1‑150 (MQ=255)

                                                                                                                         cGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaa                              >  1:5000632/1‑150 (MQ=255)

                                                                                                                          gACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaa                             <  1:4909296/150‑1 (MQ=255)

                                                                                                                           aCAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaaa                            >  2:5158431/1‑150 (MQ=255)

                                                                                                                            cAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAt                           >  1:5092043/1‑150 (MQ=255)

                                                                                                                             agagCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtc                          <  1:5201028/150‑1 (MQ=255)

                                                                                                                             agagCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtc                          <  1:897545/150‑1 (MQ=255)

                                                                                                                             agagCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCGATCGTTAAGCACAAtc                          >  2:4943435/1‑150 (MQ=255)

                                                                                                                              gagCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtct                         <  1:5139790/150‑1 (MQ=255)

                                                                                                                                 ccTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGc                      <  1:2174897/150‑1 (MQ=255)

                                                                                                                                  cTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGcc                     >  2:2119722/1‑150 (MQ=255)

                                                                                                                                  cTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGcc                     <  1:1692784/150‑1 (MQ=255)

                                                                                                                                     atcaCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTg                  <  2:2424791/150‑1 (MQ=255)

                                                                                                                                     atcaCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTg                  >  1:1496914/1‑150 (MQ=255)

                                                                                                                                        actactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTg               >  2:1925822/1‑150 (MQ=255)

                                                                                                                                            ctGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCggg           <  2:1842341/150‑1 (MQ=255)

                                                                                                                                              gCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTc         >  1:2467723/1‑150 (MQ=255)

                                                                                                                                                cAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTccc       >  2:4464743/1‑150 (MQ=255)

                                                                                                                                                cAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTccc       >  1:373468/1‑150 (MQ=255)

                                                                                                                                                  ggcgaggcgaGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCAt     <  2:2136612/150‑1 (MQ=255)

                                                                                                                                                   gcgaggcgagTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATg    >  2:4413515/1‑150 (MQ=255)

                                                                                                                                                    cgaggcgagTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATgc   >  2:4392759/1‑150 (MQ=255)

                                                                                                                                                    cgaggcgagTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATgc   <  1:805962/150‑1 (MQ=255)

                                                                                                                                                    cgaggcgagTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATgc   >  1:3902606/1‑150 (MQ=255)

CACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATGCG  >  CP009273/923190‑923488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A