Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 923,339 G→T 19.0% S438* (TCG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273923,3390GT19.0% 847.7 / 148.7 297S438* (TCG→TAG) cydCfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (118/122);  new base T (27/30);  total (145/152)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

CACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATGCG  >  CP009273/923190‑923488
                                                                                                                                                     |                                                                                                                                                     
cACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggcg                                                                                                                                                       <  1:2046850/150‑1 (MQ=255)
    ggCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggctaggc                                                                                                                                                   >  1:4191471/1‑150 (MQ=255)
     gCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCaggcgaggcg                                                                                                                                                  <  1:3197772/150‑1 (MQ=255)
      cGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAggcgaggcga                                                                                                                                                 <  1:2426610/150‑1 (MQ=255)
       gATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTATTCCAGGCCAACGCGACGAAAGATCTCCGACAGACCATCATCACAACAGACAGgcgaggccag                                                                                                                                                >  2:3045507/1‑150 (MQ=255)
         tAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTa                                                                                                                                              >  2:1184632/1‑150 (MQ=255)
         tAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTa                                                                                                                                              <  2:1575166/150‑1 (MQ=255)
         tAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTa                                                                                                                                              <  1:5309481/150‑1 (MQ=255)
          aGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTACCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTaa                                                                                                                                             <  1:4575602/150‑1 (MQ=255)
             cAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAg                                                                                                                                          >  1:830511/1‑150 (MQ=255)
              aGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGa                                                                                                                                         >  1:609295/1‑150 (MQ=255)
               gACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGAt                                                                                                                                        <  2:2745377/150‑1 (MQ=255)
                   gcgcAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATc                                                                                                                                    <  1:2829142/150‑1 (MQ=255)
                    cgcAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCa                                                                                                                                   <  1:4339955/150‑1 (MQ=255)
                      cAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACg                                                                                                                                 >  1:3581643/1‑150 (MQ=255)
                         ttCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAg                                                                                                                              <  1:4278709/150‑1 (MQ=255)
                          tCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGc                                                                                                                             >  1:2282134/1‑150 (MQ=255)
                          tCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGc                                                                                                                             >  2:5374103/1‑150 (MQ=255)
                                 cGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGgcgc                                                                                                                      <  1:3175083/150‑1 (MQ=255)
                                      agCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCCACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAAc                                                                                                                 <  2:2677083/150‑1 (MQ=255)
                                       gCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACa                                                                                                                <  1:2366847/150‑1 (MQ=255)
                                       gCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACa                                                                                                                <  1:4005116/150‑1 (MQ=255)
                                         tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGAAAAAGATTATCACGCAGCGTGTCGCTAAACAGa                                                                                                              >  2:4627874/1‑150 (MQ=255)
                                          ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGAt                                                                                                             >  1:5473054/1‑150 (MQ=255)
                                           gCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATg                                                                                                            <  1:3041763/150‑1 (MQ=255)
                                           gCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATg                                                                                                            >  2:4798291/1‑150 (MQ=255)
                                               ccGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACt                                                                                                        >  2:1286738/1‑150 (MQ=255)
                                                cGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTc                                                                                                       >  2:3122857/1‑150 (MQ=255)
                                                 gCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCg                                                                                                      >  1:738117/1‑150 (MQ=255)
                                                   cTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCt                                                                                                    <  2:526401/150‑1 (MQ=255)
                                                     tCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGa                                                                                                  >  2:3625327/1‑150 (MQ=255)
                                                      cACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAg                                                                                                 <  2:3217812/150‑1 (MQ=255)
                                                         cTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaa                                                                                              >  1:5229684/1‑150 (MQ=255)
                                                          tAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaac                                                                                             >  1:5612240/1‑150 (MQ=255)
                                                          tAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaac                                                                                             <  1:664027/150‑1 (MQ=255)
                                                           aaCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaaca                                                                                            <  2:1500033/150‑1 (MQ=255)
                                                            aCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaa                                                                                           >  2:2947730/1‑150 (MQ=255)
                                                             ccAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaac                                                                                          <  1:3841367/150‑1 (MQ=255)
                                                              cAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACg                                                                                         <  1:1401190/150‑1 (MQ=255)
                                                              cAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACg                                                                                         <  1:3536674/150‑1 (MQ=255)
                                                                aCTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCt                                                                                       >  1:3817493/1‑150 (MQ=255)
                                                                 cTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTg                                                                                      <  1:3884753/150‑1 (MQ=255)
                                                                 cTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTg                                                                                      <  2:4386807/150‑1 (MQ=255)
                                                                 cTGTTGAGAACTGCATACTCGAGCAGCTTTTCCAGGCCAACGCGTCGCAAGATCTCCGACAGATCCTCATCACTACTGCCAGGCGATGAGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTAGCTGAGGAACAACGCTg                                                                                      <  2:2004836/150‑1 (MQ=255)
                                                                   gTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGAt                                                                                    <  1:1489371/150‑1 (MQ=255)
                                                                      gagaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGt                                                                                 <  2:3500892/150‑1 (MQ=255)
                                                                        gaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCt                                                                               <  2:657498/150‑1 (MQ=255)
                                                                         aCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTg                                                                              >  1:5531457/1‑150 (MQ=255)
                                                                           cTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTc                                                                            <  1:4072283/150‑1 (MQ=255)
                                                                           cTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTc                                                                            <  1:2738364/150‑1 (MQ=255)
                                                                           cTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGACTGTc                                                                            >  2:3368263/1‑150 (MQ=255)
                                                                              cATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTa                                                                         <  2:4040160/150‑1 (MQ=255)
                                                                               aTCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTag                                                                        >  2:3775338/1‑150 (MQ=255)
                                                                                tCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTaga                                                                       >  1:2559312/1‑150 (MQ=255)
                                                                                  cTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGc                                                                     >  1:3449638/1‑150 (MQ=255)
                                                                                     gAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGc                                                                  >  2:4438763/1‑150 (MQ=255)
                                                                                        cagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttc                                                               >  1:4849523/1‑150 (MQ=255)
                                                                                        cagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttc                                                               >  1:5056334/1‑150 (MQ=255)
                                                                                         agcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttca                                                              <  1:586649/150‑1 (MQ=255)
                                                                                          gcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAACCAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttcat                                                             <  2:1359382/150‑1 (MQ=255)
                                                                                           cTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttcatt                                                            <  1:2094790/150‑1 (MQ=255)
                                                                                                ccAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggc                                                       <  1:3027367/150‑1 (MQ=255)
                                                                                                    gCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctggc                                                   >  1:3284944/1‑150 (MQ=255)
                                                                                                      cAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTa                                                 >  2:790135/1‑150 (MQ=255)
                                                                                                      cAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGAAGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTATAGACGCTAAATTCAGGCTGTCTa                                                 >  2:2895182/1‑150 (MQ=255)
                                                                                                       aaCGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTAt                                                <  1:4163859/150‑1 (MQ=255)
                                                                                                       aaCGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTAt                                                <  1:1848646/150‑1 (MQ=255)
                                                                                                         cgcgACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATgg                                              >  1:1238863/1‑150 (MQ=255)
                                                                                                         cgcgACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATgg                                              <  1:1346881/150‑1 (MQ=255)
                                                                                                           cgACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATgggg                                            <  2:3329914/150‑1 (MQ=255)
                                                                                                             aCGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCt                                          >  1:1006482/1‑150 (MQ=255)
                                                                                                               gCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTAt                                        >  2:5196476/1‑150 (MQ=255)
                                                                                                                cAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATc                                       <  2:487092/150‑1 (MQ=255)
                                                                                                                 aaGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCg                                      >  2:1798299/1‑150 (MQ=255)
                                                                                                                    aTCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTa                                   <  1:2887370/150‑1 (MQ=255)
                                                                                                                     tctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTaa                                  >  1:5050166/1‑150 (MQ=255)
                                                                                                                      ctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAg                                 >  1:4429584/1‑150 (MQ=255)
                                                                                                                       tcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGc                                >  1:4933962/1‑150 (MQ=255)
                                                                                                                         cGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaa                              <  1:4771165/150‑1 (MQ=255)
                                                                                                                          gACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaa                             >  2:2052975/1‑150 (MQ=255)
                                                                                                                           aCAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaaa                            >  2:1312471/1‑150 (MQ=255)
                                                                                                                            cAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAt                           <  2:5453631/150‑1 (MQ=255)
                                                                                                                             agagCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtc                          >  1:2555490/1‑150 (MQ=255)
                                                                                                                               agCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAATCAAAAtctc                        >  1:2410352/1‑150 (MQ=255)
                                                                                                                               agCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtctc                        >  1:69276/1‑150 (MQ=255)
                                                                                                                                gCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCg                       <  2:369598/150‑1 (MQ=255)
                                                                                                                                gCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCg                       <  2:1047189/150‑1 (MQ=255)
                                                                                                                                 ccTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGc                      >  2:1921555/1‑150 (MQ=255)
                                                                                                                                       caCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGtt                <  2:5608922/150‑1 (MQ=255)
                                                                                                                                       caCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGtt                <  2:2392920/150‑1 (MQ=255)
                                                                                                                                        actactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTg               <  1:559000/150‑1 (MQ=255)
                                                                                                                                           actGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCgg            >  1:3091263/1‑150 (MQ=255)
                                                                                                                                            ctGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCggg           <  2:4224806/150‑1 (MQ=255)
                                                                                                                                            ctGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCggg           >  2:397139/1‑150 (MQ=255)
                                                                                                                                            ctGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCggg           <  1:954312/150‑1 (MQ=255)
                                                                                                                                              gCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTc         >  2:3923272/1‑150 (MQ=255)
                                                                                                                                               ccAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTcc        >  1:1999911/1‑150 (MQ=255)
                                                                                                                                                cAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTccc       >  1:1286755/1‑150 (MQ=255)
                                                                                                                                                  ggcgaggcgaGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCAt     <  2:5499710/150‑1 (MQ=255)
                                                                                                                                                    cgaggcgagTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATgc   >  2:5157764/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATGCG  >  CP009273/923190‑923488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A