Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,226,292 T→A 82.8% C24S (TGT→AGT)  umuD → DNA polymerase V, subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,226,2920TA82.8% 486.5 / 95.7 180C24S (TGT→AGT) umuDDNA polymerase V, subunit D
Reads supporting (aligned to +/- strand):  ref base T (20/11);  new base A (79/70);  total (99/81)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 183 total aligned reads displayed.

ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226145‑1226441
                                                                                                                                                   |                                                                                                                                                     
aTAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAgagt                                                                                                                                                     <  2:4093761/150‑1 (MQ=255)
 tAGCCTGAATCAGTATTGATCTGCTGGTAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGtgtg                                                                                                                                                    <  1:4704068/150‑1 (MQ=255)
      tGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCttt                                                                                                                                               >  2:276965/1‑150 (MQ=255)
      tGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCttt                                                                                                                                               <  2:2600534/150‑1 (MQ=255)
      tGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCttt                                                                                                                                               >  1:2993439/1‑150 (MQ=255)
       gAATCAGTATTGATCTGCTGTCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTc                                                                                                                                              <  1:3679099/150‑1 (MQ=255)
       gAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTc                                                                                                                                              >  1:4683772/1‑150 (MQ=255)
        aaTCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTcc                                                                                                                                             >  1:3955804/1‑150 (MQ=255)
         aTCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCt                                                                                                                                            >  1:3227200/1‑150 (MQ=255)
         aTCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCt                                                                                                                                            <  1:2154847/150‑1 (MQ=255)
          tCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCtt                                                                                                                                           <  1:321012/150‑1 (MQ=255)
           cAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTc                                                                                                                                          >  1:357404/1‑150 (MQ=255)
           cAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTc                                                                                                                                          <  1:2446176/150‑1 (MQ=255)
            aGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTTTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTGCttga                                                                                                                                         >  1:4363916/1‑148 (MQ=255)
             gTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAc                                                                                                                                        >  1:735351/1‑150 (MQ=255)
             gTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAc                                                                                                                                        >  1:3103610/1‑150 (MQ=255)
               aTTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGACATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCg                                                                                                                                      >  2:780410/1‑150 (MQ=255)
                     ctgctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagca                                                                                                                                <  1:4333456/150‑1 (MQ=255)
                      tgctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCAACGGcagcag                                                                                                                               >  1:1589491/1‑150 (MQ=255)
                         tgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAtt                                                                                                                            >  2:1550610/1‑150 (MQ=255)
                          ggCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTa                                                                                                                           >  2:3627910/1‑150 (MQ=255)
                          ggCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTa                                                                                                                           >  2:3629219/1‑150 (MQ=255)
                            cAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACg                                                                                                                         <  2:1739462/150‑1 (MQ=255)
                                aaCAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGa                                                                                                                     <  2:2221215/150‑1 (MQ=255)
                                aaCAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGa                                                                                                                     >  1:1517197/1‑150 (MQ=255)
                                 aCAGACTACTGTATATAAAAACAGTATAACGTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGaa                                                                                                                    <  2:4318681/150‑1 (MQ=255)
                                   aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa                                                                                                                  <  2:2171395/150‑1 (MQ=255)
                                     actactGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgc                                                                                                                <  2:4566312/150‑1 (MQ=255)
                                       tactGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgcgc                                                                                                              <  2:1297136/150‑1 (MQ=255)
                                        actGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCa                                                                                                             >  2:3229306/1‑150 (MQ=255)
                                          tGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTAAGCTATTTATCGATCTTTTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTAAGTTTAACAGCGCGTc                                                                                                           >  2:4065744/1‑150 (MQ=255)
                                            tatataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                                                         >  2:503104/1‑150 (MQ=255)
                                            tatataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                                                         <  1:2317262/150‑1 (MQ=255)
                                            tatataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                                                         <  1:2317280/150‑1 (MQ=255)
                                              tataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc                                                                                                       <  1:2430500/150‑1 (MQ=255)
                                                taAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTg                                                                                                     <  2:4177692/150‑1 (MQ=255)
                                                   aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAt                                                                                                  >  2:737161/1‑150 (MQ=255)
                                                           taACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGtt                                                                                          >  2:3156273/1‑150 (MQ=255)
                                                           taACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGtt                                                                                          <  2:3676480/150‑1 (MQ=255)
                                                            aaCTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTg                                                                                         >  2:3265857/1‑150 (MQ=255)
                                                              cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAt                                                                                       <  2:1155929/150‑1 (MQ=255)
                                                                tCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATcc                                                                                     <  1:1345767/150‑1 (MQ=255)
                                                                  aGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACATAACCGGCAGCACAAAACGTTGAACAACGCAACGAACTGAAACAACAGTTGATACac                                                                                   >  1:3182914/1‑149 (MQ=255)
                                                                   ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGc                                                                                  <  2:699921/150‑1 (MQ=255)
                                                                    gCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCa                                                                                 <  2:1174199/150‑1 (MQ=255)
                                                                     cAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAt                                                                                >  1:2573688/1‑150 (MQ=255)
                                                                      aGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATc                                                                               >  2:381443/1‑150 (MQ=255)
                                                                      aGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATc                                                                               >  2:2754308/1‑150 (MQ=255)
                                                                          tattatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTGAAAAGCGCATCGATCTGAATCAACTGTTGATACAGCATCCCAg                                                                           >  2:133323/1‑150 (MQ=255)
                                                                           attatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgc                                                                          >  2:1240458/1‑150 (MQ=255)
                                                                             tatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgcgc                                                                        >  1:271404/1‑150 (MQ=255)
                                                                                      tagcacgCCTGCGGCTCTCCGCGAAAGAGTGACTTTTCCGCTATTTAGCGATCTGGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttactt                                                               <  2:2381931/144‑1 (MQ=255)
                                                                                      tATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttactt                                                               <  1:2676301/150‑1 (MQ=255)
                                                                                          aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc                                                           >  2:2115174/1‑150 (MQ=255)
                                                                                          aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAACGATCTGAATCAACTGTTGATCAAGCAACACAGCGCGACTTACTTCGTc                                                           >  2:4077189/1‑150 (MQ=255)
                                                                                          aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTAAGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGta                                                           >  2:3592019/1‑149 (MQ=255)
                                                                                           aGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCa                                                          <  1:1134039/150‑1 (MQ=255)
                                                                                           aGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTAAATCGTCa                                                          >  2:454030/1‑150 (MQ=255)
                                                                                           aGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCACCAGATTAAGTTTAACAGCGCAAAGATAAGAATCAAAAGTTGATCCAGCATACCAGCGCGacatacatagtaa                                                          >  1:2432260/1‑143 (MQ=255)
                                                                                             ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaaa                                                        >  2:1420926/1‑150 (MQ=255)
                                                                                             ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaaa                                                        <  2:1379108/150‑1 (MQ=255)
                                                                                              cTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAAAGCGCATCGATCTGAATCAACTGTTGATACAGCATCCCAGCGCGACTTACTTCGTCAAAg                                                       >  1:3023186/1‑150 (MQ=255)
                                                                                                 cGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCaa                                                    <  1:3933996/150‑1 (MQ=255)
                                                                                                    aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                                 >  2:650051/1‑150 (MQ=255)
                                                                                                    aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                                 >  2:3020660/1‑150 (MQ=255)
                                                                                                    aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                                 <  1:276965/150‑1 (MQ=255)
                                                                                                      ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                                               <  2:3160152/150‑1 (MQ=255)
                                                                                                        ccGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGa                                             <  2:402653/150‑1 (MQ=255)
                                                                                                        ccGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGa                                             <  2:2913368/150‑1 (MQ=255)
                                                                                                          gcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt                                           <  1:1134714/150‑1 (MQ=255)
                                                                                                          gcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt                                           <  2:3868454/150‑1 (MQ=255)
                                                                                                            gAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCt                                         >  1:2333221/1‑150 (MQ=255)
                                                                                                            gAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCt                                         >  2:2147754/1‑150 (MQ=255)
                                                                                                            gAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTAAGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCt                                         >  2:182652/1‑150 (MQ=255)
                                                                                                             aaaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTa                                        <  2:664729/150‑1 (MQ=255)
                                                                                                             aaaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAACCGCAGCAGATTACGTTGAACACCGCAACGATCAGAAAAAAAAGTTGTTACAGCATCCCACCGCGACTTACTTAGTCAAAGCAAGTGGTGATTCTa                                        >  1:1260441/1‑150 (MQ=255)
                                                                                                             aaaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAATGTTTATACAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTa                                        >  1:3111795/1‑150 (MQ=255)
                                                                                                              aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAt                                       <  2:1847905/150‑1 (MQ=255)
                                                                                                              aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCATCACCGGCAGCAGATTACGATGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCACCGCGACTTACTTCGTCAAAGCCAGTGGTGATTCTAt                                       >  2:520911/1‑150 (MQ=255)
                                                                                                               aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                      >  1:827528/1‑150 (MQ=255)
                                                                                                               aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                      >  1:827704/1‑150 (MQ=255)
                                                                                                                 tgtgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgat                                    >  2:727522/1‑150 (MQ=255)
                                                                                                                 tgtgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgat                                    >  2:3551342/1‑150 (MQ=255)
                                                                                                                      cTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                               >  1:3909583/1‑150 (MQ=255)
                                                                                                                          tCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATACAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggtgg                           >  2:2542084/1‑150 (MQ=255)
                                                                                                                            cGCTAGTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGaa                         <  2:623843/150‑1 (MQ=255)
                                                                                                                             gCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAAAGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTAATTCGTCAAATCAAGTGGTGATTCTATGATTGATGGTGGAAt                        >  2:4368266/1‑150 (MQ=255)
                                                                                                                                 tttAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                    <  1:216017/150‑1 (MQ=255)
                                                                                                                                  ttAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTg                   <  2:870103/150‑1 (MQ=255)
                                                                                                                                   tAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa                  <  2:4737868/150‑1 (MQ=255)
                                                                                                                                     gCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACg                <  1:1972540/150‑1 (MQ=255)
                                                                                                                                       gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGCATTAGTGACGGt              >  2:1416367/1‑150 (MQ=255)
                                                                                                                                       gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt              <  1:542997/150‑1 (MQ=255)
                                                                                                                                       gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt              <  1:542960/150‑1 (MQ=255)
                                                                                                                                            tGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAttt         >  1:1791930/1‑150 (MQ=255)
                                                                                                                                             gTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa        <  1:290311/150‑1 (MQ=255)
                                                                                                                                               tCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAAAGATCTGAATCAACTGTTGATCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGGGATTATATGATTGATGGTGGAAATAGTGACGGTGATTTACt      >  2:967864/1‑150 (MQ=255)
                                                                                                                                               tCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTTATCCAGCATCCCAGCGCGACTTAATTCGTAAAAGCAAGTGGTGATTCTATGATTGATTGTGGAATTAGTGACGGTGATTTACt      >  2:1756404/1‑150 (MQ=255)
                                                                                                                                                cAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTg     <  2:1059671/150‑1 (MQ=255)
                                                                                                                                                cAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTg     <  1:3826680/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226145‑1226441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226143‑1226441
                                                                                                                                                     |                                                                                                                                                     
TGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGA                                                                                                                                                       >  SRR19973082.3960490/1‑150 (MQ=60)
 GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAG                                                                                                                                                      >  SRR19973082.2060123/1‑150 (MQ=60)
   TAGCCTGAATCAGTATTGATCTGCTGGTAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTG                                                                                                                                                    <  SRR19973082.4732409/150‑1 (MQ=60)
        TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               >  SRR19973082.3011908/1‑150 (MQ=60)
         GAATCAGTATTGATCTGCTGTCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTC                                                                                                                                              <  SRR19973082.3701844/150‑1 (MQ=60)
         GAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTC                                                                                                                                              >  SRR19973082.2314748/1‑150 (MQ=60)
         GAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTC                                                                                                                                              >  SRR19973082.4712030/1‑150 (MQ=60)
          AATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCC                                                                                                                                             >  SRR19973082.3979832/1‑150 (MQ=60)
           ATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCT                                                                                                                                            >  SRR19973082.3246917/1‑150 (MQ=60)
           ATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCT                                                                                                                                            >  SRR19973082.3246965/1‑150 (MQ=60)
           ATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCT                                                                                                                                            <  SRR19973082.2168533/150‑1 (MQ=60)
            TCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTT                                                                                                                                           <  SRR19973082.323234/150‑1 (MQ=60)
             CAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTC                                                                                                                                          >  SRR19973082.359744/1‑150 (MQ=60)
             CAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTC                                                                                                                                          <  SRR19973082.2461464/150‑1 (MQ=60)
             CAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTC                                                                                                                                          <  SRR19973082.2461402/150‑1 (MQ=60)
              AGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTTTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTGCTTGA                                                                                                                                         >  SRR19973082.4390153/1‑150 (MQ=60)
               GTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAC                                                                                                                                        >  SRR19973082.3122514/1‑150 (MQ=60)
               GTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAC                                                                                                                                        >  SRR19973082.740204/1‑150 (MQ=60)
                TATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACC                                                                                                                                       <  SRR19973082.3392106/150‑1 (MQ=60)
                 ATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCG                                                                                                                                      >  SRR19973082.4767666/1‑150 (MQ=60)
                       CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973082.4359572/150‑1 (MQ=60)
                       CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973082.4359650/150‑1 (MQ=60)
                        TGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCAACGGCAGCAG                                                                                                                               >  SRR19973082.1599909/1‑150 (MQ=60)
                              CAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTGCTTCACCCGCAGCAGATTAAG                                                                                                                         >  SRR19973082.1196413/1‑150 (MQ=60)
                                  AACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGA                                                                                                                     <  SRR19973082.2930354/150‑1 (MQ=60)
                                  AACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGA                                                                                                                     >  SRR19973082.1527269/1‑150 (MQ=60)
                                  AACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGA                                                                                                                     <  SRR19973082.3933987/150‑1 (MQ=60)
                                            TGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATC                                                                                                           <  SRR19973082.274180/150‑1 (MQ=60)
                                              TATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGA                                                                                                         <  SRR19973082.2331663/150‑1 (MQ=60)
                                              TATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGA                                                                                                         <  SRR19973082.4726209/150‑1 (MQ=60)
                                              TATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGA                                                                                                         <  SRR19973082.2331645/150‑1 (MQ=60)
                                              TATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGA                                                                                                         <  SRR19973082.2331656/150‑1 (MQ=60)
                                                TATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATC                                                                                                       <  SRR19973082.2445603/150‑1 (MQ=60)
                                                                  TCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCC                                                                                     <  SRR19973082.1355038/150‑1 (MQ=60)
                                                                    AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCaacacccgcaccacaataacttgaaaagcgcaaccaaaagaatcaacagttgatacac                                                                                   >  SRR19973082.3202196/1‑92 (MQ=60)
                                                                    AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAG                                                                                   <  SRR19973082.1328926/150‑1 (MQ=60)
                                                                    AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACATAACCGGCAGCACAAAACGTTGAACAACGCAACGAACTGAAACAACAGTTGATacac                                                                                   >  SRR19973082.3202183/1‑146 (MQ=60)
                                                                       CAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAT                                                                                >  SRR19973082.85066/1‑150 (MQ=60)
                                                                       CAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAT                                                                                >  SRR19973082.2589719/1‑150 (MQ=60)
                                                                          ATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTAAGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATACC                                                                             >  SRR19973082.3857329/1‑150 (MQ=60)
                                                                             ATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATACAGCATCCCAGC                                                                          >  SRR19973082.686718/1‑150 (MQ=60)
                                                                               TATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGC                                                                        >  SRR19973082.273422/1‑150 (MQ=60)
                                                                                  GTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAATGTTGATCCAGCATCCCAGCGCGAC                                                                     >  SRR19973082.1386084/1‑150 (MQ=60)
                                                                                     GTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTA                                                                  <  SRR19973082.2907664/150‑1 (MQ=60)
                                                                                        TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               <  SRR19973082.303231/150‑1 (MQ=60)
                                                                                        TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               <  SRR19973082.2692749/150‑1 (MQ=60)
                                                                                             AGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCA                                                          <  SRR19973082.1141587/150‑1 (MQ=60)
                                                                                             AGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCACCAGATTAAGTTTAACAGCGCAAAGATAAGAATCAAAAGTTGATCCAGCATACCAGCGCGACatacatagtaa                                                          >  SRR19973082.2447382/1‑139 (MQ=60)
                                                                                             AGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCCGCAGCAGATTAAGTTTAACACCCCATAGATCTGAATAAAAGGTTGATCCAGCATACCAGCGCGACataattagtaa                                                          >  SRR19973082.2447398/1‑139 (MQ=60)
                                                                                             AGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTCCTTCACCGGCCGCAGATTACGTTGTACAGCGCATAGATCTGAATAAACTGTTGTTACAGCAAACCAGCGCGACTTACTTAGTCA                                                          >  SRR19973082.2447371/1‑150 (MQ=60)
                                                                                                CTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAAAGCGCATCGATCTGAATCAACTGTTGATACAGCATCCCAGCGCGACTTACTTCGTCAAAG                                                       >  SRR19973082.3041799/1‑150 (MQ=60)
                                                                                                   CGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAA                                                    <  SRR19973082.1554164/150‑1 (MQ=60)
                                                                                                   CGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAA                                                    <  SRR19973082.3957942/150‑1 (MQ=60)
                                                                                                      ATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTG                                                 <  SRR19973082.279009/150‑1 (MQ=60)
                                                                                                        CTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT                                               <  SRR19973082.3967680/150‑1 (MQ=60)
                                                                                                            GCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATT                                           <  SRR19973082.1142265/150‑1 (MQ=60)
                                                                                                              GAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCT                                         >  SRR19973082.2347685/1‑150 (MQ=60)
                                                                                                              GAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCT                                         >  SRR19973082.2347659/1‑150 (MQ=60)
                                                                                                              GAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTacgtaacccgcagcagattaagttttaaaccgccaagatattaaaaaaaagttgatacagcaaaccaccgcgacaaacttagtcaaaacaactgggggttgt                                         >  SRR19973082.2714/1‑48 (MQ=60)
                                                                                                               AAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTA                                        <  SRR19973082.4137370/150‑1 (MQ=60)
                                                                                                               AAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAACCGCAGCAGATTACGTTGAACACCGCAACGATCAGAAAAAAAAGTTGTTACAGCATCCCACCGCGACTTACTTAGTCAAAGCAAGTGGTGATTCTA                                        >  SRR19973082.1269113/1‑150 (MQ=60)
                                                                                                               AAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAATGTTTATACAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTA                                        >  SRR19973082.3130722/1‑150 (MQ=60)
                                                                                                                 ATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATG                                      >  SRR19973082.833160/1‑150 (MQ=60)
                                                                                                                 ATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATG                                      >  SRR19973082.832983/1‑150 (MQ=60)
                                                                                                                 ATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATG                                      >  SRR19973082.832927/1‑150 (MQ=60)
                                                                                                                        CTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATG                               >  SRR19973082.3933464/1‑150 (MQ=60)
                                                                                                                                   TTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGT                    <  SRR19973082.217769/150‑1 (MQ=60)
                                                                                                                                   TTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGT                    <  SRR19973082.217687/150‑1 (MQ=60)
                                                                                                                                      AGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                 >  SRR19973082.1959933/1‑150 (MQ=60)
                                                                                                                                      AGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                 <  SRR19973082.3660813/150‑1 (MQ=60)
                                                                                                                                       GCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACG                <  SRR19973082.1986022/150‑1 (MQ=60)
                                                                                                                                       GCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACG                <  SRR19973082.1984895/150‑1 (MQ=60)
                                                                                                                                         GATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT              <  SRR19973082.546862/150‑1 (MQ=60)
                                                                                                                                         GATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT              <  SRR19973082.546877/150‑1 (MQ=60)
                                                                                                                                         GATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT              <  SRR19973082.546899/150‑1 (MQ=60)
                                                                                                                                            CTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAAAAGCGCATCGATCTGAATCAACTGTTGATCCAGCATACCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAT           >  SRR19973082.2589992/1‑150 (MQ=60)
                                                                                                                                              TGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT         >  SRR19973082.1803719/1‑150 (MQ=60)
                                                                                                                                              TGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT         >  SRR19973082.3091240/1‑150 (MQ=60)
                                                                                                                                               GTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTA        <  SRR19973082.292418/150‑1 (MQ=60)
                                                                                                                                                  CAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTG     <  SRR19973082.3850250/150‑1 (MQ=60)
                                                                                                                                                   AGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAATGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGA    <  SRR19973082.3175320/150‑1 (MQ=60)
                                                                                                                                                     AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973082.3859678/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226143‑1226441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑