Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F56 I0 R1 543 285.0 9366284 95.7% 8963533 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 923,339 G→T 25.6% S438* (TCG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273923,3390GT25.6% 635.5 / 203.2 270S438* (TCG→TAG) cydCfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (118/83);  new base T (33/36);  total (151/119)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 285 total aligned reads displayed.

ACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATG  >  CP009273/923191‑923486
                                                                                                                                                    |                                                                                                                                                   
  gggCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggcgag                                                                                                                                                   <  2:1465811/149‑1 (MQ=255)
    gCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggctaggc                                                                                                                                                 <  1:2074450/149‑1 (MQ=255)
      gATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAggctaggcga                                                                                                                                               <  1:1210376/149‑1 (MQ=255)
       aTAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGgctaggcgag                                                                                                                                              <  1:606555/149‑1 (MQ=255)
       aTAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGgcgaggcgag                                                                                                                                              <  2:3820462/149‑1 (MQ=255)
         aGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTa                                                                                                                                            >  2:107948/1‑149 (MQ=255)
         aGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTa                                                                                                                                            <  2:2077047/149‑1 (MQ=255)
            cAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTaaaa                                                                                                                                         <  1:3917727/149‑1 (MQ=255)
             aGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAg                                                                                                                                        >  2:4656064/1‑149 (MQ=255)
             aGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAg                                                                                                                                        >  2:1905671/1‑149 (MQ=255)
              gACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGa                                                                                                                                       <  1:3596104/149‑1 (MQ=255)
              gACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGa                                                                                                                                       <  1:2035645/149‑1 (MQ=255)
                cGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGAtt                                                                                                                                     <  2:188499/149‑1 (MQ=255)
                  gcgcAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTAt                                                                                                                                   <  1:2115747/149‑1 (MQ=255)
                  gcgcAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTAt                                                                                                                                   <  1:3421018/149‑1 (MQ=255)
                   cgcAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATc                                                                                                                                  <  2:280176/149‑1 (MQ=255)
                      aGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACg                                                                                                                               >  1:3181980/1‑149 (MQ=255)
                       gTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGc                                                                                                                              >  2:1812223/1‑149 (MQ=255)
                       gTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGAACAAGATTATCACGc                                                                                                                              >  2:4293714/1‑149 (MQ=255)
                        ttCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCa                                                                                                                             >  1:3098590/1‑149 (MQ=255)
                           accaccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCg                                                                                                                          <  2:4351211/149‑1 (MQ=255)
                             caccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTg                                                                                                                        <  1:3704203/149‑1 (MQ=255)
                                 ggAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTCCTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGgcgc                                                                                                                    >  2:3258090/1‑149 (MQ=255)
                                 ggAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGgcgc                                                                                                                    >  2:3211431/1‑149 (MQ=255)
                                 ggAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGgcgc                                                                                                                    >  2:2854743/1‑149 (MQ=255)
                                  gagaGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCt                                                                                                                   >  1:2892909/1‑149 (MQ=255)
                                  gagaGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCt                                                                                                                   <  1:952431/149‑1 (MQ=255)
                                     agCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTaaa                                                                                                                <  2:2368187/149‑1 (MQ=255)
                                         ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                            <  1:441059/149‑1 (MQ=255)
                                         ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                            >  1:1024800/1‑149 (MQ=255)
                                            gTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGc                                                                                                         >  1:2731595/1‑149 (MQ=255)
                                             tCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCa                                                                                                        >  1:577796/1‑149 (MQ=255)
                                                gCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTc                                                                                                     >  2:850505/1‑149 (MQ=255)
                                                gCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTc                                                                                                     >  1:684900/1‑149 (MQ=255)
                                                  cTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGc                                                                                                   <  1:2143993/149‑1 (MQ=255)
                                                     cACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGa                                                                                                >  1:1725643/1‑149 (MQ=255)
                                                     cACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGa                                                                                                >  1:4598402/1‑149 (MQ=255)
                                                       ccTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAgg                                                                                              <  1:700068/149‑1 (MQ=255)
                                                         tAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaa                                                                                            >  2:4553038/1‑149 (MQ=255)
                                                          aaCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaac                                                                                           >  1:2715221/1‑149 (MQ=255)
                                                           aCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGAAAAAGATTATCACGCAGCGAGGCGCTAAACAGATGCACTCGCTGAGGaaca                                                                                          >  2:4003997/1‑149 (MQ=255)
                                                            ccAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaa                                                                                         >  2:2906383/1‑149 (MQ=255)
                                                              aaCTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACg                                                                                       >  1:530579/1‑149 (MQ=255)
                                                               aCTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGc                                                                                      <  1:3762003/149‑1 (MQ=255)
                                                                   ttGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGAt                                                                                  >  2:260885/1‑149 (MQ=255)
                                                                   ttGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGAt                                                                                  <  1:2955074/149‑1 (MQ=255)
                                                                     gagaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATgg                                                                                >  2:3631008/1‑149 (MQ=255)
                                                                     gagaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATgg                                                                                <  2:3720420/149‑1 (MQ=255)
                                                                     gagaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATgg                                                                                <  2:4176202/149‑1 (MQ=255)
                                                                       gaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTc                                                                              <  1:4252817/149‑1 (MQ=255)
                                                                       gaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTc                                                                              <  2:2752322/149‑1 (MQ=255)
                                                                        aCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCt                                                                             >  1:2630041/1‑149 (MQ=255)
                                                                         ccTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTg                                                                            >  2:903731/1‑149 (MQ=255)
                                                                          cTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGt                                                                           <  2:2515251/149‑1 (MQ=255)
                                                                           tGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTc                                                                          >  2:3382977/1‑149 (MQ=255)
                                                                           tGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTc                                                                          >  2:3202006/1‑149 (MQ=255)
                                                                           tGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTc                                                                          >  2:3201884/1‑149 (MQ=255)
                                                                            gCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCg                                                                         <  1:2625413/149‑1 (MQ=255)
                                                                                 cTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTagag                                                                    >  1:1950035/1‑149 (MQ=255)
                                                                                     agcagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGc                                                                <  1:3468528/149‑1 (MQ=255)
                                                                                      gcagcTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCt                                                               >  2:4056091/1‑149 (MQ=255)
                                                                                      gcagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCt                                                               <  1:4277872/149‑1 (MQ=255)
                                                                                       cagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCtt                                                              <  1:1517370/149‑1 (MQ=255)
                                                                                         gcTTTTCCAGGCCAACGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttca                                                            >  2:4160485/1‑149 (MQ=255)
                                                                                            tttCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttcattc                                                         >  1:257823/1‑149 (MQ=255)
                                                                                              tCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAg                                                       <  2:1679428/149‑1 (MQ=255)
                                                                                                 aGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggct                                                    >  2:3455324/1‑149 (MQ=255)
                                                                                                 aGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggct                                                    <  1:108058/149‑1 (MQ=255)
                                                                                                  ggCCAACGCGACGCAGGATCTCCGACAGAGCCTCATCGCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctg                                                   <  2:1109327/149‑1 (MQ=255)
                                                                                                  ggCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTAAggctg                                                   >  1:138123/1‑149 (MQ=255)
                                                                                                     cAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctggct                                                >  2:2314712/1‑149 (MQ=255)
                                                                                                     cAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggctggct                                                >  2:4654638/1‑149 (MQ=255)
                                                                                                      aaCGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTa                                               <  1:3070530/149‑1 (MQ=255)
                                                                                                      aaCGCGACGCAAGATCTCCTACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTa                                               <  1:3861915/149‑1 (MQ=255)
                                                                                                      aaCGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTa                                               <  1:1322772/149‑1 (MQ=255)
                                                                                                      aaCGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTa                                               >  1:2333509/1‑149 (MQ=255)
                                                                                                           gACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATgggg                                          <  2:4431744/149‑1 (MQ=255)
                                                                                                            aCGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGc                                         <  2:3365901/149‑1 (MQ=255)
                                                                                                            aCGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGc                                         >  2:1310540/1‑149 (MQ=255)
                                                                                                              gCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTCTGGGGCTa                                       >  2:4340427/1‑149 (MQ=255)
                                                                                                              gCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTa                                       <  2:598266/149‑1 (MQ=255)
                                                                                                               cAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTAt                                      <  1:325974/149‑1 (MQ=255)
                                                                                                                  gATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGt                                   >  1:4366631/1‑149 (MQ=255)
                                                                                                                    tctcCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTa                                 >  2:2115396/1‑149 (MQ=255)
                                                                                                                    tctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTa                                 >  1:1506507/1‑149 (MQ=255)
                                                                                                                       ccGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGc                              >  1:4629770/1‑149 (MQ=255)
                                                                                                                       ccGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGGTAAGc                              >  1:4119036/1‑149 (MQ=255)
                                                                                                                         gACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaa                            >  2:1415124/1‑149 (MQ=255)
                                                                                                                           cAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaaa                          >  2:3566509/1‑149 (MQ=255)
                                                                                                                           cAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaaa                          <  2:791012/149‑1 (MQ=255)
                                                                                                                              agCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTAAGGCTGGCTATGGGGCTATCGTTAAGAAAAAtct                       >  1:901218/1‑149 (MQ=255)
                                                                                                                                 cTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGc                    <  1:3238287/149‑1 (MQ=255)
                                                                                                                                   catcaCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGccc                  >  2:3005037/1‑149 (MQ=255)
                                                                                                                                      caCTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGt               <  1:1572787/149‑1 (MQ=255)
                                                                                                                                       actactGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATATCGCCCAGtt              >  1:636584/1‑149 (MQ=255)
                                                                                                                                       actactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGtt              >  2:1952946/1‑149 (MQ=255)
                                                                                                                                        ctactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTg             >  2:734234/1‑149 (MQ=255)
                                                                                                                                         tactGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGc            <  1:1030962/149‑1 (MQ=255)
                                                                                                                                          actGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCg           <  1:816729/149‑1 (MQ=255)
                                                                                                                                           ctGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCgg          >  2:2264863/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATG  >  CP009273/923191‑923486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A