Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,193,873 G→T 85.6% intergenic (‑180/‑278) ymfE ← / → lit e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,193,8730GT85.6% 375.5 / 60.7 152intergenic (‑180/‑278)ymfE/lite14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
Reads supporting (aligned to +/- strand):  ref base G (13/9);  new base T (48/82);  total (61/91)
Fisher's exact test for biased strand distribution p-value = 6.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGT  >  CP009273/1193727‑1194021
                                                                                                                                                  |                                                                                                                                                    
 tttAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGaa                                                                                                                                                  >  2:3877617/1‑150 (MQ=255)
  ttaattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTCCAAATTTGACGCTGGGTTAGAAt                                                                                                                                                 >  1:934006/1‑150 (MQ=255)
  ttaattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAt                                                                                                                                                 >  1:933994/1‑150 (MQ=255)
  ttaattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAt                                                                                                                                                 <  1:1726673/150‑1 (MQ=255)
    aattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCt                                                                                                                                               <  1:2505403/150‑1 (MQ=255)
    aattaaGTGTCATTATATACTTTTCAGGAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCt                                                                                                                                               <  2:960524/150‑1 (MQ=255)
     attaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACCGACAATATTGTGATCAGTAGCACGTATCGCGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTg                                                                                                                                              >  1:1232799/1‑150 (MQ=255)
     attaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTg                                                                                                                                              >  1:2526650/1‑150 (MQ=255)
     attaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTg                                                                                                                                              <  1:1302951/150‑1 (MQ=255)
       taaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTGGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaa                                                                                                                                            >  1:2173137/1‑150 (MQ=255)
       taaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaa                                                                                                                                            <  1:5055552/150‑1 (MQ=255)
       taaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaa                                                                                                                                            <  1:1324170/150‑1 (MQ=255)
       taaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaa                                                                                                                                            >  1:2173291/1‑150 (MQ=255)
        aaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGaaa                                                                                                                                           >  2:1690373/1‑150 (MQ=255)
         aGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAAt                                                                                                                                          <  2:1480581/150‑1 (MQ=255)
           tgtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTg                                                                                                                                        <  1:554407/150‑1 (MQ=255)
           tgtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTg                                                                                                                                        >  2:4274076/1‑150 (MQ=255)
            gtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGa                                                                                                                                       >  2:4956784/1‑150 (MQ=255)
             tCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAt                                                                                                                                      >  2:313603/1‑150 (MQ=255)
             tCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAt                                                                                                                                      <  2:1145306/150‑1 (MQ=255)
              cATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATa                                                                                                                                     <  2:247834/150‑1 (MQ=255)
                 tatataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaa                                                                                                                                  <  1:590882/150‑1 (MQ=255)
                   tataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTCCAAATTTGACGCTGGGTGAGAATCTGAAATTGATAgaaat                                                                                                                                >  2:4474740/1‑150 (MQ=255)
                   tataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaat                                                                                                                                <  2:1840584/150‑1 (MQ=255)
                   tataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaat                                                                                                                                <  2:1840570/150‑1 (MQ=255)
                   tataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAgaaat                                                                                                                                <  2:2002076/150‑1 (MQ=255)
                     taCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatga                                                                                                                              >  1:1158899/1‑150 (MQ=255)
                      aCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaa                                                                                                                             <  1:1521052/150‑1 (MQ=255)
                         tttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaata                                                                                                                          <  2:3897783/150‑1 (MQ=255)
                         tttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAaatgaaata                                                                                                                          <  2:1352273/150‑1 (MQ=255)
                           tCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataat                                                                                                                        >  2:1220319/1‑150 (MQ=255)
                            cAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatg                                                                                                                       <  2:3442389/150‑1 (MQ=255)
                            cAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatg                                                                                                                       <  1:2038603/150‑1 (MQ=255)
                               tAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGACTCTGAAATTGATAGAaatgaaataatgaaa                                                                                                                    >  2:3711763/1‑150 (MQ=255)
                               tAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaa                                                                                                                    >  2:4777611/1‑150 (MQ=255)
                               tAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaa                                                                                                                    >  2:4522907/1‑150 (MQ=255)
                                aGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaat                                                                                                                   <  2:594912/150‑1 (MQ=255)
                                  gttagttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataa                                                                                                                 <  2:11916/150‑1 (MQ=255)
                                   ttagttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataat                                                                                                                <  2:1659487/150‑1 (MQ=255)
                                   tnagttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAaacgaaataatgaaataat                                                                                                                >  1:2021584/1‑150 (MQ=255)
                                    tagttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatg                                                                                                               <  1:1690373/150‑1 (MQ=255)
                                     agttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatga                                                                                                              <  1:3557489/150‑1 (MQ=255)
                                      gttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaa                                                                                                             >  2:2660975/1‑150 (MQ=255)
                                      gttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaa                                                                                                             <  2:1150672/150‑1 (MQ=255)
                                      gttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATAGTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaa                                                                                                             >  1:2180477/1‑150 (MQ=255)
                                       ttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaaa                                                                                                            <  2:1457210/150‑1 (MQ=255)
                                        taCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaact                                                                                                           >  1:3378454/1‑148 (MQ=255)
                                        taCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaaat                                                                                                           >  2:4708021/1‑150 (MQ=255)
                                           aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                        >  1:673813/1‑150 (MQ=255)
                                              tttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaa                                                                                                     <  1:2989126/150‑1 (MQ=255)
                                                tttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaat                                                                                                   <  2:1728319/150‑1 (MQ=255)
                                                 ttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatg                                                                                                  >  1:1918464/1‑150 (MQ=255)
                                                  tGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatga                                                                                                 <  2:72418/150‑1 (MQ=255)
                                                  tGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatga                                                                                                 >  2:3137750/1‑150 (MQ=255)
                                                           tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgat                                                                                        <  2:4648773/150‑1 (MQ=255)
                                                                   aCCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                <  1:3414540/150‑1 (MQ=255)
                                                                        gCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaat                                                                           >  1:4709544/1‑150 (MQ=255)
                                                                        gCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaat                                                                           >  2:2822445/1‑150 (MQ=255)
                                                                               tGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                                    <  2:4771127/150‑1 (MQ=255)
                                                                               tGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                                    <  2:893244/150‑1 (MQ=255)
                                                                                 acagacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatg                                                                  >  1:4052458/1‑150 (MQ=255)
                                                                                 acagacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatg                                                                  >  2:3783523/1‑150 (MQ=255)
                                                                                  cagacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAAtgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatga                                                                 <  1:2625403/150‑1 (MQ=255)
                                                                                          atTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACagag                                                         <  2:2526650/150‑1 (MQ=255)
                                                                                            tgtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                                       <  1:4615934/150‑1 (MQ=255)
                                                                                            tgtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                                       <  2:2069307/150‑1 (MQ=255)
                                                                                            tgtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                                       <  1:4615929/150‑1 (MQ=255)
                                                                                            tgtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                                       <  1:2718564/150‑1 (MQ=255)
                                                                                              tgATCAGTGGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTc                                                     <  2:2173137/150‑1 (MQ=255)
                                                                                               gATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTcc                                                    <  2:1256759/150‑1 (MQ=255)
                                                                                                  cAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGt                                                 <  2:1986983/150‑1 (MQ=255)
                                                                                                  cAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGt                                                 <  2:1986950/150‑1 (MQ=255)
                                                                                                             aTCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATTATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATTAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATg                                      <  2:1490864/150‑1 (MQ=255)
                                                                                                              tCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAATGATATAAATGAAATAATGAAAAAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAAGAGTGTCCAGATGGCACGGATgg                                     >  2:2095752/1‑150 (MQ=38)
                                                                                                               cGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGgt                                    <  1:4956784/150‑1 (MQ=255)
                                                                                                                    aggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                               <  2:2296784/150‑1 (MQ=255)
                                                                                                                    aggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                               <  2:332288/150‑1 (MQ=255)
                                                                                                                     ggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTa                              <  1:3877617/150‑1 (MQ=255)
                                                                                                                       agTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACg                            <  1:1681935/150‑1 (MQ=255)
                                                                                                                         tAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGAc                          >  1:2204923/1‑150 (MQ=255)
                                                                                                                             gcTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGc                      >  2:498818/1‑150 (MQ=255)
                                                                                                                              cTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCt                     <  2:2819971/150‑1 (MQ=255)
                                                                                                                               tACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCtt                    <  1:4708036/150‑1 (MQ=255)
                                                                                                                                 cAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTAc                  <  2:338191/150‑1 (MQ=255)
                                                                                                                                   aaTTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTAACCt                >  2:3301825/1‑150 (MQ=255)
                                                                                                                                    aTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCtt               <  2:5111447/150‑1 (MQ=255)
                                                                                                                                    aTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCtt               <  2:5111249/150‑1 (MQ=255)
                                                                                                                                      ttGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTaa             <  1:2905658/150‑1 (MQ=255)
                                                                                                                                      ttGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTaa             >  2:2132682/1‑150 (MQ=255)
                                                                                                                                         aCGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCg          >  1:2849129/1‑150 (MQ=255)
                                                                                                                                           gCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATTAAATGATGAAATGATGAAATGATGAAATGATGAAATAGTGTACATTGGGCAAGTATGGTGTCTTACGAAATGCTTAACTTAATCGtt        >  1:1573925/1‑150 (MQ=37)
                                                                                                                                           gCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGtt        >  1:4762418/1‑150 (MQ=255)
                                                                                                                                            cTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTc       <  2:2079624/150‑1 (MQ=255)
                                                                                                                                            cTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTc       <  2:1693679/150‑1 (MQ=255)
                                                                                                                                            cTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTc       <  1:2817916/150‑1 (MQ=255)
                                                                                                                                            cTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTc       >  1:749389/1‑150 (MQ=255)
                                                                                                                                              gggTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTccc     <  1:3426354/150‑1 (MQ=255)
                                                                                                                                               ggTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCa    <  1:243200/150‑1 (MQ=255)
                                                                                                                                               ggTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCa    >  2:2727114/1‑150 (MQ=255)
                                                                                                                                                 tGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAAAGATGAAATGATGAAATTAAGAAATTATGAAATGAATAAATGATGACACAGAGTCCAGAGGGCACGGATGGTGTCTTACGACATGCTTAACATAATATTTCCCAgt  >  2:512862/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGT  >  CP009273/1193727‑1194021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  >  CP009273/1193725‑1194022
                                                                                                                                                    |                                                                                                                                                     
ATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTA                                                                                                                                                      >  SRR19973077.46674/1‑150 (MQ=60)
ATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTA                                                                                                                                                      >  SRR19973077.46649/1‑150 (MQ=60)
    TTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTCCAAATTTGACGCTGGGTTAGAAT                                                                                                                                                  >  SRR19973077.935668/1‑150 (MQ=60)
    TTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAT                                                                                                                                                  >  SRR19973077.935656/1‑150 (MQ=60)
    TTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAT                                                                                                                                                  <  SRR19973077.1729642/150‑1 (MQ=60)
      AATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCT                                                                                                                                                <  SRR19973077.2509833/150‑1 (MQ=60)
       ATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACCGACAATATTGTGATCAGTAGCACGTATCGCGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTG                                                                                                                                               >  SRR19973077.1234941/1‑150 (MQ=60)
       ATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTG                                                                                                                                               >  SRR19973077.2531124/1‑150 (MQ=60)
       ATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTG                                                                                                                                               <  SRR19973077.1305227/150‑1 (MQ=60)
         TAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTGGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAA                                                                                                                                             >  SRR19973077.2176889/1‑150 (MQ=60)
         TAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAA                                                                                                                                             <  SRR19973077.1326499/150‑1 (MQ=60)
         TAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAA                                                                                                                                             <  SRR19973077.5064518/150‑1 (MQ=60)
         TAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAA                                                                                                                                             >  SRR19973077.2177044/1‑150 (MQ=60)
          AAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAA                                                                                                                                            <  SRR19973077.362734/150‑1 (MQ=60)
             TGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTG                                                                                                                                         <  SRR19973077.555403/150‑1 (MQ=60)
               TCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGAT                                                                                                                                       <  SRR19973077.448295/150‑1 (MQ=60)
                   TATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAA                                                                                                                                   <  SRR19973077.592046/150‑1 (MQ=60)
                   TATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAA                                                                                                                                   <  SRR19973077.591941/150‑1 (MQ=60)
                      ATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATG                                                                                                                                <  SRR19973077.76974/150‑1 (MQ=60)
                       TACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGA                                                                                                                               >  SRR19973077.1160925/1‑150 (MQ=60)
                        ACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAA                                                                                                                              <  SRR19973077.1523703/150‑1 (MQ=60)
                              CAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATG                                                                                                                        <  SRR19973077.2042146/150‑1 (MQ=60)
                                GTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAA                                                                                                                      >  SRR19973077.1369664/1‑150 (MQ=60)
                                 TAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAA                                                                                                                     <  SRR19973077.127677/150‑1 (MQ=60)
                                     TNAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAACGAAATAATGAAATAAT                                                                                                                 >  SRR19973077.2025102/1‑150 (MQ=60)
                                      TAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATG                                                                                                                <  SRR19973077.1693723/150‑1 (MQ=60)
                                      TAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATG                                                                                                                <  SRR19973077.1693284/150‑1 (MQ=60)
                                       AGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGA                                                                                                               <  SRR19973077.3563794/150‑1 (MQ=60)
                                        GTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAA                                                                                                              >  SRR19973077.2184244/1‑150 (MQ=60)
                                        GTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATAGTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAA                                                                                                              >  SRR19973077.2184248/1‑150 (MQ=60)
                                          TACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAACT                                                                                                            >  SRR19973077.3384390/1‑150 (MQ=60)
                                             AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                                         >  SRR19973077.675012/1‑150 (MQ=60)
                                              ATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATG                                                                                                        <  SRR19973077.1662917/150‑1 (MQ=60)
                                                TTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAA                                                                                                      <  SRR19973077.2994394/150‑1 (MQ=60)
                                                   TTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATG                                                                                                   >  SRR19973077.1921827/1‑150 (MQ=60)
                                                        TCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAA                                                                                              <  SRR19973077.4770863/150‑1 (MQ=60)
                                                          TGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAAT                                                                                            <  SRR19973077.2171695/150‑1 (MQ=60)
                                                           GTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATG                                                                                           <  SRR19973077.2001152/150‑1 (MQ=60)
                                                                     ACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGAT                                                                                 <  SRR19973077.3420542/150‑1 (MQ=60)
                                                                          GCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAAT                                                                            >  SRR19973077.4717838/1‑150 (MQ=60)
                                                                          GCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAAT                                                                            >  SRR19973077.4717795/1‑150 (MQ=60)
                                                                                   ACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATG                                                                   >  SRR19973077.4059620/1‑150 (MQ=60)
                                                                                    CAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGA                                                                  <  SRR19973077.2630061/150‑1 (MQ=60)
                                                                                              TGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTG                                                        <  SRR19973077.2723499/150‑1 (MQ=60)
                                                                                              TGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTG                                                        <  SRR19973077.2723410/150‑1 (MQ=60)
                                                                                              TGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTG                                                        <  SRR19973077.4624060/150‑1 (MQ=60)
                                                                                              TGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTG                                                        <  SRR19973077.4624065/150‑1 (MQ=60)
                                                                                               GTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGT                                                       >  SRR19973077.3029505/1‑150 (MQ=60)
                                                                                                         GCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCA                                             >  SRR19973077.261234/1‑150 (MQ=60)
                                                                                                                 CGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGT                                     <  SRR19973077.4965599/150‑1 (MQ=60)
                                                                                                                   AGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGT                                   <  SRR19973077.511047/150‑1 (MQ=60)
                                                                                                                       GGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTA                               <  SRR19973077.3884430/150‑1 (MQ=60)
                                                                                                                         AGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACG                             <  SRR19973077.1684836/150‑1 (MQ=60)
                                                                                                                           TAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGAC                           >  SRR19973077.2208919/1‑150 (MQ=60)
                                                                                                                           TAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGAC                           >  SRR19973077.2208768/1‑150 (MQ=60)
                                                                                                                                 TACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTT                     <  SRR19973077.4716313/150‑1 (MQ=60)
                                                                                                                                 TACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTT                     <  SRR19973077.4716329/150‑1 (MQ=60)
                                                                                                                                        TTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAA              <  SRR19973077.2910802/150‑1 (MQ=60)
                                                                                                                                           ACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCG           >  SRR19973077.2854184/1‑150 (MQ=60)
                                                                                                                                           ACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCG           >  SRR19973077.2854207/1‑150 (MQ=60)
                                                                                                                                             GCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATTAAATGATGAAATGATGAAATGATGAAATGATGAAATAGTGTACATTGGGCAAGTATGGTGTCTTACGAAATGCTTAACTTAATCGTT         >  SRR19973077.1576670/1‑150 (MQ=60)
                                                                                                                                             GCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTT         >  SRR19973077.4770837/1‑150 (MQ=60)
                                                                                                                                              CTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTC        >  SRR19973077.750704/1‑150 (MQ=60)
                                                                                                                                              CTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTC        <  SRR19973077.2822931/150‑1 (MQ=60)
                                                                                                                                                GGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCC      <  SRR19973077.3432369/150‑1 (MQ=60)
                                                                                                                                                GGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCC      <  SRR19973077.3432159/150‑1 (MQ=60)
                                                                                                                                                 GGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCA     <  SRR19973077.243651/150‑1 (MQ=60)
                                                                                                                                                 GGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCA     <  SRR19973077.243902/150‑1 (MQ=60)
                                                                                                                                                   TTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGT   <  SRR19973077.3558821/150‑1 (MQ=60)
                                                                                                                                                    TAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  <  SRR19973077.2665713/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTG  >  CP009273/1193725‑1194022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑