Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,193,873 G→T 100% intergenic (‑180/‑278) ymfE ← / → lit e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,193,8730GT98.2% 459.4 / ‑0.7 127intergenic (‑180/‑278)ymfE/lite14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (77/47);  total (78/49)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 131 total aligned reads displayed.

ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCC  >  CP009273/1193727‑1194018
                                                                                                                                                  |                                                                                                                                                 
atTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTtaga                                                                                                                                                >  1:1211576/1‑150 (MQ=255)
atTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTgaga                                                                                                                                                <  2:1866221/150‑1 (MQ=255)
 tttAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCTCGTATCGAGGAGGAGTAGCGCTCCAAATTTGACGCTGGGTTAGaa                                                                                                                                               >  2:498041/1‑150 (MQ=255)
 tttAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGaa                                                                                                                                               >  1:4718063/1‑150 (MQ=255)
   taattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACACTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATc                                                                                                                                             >  2:1894292/1‑150 (MQ=255)
   taattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATc                                                                                                                                             >  1:1150032/1‑150 (MQ=255)
   taattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATc                                                                                                                                             >  1:3577297/1‑150 (MQ=255)
   taattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATc                                                                                                                                             >  1:3576720/1‑150 (MQ=255)
   taattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATc                                                                                                                                             >  2:1894732/1‑150 (MQ=255)
    aattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCt                                                                                                                                            <  1:451487/150‑1 (MQ=255)
      ttaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGa                                                                                                                                          <  1:671973/150‑1 (MQ=255)
        aaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTCCAAATTTGACGCTGGGTTAGACTCTGaaa                                                                                                                                        >  2:4195554/1‑150 (MQ=255)
        aaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaaa                                                                                                                                        >  1:3282120/1‑150 (MQ=255)
        aaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaaa                                                                                                                                        >  2:4195541/1‑150 (MQ=255)
         aGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAt                                                                                                                                       <  2:2379739/150‑1 (MQ=255)
         aGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAt                                                                                                                                       >  2:4447962/1‑150 (MQ=255)
           tgtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTg                                                                                                                                     <  2:1005669/150‑1 (MQ=255)
             tCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAt                                                                                                                                   >  1:3506592/1‑150 (MQ=255)
             tCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAt                                                                                                                                   <  1:1728185/150‑1 (MQ=255)
                ttATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAga                                                                                                                                >  2:1626266/1‑150 (MQ=255)
                 tatataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaa                                                                                                                               >  2:702128/1‑150 (MQ=255)
                 tatataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaa                                                                                                                               <  2:3282120/150‑1 (MQ=255)
                  atataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaa                                                                                                                              <  1:1365009/150‑1 (MQ=255)
                  atataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaa                                                                                                                              >  1:2823180/1‑150 (MQ=255)
                   tataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaat                                                                                                                             <  1:381760/150‑1 (MQ=255)
                    ataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatg                                                                                                                            <  1:1756604/150‑1 (MQ=255)
                          ttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataa                                                                                                                      <  1:1667607/150‑1 (MQ=255)
                           tCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataat                                                                                                                     >  2:3903161/1‑150 (MQ=255)
                            cAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatg                                                                                                                    <  1:1804957/150‑1 (MQ=255)
                            cAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatg                                                                                                                    <  1:1804743/150‑1 (MQ=255)
                            cAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatg                                                                                                                    <  1:1804557/150‑1 (MQ=255)
                            cAGTAGGTTAGGTACAATTTTTTGTATCTGTTCAGGCTGACCGAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatg                                                                                                                    <  1:3471749/150‑1 (MQ=255)
                              gTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaa                                                                                                                  >  2:4501347/1‑150 (MQ=255)
                              gTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaa                                                                                                                  >  2:4501359/1‑150 (MQ=255)
                               tAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaa                                                                                                                 >  2:2033294/1‑150 (MQ=255)
                                    tagttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatg                                                                                                            >  1:4586043/1‑150 (MQ=255)
                                    tagttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatg                                                                                                            >  1:3284900/1‑150 (MQ=255)
                                         aCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATaatgaaatg                                                                                                       >  1:1799165/1‑150 (MQ=255)
                                          cAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATaatgaaatga                                                                                                      <  2:3323676/150‑1 (MQ=255)
                                           aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                     >  1:3037858/1‑150 (MQ=255)
                                           aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                     >  1:3729684/1‑150 (MQ=255)
                                           aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                     <  2:3459284/150‑1 (MQ=255)
                                           aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                     >  1:2185142/1‑150 (MQ=255)
                                             ttttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatga                                                                                                   <  2:990716/150‑1 (MQ=255)
                                               ttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaa                                                                                                 >  2:4520752/1‑150 (MQ=255)
                                                   gTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                             <  2:3576720/150‑1 (MQ=255)
                                                   gTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                             <  2:3577297/150‑1 (MQ=255)
                                                    tATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatg                                                                                            <  2:2249949/150‑1 (MQ=255)
                                                    tATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatg                                                                                            <  2:2249248/150‑1 (MQ=255)
                                                     aTCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatga                                                                                           >  1:1467478/1‑150 (MQ=255)
                                                     aTCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatga                                                                                           >  1:1467663/1‑150 (MQ=255)
                                                         gTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatgaaatgatgaaatg                                                                                       >  1:2908034/1‑150 (MQ=255)
                                                           tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgat                                                                                     >  1:2331422/1‑150 (MQ=255)
                                                           tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgat                                                                                     >  1:2330258/1‑150 (MQ=255)
                                                           tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgat                                                                                     >  2:1412178/1‑150 (MQ=255)
                                                             aGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatga                                                                                   >  1:16984/1‑150 (MQ=255)
                                                             aGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatga                                                                                   >  1:4458747/1‑150 (MQ=255)
                                                                 tGACCTAGCTTCGCTGACCGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatg                                                                               >  2:4281601/1‑150 (MQ=255)
                                                                          ttCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatga                                                                      <  1:1000744/150‑1 (MQ=255)
                                                                                 acagacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatg                                                               >  2:4646869/1‑150 (MQ=255)
                                                                                   agacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                             >  1:2183552/1‑150 (MQ=255)
                                                                                   agacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                             <  2:3829296/150‑1 (MQ=255)
                                                                                   agacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                             >  1:713534/1‑150 (MQ=255)
                                                                                   agacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                             <  1:559269/150‑1 (MQ=255)
                                                                                     acaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatga                                                           >  2:352205/1‑150 (MQ=255)
                                                                                      caATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCGGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAc                                                          <  1:594847/150‑1 (MQ=255)
                                                                                       aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                         >  1:2912408/1‑150 (MQ=255)
                                                                                       aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                         >  1:539450/1‑150 (MQ=255)
                                                                                       aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                         >  1:539410/1‑150 (MQ=255)
                                                                                           ttGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgt                                                     <  1:3719866/150‑1 (MQ=255)
                                                                                                 tCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAg                                               >  2:2604885/1‑150 (MQ=255)
                                                                                                 tCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAg                                               >  1:1194253/1‑150 (MQ=255)
                                                                                                 tCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAg                                               >  1:3588142/1‑150 (MQ=255)
                                                                                                      aGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGc                                          >  2:304254/1‑150 (MQ=255)
                                                                                                       gCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCa                                         >  2:2092437/1‑150 (MQ=255)
                                                                                                        cACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCAc                                        <  1:308516/150‑1 (MQ=255)
                                                                                                        cACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCAc                                        >  2:1896847/1‑150 (MQ=255)
                                                                                                        cACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCAc                                        >  2:1898120/1‑150 (MQ=255)
                                                                                                        cACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCAc                                        >  2:2060970/1‑150 (MQ=255)
                                                                                                         aCGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACg                                       >  1:2084383/1‑150 (MQ=255)
                                                                                                            tATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGAt                                    <  2:2650505/150‑1 (MQ=255)
                                                                                                            tATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGAt                                    <  1:3074166/150‑1 (MQ=255)
                                                                                                                gaggaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGgtg                                <  2:791288/150‑1 (MQ=255)
                                                                                                                   gaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                             >  1:3258964/1‑150 (MQ=255)
                                                                                                                   gaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                             >  1:3258829/1‑150 (MQ=255)
                                                                                                                      gagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTAc                          <  1:2492034/150‑1 (MQ=255)
                                                                                                                              cTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCt                  >  1:2006781/1‑150 (MQ=255)
                                                                                                                               tACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCtt                 >  2:1642955/1‑150 (MQ=255)
                                                                                                                                aCAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTa                <  1:4444762/150‑1 (MQ=255)
                                                                                                                                aCAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTa                <  1:2379268/150‑1 (MQ=255)
                                                                                                                                  aaaTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTAcc              >  2:2918849/1‑150 (MQ=255)
                                                                                                                                   aaTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCt             <  2:3342785/150‑1 (MQ=255)
                                                                                                                                      ttGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTaa          >  1:1643644/1‑150 (MQ=255)
                                                                                                                                        gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc        <  2:1704322/150‑1 (MQ=255)
                                                                                                                                        gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc        <  2:1194253/150‑1 (MQ=255)
                                                                                                                                        gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc        >  1:958154/1‑150 (MQ=255)
                                                                                                                                         aCGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCg       <  2:4148999/150‑1 (MQ=255)
                                                                                                                                         aCGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCg       <  2:4148855/150‑1 (MQ=255)
                                                                                                                                          cGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGt      <  2:1150032/150‑1 (MQ=255)
                                                                                                                                          cGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGt      >  2:3264788/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCC  >  CP009273/1193727‑1194018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  >  CP009273/1193727‑1194022
                                                                                                                                                  |                                                                                                                                                     
ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGA                                                                                                                                                    >  SRR19973082.1219768/1‑150 (MQ=60)
ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGA                                                                                                                                                    >  SRR19973082.1220025/1‑150 (MQ=60)
 TTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAA                                                                                                                                                   >  SRR19973082.4746456/1‑150 (MQ=60)
   TAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATC                                                                                                                                                 >  SRR19973082.1157626/1‑150 (MQ=60)
   TAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATC                                                                                                                                                 >  SRR19973082.3598466/1‑150 (MQ=60)
   TAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATC                                                                                                                                                 >  SRR19973082.3599048/1‑150 (MQ=60)
   TAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATC                                                                                                                                                 >  SRR19973082.362918/1‑150 (MQ=60)
    AATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCT                                                                                                                                                <  SRR19973082.454620/150‑1 (MQ=60)
      TTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGA                                                                                                                                              <  SRR19973082.676614/150‑1 (MQ=60)
      TTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGA                                                                                                                                              <  SRR19973082.676587/150‑1 (MQ=60)
        AAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAA                                                                                                                                            >  SRR19973082.3302377/1‑150 (MQ=60)
        AAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAA                                                                                                                                            >  SRR19973082.3302396/1‑150 (MQ=60)
            GTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGA                                                                                                                                        >  SRR19973082.1711433/1‑150 (MQ=60)
             TCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAT                                                                                                                                       <  SRR19973082.1739661/150‑1 (MQ=60)
             TCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAT                                                                                                                                       >  SRR19973082.3528009/1‑150 (MQ=60)
                  ATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAA                                                                                                                                  >  SRR19973082.2840468/1‑150 (MQ=60)
                  ATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAA                                                                                                                                  <  SRR19973082.1374418/150‑1 (MQ=60)
                   TATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAAT                                                                                                                                 <  SRR19973082.384189/150‑1 (MQ=60)
                   TATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAAT                                                                                                                                 <  SRR19973082.4029251/150‑1 (MQ=60)
                    ATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATG                                                                                                                                <  SRR19973082.1768241/150‑1 (MQ=60)
                          TTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAA                                                                                                                          <  SRR19973082.1678704/150‑1 (MQ=60)
                            CAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATG                                                                                                                        <  SRR19973082.1816589/150‑1 (MQ=60)
                            CAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATG                                                                                                                        <  SRR19973082.1816805/150‑1 (MQ=60)
                            CAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATG                                                                                                                        <  SRR19973082.1816400/150‑1 (MQ=60)
                            CAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATG                                                                                                                        <  SRR19973082.557271/150‑1 (MQ=60)
                            CAGTAGGTTAGGTACAATTTTTTGTATCTGTTCAGGCTGACCGAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATG                                                                                                                        <  SRR19973082.3493063/150‑1 (MQ=60)
                                    TAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATG                                                                                                                >  SRR19973082.2219814/1‑150 (MQ=60)
                                    TAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATG                                                                                                                >  SRR19973082.4613883/1‑150 (MQ=60)
                                    TAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATG                                                                                                                >  SRR19973082.3305174/1‑150 (MQ=60)
                                         ACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATG                                                                                                           >  SRR19973082.1810985/1‑150 (MQ=60)
                                         ACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATG                                                                                                           >  SRR19973082.1811019/1‑150 (MQ=60)
                                           AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                                         >  SRR19973082.3056537/1‑150 (MQ=60)
                                           AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                                         >  SRR19973082.3752759/1‑150 (MQ=60)
                                           AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                                         >  SRR19973082.2199000/1‑150 (MQ=60)
                                                     ATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGA                                                                                               >  SRR19973082.1477368/1‑150 (MQ=60)
                                                     ATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGA                                                                                               >  SRR19973082.1477554/1‑150 (MQ=60)
                                                         GTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATG                                                                                           >  SRR19973082.2926005/1‑150 (MQ=60)
                                                           TCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGAT                                                                                         >  SRR19973082.2345853/1‑150 (MQ=60)
                                                           TCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGAT                                                                                         >  SRR19973082.2344682/1‑150 (MQ=60)
                                                             AGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGA                                                                                       >  SRR19973082.4485835/1‑150 (MQ=60)
                                                             AGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGA                                                                                       >  SRR19973082.17229/1‑150 (MQ=60)
                                                                  GACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGA                                                                                  >  SRR19973082.1638401/1‑150 (MQ=60)
                                                                          TTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGA                                                                          <  SRR19973082.1007713/150‑1 (MQ=60)
                                                                                   AGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGAT                                                                 >  SRR19973082.2197394/1‑150 (MQ=60)
                                                                                   AGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGAT                                                                 <  SRR19973082.563266/150‑1 (MQ=60)
                                                                                   AGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGAT                                                                 >  SRR19973082.718321/1‑150 (MQ=60)
                                                                                      CAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCGGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAC                                                              <  SRR19973082.599080/150‑1 (MQ=60)
                                                                                       AATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACA                                                             >  SRR19973082.543286/1‑150 (MQ=60)
                                                                                       AATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACA                                                             >  SRR19973082.543326/1‑150 (MQ=60)
                                                                                       AATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACA                                                             >  SRR19973082.2930409/1‑150 (MQ=60)
                                                                                       AATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACA                                                             >  SRR19973082.2930355/1‑150 (MQ=60)
                                                                                           TTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGT                                                         <  SRR19973082.3742886/150‑1 (MQ=60)
                                                                                                 TCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAG                                                   >  SRR19973082.3610030/1‑150 (MQ=60)
                                                                                                 TCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAG                                                   >  SRR19973082.1202231/1‑150 (MQ=60)
                                                                                                       GCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCA                                             <  SRR19973082.4649936/150‑1 (MQ=60)
                                                                                                        CACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCAC                                            <  SRR19973082.310695/150‑1 (MQ=60)
                                                                                                         ACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACG                                           >  SRR19973082.2468809/1‑150 (MQ=60)
                                                                                                         ACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACG                                           >  SRR19973082.2097653/1‑150 (MQ=60)
                                                                                                            TATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGAT                                        <  SRR19973082.3092966/150‑1 (MQ=60)
                                                                                                                   GAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCT                                 >  SRR19973082.3279013/1‑150 (MQ=60)
                                                                                                                   GAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCT                                 >  SRR19973082.3278878/1‑150 (MQ=60)
                                                                                                                    AGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTT                                <  SRR19973082.2046201/150‑1 (MQ=60)
                                                                                                                      GAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTAC                              <  SRR19973082.2507589/150‑1 (MQ=60)
                                                                                                                         TAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGAC                           >  SRR19973082.3578923/1‑150 (MQ=60)
                                                                                                                              CTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCT                      >  SRR19973082.2019444/1‑150 (MQ=60)
                                                                                                                               TACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTT                     <  SRR19973082.3116064/150‑1 (MQ=60)
                                                                                                                                ACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTA                    <  SRR19973082.2393853/150‑1 (MQ=60)
                                                                                                                                ACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTA                    <  SRR19973082.4471748/150‑1 (MQ=60)
                                                                                                                                      TTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAA              >  SRR19973082.1654568/1‑150 (MQ=60)
                                                                                                                                        GACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC            >  SRR19973082.964818/1‑150 (MQ=60)
                                                                                                                                                  TAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  >  SRR19973082.2262972/1‑150 (MQ=60)
                                                                                                                                                  TAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  >  SRR19973082.2262998/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  >  CP009273/1193727‑1194022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑