Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,151,750 G→T R178L (CGC→CTC)  holB → DNA polymerase III, delta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,151,7500GT99.5% 706.8 / NA 211R178L (CGC→CTC) holBDNA polymerase III, delta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (108/102);  minor base A (1/0);  total (109/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

TGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCG  >  CP009273/1151603‑1151895
                                                                                                                                                   |                                                                                                                                                 
tGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCActcg                                                                                                                                                 >  1:3459538/1‑150 (MQ=255)
   aaaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAg                                                                                                                                              <  1:84882/150‑1 (MQ=255)
   aaaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAg                                                                                                                                              <  1:1846195/150‑1 (MQ=255)
    aaCGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGt                                                                                                                                             <  1:1276499/150‑1 (MQ=255)
      cGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGa                                                                                                                                           >  2:4699/1‑150 (MQ=255)
           gAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATg                                                                                                                                      <  2:1138859/150‑1 (MQ=255)
           gAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATg                                                                                                                                      <  1:2854325/150‑1 (MQ=255)
            aaGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGt                                                                                                                                     <  2:1478556/150‑1 (MQ=255)
             agagCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTc                                                                                                                                    >  1:1085652/1‑150 (MQ=255)
               agCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTcac                                                                                                                                  >  1:2610077/1‑150 (MQ=255)
                gCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTcaca                                                                                                                                 <  1:876457/150‑1 (MQ=255)
                 ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAg                                                                                                                                >  2:2886884/1‑150 (MQ=255)
                 ccaccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAg                                                                                                                                >  2:3066845/1‑150 (MQ=255)
                  caccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAgg                                                                                                                               <  1:1650434/150‑1 (MQ=255)
                  caccaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAgg                                                                                                                               <  1:633171/150‑1 (MQ=255)
                   accaGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGa                                                                                                                              <  2:1966460/150‑1 (MQ=255)
                          gAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTGCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTa                                                                                                                       <  2:1036422/150‑1 (MQ=255)
                           aaaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                      <  1:141083/150‑1 (MQ=255)
                           aaaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAc                                                                                                                      >  1:938935/1‑150 (MQ=255)
                            aaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                     <  2:9505/150‑1 (MQ=255)
                            aaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                     >  1:2977292/1‑150 (MQ=255)
                            aaCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACt                                                                                                                     >  1:272326/1‑150 (MQ=255)
                             aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACtt                                                                                                                    <  1:2366893/150‑1 (MQ=255)
                             aCTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACta                                                                                                                    <  1:1243529/150‑2 (MQ=255)
                               ttGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGc                                                                                                                  <  1:2318048/150‑1 (MQ=255)
                                tGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGcc                                                                                                                 >  2:2809186/1‑150 (MQ=255)
                                  gTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGc                                                                                                               >  2:1122798/1‑150 (MQ=255)
                                     tttCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAtt                                                                                                            <  2:1902439/150‑1 (MQ=255)
                                        ccTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                         <  2:3635221/150‑1 (MQ=255)
                                        ccTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                         <  1:3600241/150‑1 (MQ=255)
                                         cTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcgc                                                                                                        >  1:3313150/1‑150 (MQ=255)
                                          tGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCt                                                                                                       >  2:2653256/1‑150 (MQ=255)
                                             cTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTaa                                                                                                    >  1:1578582/1‑150 (MQ=255)
                                             cTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTaa                                                                                                    >  1:1785916/1‑150 (MQ=255)
                                              tACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                         <  1:3499273/144‑1 (MQ=255)
                                              tACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTgcg                                                                                                         >  2:3499273/1‑144 (MQ=255)
                                                     gAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGt                                                                                            >  2:1314217/1‑150 (MQ=255)
                                                     gAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGACGGAACAGTACGCCTTGACCTGGCTTTCACTCTAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGt                                                                                            <  2:2094987/150‑1 (MQ=255)
                                                      aGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                           >  2:2616591/1‑150 (MQ=255)
                                                      aGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGtt                                                                                           <  1:3390550/150‑1 (MQ=255)
                                                        ccTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCg                                                                                         <  1:2349027/150‑1 (MQ=255)
                                                         cTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGc                                                                                        <  1:2433490/150‑1 (MQ=255)
                                                         cTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGc                                                                                        >  2:785860/1‑150 (MQ=255)
                                                          tGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGcc                                                                                       >  1:2631919/1‑150 (MQ=255)
                                                              cGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGgc                                                                                   >  1:2046624/1‑150 (MQ=255)
                                                               gTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGgcg                                                                                  >  1:155463/1‑150 (MQ=255)
                                                                 ttACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGcgcg                                                                                <  1:3650443/150‑1 (MQ=255)
                                                                   aCTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGc                                                                              <  2:4083/150‑1 (MQ=255)
                                                                      ggCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACt                                                                           <  2:3034248/150‑1 (MQ=255)
                                                                       gCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTg                                                                          <  2:686986/150‑1 (MQ=255)
                                                                         aaCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGc                                                                        <  1:722043/150‑1 (MQ=255)
                                                                         aaCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGc                                                                        <  2:1979835/150‑1 (MQ=255)
                                                                         aaCATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGc                                                                        >  2:2241677/1‑150 (MQ=255)
                                                                            aTTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgtt                                                                     >  1:1001491/1‑150 (MQ=255)
                                                                            aTTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgtt                                                                     <  2:2665955/150‑1 (MQ=255)
                                                                             ttACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCgttg                                                                    >  1:1927167/1‑150 (MQ=255)
                                                                                cGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGttt                                                                 >  1:1041189/1‑150 (MQ=255)
                                                                                      cGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGga                                                           <  1:977771/150‑1 (MQ=255)
                                                                                      cGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGga                                                           <  1:3252608/150‑1 (MQ=255)
                                                                                       gTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGgag                                                          >  2:2855026/1‑150 (MQ=255)
                                                                                              gTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTg                                                   <  2:2177507/150‑1 (MQ=255)
                                                                                               ttACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTgg                                                  <  1:453054/150‑1 (MQ=255)
                                                                                                 aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGTACTGGCGTTGTTTCAGGGAGATAACTGGCa                                                <  1:2654547/150‑1 (MQ=255)
                                                                                                 aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCa                                                <  1:2723418/150‑1 (MQ=255)
                                                                                                 aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCa                                                <  1:3220697/150‑1 (MQ=255)
                                                                                                 aCATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCCGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCa                                                >  2:2543419/1‑150 (MQ=255)
                                                                                                  cATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAg                                               <  1:3735208/150‑1 (MQ=255)
                                                                                                  cATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAg                                               >  1:250041/1‑150 (MQ=255)
                                                                                                   aTTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAgg                                              >  1:2245487/1‑150 (MQ=255)
                                                                                                   aTTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAgg                                              >  1:3182593/1‑150 (MQ=255)
                                                                                                     tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                            <  1:1393584/150‑1 (MQ=255)
                                                                                                     tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                            >  2:1519201/1‑150 (MQ=255)
                                                                                                     tACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCt                                            <  2:1085652/150‑1 (MQ=255)
                                                                                                      aCCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTc                                           >  1:79467/1‑150 (MQ=255)
                                                                                                         ttGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTg                                        >  1:3669106/1‑150 (MQ=255)
                                                                                                            cgccgccgcCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGaaa                                     >  1:3141819/1‑150 (MQ=255)
                                                                                                            cgccgccgcCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGaaa                                     >  1:1243097/1‑150 (MQ=255)
                                                                                                            cgccgccgcCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGaaa                                     >  2:3250069/1‑150 (MQ=255)
                                                                                                              ccgccgccgGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACa                                   <  2:2308925/150‑1 (MQ=255)
                                                                                                                      ggAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCa                           >  2:1872938/1‑150 (MQ=255)
                                                                                                                      ggAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCa                           >  2:180110/1‑150 (MQ=255)
                                                                                                                       gAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAg                          <  1:722479/150‑1 (MQ=255)
                                                                                                                        aaCAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAgg                         >  1:2334292/1‑150 (MQ=255)
                                                                                                                             tACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTg                    <  1:3804419/150‑1 (MQ=255)
                                                                                                                                 ccGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCat                >  2:2669790/1‑150 (MQ=255)
                                                                                                                                  cGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCata               >  1:2940908/1‑150 (MQ=255)
                                                                                                                                   gTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCatat              <  2:2397937/150‑1 (MQ=255)
                                                                                                                                   gTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCatat              <  2:1132214/150‑1 (MQ=255)
                                                                                                                                       ccTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCg          >  2:3383700/1‑150 (MQ=255)
                                                                                                                                       ccTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCg          >  1:3156628/1‑150 (MQ=255)
                                                                                                                                         tGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCTGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTg        <  1:781717/150‑1 (MQ=255)
                                                                                                                                          ggCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGc       <  1:1066080/150‑1 (MQ=255)
                                                                                                                                           gCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc      <  1:224670/150‑1 (MQ=255)
                                                                                                                                           gCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc      >  2:3209735/1‑150 (MQ=255)
                                                                                                                                           gCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc      >  1:2392787/1‑150 (MQ=255)
                                                                                                                                           gCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGcc      <  1:2133585/150‑1 (MQ=255)
                                                                                                                                            cTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCa     >  2:2287847/1‑150 (MQ=255)
                                                                                                                                              ttCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATc   <  2:3313095/150‑1 (MQ=255)
                                                                                                                                               tCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCg  >  2:75542/1‑150 (MQ=255)
                                                                                                                                               tCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACAGTGTGTCAGGCGTTGGCATCTAGCGTGCCATCg  >  2:2570867/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCG  >  CP009273/1151603‑1151895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCG  >  CP009273/1151601‑1151899
                                                                                                                                                     |                                                                                                                                                     
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACT                                                                                                                                                       <  SRR19973061.3176936/150‑1 (MQ=60)
 CTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTC                                                                                                                                                      <  SRR19973061.1897357/150‑1 (MQ=60)
  TGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCG                                                                                                                                                     >  SRR19973061.3468409/1‑150 (MQ=60)
     AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAG                                                                                                                                                  <  SRR19973061.1850813/150‑1 (MQ=60)
     AAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAG                                                                                                                                                  <  SRR19973061.85179/150‑1 (MQ=60)
      AACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGT                                                                                                                                                 <  SRR19973061.1279644/150‑1 (MQ=60)
       ACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTG                                                                                                                                                <  SRR19973061.3115686/150‑1 (MQ=60)
             GAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATG                                                                                                                                          <  SRR19973061.2861630/150‑1 (MQ=60)
               AGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTC                                                                                                                                        >  SRR19973061.1088433/1‑150 (MQ=60)
               AGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTC                                                                                                                                        <  SRR19973061.2661886/150‑1 (MQ=60)
                 AGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCAC                                                                                                                                      >  SRR19973061.2616783/1‑150 (MQ=60)
                  GCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACA                                                                                                                                     <  SRR19973061.878731/150‑1 (MQ=60)
                   CCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAG                                                                                                                                    >  SRR19973061.2314780/1‑150 (MQ=60)
                    CACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGG                                                                                                                                   <  SRR19973061.786962/150‑1 (MQ=60)
                    CACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGG                                                                                                                                   <  SRR19973061.634784/150‑1 (MQ=60)
                    CACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGG                                                                                                                                   <  SRR19973061.1654581/150‑1 (MQ=60)
                             AAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAC                                                                                                                          <  SRR19973061.141533/150‑1 (MQ=60)
                             AAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTAC                                                                                                                          >  SRR19973061.941321/1‑150 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                         >  SRR19973061.2984975/1‑150 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                         >  SRR19973061.273072/1‑150 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                         >  SRR19973061.2984960/1‑150 (MQ=60)
                              AACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTATCACTCGAAGTGACAATGTCACAGGATGCATTACT                                                                                                                         >  SRR19973061.273101/1‑150 (MQ=60)
                               ACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTT                                                                                                                        >  SRR19973061.1399174/1‑150 (MQ=60)
                               ACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTT                                                                                                                        <  SRR19973061.2372914/150‑1 (MQ=60)
                               ACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTA                                                                                                                        <  SRR19973061.1246613/150‑1 (MQ=60)
                               ACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGCCACAGGATGCATTACTA                                                                                                                        <  SRR19973061.1246575/150‑1 (MQ=60)
                                 TTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGC                                                                                                                      <  SRR19973061.2323932/150‑1 (MQ=60)
                                 TTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTGACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGC                                                                                                                      <  SRR19973061.2324238/150‑1 (MQ=60)
                                  TGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCC                                                                                                                     >  SRR19973061.3321637/1‑150 (MQ=60)
                                   GGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCG                                                                                                                    >  SRR19973061.729511/1‑150 (MQ=60)
                                     TTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCA                                                                                                                  >  SRR19973061.247769/1‑150 (MQ=60)
                                          cgatctTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCG                                                                                                             <  SRR19973061.3508218/144‑1 (MQ=60)
                                          CCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCG                                                                                                             <  SRR19973061.3609459/150‑1 (MQ=60)
                                           CTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGC                                                                                                            >  SRR19973061.3321692/1‑150 (MQ=60)
                                           CTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGC                                                                                                            >  SRR19973061.1874797/1‑150 (MQ=60)
                                            TGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCT                                                                                                           <  SRR19973061.2227743/150‑1 (MQ=60)
                                              GCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTA                                                                                                         <  SRR19973061.2343555/150‑1 (MQ=60)
                                               CTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAA                                                                                                        >  SRR19973061.1582503/1‑150 (MQ=60)
                                               CTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAA                                                                                                        >  SRR19973061.3483720/1‑150 (MQ=60)
                                               CTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAA                                                                                                        >  SRR19973061.1790390/1‑150 (MQ=60)
                                                TACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAG                                                                                                       >  SRR19973061.1069707/1‑150 (MQ=60)
                                                    CGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCC                                                                                                   >  SRR19973061.1502747/1‑150 (MQ=60)
                                                     GCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCG                                                                                                  <  SRR19973061.853978/150‑1 (MQ=60)
                                                        AGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTT                                                                                               <  SRR19973061.3399261/150‑1 (MQ=60)
                                                          CCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCG                                                                                             <  SRR19973061.2354993/150‑1 (MQ=60)
                                                          CCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACACGAAGTGACAATGTCCCAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCG                                                                                             >  SRR19973061.815/1‑150 (MQ=60)
                                                           CTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGC                                                                                            <  SRR19973061.2439699/150‑1 (MQ=60)
                                                            TGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCC                                                                                           >  SRR19973061.2638664/1‑150 (MQ=60)
                                                            TGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCC                                                                                           >  SRR19973061.2638682/1‑150 (MQ=60)
                                                            TGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCC                                                                                           >  SRR19973061.1420561/1‑150 (MQ=60)
                                                             GAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCT                                                                                          <  SRR19973061.935111/150‑1 (MQ=60)
                                                                CGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGC                                                                                       >  SRR19973061.2051767/1‑150 (MQ=60)
                                                                 GTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCG                                                                                      >  SRR19973061.155951/1‑150 (MQ=60)
                                                                 GTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCG                                                                                      >  SRR19973061.311393/1‑150 (MQ=60)
                                                                 GTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCG                                                                                      >  SRR19973061.2135037/1‑150 (MQ=60)
                                                                   TTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCG                                                                                    <  SRR19973061.3659768/150‑1 (MQ=60)
                                                                     ACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGC                                                                                  <  SRR19973061.3779836/150‑1 (MQ=60)
                                                                           AACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGC                                                                            <  SRR19973061.723927/150‑1 (MQ=60)
                                                                           AACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGC                                                                            <  SRR19973061.723219/150‑1 (MQ=60)
                                                                             CATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGT                                                                          <  SRR19973061.583718/150‑1 (MQ=60)
                                                                              ATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCAATGCGCTTAAGCGCCGGTTCGCCAGGCGCGGCACTGGCGTT                                                                         >  SRR19973061.1004042/1‑150 (MQ=60)
                                                                               TTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTG                                                                        >  SRR19973061.1931949/1‑150 (MQ=60)
                                                                                TACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGT                                                                       >  SRR19973061.2364488/1‑150 (MQ=60)
                                                                                  CGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTT                                                                     >  SRR19973061.1043855/1‑150 (MQ=60)
                                                                                     AGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAG                                                                  >  SRR19973061.1626800/1‑150 (MQ=60)
                                                                                        CGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGA                                                               <  SRR19973061.980259/150‑1 (MQ=60)
                                                                                        CGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGA                                                               <  SRR19973061.3260976/150‑1 (MQ=60)
                                                                                         GTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAG                                                              >  SRR19973061.3158325/1‑150 (MQ=60)
                                                                                            GTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATA                                                           <  SRR19973061.1125667/150‑1 (MQ=60)
                                                                                            GTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATA                                                           <  SRR19973061.2402223/150‑1 (MQ=60)
                                                                                                 TTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGG                                                      <  SRR19973061.454277/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGTACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.2661367/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.3230432/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.3228979/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.2730420/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.2730425/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.2660071/150‑1 (MQ=60)
                                                                                                   ACATTACCTTGCGCCGCCGCCGGAACAGGACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCA                                                    <  SRR19973061.1326069/150‑1 (MQ=60)
                                                                                                    CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                   <  SRR19973061.3744764/150‑1 (MQ=60)
                                                                                                    CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                   >  SRR19973061.250743/1‑150 (MQ=60)
                                                                                                    CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                   >  SRR19973061.15740/1‑150 (MQ=60)
                                                                                                    CATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAG                                                   >  SRR19973061.250742/1‑150 (MQ=60)
                                                                                                     ATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCCTTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGG                                                  >  SRR19973061.3190761/1‑150 (MQ=60)
                                                                                                     ATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGG                                                  >  SRR19973061.2251132/1‑150 (MQ=60)
                                                                                                     ATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGG                                                  >  SRR19973061.3190759/1‑150 (MQ=60)
                                                                                                       TACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCT                                                <  SRR19973061.1397108/150‑1 (MQ=60)
                                                                                                       TACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCT                                                <  SRR19973061.1723152/150‑1 (MQ=60)
                                                                                                        ACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTC                                               >  SRR19973061.79752/1‑150 (MQ=60)
                                                                                                           TTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTG                                            >  SRR19973061.3677309/1‑150 (MQ=60)
                                                                                                           TTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTG                                            >  SRR19973061.3678490/1‑150 (MQ=60)
                                                                                                           TTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTG                                            >  SRR19973061.2544497/1‑150 (MQ=60)
                                                                                                            TGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGA                                           <  SRR19973061.3733343/150‑1 (MQ=60)
                                                                                                              CGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAA                                         >  SRR19973061.1269157/1‑150 (MQ=60)
                                                                                                              CGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAA                                         >  SRR19973061.1246179/1‑150 (MQ=60)
                                                                                                              CGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAA                                         >  SRR19973061.3149905/1‑150 (MQ=60)
                                                                                                              CGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAA                                         >  SRR19973061.1028277/1‑150 (MQ=60)
                                                                                                               GCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAAC                                        <  SRR19973061.3165591/150‑1 (MQ=60)
                                                                                                                    CGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATTCATTACTTGCCGCATTGCGATTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGT                                   >  SRR19973061.1842154/1‑150 (MQ=60)
                                                                                                                    CGCCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGT                                   >  SRR19973061.449289/1‑150 (MQ=60)
                                                                                                                      CCGGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGT                                 <  SRR19973061.3417424/150‑1 (MQ=60)
                                                                                                                        GGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCA                               >  SRR19973061.95913/1‑150 (MQ=60)
                                                                                                                        GGAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCA                               >  SRR19973061.3354781/1‑150 (MQ=60)
                                                                                                                         GAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAG                              <  SRR19973061.724366/150‑1 (MQ=60)
                                                                                                                         GAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAG                              <  SRR19973061.1983954/150‑1 (MQ=60)
                                                                                                                         GAACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAG                              <  SRR19973061.724317/150‑1 (MQ=60)
                                                                                                                          AACAGTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGG                             >  SRR19973061.2340220/1‑150 (MQ=60)
                                                                                                                              GTACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTT                         <  SRR19973061.2218570/150‑1 (MQ=60)
                                                                                                                               TACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTG                        <  SRR19973061.3596853/150‑1 (MQ=60)
                                                                                                                               TACGCCGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTG                        <  SRR19973061.3814115/150‑1 (MQ=60)
                                                                                                                                    CGTGACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATA                   >  SRR19973061.2948477/1‑150 (MQ=60)
                                                                                                                                       GACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAG                >  SRR19973061.3627033/1‑150 (MQ=60)
                                                                                                                                       GACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAG                <  SRR19973061.2894283/150‑1 (MQ=60)
                                                                                                                                       GACCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAG                <  SRR19973061.3507726/150‑1 (MQ=60)
                                                                                                                                         CCTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCG              >  SRR19973061.3164741/1‑150 (MQ=60)
                                                                                                                                          CTGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGT             >  SRR19973061.644211/1‑150 (MQ=60)
                                                                                                                                           TGGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCTGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTG            <  SRR19973061.783772/150‑1 (MQ=60)
                                                                                                                                            GGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGC           <  SRR19973061.1068818/150‑1 (MQ=60)
                                                                                                                                            GGCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGC           <  SRR19973061.1068801/150‑1 (MQ=60)
                                                                                                                                             GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC          <  SRR19973061.2138944/150‑1 (MQ=60)
                                                                                                                                             GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC          >  SRR19973061.2398888/1‑150 (MQ=60)
                                                                                                                                             GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC          >  SRR19973061.2398631/1‑150 (MQ=60)
                                                                                                                                             GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC          <  SRR19973061.225326/150‑1 (MQ=60)
                                                                                                                                             GCTTTCACTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCC          <  SRR19973061.225327/150‑1 (MQ=60)
                                                                                                                                                    CTCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGC   >  SRR19973061.1490890/1‑150 (MQ=60)
                                                                                                                                                     TCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCG  <  SRR19973061.3242275/150‑1 (MQ=60)
                                                                                                                                                     TCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCG  <  SRR19973061.3074765/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCAACATTACGTAGTCGTTGTCGGTTACATTACCTTGCGCCGCCGCCGGAACAGTACGCCGTGACCTGGCTTTCACGCGAAGTGACAATGTCACAGGATGCATTACTTGCCGCATTGCGCTTAAGCGCCGGTTCGCCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCG  >  CP009273/1151601‑1151899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑