Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,151,750 G→T 100% R178L (CGC→CTC)  holB → DNA polymerase III, delta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,151,7500GT99.1% 767.4 / ‑2.5 247R178L (CGC→CTC) holBDNA polymerase III, delta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (114/129);  minor base A (2/2);  total (116/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 247 total aligned reads displayed.

AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT  >  CP009273/1151606‑1151893
                                                                                                                                                |                                                                                                                                               
   cGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGt                                                                                                                                           <  1:131121/148‑1 (MQ=255)
    gCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACCTTACCGTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTg                                                                                                                                          >  1:3013552/1‑148 (MQ=255)
       tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACa                                                                                                                                       >  1:2973186/1‑148 (MQ=255)
       tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACa                                                                                                                                       >  1:173487/1‑148 (MQ=255)
        gAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACaa                                                                                                                                      >  2:716993/1‑148 (MQ=255)
           gagCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGt                                                                                                                                   >  2:2969139/1‑148 (MQ=255)
               caccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTcaca                                                                                                                               <  2:1149222/148‑1 (MQ=255)
                 ccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAgg                                                                                                                             <  2:744075/148‑1 (MQ=255)
                  cagcagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGa                                                                                                                            <  1:1245520/148‑1 (MQ=255)
                  cagcagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGa                                                                                                                            <  1:2443900/148‑1 (MQ=255)
                    gcagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATg                                                                                                                          <  1:2296725/148‑1 (MQ=255)
                     cagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGc                                                                                                                         <  2:936923/148‑1 (MQ=255)
                     cagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGc                                                                                                                         <  2:936746/148‑1 (MQ=255)
                      agAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCa                                                                                                                        <  2:48058/148‑1 (MQ=255)
                        aaaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAtt                                                                                                                      >  1:1121016/1‑148 (MQ=255)
                        aaaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAtt                                                                                                                      >  2:2314989/1‑148 (MQ=255)
                         aaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTa                                                                                                                     <  1:684814/148‑1 (MQ=255)
                          acgtGGTTTTTCCTGGCGACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACAGTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATTCATTAc                                                                                                                    <  2:3138152/145‑1 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                    >  1:3218403/1‑148 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                    <  2:2456262/148‑1 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                    >  2:1142478/1‑148 (MQ=255)
                           cTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                   <  2:3391255/148‑1 (MQ=255)
                           cTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                   <  1:90625/148‑1 (MQ=255)
                           cTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATGACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                   <  2:2550555/148‑1 (MQ=255)
                             gggTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATGTCCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTg                                                                                                                 <  2:917822/147‑1 (MQ=255)
                              ggTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGc                                                                                                                <  2:554588/148‑1 (MQ=255)
                              ggTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGc                                                                                                                <  1:1426044/148‑1 (MQ=255)
                                tttttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCg                                                                                                              >  2:1597066/1‑148 (MQ=255)
                                   ttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAt                                                                                                           <  2:359161/148‑1 (MQ=255)
                                       tGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                       <  1:1544405/148‑1 (MQ=255)
                                       tGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                       <  2:1826361/148‑1 (MQ=255)
                                       tGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCACCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                       <  1:3186902/148‑1 (MQ=255)
                                        ggCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcgc                                                                                                      <  2:1280716/148‑1 (MQ=255)
                                         gCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCt                                                                                                     >  1:3143985/1‑148 (MQ=255)
                                          cTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTTTCGGTTAAATTAACGTGCGCCGCCCCCGGAACAGTACGCCCTGAACAGGGTTTCACACGAAGTGACAAAGTAACAGGATGCATTACTTGCCCCATTTCGCtt                                                                                                    >  1:798036/1‑148 (MQ=255)
                                            aCCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTaa                                                                                                  <  1:2093059/148‑1 (MQ=255)
                                             cccGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAg                                                                                                 <  2:499941/148‑1 (MQ=255)
                                               cgcgAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAgcg                                                                                               <  1:1044527/148‑1 (MQ=255)
                                                gcgAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAgcgc                                                                                              >  2:1852226/1‑148 (MQ=255)
                                                   aGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCgg                                                                                           >  2:2513850/1‑148 (MQ=255)
                                                     ccTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                         >  2:3451576/1‑148 (MQ=255)
                                                     ccTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                         >  2:2732736/1‑148 (MQ=255)
                                                      cTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTc                                                                                        >  2:2601496/1‑148 (MQ=255)
                                                       tGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTNCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCg                                                                                       >  2:1556815/1‑148 (MQ=255)
                                                       tGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCg                                                                                       >  2:179191/1‑148 (MQ=255)
                                                         aaCGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGcc                                                                                     <  1:1174895/148‑1 (MQ=255)
                                                         aaCGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGcc                                                                                     <  2:1097694/148‑1 (MQ=255)
                                                            gTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTgg                                                                                  >  2:287168/1‑148 (MQ=255)
                                                             tttACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGgc                                                                                 >  2:698282/1‑148 (MQ=255)
                                                             tttACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGgc                                                                                 >  2:697925/1‑148 (MQ=255)
                                                                   ggCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCa                                                                           <  2:2238771/148‑1 (MQ=255)
                                                                    gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                          >  2:1380900/1‑148 (MQ=255)
                                                                    gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                          >  1:2167434/1‑148 (MQ=255)
                                                                    gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                          >  1:270081/1‑148 (MQ=255)
                                                                           tACGTAGTCGTTGTCGGTTACATTAACTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCAATCGAAGTGACAAAGTCACAGGATTCAATACTTGCCGCATTTCGCTTAAGCGCCGGTTCGCCAGGCGCGGCACTGGCgtt                                                                   >  1:2516905/1‑148 (MQ=255)
                                                                            aCGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgttg                                                                  <  1:2804496/148‑1 (MQ=255)
                                                                             cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgttgt                                                                 <  1:3067064/148‑1 (MQ=255)
                                                                              gTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgttgtt                                                                <  1:2836339/148‑1 (MQ=255)
                                                                               tAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGttt                                                               <  2:1545853/148‑1 (MQ=255)
                                                                               tAGTCGTTGTCGGTTACATTACCTTGCGCCCCCGCCGGAACAGTACGCCGTGACCTTGCTTTAACTCGAAGTGACAAAGTAACAGGATGCATTAATTTCCCCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCCCTGGCGGTGttt                                                               >  1:48863/1‑148 (MQ=255)
                                                                                    gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAggg                                                          <  1:3349715/148‑1 (MQ=255)
                                                                                     ttGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGga                                                         >  2:2563689/1‑148 (MQ=255)
                                                                                     ttGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGga                                                         >  1:1662459/1‑148 (MQ=255)
                                                                                       gTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGgaga                                                       <  1:760536/148‑1 (MQ=255)
                                                                                        tCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGAt                                                      >  1:1079516/1‑148 (MQ=255)
                                                                                         cGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATa                                                     <  2:3279128/148‑1 (MQ=255)
                                                                                         cGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATa                                                     >  1:694130/1‑148 (MQ=255)
                                                                                            ttACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACt                                                  >  2:1995287/1‑148 (MQ=255)
                                                                                            ttACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACt                                                  <  1:796408/148‑1 (MQ=255)
                                                                                             tACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                 >  1:732870/1‑148 (MQ=255)
                                                                                             tACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                 >  1:408861/1‑148 (MQ=255)
                                                                                             tACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                 >  1:2176310/1‑148 (MQ=255)
                                                                                              aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTgg                                                >  1:653936/1‑148 (MQ=255)
                                                                                              aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTgg                                                >  2:838366/1‑148 (MQ=255)
                                                                                                      ttGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCg                                        >  2:1682559/1‑148 (MQ=255)
                                                                                                        gcgcCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTg                                      <  1:1688755/148‑1 (MQ=255)
                                                                                                          gccgccgccGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGaa                                    <  1:2907405/148‑1 (MQ=255)
                                                                                                              ccgccgGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGAATTGCGCTTAAGCGCCGTTTCGCCTGGCGCGTCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACAt                                <  2:334828/148‑1 (MQ=255)
                                                                                                                   ggAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTgtgt                           <  2:1487833/148‑1 (MQ=255)
                                                                                                                    gAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTc                          >  1:1488329/1‑148 (MQ=255)
                                                                                                                      aCAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAg                        >  1:2386562/1‑148 (MQ=255)
                                                                                                                      aCAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAg                        <  2:2365294/148‑1 (MQ=255)
                                                                                                                         gTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCg                     <  2:173487/148‑1 (MQ=255)
                                                                                                                          tACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGt                    >  2:1893867/1‑148 (MQ=255)
                                                                                                                          tACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGt                    >  2:221628/1‑148 (MQ=255)
                                                                                                                          tACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGt                    >  2:450621/1‑148 (MQ=255)
                                                                                                                               cGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTggaa               <  2:3231432/148‑3 (MQ=255)
                                                                                                                               cGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCa               <  2:1956473/148‑1 (MQ=255)
                                                                                                                               cGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCa               <  2:2441040/148‑1 (MQ=255)
                                                                                                                               cGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCa               <  1:2360954/148‑1 (MQ=255)
                                                                                                                                gTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCat              <  2:425416/148‑1 (MQ=255)
                                                                                                                                  gACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCatat            <  1:1324217/148‑1 (MQ=255)
                                                                                                                                   aCCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCAtata           >  2:942053/1‑148 (MQ=255)
                                                                                                                                    ccTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAg          <  1:2115662/148‑1 (MQ=255)
                                                                                                                                     cTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGc         >  2:2331164/1‑148 (MQ=255)
                                                                                                                                     cTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGc         <  2:816125/148‑1 (MQ=255)
                                                                                                                                       ggCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGt       >  1:2552027/1‑148 (MQ=255)
                                                                                                                                        gCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTg      >  2:674917/1‑148 (MQ=255)
                                                                                                                                         cTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGc     <  2:269429/148‑1 (MQ=255)
                                                                                                                                          tttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc    <  1:3489669/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT  >  CP009273/1151606‑1151893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  CP009273/1151601‑1151898
                                                                                                                                                     |                                                                                                                                                    
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACT                                                                                                                                                      <  SRR19973065.2894585/150‑1 (MQ=60)
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACT                                                                                                                                                      <  SRR19973065.1881214/150‑1 (MQ=60)
     AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAG                                                                                                                                                 <  SRR19973065.1606343/150‑1 (MQ=60)
      AACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGT                                                                                                                                                <  SRR19973065.328448/150‑1 (MQ=60)
       ACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACCTTACCGTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTG                                                                                                                                               >  SRR19973065.3032007/1‑150 (MQ=60)
       ACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTG                                                                                                                                               <  SRR19973065.399015/150‑1 (MQ=60)
       ACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTG                                                                                                                                               <  SRR19973065.399012/150‑1 (MQ=60)
        CGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGA                                                                                                                                              <  SRR19973065.132248/150‑1 (MQ=60)
          CTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACA                                                                                                                                            >  SRR19973065.2991369/1‑150 (MQ=60)
          CTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACA                                                                                                                                            >  SRR19973065.174870/1‑150 (MQ=60)
           TTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAA                                                                                                                                           >  SRR19973065.1679640/1‑150 (MQ=60)
            TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACACGAAGTGACAAT                                                                                                                                          <  SRR19973065.1260788/150‑1 (MQ=60)
            TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACACGAAGTGACAAT                                                                                                                                          <  SRR19973065.1261579/150‑1 (MQ=60)
                 AGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTCCACTCGAAGTGACAATGTCAC                                                                                                                                     >  SRR19973065.675822/1‑150 (MQ=60)
                      CCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGAT                                                                                                                                <  SRR19973065.317696/150‑1 (MQ=60)
                       CAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATG                                                                                                                               <  SRR19973065.1253718/150‑1 (MQ=60)
                       CAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATG                                                                                                                               <  SRR19973065.2459301/150‑1 (MQ=60)
                        AGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCAGGCTTTCACTCGAAGTGACAATGTCACAGGATGC                                                                                                                              >  SRR19973065.108031/1‑150 (MQ=60)
                         GCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCA                                                                                                                             <  SRR19973065.2311186/150‑1 (MQ=60)
                           AGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATT                                                                                                                           >  SRR19973065.1128322/1‑150 (MQ=60)
                           AGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATT                                                                                                                           >  SRR19973065.1128329/1‑150 (MQ=60)
                             AAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAC                                                                                                                         >  SRR19973065.1733677/1‑150 (MQ=60)
                             AAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAC                                                                                                                         >  SRR19973065.3237754/1‑150 (MQ=60)
                             AAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTAACTTGCGCCGCCGCCGGAACAATAAGCCGAGAACATGCTTTCACTAGAAGTGACAAATTCACACGATGCATTAC                                                                                                                         >  SRR19973065.1634625/1‑150 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                        <  SRR19973065.689621/150‑1 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                        <  SRR19973065.689442/150‑1 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                        <  SRR19973065.689365/150‑1 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAAGTCAAAGGATGCATTACT                                                                                                                        >  SRR19973065.1361878/1‑150 (MQ=60)
                                CTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTG                                                                                                                      <  SRR19973065.91521/150‑1 (MQ=60)
                                CTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTG                                                                                                                      <  SRR19973065.91485/150‑1 (MQ=60)
                                 TTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGC                                                                                                                     <  SRR19973065.3223519/150‑1 (MQ=60)
                                   GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                   >  SRR19973065.2210347/1‑150 (MQ=60)
                                   GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                   <  SRR19973065.1435335/150‑1 (MQ=60)
                                   GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                   <  SRR19973065.1434875/150‑1 (MQ=60)
                                   GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                   <  SRR19973065.1435334/150‑1 (MQ=60)
                                        TTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGTCAATGTCACAGGCAGCATTACTTtccgccgtg                                                                                                              >  SRR19973065.2372543/1‑141 (MQ=60)
                                        TTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGCTGCATTACTTGCCGCCTTG                                                                                                              >  SRR19973065.2372395/1‑150 (MQ=60)
                                        TTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCACATTG                                                                                                              <  SRR19973065.734271/150‑1 (MQ=60)
                                          CCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCG                                                                                                            >  SRR19973065.155417/1‑150 (MQ=60)
                                            TGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCT                                                                                                          >  SRR19973065.3163085/1‑150 (MQ=60)
                                            TGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCT                                                                                                          <  SRR19973065.1554618/150‑1 (MQ=60)
                                            TGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCACCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCT                                                                                                          <  SRR19973065.3206136/150‑1 (MQ=60)
                                             GGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTTTCGGTTAAATTAACGTGCGCCGCCCCCGGAACAGTACGCCCTGAACAGGGTTTCACACGAAGTGACAAAGTAACAGGATGCATTACTTGCCCCATTTCGCTT                                                                                                         >  SRR19973065.803310/1‑150 (MQ=60)
                                             GGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTT                                                                                                         >  SRR19973065.803156/1‑150 (MQ=60)
                                                 ACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGC                                                                                                     <  SRR19973065.2106505/150‑1 (MQ=60)
                                                 ACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGC                                                                                                     <  SRR19973065.2106481/150‑1 (MQ=60)
                                                 ACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGC                                                                                                     <  SRR19973065.3018746/150‑1 (MQ=60)
                                                    CGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCC                                                                                                  <  SRR19973065.1051431/150‑1 (MQ=60)
                                                            TGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCC                                                                                          >  SRR19973065.3128721/1‑150 (MQ=60)
                                                              AACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTG                                                                                        <  SRR19973065.1182370/150‑1 (MQ=60)
                                                              AACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTG                                                                                        <  SRR19973065.1182228/150‑1 (MQ=60)
                                                                       TGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAC                                                                               >  SRR19973065.2181453/1‑150 (MQ=60)
                                                                       TGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAC                                                                               >  SRR19973065.271907/1‑150 (MQ=60)
                                                                       TGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAC                                                                               >  SRR19973065.2181357/1‑150 (MQ=60)
                                                                        GGCAACATTACGTAGTCGTTGTCGGTTACATTAACATTCGCCCCCGCCCGAACAGTAAGCCGTGACCTGGCTTTGACTCGAAGTGACAAAGTCACAGGATTCATTACTTTCCGCATTGCGCGTAAGCGCCCGGTCGCCTGGCGCGGCACG                                                                              >  SRR19973065.1526272/1‑150 (MQ=60)
                                                                           AACATTACGTAGTCGTTGTCGGTTAAAATACCTTTCGCCGCCGCCGGAACAGTACGCCCTGACCAGGCTTTAACTCGAAGTGACAATTTCACAGGATGCATTACTTTCCGCCTTGCGCTTAAGCGCCCGTTCGCCTGGCGCGGCACTGGC                                                                           >  SRR19973065.1829741/1‑150 (MQ=60)
                                                                              ATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTT                                                                        >  SRR19973065.2532941/1‑150 (MQ=60)
                                                                              ATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTT                                                                        <  SRR19973065.2502048/150‑1 (MQ=60)
                                                                              ATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTT                                                                        <  SRR19973065.3276495/150‑1 (MQ=60)
                                                                              ATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACACGAAGTGACAAAGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACAGGCGTT                                                                        >  SRR19973065.2532955/1‑150 (MQ=60)
                                                                              ATTACGTAGTCGTTGTCGGTTACATTAACTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCAATCGAAGTGACAAAGTCACAGGATTCAATACTTGCCGCATTTCGCTTAAGCGCCGGTTCGCCAGGCGCGGCACTGGCGTT                                                                        >  SRR19973065.2532716/1‑150 (MQ=60)
                                                                                 ACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTT                                                                     <  SRR19973065.2821855/150‑1 (MQ=60)
                                                                                 ACGTAGTCGTTGTCGGTTACATGACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTT                                                                     <  SRR19973065.2109880/150‑1 (MQ=60)
                                                                                  CGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTT                                                                    <  SRR19973065.3085812/150‑1 (MQ=60)
                                                                                  CGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTT                                                                    <  SRR19973065.1329175/150‑1 (MQ=60)
                                                                                  CGTAGTCGTTGTCGGTTACATTACCTTGCGCCCCCGCCGGAACAGTACGCCGTGACCTTGCTTTAACTCGAAGTGACAAAGTAACAGGATGCATTAATTTCCCCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCCCTGGCGGTGTTT                                                                    >  SRR19973065.49419/1‑150 (MQ=60)
                                                                                   GTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTC                                                                   <  SRR19973065.2759368/150‑1 (MQ=60)
                                                                                   GTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTC                                                                   <  SRR19973065.2853285/150‑1 (MQ=60)
                                                                                   GTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTC                                                                   <  SRR19973065.2853803/150‑1 (MQ=60)
                                                                                   GGAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTC                                                                   <  SRR19973065.2853851/150‑1 (MQ=60)
                                                                                    TAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCA                                                                  <  SRR19973065.2129843/150‑1 (MQ=60)
                                                                                        CGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGA                                                              >  SRR19973065.1673595/1‑150 (MQ=60)
                                                                                         GTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAG                                                             <  SRR19973065.3370184/150‑1 (MQ=60)
                                                                                         GTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAG                                                             >  SRR19973065.2924144/1‑150 (MQ=60)
                                                                                           TGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGAT                                                           >  SRR19973065.1088139/1‑150 (MQ=60)
                                                                                           TGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGAT                                                           >  SRR19973065.1086644/1‑150 (MQ=60)
                                                                                            GTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATA                                                          >  SRR19973065.698754/1‑150 (MQ=60)
                                                                                            GTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATA                                                          <  SRR19973065.765189/150‑1 (MQ=60)
                                                                                            GTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATA                                                          <  SRR19973065.765604/150‑1 (MQ=60)
                                                                                              CGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAC                                                        <  SRR19973065.3472620/150‑1 (MQ=60)
                                                                                                GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                      >  SRR19973065.737773/1‑150 (MQ=60)
                                                                                                GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                      >  SRR19973065.2190269/1‑150 (MQ=60)
                                                                                                GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                      >  SRR19973065.411722/1‑150 (MQ=60)
                                                                                                GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                      >  SRR19973065.411858/1‑150 (MQ=60)
                                                                                                GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                      >  SRR19973065.739138/1‑150 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                     <  SRR19973065.801413/150‑1 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                     <  SRR19973065.594318/150‑1 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                     >  SRR19973065.658247/1‑150 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                     <  SRR19973065.801776/150‑1 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                     <  SRR19973065.801675/150‑1 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                     >  SRR19973065.2431368/1‑150 (MQ=60)
                                                                                                  TACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGC                                                    <  SRR19973065.1195980/150‑1 (MQ=60)
                                                                                                    CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                  >  SRR19973065.849227/1‑150 (MQ=60)
                                                                                                    CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                  >  SRR19973065.572343/1‑150 (MQ=60)
                                                                                                       tacctggggcggccgggGGAACAGTACGCCGTGACGTGCCTTTCACTCGAAGTGACATTTTCACAGGATGAATTACTTGCCGGATTGCGCTTAAGAGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCT                                               <  SRR19973065.2962548/133‑1 (MQ=60)
                                                                                                        ACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTC                                              <  SRR19973065.2049699/150‑1 (MQ=60)
                                                                                                        ACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTC                                              <  SRR19973065.3028215/150‑1 (MQ=60)
                                                                                                            TGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGA                                          >  SRR19973065.2721675/1‑150 (MQ=60)
                                                                                                             GCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAA                                         <  SRR19973065.1700032/150‑1 (MQ=60)
                                                                                                              CGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAA                                        <  SRR19973065.1676662/150‑1 (MQ=60)
                                                                                                               GCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAAC                                       <  SRR19973065.2925116/150‑1 (MQ=60)
                                                                                                                   CCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTG                                   <  SRR19973065.3234530/150‑1 (MQ=60)
                                                                                                                       CGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTC                               >  SRR19973065.1497855/1‑150 (MQ=60)
                                                                                                                        GGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCA                              >  SRR19973065.3058584/1‑150 (MQ=60)
                                                                                                                         GAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAG                             >  SRR19973065.2401537/1‑150 (MQ=60)
                                                                                                                         GAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAG                             >  SRR19973065.2401532/1‑150 (MQ=60)
                                                                                                                           ACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGC                           <  SRR19973065.2635507/150‑1 (MQ=60)
                                                                                                                                 CGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGC                     >  SRR19973065.2166216/1‑150 (MQ=60)
                                                                                                                                    CGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATA                  <  SRR19973065.2375638/150‑1 (MQ=60)
                                                                                                                                    CGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATA                  <  SRR19973065.2375669/150‑1 (MQ=60)
                                                                                                                                       GACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAG               <  SRR19973065.1332973/150‑1 (MQ=60)
                                                                                                                                       GACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAG               <  SRR19973065.3090599/150‑1 (MQ=60)
                                                                                                                                         CCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCG             <  SRR19973065.2129277/150‑1 (MQ=60)
                                                                                                                                          CTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGT            >  SRR19973065.2567999/1‑150 (MQ=60)
                                                                                                                                               TTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT       <  SRR19973065.3510891/150‑1 (MQ=60)
                                                                                                                                                 TCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCG     >  SRR19973065.3228606/1‑150 (MQ=60)
                                                                                                                                                   ACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGG   >  SRR19973065.850443/1‑150 (MQ=60)
                                                                                                                                                    CTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATATCGTGCCATCGGGC  >  SRR19973065.170288/1‑150 (MQ=60)
                                                                                                                                                    CTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  SRR19973065.340373/1‑150 (MQ=60)
                                                                                                                                                    CTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  SRR19973065.339814/1‑150 (MQ=60)
                                                                                                                                                    CTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  SRR19973065.170087/1‑150 (MQ=60)
                                                                                                                                                    CTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  SRR19973065.1045606/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC  >  CP009273/1151601‑1151898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑