Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,151,750 G→T 100% R178L (CGC→CTC)  holB → DNA polymerase III, delta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,151,7500GT100.0% 699.5 / ‑6.7 220R178L (CGC→CTC) holBDNA polymerase III, delta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (101/118);  minor base A (1/0);  total (102/118)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT  >  CP009273/1151606‑1151893
                                                                                                                                                |                                                                                                                                               
aaaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGa                                                                                                                                              >  2:2170130/1‑148 (MQ=255)
aaaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGa                                                                                                                                              <  2:1238218/148‑1 (MQ=255)
aaaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGa                                                                                                                                              >  1:2855918/1‑148 (MQ=255)
  aCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAg                                                                                                                                            <  2:220721/148‑1 (MQ=255)
  aCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAg                                                                                                                                            >  2:156026/1‑148 (MQ=255)
    gCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTg                                                                                                                                          <  2:1483762/148‑1 (MQ=255)
       tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACTTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACa                                                                                                                                       <  1:1749241/148‑1 (MQ=255)
       tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACa                                                                                                                                       >  2:3182322/1‑148 (MQ=255)
       tGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACa                                                                                                                                       <  1:1581997/148‑1 (MQ=255)
          agagCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATg                                                                                                                                    >  2:1263079/1‑148 (MQ=255)
             gCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTca                                                                                                                                 <  1:1030926/148‑1 (MQ=255)
             gCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTca                                                                                                                                 <  2:3313334/148‑1 (MQ=255)
             gCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGGca                                                                                                                                 >  2:3405308/1‑148 (MQ=255)
              ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTcac                                                                                                                                >  2:2288371/1‑148 (MQ=255)
                 ccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAgg                                                                                                                             <  1:1966378/148‑1 (MQ=255)
                 ccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAgg                                                                                                                             <  2:2754526/148‑1 (MQ=255)
                  cagcagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGa                                                                                                                            <  1:1885743/148‑1 (MQ=255)
                  cagcagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGa                                                                                                                            <  1:3099248/148‑1 (MQ=255)
                     cagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACGGGATGc                                                                                                                         <  2:3152843/148‑1 (MQ=255)
                     cagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGc                                                                                                                         >  1:2079175/1‑148 (MQ=255)
                     cagAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGc                                                                                                                         <  1:100929/148‑1 (MQ=255)
                       gAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAt                                                                                                                       <  1:2577514/148‑1 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                    >  2:853266/1‑148 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                    <  2:1978758/148‑1 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                    >  2:3539543/1‑148 (MQ=255)
                          aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACAATACCTTGCGCCGCCGCCGGAACAGTACGCCGTTACCTGGCTTTCACTCGAAGTGACAATCTCACAGCATGCATTAc                                                                                                                    >  2:3539527/1‑148 (MQ=255)
                            ttGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACtt                                                                                                                  <  1:243300/148‑1 (MQ=255)
                             tGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTg                                                                                                                 <  2:2676255/148‑1 (MQ=255)
                                tttttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCg                                                                                                              >  1:254649/1‑148 (MQ=255)
                                  tttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCa                                                                                                            <  1:991587/148‑1 (MQ=255)
                                       tGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                       <  1:605733/148‑1 (MQ=255)
                                         gCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCt                                                                                                     <  2:2387838/148‑1 (MQ=255)
                                         gCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCt                                                                                                     <  1:431795/148‑1 (MQ=255)
                                           tACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTa                                                                                                   >  2:838786/1‑148 (MQ=255)
                                             cccGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAg                                                                                                 <  1:417630/148‑1 (MQ=255)
                                                gcgAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAgcgc                                                                                              <  2:1120899/148‑1 (MQ=255)
                                                gcgAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAgcgc                                                                                              <  2:2224172/148‑1 (MQ=255)
                                                    gCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGt                                                                                          <  2:1194283/148‑1 (MQ=255)
                                                    gCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGt                                                                                          <  2:3215106/148‑1 (MQ=255)
                                                     ccTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                         >  2:2109953/1‑148 (MQ=255)
                                                     ccTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                         <  1:3061191/148‑1 (MQ=255)
                                                             tttACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGgc                                                                                 >  2:1823507/1‑148 (MQ=255)
                                                               tACTGGCAACATTACGTAGTCGTTGTCGGGTACAATACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTAACAGGATGCCTTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCAGgcgc                                                                               >  1:7137/1‑148 (MQ=255)
                                                                  tGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGc                                                                            <  1:212730/148‑1 (MQ=255)
                                                                    gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                          <  2:1664045/148‑1 (MQ=255)
                                                                    gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                          >  1:2360957/1‑148 (MQ=255)
                                                                    gCAACATTACGTAGTCGTGGTCGGTTACAATACCGTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTAAATCGAAGTGACAAAGTCACAGGATTCATTAATTTCCGCATTGCGCGTAAGCGCCGGTTCGCCTGGCGCGGCAc                                                                          >  1:3304374/1‑148 (MQ=255)
                                                                     cAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACt                                                                         >  2:1347018/1‑148 (MQ=255)
                                                                     cAACATTACGTAGTCGTTGTCGGTTACATTAACTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAGTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCGGGCGCGGCCCt                                                                         >  1:1035860/1‑148 (MQ=255)
                                                                      aaCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGTAGTGACATTGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTg                                                                        <  1:3246179/148‑1 (MQ=255)
                                                                      aaCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATTCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTg                                                                        <  2:1626509/148‑1 (MQ=255)
                                                                      aaCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTg                                                                        <  1:848531/148‑1 (MQ=255)
                                                                       aCATTACGTAGTCGTTGTCGGTTAAATTACCTTGCGCCGCCGCCGGAACCGTAAGCCCTGACCAGGCTTTCACTCGAAGTGACAAATTCACAGGATGCATTACTTGCCGCATTTCGCTTAAGCGCCGGTTCGCCAGGCGCGGCCCTgg                                                                       >  1:2460809/1‑148 (MQ=255)
                                                                        cATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGc                                                                      >  2:1367934/1‑148 (MQ=255)
                                                                         aTTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCg                                                                     <  2:2958255/148‑1 (MQ=255)
                                                                          ttACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgt                                                                    >  2:383658/1‑148 (MQ=255)
                                                                          ttACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgt                                                                    <  1:3539530/148‑1 (MQ=255)
                                                                           tACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgtt                                                                   <  1:2747159/148‑1 (MQ=255)
                                                                             cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgttgt                                                                 <  1:2949638/148‑1 (MQ=255)
                                                                             cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgttgt                                                                 <  2:702504/148‑1 (MQ=255)
                                                                                aGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTc                                                              <  2:732531/148‑1 (MQ=255)
                                                                                  tCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAg                                                            <  1:1361296/148‑1 (MQ=255)
                                                                                  tCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAg                                                            <  1:3439475/148‑1 (MQ=255)
                                                                                   cGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAgg                                                           >  2:1383945/1‑148 (MQ=255)
                                                                                          ggTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATaa                                                    <  2:2213428/148‑1 (MQ=255)
                                                                                           gTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAc                                                   <  1:838772/148‑1 (MQ=255)
                                                                                           gTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAc                                                   <  1:1515202/148‑1 (MQ=255)
                                                                                           gTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAc                                                   >  2:2565984/1‑148 (MQ=255)
                                                                                             tACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                 <  2:1511325/148‑1 (MQ=255)
                                                                                               cATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGc                                               >  1:1192057/1‑148 (MQ=255)
                                                                                                 ttACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAg                                             >  2:195215/1‑148 (MQ=255)
                                                                                                 ttACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAg                                             >  1:1641583/1‑148 (MQ=255)
                                                                                                 ttACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAg                                             >  2:211164/1‑148 (MQ=255)
                                                                                                  tACCTTGCGCCGCTGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAgg                                            <  2:1721390/148‑1 (MQ=255)
                                                                                                  tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAgg                                            <  1:68390/148‑1 (MQ=255)
                                                                                                  tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAgg                                            <  1:1873608/148‑1 (MQ=255)
                                                                                                   aCCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGc                                           >  2:764412/1‑148 (MQ=255)
                                                                                                    ccTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                          >  2:2325026/1‑148 (MQ=255)
                                                                                                    ccTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                          >  2:3522231/1‑148 (MQ=255)
                                                                                                     cTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTc                                         <  2:2904511/148‑1 (MQ=255)
                                                                                                         cgccgccgcCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGgaga                                                       <  2:1529205/130‑1 (MQ=255)
                                                                                                                      aCAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATACCTGGCAGGCTCGTGAAACCTTGTGTCAg                        >  2:457420/1‑148 (MQ=255)
                                                                                                                      aCAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAg                        >  2:168637/1‑148 (MQ=255)
                                                                                                                         gTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCg                     <  2:2229557/148‑1 (MQ=255)
                                                                                                                           aCGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGtt                   <  2:2957344/148‑1 (MQ=255)
                                                                                                                            cGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTg                  >  2:263562/1‑148 (MQ=255)
                                                                                                                              ccGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGc                >  2:2690632/1‑148 (MQ=255)
                                                                                                                              ccGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGGTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGc                >  2:2689566/1‑148 (MQ=255)
                                                                                                                               cGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCa               >  2:1021540/1‑148 (MQ=255)
                                                                                                                                gTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCat              >  2:3097193/1‑148 (MQ=255)
                                                                                                                                 tGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCata             <  2:3067247/148‑1 (MQ=255)
                                                                                                                                  gACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCatat            <  2:2605515/148‑1 (MQ=255)
                                                                                                                                  gACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACGTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCatat            <  1:3146733/148‑1 (MQ=255)
                                                                                                                                      tGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGATTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCg        >  2:2103310/1‑148 (MQ=255)
                                                                                                                                        gCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTg      >  1:1432778/1‑148 (MQ=255)
                                                                                                                                          tttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc    <  1:1951952/148‑1 (MQ=255)
                                                                                                                                          tttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc    >  1:105797/1‑148 (MQ=255)
                                                                                                                                           ttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCa   >  1:796813/1‑148 (MQ=255)
                                                                                                                                            tCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAt  <  2:1876039/148‑1 (MQ=255)
                                                                                                                                            tCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAt  >  1:2606228/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT  >  CP009273/1151606‑1151893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGG  >  CP009273/1151602‑1151896
                                                                                                                                                    |                                                                                                                                                  
CTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTC                                                                                                                                                   >  SRR19973066.2683249/1‑150 (MQ=60)
 TGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCG                                                                                                                                                  <  SRR19973066.2478748/150‑1 (MQ=60)
  GAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGA                                                                                                                                                 >  SRR19973066.2875101/1‑150 (MQ=60)
    AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAG                                                                                                                                               <  SRR19973066.1662984/150‑1 (MQ=60)
       CGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGA                                                                                                                                            <  SRR19973066.1229712/150‑1 (MQ=60)
           TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACTTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAT                                                                                                                                        <  SRR19973066.1761825/150‑1 (MQ=60)
           TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAT                                                                                                                                        >  SRR19973066.2262575/1‑150 (MQ=60)
           TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAT                                                                                                                                        <  SRR19973066.1899138/150‑1 (MQ=60)
           TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAT                                                                                                                                        <  SRR19973066.1593426/150‑1 (MQ=60)
           TGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAAT                                                                                                                                        >  SRR19973066.3082999/1‑150 (MQ=60)
            GAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATG                                                                                                                                       <  SRR19973066.464185/150‑1 (MQ=60)
             AAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGT                                                                                                                                      >  SRR19973066.668106/1‑150 (MQ=60)
             AAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGT                                                                                                                                      >  SRR19973066.2923911/1‑150 (MQ=60)
             AAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACAATACCATTCGCCCCCCCCGGCACAGTACCCCCAGCCCTGGCTTTAACTCGAAGTGACCATGT                                                                                                                                      >  SRR19973066.1598706/1‑150 (MQ=60)
                 GCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACA                                                                                                                                  <  SRR19973066.1038515/150‑1 (MQ=60)
                     CCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGAT                                                                                                                              <  SRR19973066.1980270/150‑1 (MQ=60)
                     CCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGAT                                                                                                                              <  SRR19973066.2906017/150‑1 (MQ=60)
                      CAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATG                                                                                                                             <  SRR19973066.3119947/150‑1 (MQ=60)
                      CAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATG                                                                                                                             <  SRR19973066.1899189/150‑1 (MQ=60)
                       agcagaggggggggggggggggggggggggggggggggggggtttaatgtaAACATTACGTATGCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGC                                                                                                                            >  SRR19973066.3521074/52‑150 (MQ=60)
                       AGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGC                                                                                                                            >  SRR19973066.2093536/1‑150 (MQ=60)
                       AGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGC                                                                                                                            >  SRR19973066.281431/1‑150 (MQ=60)
                         CAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAT                                                                                                                          <  SRR19973066.102004/150‑1 (MQ=60)
                         CAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAT                                                                                                                          <  SRR19973066.722337/150‑1 (MQ=60)
                           GAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTA                                                                                                                        <  SRR19973066.2595025/150‑1 (MQ=60)
                           GAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTA                                                                                                                        <  SRR19973066.2594997/150‑1 (MQ=60)
                                TTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGC                                                                                                                   <  SRR19973066.245201/150‑1 (MQ=60)
                                TTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGC                                                                                                                   <  SRR19973066.245199/150‑1 (MQ=60)
                                TTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGC                                                                                                                   <  SRR19973066.3022995/150‑1 (MQ=60)
                                  GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                 >  SRR19973066.256584/1‑150 (MQ=60)
                                  GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                 >  SRR19973066.256534/1‑150 (MQ=60)
                                      TTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATT                                                                                                             <  SRR19973066.998895/150‑1 (MQ=60)
                                      TTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATT                                                                                                             <  SRR19973066.998920/150‑1 (MQ=60)
                                           TGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCT                                                                                                        <  SRR19973066.526357/150‑1 (MQ=60)
                                           TGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCT                                                                                                        <  SRR19973066.609931/150‑1 (MQ=60)
                                             GCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTA                                                                                                      <  SRR19973066.435141/150‑1 (MQ=60)
                                                ACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGC                                                                                                   >  SRR19973066.1855004/1‑150 (MQ=60)
                                                 CCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCG                                                                                                  <  SRR19973066.420889/150‑1 (MQ=60)
                                                   CGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCC                                                                                                <  SRR19973066.852807/150‑1 (MQ=60)
                                                         CCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCG                                                                                          <  SRR19973066.3081668/150‑1 (MQ=60)
                                                           TGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCC                                                                                        >  SRR19973066.2566657/1‑150 (MQ=60)
                                                            GAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACCCCGTGACCAGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAATCGCCGGTTCGCCA                                                                                       >  SRR19973066.1033918/1‑150 (MQ=60)
                                                                 TTTACTGGCAACATTACGTAGTCGTTGTCGGGTACAATACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTAACAGGATGCCTTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCAGGCGC                                                                                  >  SRR19973066.7255/1‑150 (MQ=60)
                                                                     CTGGCAACATTACGTAGTCGTTGTCGGTTACAATAACTTGCGCCGCCGCCGGAACAGTACGCCGTGACCGGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCGTAAGCGCCGGTTCGCCTGGCGCGGCA                                                                              >  SRR19973066.975184/1‑150 (MQ=60)
                                                                      TGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAC                                                                             <  SRR19973066.214489/150‑1 (MQ=60)
                                                                      TGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCAC                                                                             >  SRR19973066.2376961/1‑150 (MQ=60)
                                                                      TGGCAACATTACGTAGTCGTGGTCGGTTACAATACCGTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTAAATCGAAGTGACAAAGTCACAGGATTCATTAATTTCCGCATTGCGCGTAAGCGCCGGTTCGCCTGGCGCGGCAC                                                                             >  SRR19973066.3326238/1‑150 (MQ=60)
                                                                       GGCAACATTACGTAGTCGTTGTCGGTTACATTACCATGCGCCGCCGCCGGAACAGTACGCCGTGACCGGGCTTTAACACGAAGTGACAATGTCACAGGATTCATTACTTGCCGCATTGCGCTTAAGCCCCGGTTCGCCTGGCGCGGCACT                                                                            >  SRR19973066.1043466/1‑150 (MQ=60)
                                                                       GGCAACATTACGTAGTCGTTGTCGGTTACATTAACTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCAGTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCGGGCGCGGCCCT                                                                            >  SRR19973066.1043481/1‑150 (MQ=60)
                                                                         CAACATTACGTAGTCGTTGTCGGTTAAATTACCTTGCGCCGCCGCCGGAACCGTAAGCCCTGACCAGGCTTTCACTCGAAGTGACAAATTCACAGGATGCATTACTTGCCGCATTTCGCTTAAGCGCCGGTTCGCCAGGCGCGGCCCTGG                                                                          >  SRR19973066.2477581/1‑150 (MQ=60)
                                                                          AACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGTAGTGACATTGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGC                                                                         <  SRR19973066.3267579/150‑1 (MQ=60)
                                                                          AACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGC                                                                         <  SRR19973066.3267801/150‑1 (MQ=60)
                                                                          AACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGC                                                                         <  SRR19973066.854677/150‑1 (MQ=60)
                                                                          AACATTACGTAGTCGTTGTCGGTTACAATAACTTTCGCCGCCGCCCGAACAGTAAGCCGTGACCAGGCGTTCACGCGAAGTGACAATTTCACAGGATTCATTAATTGCCGCCTTGCGCTTAAGCGCCGGGTCGCCTGGCGCGGCCCTGGC                                                                         >  SRR19973066.621935/1‑150 (MQ=60)
                                                                             ATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTT                                                                      <  SRR19973066.2678233/150‑1 (MQ=60)
                                                                              TTACGTCGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGGTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTG                                                                     <  SRR19973066.3563047/150‑1 (MQ=60)
                                                                              TTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTG                                                                     <  SRR19973066.3563063/150‑1 (MQ=60)
                                                                              TTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTG                                                                     <  SRR19973066.3563050/150‑1 (MQ=60)
                                                                               TACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGT                                                                    <  SRR19973066.2765639/150‑1 (MQ=60)
                                                                                ACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTT                                                                   <  SRR19973066.203973/150‑1 (MQ=60)
                                                                                ACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTT                                                                   <  SRR19973066.203972/150‑1 (MQ=60)
                                                                                 CGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTT                                                                  <  SRR19973066.2416557/150‑1 (MQ=60)
                                                                                 CGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTT                                                                  <  SRR19973066.2969211/150‑1 (MQ=60)
                                                                                      TCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGG                                                             <  SRR19973066.3462485/150‑1 (MQ=60)
                                                                                      TCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGG                                                             <  SRR19973066.1371143/150‑1 (MQ=60)
                                                                                            TCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAA                                                       <  SRR19973066.1524273/150‑1 (MQ=60)
                                                                                              GGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACT                                                     >  SRR19973066.2137313/1‑150 (MQ=60)
                                                                                               GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                    <  SRR19973066.1525842/150‑1 (MQ=60)
                                                                                               GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                    <  SRR19973066.844894/150‑1 (MQ=60)
                                                                                               GTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTG                                                    <  SRR19973066.844880/150‑1 (MQ=60)
                                                                                                TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                   >  SRR19973066.1091087/1‑150 (MQ=60)
                                                                                                 TACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGC                                                  >  SRR19973066.190352/1‑150 (MQ=60)
                                                                                                 TACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGC                                                  >  SRR19973066.1200400/1‑150 (MQ=60)
                                                                                                 TACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGC                                                  >  SRR19973066.1200463/1‑150 (MQ=60)
                                                                                                   CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                >  SRR19973066.1653524/1‑150 (MQ=60)
                                                                                                    ATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGG                                               <  SRR19973066.3320565/150‑1 (MQ=60)
                                                                                                      TACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCT                                             <  SRR19973066.1886984/150‑1 (MQ=60)
                                                                                                      TACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCT                                             <  SRR19973066.69214/150‑1 (MQ=60)
                                                                                                       ACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTC                                            >  SRR19973066.3192749/1‑150 (MQ=60)
                                                                                                           TGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAAgatcggaagagcaca                                        >  SRR19973066.1540061/1‑135 (MQ=60)
                                                                                                               CCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACA                                    <  SRR19973066.1377830/150‑1 (MQ=60)
                                                                                                               CCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACA                                    <  SRR19973066.3163324/150‑1 (MQ=60)
                                                                                                                CGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACAT                                   <  SRR19973066.425699/150‑1 (MQ=60)
                                                                                                                  CCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTG                                 >  SRR19973066.3327111/1‑150 (MQ=60)
                                                                                                                      CGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTCCTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTC                             <  SRR19973066.2502249/150‑1 (MQ=60)
                                                                                                                      CGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTCCTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTC                             <  SRR19973066.701445/150‑1 (MQ=60)
                                                                                                                                      GACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACGTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAG             <  SRR19973066.3167718/150‑1 (MQ=60)
                                                                                                                                        CCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCG           >  SRR19973066.1751897/1‑150 (MQ=60)
                                                                                                                                          TGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTG         >  SRR19973066.1442999/1‑150 (MQ=60)
                                                                                                                                            GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC       >  SRR19973066.106898/1‑150 (MQ=60)
                                                                                                                                            GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC       >  SRR19973066.105971/1‑150 (MQ=60)
                                                                                                                                             CTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCA      >  SRR19973066.802662/1‑150 (MQ=60)
                                                                                                                                             CTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCA      >  SRR19973066.802905/1‑150 (MQ=60)
                                                                                                                                              TTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT     >  SRR19973066.2623853/1‑150 (MQ=60)
                                                                                                                                              TTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT     <  SRR19973066.1965768/150‑1 (MQ=60)
                                                                                                                                              TTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCAT     <  SRR19973066.1965790/150‑1 (MQ=60)
                                                                                                                                                TCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGAGAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCG   >  SRR19973066.2056201/1‑150 (MQ=60)
                                                                                                                                                 CACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCTCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGG  >  SRR19973066.1273423/1‑150 (MQ=60)
                                                                                                                                                 CACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGG  >  SRR19973066.2332333/1‑150 (MQ=60)
                                                                                                                                                 CACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGAGCCATCGG  >  SRR19973066.1482816/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGG  >  CP009273/1151602‑1151896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑