Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,151,750 G→T 46.3% R178L (CGC→CTC)  holB → DNA polymerase III, delta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,151,7500GT46.3% 151.0 / inf 287R178L (CGC→CTC) holBDNA polymerase III, delta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (81/72);  new base T (70/63);  total (152/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  CP009273/1151604‑1151898
                                                                                                                                                  |                                                                                                                                                    
gAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTATCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGa                                                                                                                                                   >  2:1652811/1‑150 (MQ=255)
gAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGa                                                                                                                                                   >  2:4260757/1‑150 (MQ=255)
   aaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGt                                                                                                                                                >  1:262706/1‑150 (MQ=255)
     cGCTTGAAGAGCCACCATCAGAAACTGTGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGa                                                                                                                                              <  2:3971019/150‑1 (MQ=255)
      gCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGAc                                                                                                                                             <  1:4096996/150‑1 (MQ=255)
         tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAt                                                                                                                                          >  2:3272571/1‑150 (MQ=255)
         tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCAGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCCTGACCTGGCTTTAACGCGAAGTGACAAt                                                                                                                                          >  1:4639038/1‑150 (MQ=255)
           aaGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGt                                                                                                                                        >  2:715452/1‑150 (MQ=255)
           aaGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGt                                                                                                                                        >  1:1673154/1‑150 (MQ=255)
              agCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTcac                                                                                                                                     >  1:578951/1‑150 (MQ=255)
              agCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTcac                                                                                                                                     >  1:2073935/1‑150 (MQ=255)
               gCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTcaca                                                                                                                                    <  1:2241228/150‑1 (MQ=255)
                ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAg                                                                                                                                   >  1:1095800/1‑150 (MQ=255)
                ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAg                                                                                                                                   >  2:3553180/1‑150 (MQ=255)
                ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAg                                                                                                                                   >  2:2021785/1‑150 (MQ=255)
                ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAg                                                                                                                                   <  1:1017311/150‑1 (MQ=255)
                   ccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGAt                                                                                                                                >  2:1290641/1‑150 (MQ=255)
                   ccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGAt                                                                                                                                >  1:913851/1‑150 (MQ=255)
                   ccaGCAGAAACTTGGTTTTTCCTGGCGACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGAt                                                                                                                                <  2:4961112/150‑1 (MQ=255)
                      gcagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCa                                                                                                                             >  2:4625937/1‑150 (MQ=255)
                       cagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGATTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCAt                                                                                                                            <  2:3854316/150‑1 (MQ=255)
                        agAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAtt                                                                                                                           <  2:2323476/150‑1 (MQ=255)
                           aaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                        <  2:4053759/150‑1 (MQ=255)
                             cTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTg                                                                                                                      >  1:1851908/1‑150 (MQ=255)
                             cTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTg                                                                                                                      <  1:4377001/150‑1 (MQ=255)
                               tGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGcc                                                                                                                    >  1:3739526/1‑150 (MQ=255)
                                 gTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTGACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTACACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGc                                                                                                                  >  2:1503248/1‑150 (MQ=255)
                                   ttttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAt                                                                                                                >  1:4412549/1‑150 (MQ=255)
                                    tttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAtt                                                                                                               <  1:498830/150‑1 (MQ=255)
                                    tttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAtt                                                                                                               <  1:2012635/150‑1 (MQ=255)
                                    tttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAtt                                                                                                               >  2:5090484/1‑150 (MQ=255)
                                    tttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAtt                                                                                                               <  1:3230918/150‑1 (MQ=255)
                                     ttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTg                                                                                                              >  2:4503714/1‑150 (MQ=255)
                                      tCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgc                                                                                                             <  2:3632897/150‑1 (MQ=255)
                                              aCCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAgc                                                                                                     <  2:4295344/150‑1 (MQ=255)
                                              aCCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAgc                                                                                                     >  1:2020323/1‑150 (MQ=255)
                                                     aGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                              <  2:1238859/150‑1 (MQ=255)
                                                     aGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                              <  1:4737225/150‑1 (MQ=255)
                                                      gCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTc                                                                                             <  2:2319688/150‑1 (MQ=255)
                                                       ccTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCg                                                                                            <  2:2619902/150‑1 (MQ=255)
                                                          gAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCt                                                                                         <  1:2383021/150‑1 (MQ=255)
                                                          gAACGTTGACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCt                                                                                         <  2:804427/150‑1 (MQ=255)
                                                              gTTTACTGGCAACCTTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGgcg                                                                                     <  2:474190/150‑1 (MQ=255)
                                                                ttACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGcgcg                                                                                   >  1:19616/1‑150 (MQ=255)
                                                                 tACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCgg                                                                                  >  1:4155984/1‑150 (MQ=255)
                                                                    tGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTCACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCAGTACATGCCGCAGTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                               >  2:2974062/1‑150 (MQ=255)
                                                                     ggCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACt                                                                              >  2:1647812/1‑150 (MQ=255)
                                                                     ggCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACt                                                                              <  1:4575217/150‑1 (MQ=255)
                                                                               cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGttt                                                                    >  1:3562449/1‑150 (MQ=255)
                                                                                gTAGTCGTTGTCGGTGACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTc                                                                   <  2:3097657/150‑1 (MQ=255)
                                                                                 tAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCa                                                                  >  2:3446754/1‑150 (MQ=255)
                                                                                  aGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAATCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAg                                                                 >  2:4140628/1‑150 (MQ=255)
                                                                                  aGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAg                                                                 <  1:1485101/150‑1 (MQ=255)
                                                                                     cGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGga                                                              >  1:3384685/1‑150 (MQ=255)
                                                                                      gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGgag                                                             >  2:496747/1‑150 (MQ=255)
                                                                                      gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGgag                                                             >  1:1815551/1‑150 (MQ=255)
                                                                                         gTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATa                                                          >  2:1043813/1‑150 (MQ=255)
                                                                                         gTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATa                                                          <  2:779572/150‑1 (MQ=255)
                                                                                          tCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATaa                                                         <  2:3899497/150‑1 (MQ=255)
                                                                                           cGGTTACATTACCTTGCGCCGCCGCCGGAACAGGACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAc                                                        <  1:3128275/150‑1 (MQ=255)
                                                                                            ggTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACt                                                       <  2:3364121/150‑1 (MQ=255)
                                                                                            ggTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACt                                                       >  2:2140650/1‑150 (MQ=255)
                                                                                             gTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                      <  2:4407378/150‑1 (MQ=255)
                                                                                             gTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                      >  2:1195540/1‑150 (MQ=255)
                                                                                                aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCa                                                   <  1:533938/150‑1 (MQ=255)
                                                                                                 cATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAg                                                  >  1:1285468/1‑150 (MQ=255)
                                                                                                   ttACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGc                                                >  1:2540476/1‑150 (MQ=255)
                                                                                                    tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                               >  2:2518947/1‑150 (MQ=255)
                                                                                                    tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                               >  2:256083/1‑150 (MQ=255)
                                                                                                     aCCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTc                                              <  1:1451331/150‑1 (MQ=255)
                                                                                                     aCCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTc                                              <  1:615364/150‑1 (MQ=255)
                                                                                                      ccTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCg                                             <  1:4795749/150‑1 (MQ=255)
                                                                                                              cgccgccgGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACAt                                     <  1:1715531/150‑1 (MQ=255)
                                                                                                               gccgccgGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGAGCGGCACTGGAGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACAtt                                    <  1:4612234/150‑1 (MQ=255)
                                                                                                                 cgccgGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATtgt                                  <  1:975057/150‑1 (MQ=255)
                                                                                                                       aaCAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAgg                            >  2:3780138/1‑150 (MQ=255)
                                                                                                                       aaCAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAgg                            <  1:3806311/150‑1 (MQ=255)
                                                                                                                       aaCAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAgg                            <  1:747891/150‑1 (MQ=255)
                                                                                                                       aaCAGTACGCCGTGACCAGGCTTTCACTAGAAGTGACAAAGTCACAGGATGCATTACTTGCCGCATTGCTCATAAGCGACTGTTCGCATGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAATGTCAGGCTCGAGAAACATtctggaagg                            >  2:4188920/1‑145 (MQ=255)
                                                                                                                        aCAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGc                           >  1:3664363/1‑150 (MQ=255)
                                                                                                                         cagttagCCGTGACCTGGCTTTCTCTCGATGTGAGAATGTGTCAGGATGCATTACTTGCCGCATTTCGCTTAAGCGACGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCg                          <  2:2776403/144‑1 (MQ=255)
                                                                                                                           gTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGtt                        >  1:2740657/1‑150 (MQ=255)
                                                                                                                               gCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACGTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCa                    <  2:4770423/150‑1 (MQ=255)
                                                                                                                                ccGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCat                   >  1:3497343/1‑150 (MQ=255)
                                                                                                                                  gTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTAGTGAAACATTGTGTCAGGCGTTGGCatat                 <  1:4686297/150‑1 (MQ=255)
                                                                                                                                   tGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCAtata                <  1:2891488/150‑1 (MQ=255)
                                                                                                                                       cTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGt            >  1:1174867/1‑150 (MQ=255)
                                                                                                                                       cTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGt            >  1:804786/1‑150 (MQ=255)
                                                                                                                                        tGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTg           >  2:1677612/1‑150 (MQ=255)
                                                                                                                                        tGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTg           >  1:2448536/1‑150 (MQ=255)
                                                                                                                                        tGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTg           <  2:4807086/150‑1 (MQ=255)
                                                                                                                                            tttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAt       >  1:2951600/1‑150 (MQ=255)
                                                                                                                                            tttCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAt       >  1:1794993/1‑150 (MQ=255)
                                                                                                                                            tttCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAt       >  2:857803/1‑150 (MQ=255)
                                                                                                                                             ttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATc      <  1:4875942/150‑1 (MQ=255)
                                                                                                                                              tCACTCGAAGTGACACTGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTAGTGTCAGGGAGATATCTGGCAGGCTCGTGTACCAGTGTGTCAGGCGGTGGCATAGAGCGTGCCATCg     >  2:3134854/1‑150 (MQ=255)
                                                                                                                                              tCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCg     >  2:1922538/1‑150 (MQ=255)
                                                                                                                                               cACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCng    <  1:2715103/150‑1 (MQ=255)
                                                                                                                                                 cgcgAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGc  >  2:1769893/1‑150 (MQ=255)
                                                                                                                                                 cgcgAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGAGTCAGGCGTTGGCATATAGCGTGCCATCGGGc  >  2:4376889/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  CP009273/1151604‑1151898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A