Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F159 I0 R1	879	305.9	9588012	96.6%	9262019	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,193,873	G→T	93.3%	intergenic (‑180/‑278)	ymfE ← / → lit	e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,193,873	0	G	T	93.3%	263.5 / 12.2	90	intergenic (‑180/‑278)	ymfE/lit	e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
Reads supporting (aligned to +/- strand): ref base G (4/2); new base T (38/46); total (42/48)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

TTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC  >  CP009273/1193728‑1194012

tttAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGaa                                                                                                                                         <  2:1606399/150‑1 (MQ=255)

tttAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGaa                                                                                                                                         <  2:4009814/150‑1 (MQ=255)

 ttaattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAt                                                                                                                                        <  2:4409950/150‑1 (MQ=255)

 ttaattaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAt                                                                                                                                        <  1:2392164/150‑1 (MQ=255)

    attaaGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTg                                                                                                                                     >  1:4430473/1‑150 (MQ=255)

        aGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAt                                                                                                                                 >  1:1242670/1‑150 (MQ=255)

         gtgtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAtt                                                                                                                                <  1:357508/150‑1 (MQ=255)

         gtgtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAtt                                                                                                                                <  2:1243989/150‑1 (MQ=255)

         gtgtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAtt                                                                                                                                <  2:1000136/150‑1 (MQ=255)

           gtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGa                                                                                                                              >  1:4255177/1‑150 (MQ=255)

           gtCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGa                                                                                                                              <  1:1670601/150‑1 (MQ=255)

             cATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCCAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATa                                                                                                                            <  1:2694431/150‑1 (MQ=255)

               ttATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAga                                                                                                                          <  1:4241867/150‑1 (MQ=255)

                tatataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaa                                                                                                                         >  1:1797317/1‑150 (MQ=255)

                  tataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaat                                                                                                                       <  2:2932939/150‑1 (MQ=255)

                   ataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatg                                                                                                                      <  2:4052442/150‑1 (MQ=255)

                   ataCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatg                                                                                                                      <  1:3573176/150‑1 (MQ=255)

                    taCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatga                                                                                                                     >  2:1701821/1‑150 (MQ=255)

                    taCTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatga                                                                                                                     >  2:1701790/1‑150 (MQ=255)

                      cTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaa                                                                                                                   <  1:2482706/150‑1 (MQ=255)

                      cTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaa                                                                                                                   <  2:4705604/150‑1 (MQ=255)

                       ttttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaat                                                                                                                  >  2:914093/1‑150 (MQ=255)

                       ttttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaat                                                                                                                  >  2:2971650/1‑150 (MQ=255)

                       ttttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaat                                                                                                                  >  2:2971668/1‑150 (MQ=255)

                       ttttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaat                                                                                                                  >  2:914072/1‑150 (MQ=255)

                       ttttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaat                                                                                                                  <  1:1597015/150‑1 (MQ=255)

                       ttttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAgaaatgaaat                                                                                                                  >  2:914081/1‑150 (MQ=255)

                        tttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaata                                                                                                                 >  1:607929/1‑150 (MQ=255)

                        tttCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaata                                                                                                                 >  1:3660998/1‑150 (MQ=255)

                              tAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaa                                                                                                           >  1:1611417/1‑150 (MQ=255)

                                         cAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATaatgaaatga                                                                                                >  2:2358320/1‑150 (MQ=255)

                                          aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                               >  1:1208062/1‑150 (MQ=255)

                                          aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                               >  1:1206399/1‑150 (MQ=255)

                                          aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                               <  1:1457326/150‑1 (MQ=255)

                                            ttttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatga                                                                                             >  2:1562523/1‑150 (MQ=255)

                                            ttttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatga                                                                                             >  2:1562517/1‑150 (MQ=255)

                                               tttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaat                                                                                          <  1:2503342/150‑1 (MQ=255)

                                               tttGGATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaat                                                                                          <  1:2503628/150‑1 (MQ=255)

                                                ttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatg                                                                                         >  2:25056/1‑150 (MQ=255)

                                                  gTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATCTTGTGATCAGTAGCACGTATCGAGGAGGCGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                       >  2:2191105/1‑150 (MQ=255)

                                                          tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgat                                                                               <  2:1483341/150‑1 (MQ=255)

                                                           cAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatg                                                                              <  1:1602577/150‑1 (MQ=255)

                                                                   ccTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatg                                                                      >  1:2876213/1‑150 (MQ=255)

                                                                   ccTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGAGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatg                                                                      >  1:2876209/1‑150 (MQ=255)

                                                                     tAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaa                                                                    >  1:542317/1‑150 (MQ=255)

                                                                     tAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaa                                                                    >  1:4291572/1‑150 (MQ=255)

                                                                       gCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaat                                                                  <  1:1716550/150‑1 (MQ=255)

                                                                        cTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatg                                                                 >  1:542651/1‑150 (MQ=255)

                                                                         ttCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatga                                                                <  1:1998556/150‑1 (MQ=255)

                                                                         ttCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatga                                                                <  1:3576893/150‑1 (MQ=255)

                                                                          tCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                               <  2:2044451/150‑1 (MQ=255)

                                                                            gCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatga                                                             >  2:1849437/1‑150 (MQ=255)

                                                                              tGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                           >  2:4759849/1‑150 (MQ=255)

                                                                              tGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                           >  2:4759920/1‑150 (MQ=255)

                                                                                         atTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACagag                                                <  2:494329/150‑1 (MQ=255)

                                                                                         atTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACagag                                                <  2:494556/150‑1 (MQ=255)

                                                                                         atTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACagag                                                >  2:4325619/1‑150 (MQ=255)

                                                                                           tgtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTTGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                              <  1:3237511/150‑1 (MQ=255)

                                                                                           tgtgATAAGTAGCACGTATCGATGATGAGTATCTCTAAAAATTTGACGCTGGGTTAGAATATGAAATTTATAGAAATTAAATAATGAAATAATGAAATGATTAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                              <  1:3237541/150‑1 (MQ=255)

                                                                                             tgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTc                                            >  2:4783205/1‑150 (MQ=255)

                                                                                              gATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTcc                                           <  2:3795095/150‑1 (MQ=255)

                                                                                               aTCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCa                                          <  2:2019123/150‑1 (MQ=255)

                                                                                                  aGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTg                                       >  1:3208542/1‑150 (MQ=255)

                                                                                                  aGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTg                                       >  1:3208516/1‑150 (MQ=255)

                                                                                                  aGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTg                                       <  1:3123145/150‑1 (MQ=255)

                                                                                                          gTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGa                               >  2:3036972/1‑150 (MQ=255)

                                                                                                                 ggaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTc                        <  1:2065743/150‑1 (MQ=255)

                                                                                                                  gaggagTAGCGCTACAACTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                       <  2:1489055/150‑1 (MQ=255)

                                                                                                                  gaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                       <  2:2422349/150‑1 (MQ=255)

                                                                                                                  gaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                       <  2:22489/150‑1 (MQ=255)

                                                                                                                   aggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                      >  2:3687746/1‑150 (MQ=255)

                                                                                                                   aggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                      <  1:1701790/150‑1 (MQ=255)

                                                                                                                   aggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                      <  1:1701821/150‑1 (MQ=255)

                                                                                                                   aggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                      <  1:3978055/150‑1 (MQ=255)

                                                                                                                     gagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTAc                    <  2:2049749/150‑1 (MQ=255)

                                                                                                                     gagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTAc                    <  2:2049719/150‑1 (MQ=255)

                                                                                                                      agTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACg                   <  1:3546310/150‑1 (MQ=255)

                                                                                                                         aGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACa                <  1:3490837/150‑1 (MQ=255)

                                                                                                                              tACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCtt           >  1:3903331/1‑150 (MQ=255)

                                                                                                                               aCAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTa          <  1:914093/150‑1 (MQ=255)

                                                                                                                               aCAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTa          <  1:914081/150‑1 (MQ=255)

                                                                                                                               aCAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTa          <  1:914072/150‑1 (MQ=255)

                                                                                                                                   aTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCtt      <  2:4291572/150‑1 (MQ=255)

                                                                                                                                   aTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCtt      <  2:542317/150‑1 (MQ=255)

                                                                                                                                   aTTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAAGATTGACAGCTTGTCCAGTGGGGACGGTTGGTGTCTTAGTACATTCTTagatg      >  2:669524/1‑146 (MQ=255)

                                                                                                                                       gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc  >  1:3144078/1‑150 (MQ=255)

                                                                                                                                       gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc  >  2:597156/1‑150 (MQ=255)

                                                                                                                                       gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc  >  2:597291/1‑150 (MQ=255)

                                                                                                                                       gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc  >  1:3717426/1‑150 (MQ=255)

                                                                                                                                       gACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATc  >  1:3713879/1‑150 (MQ=255)

TTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC  >  CP009273/1193728‑1194012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC  >  CP009273/1193729‑1194012

TTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAAT                                                                                                                                        <  SRR19973078.2405630/150‑1 (MQ=60)

   ATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTG                                                                                                                                     >  SRR19973078.4455448/1‑150 (MQ=60)

       AGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAAT                                                                                                                                 >  SRR19973078.1250334/1‑150 (MQ=60)

        GTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATT                                                                                                                                <  SRR19973078.359746/150‑1 (MQ=60)

          GTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGA                                                                                                                              >  SRR19973078.4279377/1‑150 (MQ=60)

          GTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGA                                                                                                                              <  SRR19973078.1680759/150‑1 (MQ=60)

            CATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCCAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATA                                                                                                                            <  SRR19973078.2709703/150‑1 (MQ=60)

              TTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGA                                                                                                                          <  SRR19973078.4266002/150‑1 (MQ=60)

               TATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAA                                                                                                                         >  SRR19973078.1808198/1‑150 (MQ=60)

                  ATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATG                                                                                                                      <  SRR19973078.3593277/150‑1 (MQ=60)

                     CTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAA                                                                                                                   <  SRR19973078.2496955/150‑1 (MQ=60)

                      TTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAAT                                                                                                                  <  SRR19973078.1606500/150‑1 (MQ=60)

                       TTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATA                                                                                                                 >  SRR19973078.611892/1‑150 (MQ=60)

                       TTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATA                                                                                                                 >  SRR19973078.3681886/1‑150 (MQ=60)

                             TAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAA                                                                                                           >  SRR19973078.1621049/1‑150 (MQ=60)

                                         AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                               >  SRR19973078.1213683/1‑150 (MQ=60)

                                         AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                               >  SRR19973078.1215372/1‑150 (MQ=60)

                                         AATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGAT                                                                                               <  SRR19973078.1466268/150‑1 (MQ=60)

                                              TTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAAT                                                                                          <  SRR19973078.2517717/150‑1 (MQ=60)

                                              TTTGGATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAAT                                                                                          <  SRR19973078.2518003/150‑1 (MQ=60)

                                                          CAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATG                                                                              <  SRR19973078.1612119/150‑1 (MQ=60)

                                                                  CCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATG                                                                      >  SRR19973078.2892525/1‑150 (MQ=60)

                                                                  CCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGAGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATG                                                                      >  SRR19973078.2892521/1‑150 (MQ=60)

                                                                    TAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAA                                                                    >  SRR19973078.545902/1‑150 (MQ=60)

                                                                    TAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAA                                                                    >  SRR19973078.4315884/1‑150 (MQ=60)

                                                                      GCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAAT                                                                  <  SRR19973078.1727017/150‑1 (MQ=60)

                                                                       CTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATG                                                                 >  SRR19973078.546237/1‑150 (MQ=60)

                                                                        TTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGA                                                                <  SRR19973078.2010144/150‑1 (MQ=60)

                                                                        TTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGA                                                                <  SRR19973078.3597005/150‑1 (MQ=60)

                                                                                          TGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTTGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTG                                              <  SRR19973078.3255740/150‑1 (MQ=60)

                                                                                          TGTGATAAGTAGCACGTATCGATGATGAGTATCTCTAAAAATTTGACGCTGGGTTAGAATATGAAATTTATAGAAATTAAATAATGAAATAATGAAATGATTAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTG                                              <  SRR19973078.3255770/150‑1 (MQ=60)

                                                                                                 AGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTG                                       >  SRR19973078.3226431/1‑150 (MQ=60)

                                                                                                 AGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTG                                       >  SRR19973078.3226457/1‑150 (MQ=60)

                                                                                                 AGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTG                                       <  SRR19973078.3140672/150‑1 (MQ=60)

                                                                                                                GGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTC                        <  SRR19973078.2077911/150‑1 (MQ=60)

                                                                                                                  AGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTT                      <  SRR19973078.1712185/150‑1 (MQ=60)

                                                                                                                  AGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTT                      <  SRR19973078.1712154/150‑1 (MQ=60)

                                                                                                                  AGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTT                      <  SRR19973078.4000509/150‑1 (MQ=60)

                                                                                                                     AGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACG                   <  SRR19973078.3566339/150‑1 (MQ=60)

                                                                                                                        AGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACA                <  SRR19973078.3510694/150‑1 (MQ=60)

                                                                                                                             TACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTT           >  SRR19973078.3925563/1‑150 (MQ=60)

                                                                                                                              ACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTA          <  SRR19973078.919773/150‑1 (MQ=60)

                                                                                                                              ACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTA          <  SRR19973078.919782/150‑1 (MQ=60)

                                                                                                                              ACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTA          <  SRR19973078.919794/150‑1 (MQ=60)

                                                                                                                                      GACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC  >  SRR19973078.3735159/1‑150 (MQ=60)

                                                                                                                                      GACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC  >  SRR19973078.3161672/1‑150 (MQ=60)

                                                                                                                                      GACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACATTAATC  >  SRR19973078.3738728/1‑150 (MQ=60)

TTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATC  >  CP009273/1193729‑1194012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`