Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 923,339 G→T 14.4% S438* (TCG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273923,3390GT14.4% 686.9 / 71.7 216S438* (TCG→TAG) cydCfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (101/83);  new base T (26/6);  total (127/89)
Fisher's exact test for biased strand distribution p-value = 5.93e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

ACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATGCG  >  CP009273/923191‑923488
                                                                                                                                                    |                                                                                                                                                     
aCGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggcga                                                                                                                                                      >  2:1988177/1‑150 (MQ=255)
  gggCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggctagg                                                                                                                                                    >  2:4212137/1‑150 (MQ=255)
    gCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCaggctaggcg                                                                                                                                                  >  1:516091/1‑150 (MQ=255)
      gATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGgctaggcgag                                                                                                                                                >  1:1564135/1‑150 (MQ=255)
         aGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTaa                                                                                                                                             >  2:1263008/1‑150 (MQ=255)
         aGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTaa                                                                                                                                             <  1:1727904/150‑1 (MQ=255)
         aGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTaa                                                                                                                                             <  1:2321048/150‑1 (MQ=255)
           ccAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTaaaa                                                                                                                                           <  2:5077683/150‑1 (MQ=255)
            cAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAg                                                                                                                                          >  1:233095/1‑150 (MQ=255)
                 ggCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTAt                                                                                                                                     <  1:2437934/150‑1 (MQ=255)
                    gcAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCAc                                                                                                                                  <  1:3216709/150‑1 (MQ=255)
                      aGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGc                                                                                                                                >  1:4004573/1‑150 (MQ=255)
                      aGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGc                                                                                                                                <  1:1322034/150‑1 (MQ=255)
                        ttCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGATACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGATCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAg                                                                                                                              >  2:1788520/1‑150 (MQ=255)
                        ttCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAg                                                                                                                              >  2:2909126/1‑150 (MQ=255)
                          caccacCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCg                                                                                                                            <  1:4398064/150‑1 (MQ=255)
                          caccacCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCg                                                                                                                            >  1:4342310/1‑150 (MQ=255)
                           accaccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGt                                                                                                                           >  1:707766/1‑150 (MQ=255)
                           accaccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGt                                                                                                                           >  2:2367784/1‑150 (MQ=255)
                           accaccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGt                                                                                                                           >  1:3372355/1‑150 (MQ=255)
                            ccaccGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTg                                                                                                                          <  2:5172928/150‑1 (MQ=255)
                              accGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGgc                                                                                                                        >  2:1357389/1‑150 (MQ=255)
                                    gagCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTaaa                                                                                                                  >  1:3771708/1‑150 (MQ=255)
                                     agCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAAc                                                                                                                 >  1:4104066/1‑150 (MQ=255)
                                        tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              <  1:1025912/150‑1 (MQ=255)
                                        tGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGa                                                                                                              <  1:3624745/150‑1 (MQ=255)
                                         ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGAt                                                                                                             >  1:4617020/1‑150 (MQ=255)
                                         ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGAt                                                                                                             >  1:728303/1‑150 (MQ=255)
                                         ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGAt                                                                                                             >  2:4516957/1‑150 (MQ=255)
                                         ggCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGAt                                                                                                             <  1:3610217/150‑1 (MQ=255)
                                           cGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGc                                                                                                           <  1:1522695/150‑1 (MQ=255)
                                            gTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCa                                                                                                          >  1:1498708/1‑150 (MQ=255)
                                             tCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCAc                                                                                                         >  1:3169313/1‑150 (MQ=255)
                                               cGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTc                                                                                                       >  1:1471698/1‑150 (MQ=255)
                                                gCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCg                                                                                                      >  2:719334/1‑150 (MQ=255)
                                                  cTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCt                                                                                                    <  2:3500637/150‑1 (MQ=255)
                                                   ttCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTACAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTg                                                                                                   >  2:358434/1‑150 (MQ=255)
                                                   ttCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTg                                                                                                   >  1:3963105/1‑150 (MQ=255)
                                                          aaCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaaca                                                                                            >  1:1852716/1‑150 (MQ=255)
                                                           aCCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaa                                                                                           >  2:89450/1‑150 (MQ=255)
                                                            ccAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaac                                                                                          >  1:644392/1‑150 (MQ=255)
                                                            ccAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGaacaac                                                                                          >  1:3450566/1‑150 (MQ=255)
                                                             cAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACg                                                                                         >  1:3658019/1‑150 (MQ=255)
                                                                   ttGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATg                                                                                   <  1:4858910/150‑1 (MQ=255)
                                                                    tGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATgg                                                                                  >  2:4484502/1‑150 (MQ=255)
                                                                     gagaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGACCAACGCTGATGGt                                                                                 >  2:1161740/1‑150 (MQ=255)
                                                                       gaCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCt                                                                               >  2:2923431/1‑150 (MQ=255)
                                                                        aCCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTg                                                                              >  2:2137866/1‑150 (MQ=255)
                                                                          cTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTc                                                                            >  2:3414928/1‑150 (MQ=255)
                                                                           tGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCg                                                                           <  1:3071643/150‑1 (MQ=255)
                                                                                ccTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGCGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTagag                                                                      >  2:3910399/1‑150 (MQ=255)
                                                                                   cGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCg                                                                   <  1:4783117/150‑1 (MQ=255)
                                                                                     agcagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCt                                                                 <  1:2246808/150‑1 (MQ=255)
                                                                                       cagcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttc                                                               >  1:1208262/1‑150 (MQ=255)
                                                                                        agcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGACCAACGCTGATGGTCTGTCGTAGAGCCGCttaa                                                              >  1:1678413/1‑148 (MQ=255)
                                                                                        agcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttca                                                              >  2:269379/1‑150 (MQ=255)
                                                                                         gcTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttcat                                                             <  2:4199789/150‑1 (MQ=255)
                                                                                            tttCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCttcattca                                                          <  2:3808955/150‑1 (MQ=255)
                                                                                              tCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAgg                                                        >  1:3403765/1‑150 (MQ=255)
                                                                                              tCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAgg                                                        >  2:3945718/1‑150 (MQ=255)
                                                                                               ccAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggc                                                       >  2:3691789/1‑150 (MQ=255)
                                                                                               ccAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggc                                                       >  1:3910886/1‑150 (MQ=255)
                                                                                                cAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAggct                                                      <  2:225138/150‑1 (MQ=255)
                                                                                                     cAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTa                                                 >  2:641530/1‑150 (MQ=255)
                                                                                                      aaCGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTAt                                                >  1:2012035/1‑150 (MQ=255)
                                                                                                        cgcgACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATgg                                              <  1:913301/150‑1 (MQ=255)
                                                                                                          cgACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATgggg                                            <  1:1689532/150‑1 (MQ=255)
                                                                                                           gACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGc                                           >  1:1147732/1‑150 (MQ=255)
                                                                                                            aCGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCt                                          <  1:3261/150‑1 (MQ=255)
                                                                                                             cGCAATATCTCCGACAGAGCCTCATCACTAGTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTa                                         <  2:3170791/150‑1 (MQ=255)
                                                                                                             cGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCGGGCTATGGGGCTa                                         >  2:2423696/1‑150 (MQ=255)
                                                                                                               cAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATc                                       >  2:1001738/1‑150 (MQ=255)
                                                                                                               cAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATc                                       <  2:4255185/150‑1 (MQ=255)
                                                                                                                aaGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTCTCg                                      >  2:3285077/1‑150 (MQ=255)
                                                                                                                aaGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCg                                      >  1:2673133/1‑150 (MQ=255)
                                                                                                                  gATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGtt                                    >  2:3208029/1‑150 (MQ=255)
                                                                                                                    tctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTaa                                  >  2:4080908/1‑150 (MQ=255)
                                                                                                                     ctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAg                                 <  1:782867/150‑1 (MQ=255)
                                                                                                                     ctcCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAg                                 <  1:782714/150‑1 (MQ=255)
                                                                                                                         gACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaa                             >  2:729941/1‑150 (MQ=255)
                                                                                                                         gACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaa                             >  2:4616632/1‑150 (MQ=255)
                                                                                                                          aCAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCaaaa                            <  2:876063/150‑1 (MQ=255)
                                                                                                                           cAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGACCAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTCTGGGGCTATCGTTAAGCAAAAt                           >  2:1262212/1‑150 (MQ=255)
                                                                                                                           cAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAt                           >  2:4938197/1‑150 (MQ=255)
                                                                                                                            agagCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTTAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtc                          >  1:4092040/1‑150 (MQ=255)
                                                                                                                              agCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtctc                        <  1:2131210/150‑1 (MQ=255)
                                                                                                                              agCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtctc                        >  2:2236935/1‑150 (MQ=255)
                                                                                                                              agCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAAtctc                        <  2:3871984/150‑1 (MQ=255)
                                                                                                                                 cTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAAAAGATGCACTCGCTGAGGAACAACGCTGATGGTCTATCGTAGAGCCCCTACATACAGGCTGTCTATGGGGCTATCGTAAAGCAAAATCTAGcc                     >  2:1691312/1‑150 (MQ=255)
                                                                                                                                  tcatcaCTACTGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGGTATCGTTAAGCAAAATCTCGccc                    >  1:1365957/1‑150 (MQ=255)
                                                                                                                                   catcaCTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCt                   <  1:969102/150‑1 (MQ=255)
                                                                                                                                       actactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTg               <  1:4652164/150‑1 (MQ=255)
                                                                                                                                       actactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTg               <  2:1132741/150‑1 (MQ=255)
                                                                                                                                        ctactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGc              <  2:3389200/150‑1 (MQ=255)
                                                                                                                                         tactGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTAAGGCTGGCTATGGGGCTATCGTTAATCAAAATATCGCCCTGTTGCg             >  1:571216/1‑150 (MQ=255)
                                                                                                                                          actGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCGATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCgg            >  1:3475383/1‑150 (MQ=255)
                                                                                                                                           ctGCCAGGCTAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCggg           >  2:4774154/1‑150 (MQ=255)
                                                                                                                                               cAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTccc       <  2:4342310/150‑1 (MQ=255)
                                                                                                                                                 ggcgaggcgaGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCGGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCAt     <  2:194841/150‑1 (MQ=255)
                                                                                                                                                    gaggcgagTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATgcg  >  2:4874863/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGGGCGATAGCCAGACGGCGCAGTTCACCACCGGAGAGCTGGCGTCCGCCTTCACCTAACCAACTGTTGAGACCTGCATCCTCGAGCAGCTTTTCCAGGCCAACGCGACGCAAGATCTCCGACAGAGCCTCATCACTACTGCCAGGCGAGGCGAGTAAAAGATTATCACGCAGCGTGGCGCTAAACAGATGCACTCGCTGAGGAACAACGCTGATGGTCTGTCGTAGAGCCGCTTCATTCAGGCTGGCTATGGGGCTATCGTTAAGCAAAATCTCGCCCTGTTGCGGGTCCCATGCG  >  CP009273/923191‑923488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A