Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,226,292 T→A 93.7% C24S (TGT→AGT)  umuD → DNA polymerase V, subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,226,2920TA93.7% 713.7 / 29.2 206C24S (TGT→AGT) umuDDNA polymerase V, subunit D
Reads supporting (aligned to +/- strand):  ref base T (5/8);  new base A (100/93);  total (105/101)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 214 total aligned reads displayed.

GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226144‑1226441
                                                                                                                                                    |                                                                                                                                                     
 aTAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAgagt                                                                                                                                                     >  2:2336571/1‑150 (MQ=255)
  tAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGagtg                                                                                                                                                    <  1:86629/150‑1 (MQ=255)
  tAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGagtg                                                                                                                                                    <  1:2866426/150‑1 (MQ=255)
  tAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGagtg                                                                                                                                                    <  1:2831182/150‑1 (MQ=255)
      cTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCtt                                                                                                                                                >  2:2318170/1‑150 (MQ=255)
      cTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCtt                                                                                                                                                >  1:289649/1‑150 (MQ=255)
       tGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCttt                                                                                                                                               <  1:1594570/150‑1 (MQ=255)
         aaTCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTcc                                                                                                                                             >  2:3415043/1‑150 (MQ=255)
             aGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCa                                                                                                                                         >  2:3521404/1‑150 (MQ=255)
               tATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAcc                                                                                                                                       >  1:4117628/1‑150 (MQ=255)
                 ttGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCgg                                                                                                                                     <  1:2161347/150‑1 (MQ=255)
                      ctgctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagca                                                                                                                                <  2:2922900/150‑1 (MQ=255)
                      ctgctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagca                                                                                                                                >  2:2536687/1‑150 (MQ=255)
                        tctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGa                                                                                                                              <  2:438744/149‑1 (MQ=255)
                         ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGAt                                                                                                                             <  1:4353159/150‑1 (MQ=255)
                           ggCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTa                                                                                                                           <  1:4347680/150‑1 (MQ=255)
                            gCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTAc                                                                                                                          <  2:3615590/150‑1 (MQ=255)
                                   cAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAc                                                                                                                   >  2:184788/1‑150 (MQ=255)
                                    aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGGAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCCGCAGATTAAGTTGAACa                                                                                                                  >  1:1828153/1‑150 (MQ=255)
                                    aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCCGATGACGTTGAACa                                                                                                                  >  1:4159312/1‑150 (MQ=255)
                                    aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa                                                                                                                  <  1:1651799/150‑1 (MQ=255)
                                      actactGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCCGCAGCAGATTACGTTTAAAAgc                                                                                                                >  1:4031558/1‑150 (MQ=255)
                                         actGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCa                                                                                                             >  1:3851586/1‑150 (MQ=255)
                                          ctGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                                            <  2:3304775/150‑1 (MQ=255)
                                           tGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATc                                                                                                           <  1:1121083/150‑1 (MQ=255)
                                            gTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCg                                                                                                          <  2:4016371/150‑1 (MQ=255)
                                            gTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCg                                                                                                          <  2:4016877/150‑1 (MQ=255)
                                                 taAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTg                                                                                                     >  1:3060627/1‑150 (MQ=255)
                                                  aaaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGa                                                                                                    <  1:870353/150‑1 (MQ=255)
                                                  aaaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCGTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGa                                                                                                    >  1:2038302/1‑150 (MQ=255)
                                                   aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                                   >  1:1325219/1‑150 (MQ=255)
                                                   aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTAAACGGCAGCAGATTAAGTTTAACAGCGCAACGAtattaa                                                                                                   >  1:2248280/1‑145 (MQ=255)
                                                      aCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCa                                                                                                >  2:2622796/1‑150 (MQ=255)
                                                         gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACt                                                                                             <  2:1621845/150‑1 (MQ=255)
                                                          tataACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTg                                                                                            <  2:3164380/150‑1 (MQ=255)
                                                               cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAt                                                                                       >  1:2414290/1‑150 (MQ=255)
                                                               cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAt                                                                                       <  2:266800/150‑1 (MQ=255)
                                                                 tCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATcc                                                                                     <  2:4053996/150‑1 (MQ=255)
                                                                  cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCa                                                                                    <  1:1379466/150‑1 (MQ=255)
                                                                  cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTAAGTTGAACAGAGCATCGATATGAATCAACTGTTGATACa                                                                                    >  2:3869869/1‑150 (MQ=255)
                                                                   aGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAg                                                                                   >  2:106951/1‑150 (MQ=255)
                                                                   aGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAg                                                                                   <  1:985777/150‑1 (MQ=255)
                                                                     gCAGATTATTATGTTGTTTATCAAGCCTGCGGACCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTACACCGGCAGCAGAATACGTTGAACAGCGCATCGATCAGAATCAACAGTTGATCCAGCa                                                                                 >  2:333650/1‑150 (MQ=255)
                                                                       aGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATc                                                                               >  1:3547689/1‑150 (MQ=255)
                                                                       aGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATc                                                                               >  1:474151/1‑150 (MQ=255)
                                                                        gATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCATCACCGGCAGCAGATTAAGTTGAACAGCGCATCGATCTGAATCAAATGTTGATCCAGCATcc                                                                              >  1:1650757/1‑150 (MQ=255)
                                                                         attattATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc                                                                             <  2:2558181/150‑1 (MQ=255)
                                                                            attatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAAAAGCGCATCGATCTGAATAAACTGTTGATACAGCATACCAgc                                                                          >  1:2054653/1‑150 (MQ=255)
                                                                            attatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgc                                                                          <  1:2072436/150‑1 (MQ=255)
                                                                                tgttgtTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGa                                                                      >  1:1054475/1‑150 (MQ=255)
                                                                                    gttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactta                                                                  <  2:1330348/150‑1 (MQ=255)
                                                                                     tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttac                                                                 >  1:1761008/1‑150 (MQ=255)
                                                                                     tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttac                                                                 >  1:1761339/1‑150 (MQ=255)
                                                                                      ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                                <  1:1081140/150‑1 (MQ=255)
                                                                                      ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                                <  2:2421683/150‑1 (MQ=255)
                                                                                      ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                                <  1:3326134/150‑1 (MQ=255)
                                                                                        aTCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc                                                              <  1:612571/150‑1 (MQ=255)
                                                                                        aTCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc                                                              <  1:1947611/150‑1 (MQ=255)
                                                                                          cAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGt                                                            <  1:2922803/150‑1 (MQ=255)
                                                                                           aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc                                                           <  1:2973318/150‑1 (MQ=255)
                                                                                            aGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGGTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCa                                                          >  2:2446992/1‑150 (MQ=255)
                                                                                               cTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAg                                                       >  2:2119111/1‑150 (MQ=255)
                                                                                                 gCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCa                                                     <  2:3854713/150‑1 (MQ=255)
                                                                                                   ggATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAg                                                   >  1:2048411/1‑150 (MQ=255)
                                                                                                   ggATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCGTAACCCGCAGCACATTAACTTGAACACCCCAACCAACAGAAACAACAGTTGATACAACAACCCCCCCCCACATACTTAGTCAAAACaac                                                   >  1:3637813/1‑149 (MQ=255)
                                                                                                    gATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgt                                                  <  1:529877/150‑1 (MQ=255)
                                                                                                    gATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgt                                                  >  2:2273009/1‑150 (MQ=255)
                                                                                                     aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                                 <  1:1611056/150‑1 (MQ=255)
                                                                                                      tctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtgg                                                <  1:2622796/150‑1 (MQ=255)
                                                                                                       ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTTATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggg                                               >  2:127875/1‑149 (MQ=255)
                                                                                                          cgcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAAAAGCGCATCGATCTGAATCAACTGTTGATCCATCATACCAGCGCGACTTACATAGTAAAAGCAAGTGGTGAt                                            >  1:3451417/1‑150 (MQ=255)
                                                                                                          cgcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAt                                            <  2:1730730/150‑1 (MQ=255)
                                                                                                           gcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt                                           <  2:3137194/150‑1 (MQ=255)
                                                                                                             gAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCt                                         <  1:4152634/150‑1 (MQ=255)
                                                                                                             gAAATTGTGACTTTTCCGATATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCt                                         <  2:3812399/150‑1 (MQ=255)
                                                                                                               aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGTACAGCGCATCGATCTGAATAAACTGTTGATCCAGCATCCCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGGTTCTAt                                       >  1:1223309/1‑150 (MQ=255)
                                                                                                                aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                      >  2:1365139/1‑150 (MQ=255)
                                                                                                                aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                      <  2:57822/150‑1 (MQ=255)
                                                                                                                  tgtgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTGAAAAGCGCATCGATATGAATAAACAGTTTATACAGCATCCCAGCGCGACTTACTTCGTCAAATCAAGTGGTGATTCTAtgat                                    >  1:2310773/1‑150 (MQ=255)
                                                                                                                   gtgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgatt                                   <  1:3259942/150‑1 (MQ=255)
                                                                                                                     gACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTGCTTAACCGGCACCAGATTACGTTTAAAAGCGCAAAGATCTGAATCAAAAGTTTATACAGCATCCCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGATTCTAtggttga                                 >  1:370479/1‑150 (MQ=255)
                                                                                                                           tCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggtgg                           >  2:1879885/1‑150 (MQ=255)
                                                                                                                             cGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACAGTTGATCCAGCAACCCAGCGCGACTTACTTCGTCAAAGCAAGTGGGGATTCTATGATTGATGGTGGaa                         >  1:245109/1‑150 (MQ=255)
                                                                                                                              gCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTAACCGGCAGCAGATTACGTTGAAAAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAt                        >  2:2188433/1‑150 (MQ=255)
                                                                                                                                tATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTa                      <  1:333650/150‑1 (MQ=255)
                                                                                                                                tATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATAAACTGTTGATCCAGCATCCCCGCGCGACATACTTCGTCAAAGCAAGGGGGGTTTGTATGATTGATGGTGGAATTa                      >  1:2655634/1‑150 (MQ=255)
                                                                                                                                 aTTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAACGGCAGCAGATTACGTTGTAAAGCGCATCGATCTGAATCAACTGTTTATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTTATGGTGGAAATAg                     >  1:631868/1‑150 (MQ=255)
                                                                                                                                    tAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa                  >  2:3034674/1‑150 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                 <  2:2872183/150‑1 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                 <  2:419165/150‑1 (MQ=255)
                                                                                                                                         aTCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg             >  2:1169766/1‑150 (MQ=255)
                                                                                                                                         aTCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg             <  1:3802162/150‑1 (MQ=255)
                                                                                                                                          tCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATACCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGATTATATGATTGATGGTGGAATTAGTGACGGTGa            >  1:2783192/1‑150 (MQ=255)
                                                                                                                                           cTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAt           >  2:343890/1‑150 (MQ=255)
                                                                                                                                            ttGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTATATGATTGATGGTGGAATTAGTGACGGTGAtt          >  2:2036244/1‑150 (MQ=255)
                                                                                                                                            ttGTTCAGAGTGGCTTTCCATAAACGGCAGCAGATTACGTTTAACAGCGCAACGATCAGAAAAAACAGTTTATCCAGCCACCCAGCGCGACATACATCGTCAAAGCAAGTGGTGATTATAAGATTGATGGTGGAATTAGTGACCGTGAtt          >  1:1522943/1‑150 (MQ=255)
                                                                                                                                              gTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa        <  1:2525856/150‑1 (MQ=255)
                                                                                                                                              gTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa        <  2:1488019/150‑1 (MQ=255)
                                                                                                                                                tCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACt      <  2:869403/150‑1 (MQ=255)
                                                                                                                                                    tgtgGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAtt  <  1:1379350/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226144‑1226441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226144‑1226441
                                                                                                                                                    |                                                                                                                                                     
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTG                                                                                                                                                      >  SRR19973079.2886739/1‑150 (MQ=60)
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAG                                                                                                                                                      <  SRR19973079.1017651/150‑1 (MQ=60)
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAG                                                                                                                                                      <  SRR19973079.1017583/150‑1 (MQ=60)
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAG                                                                                                                                                      >  SRR19973079.434956/1‑150 (MQ=60)
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAG                                                                                                                                                      <  SRR19973079.2533579/150‑1 (MQ=60)
  TAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTG                                                                                                                                                    <  SRR19973079.2200963/150‑1 (MQ=60)
  TAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTG                                                                                                                                                    <  SRR19973079.2880971/150‑1 (MQ=60)
  TAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTG                                                                                                                                                    <  SRR19973079.2880970/150‑1 (MQ=60)
  TAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTG                                                                                                                                                    <  SRR19973079.87423/150‑1 (MQ=60)
  TAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTG                                                                                                                                                    <  SRR19973079.2845639/150‑1 (MQ=60)
     CCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCT                                                                                                                                                 <  SRR19973079.2065573/150‑1 (MQ=60)
      CTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTT                                                                                                                                                >  SRR19973079.291407/1‑150 (MQ=60)
       TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATGTAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               <  SRR19973079.2486590/150‑1 (MQ=60)
       TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               >  SRR19973079.3545925/1‑150 (MQ=60)
       TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               <  SRR19973079.1603341/150‑1 (MQ=60)
       TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               <  SRR19973079.1603338/150‑1 (MQ=60)
          ATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCA                                                                                                                                            >  SRR19973079.1906136/1‑150 (MQ=60)
               TATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACC                                                                                                                                       >  SRR19973079.4138518/1‑150 (MQ=60)
                 TTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGG                                                                                                                                     <  SRR19973079.2172417/150‑1 (MQ=60)
                   GATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCC                                                                                                                                   >  SRR19973079.2652559/1‑150 (MQ=60)
                   GATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCA                                                                                                                                   >  SRR19973079.2294208/1‑150 (MQ=60)
                   GATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCATCACCGGCA                                                                                                                                   >  SRR19973079.2293919/1‑150 (MQ=60)
                    ATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAG                                                                                                                                  >  SRR19973079.4379346/1‑150 (MQ=60)
                    ATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAG                                                                                                                                  >  SRR19973079.4379345/1‑150 (MQ=60)
                      CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973079.263648/150‑1 (MQ=60)
                         CTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGAT                                                                                                                             <  SRR19973079.4374968/150‑1 (MQ=60)
                         CTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGAT                                                                                                                             <  SRR19973079.1944298/150‑1 (MQ=60)
                           GGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTA                                                                                                                           <  SRR19973079.4369482/150‑1 (MQ=60)
                           GGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTA                                                                                                                           <  SRR19973079.4369493/150‑1 (MQ=60)
                           GGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTA                                                                                                                           <  SRR19973079.773413/150‑1 (MQ=60)
                               AGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTT                                                                                                                       >  SRR19973079.2922335/1‑150 (MQ=60)
                                 AACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTACACCGGCAGCAGATTAAGTTGA                                                                                                                     >  SRR19973079.2789222/1‑150 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGGAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCCGCAGATTAAGTTGAACA                                                                                                                  >  SRR19973079.1837747/1‑150 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  <  SRR19973079.871627/150‑1 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCCGATGACGTTGAACA                                                                                                                  >  SRR19973079.4180429/1‑150 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  <  SRR19973079.1660781/150‑1 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  <  SRR19973079.1660158/150‑1 (MQ=60)
                                      ACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCCGATTACGTTGAACAGC                                                                                                                >  SRR19973079.4052013/1‑150 (MQ=60)
                                      ACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCCGCAGCAGATTACGTTTAAAAGC                                                                                                                >  SRR19973079.4051912/1‑150 (MQ=60)
                                         ACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCA                                                                                                             >  SRR19973079.3871230/1‑150 (MQ=60)
                                           TGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATC                                                                                                           <  SRR19973079.1127440/150‑1 (MQ=60)
                                           TGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATC                                                                                                           <  SRR19973079.1127472/150‑1 (MQ=60)
                                             TATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGA                                                                                                         <  SRR19973079.1612318/150‑1 (MQ=60)
                                               TATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATC                                                                                                       <  SRR19973079.3290374/150‑1 (MQ=60)
                                                 TAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTG                                                                                                     >  SRR19973079.3076482/1‑150 (MQ=60)
                                                  AAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGA                                                                                                    >  SRR19973079.2049560/1‑150 (MQ=60)
                                                  AAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGA                                                                                                    <  SRR19973079.875388/150‑1 (MQ=60)
                                                  AAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGA                                                                                                    <  SRR19973079.3064038/150‑1 (MQ=60)
                                                  AAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCGTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGA                                                                                                    >  SRR19973079.2049016/1‑150 (MQ=60)
                                                   AAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAA                                                                                                   >  SRR19973079.1332681/1‑150 (MQ=60)
                                                   AAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTAAACGGCAGCAGATTAAGTTTAACAGCGCAacgatattaa                                                                                                   >  SRR19973079.2259926/1‑140 (MQ=60)
                                                     AACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATC                                                                                                 >  SRR19973079.2434152/1‑150 (MQ=60)
                                                      ACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCA                                                                                                <  SRR19973079.3832150/150‑1 (MQ=60)
                                                         GTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACT                                                                                             <  SRR19973079.1974417/150‑1 (MQ=60)
                                                            TAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTT                                                                                          <  SRR19973079.3339515/150‑1 (MQ=60)
                                                             AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCCGCAGATTACGTTGTACAGCGCAacgctcagaaacaacagttg                                                                                         >  SRR19973079.1110699/1‑130 (MQ=60)
                                                               CTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAT                                                                                       >  SRR19973079.2426738/1‑150 (MQ=60)
                                                               CTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAT                                                                                       <  SRR19973079.4144945/150‑1 (MQ=60)
                                                                  CAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCA                                                                                    <  SRR19973079.1387095/150‑1 (MQ=60)
                                                                  CAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCA                                                                                    <  SRR19973079.1387071/150‑1 (MQ=60)
                                                                   AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAG                                                                                   <  SRR19973079.4175336/150‑1 (MQ=60)
                                                                   AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAG                                                                                   <  SRR19973079.991356/150‑1 (MQ=60)
                                                                    GGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTTAACAGCGCAAAGATATGAATCAACTGTTGATACAGC                                                                                  >  SRR19973079.1312925/1‑150 (MQ=60)
                                                                    GGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATACAGC                                                                                  >  SRR19973079.3151904/1‑150 (MQ=60)
                                                                       AGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATC                                                                               >  SRR19973079.477022/1‑150 (MQ=60)
                                                                       AGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATC                                                                               >  SRR19973079.3565851/1‑150 (MQ=60)
                                                                        GATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCATCACCGGCAGCAGATTAAGTTGAACAGCGCATCGATCTGAATCAAATGTTGATCCAGCATCC                                                                              >  SRR19973079.1659737/1‑150 (MQ=60)
                                                                          TTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACATCACCCGCAGCAGATTAagttgtaaaccgcaaagataagaaacaacagtttttacagcaaccca                                                                            >  SRR19973079.383320/1‑103 (MQ=60)
                                                                            ATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAAAAGCGCATCGATCTGAATAAACTGTTGATACAGCATACCAGC                                                                          >  SRR19973079.2065428/1‑150 (MQ=60)
                                                                            ATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGC                                                                          <  SRR19973079.2083272/150‑1 (MQ=60)
                                                                            ATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTTAACAGCGCAAAGATCTGAATCAAATGTTTATACAGAATCACAGC                                                                          >  SRR19973079.2064741/1‑150 (MQ=60)
                                                                                TGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGA                                                                      >  SRR19973079.1060268/1‑150 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    <  SRR19973079.91698/150‑1 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    >  SRR19973079.1788702/1‑150 (MQ=60)
                                                                                    GTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTA                                                                  <  SRR19973079.1447089/150‑1 (MQ=60)
                                                                                     TTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTAC                                                                 >  SRR19973079.1770613/1‑150 (MQ=60)
                                                                                     TTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTAC                                                                 >  SRR19973079.1770430/1‑150 (MQ=60)
                                                                                     TTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTAC                                                                 >  SRR19973079.1770282/1‑150 (MQ=60)
                                                                                     TTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTAC                                                                 >  SRR19973079.1770016/1‑150 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973079.566523/150‑1 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973079.3343673/150‑1 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973079.3343154/150‑1 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973079.1087100/150‑1 (MQ=60)
                                                                                        ATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTC                                                              <  SRR19973079.1957982/150‑1 (MQ=60)
                                                                                        ATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTC                                                              <  SRR19973079.616114/150‑1 (MQ=60)
                                                                                          CAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGT                                                            <  SRR19973079.2937684/150‑1 (MQ=60)
                                                                                           AAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTC                                                           <  SRR19973079.2988602/150‑1 (MQ=60)
                                                                                           AAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTC                                                           <  SRR19973079.2988588/150‑1 (MQ=60)
                                                                                           AAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATAAAAAGTTGATACAGCATACCAGCGCGACTTACTTCGTC                                                           >  SRR19973079.3400933/1‑150 (MQ=60)
                                                                                            AGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATACAGCATACCAGCGCGACTTACTTCGTCA                                                          >  SRR19973079.624690/1‑150 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCgtaacccgcagcacattaacttgaacaccccaaccaacagaaacaacagttgatacaacaacccccccccacatacttagtcaaaacaac                                                   >  SRR19973079.3656254/1‑60 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAG                                                   >  SRR19973079.2059155/1‑150 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATAGATCTGAATCAACTGTTGATCCAGCATACAAGAGCGACTAACTTCGTCAAAtaaag                                                   >  SRR19973079.4230104/1‑145 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCATCACCCGCAGCAGATTAAGTTGAAAAGCGCATCGAACTGAATCAACTGTTGATACAGCAAACCAGCGCGACTTAattaggaaaaccaag                                                   >  SRR19973079.3657029/1‑135 (MQ=60)
                                                                                                    GATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGT                                                  <  SRR19973079.533069/150‑1 (MQ=60)
                                                                                                    GATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGT                                                  <  SRR19973079.2418629/150‑1 (MQ=60)
                                                                                                     ATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTG                                                 <  SRR19973079.1619885/150‑1 (MQ=60)
                                                                                                      TCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGG                                                <  SRR19973079.2636338/150‑1 (MQ=60)
                                                                                                          CGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAAAAGCGCATCGATCTGAATCAACTGTTGATCCATCATACCAGCGCGACTTACATAGTAAAAGCAAGTGGTGAT                                            >  SRR19973079.3469231/1‑150 (MQ=60)
                                                                                                             GAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCT                                         <  SRR19973079.4173712/150‑1 (MQ=60)
                                                                                                              AAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCACATTACGTTGAAAAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACATACTTCGTCAAAGCAAGTGGTGATTCTA                                        >  SRR19973079.2826897/1‑150 (MQ=60)
                                                                                                               AATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGTACAGCGCATCGATCTGAATAAACTGTTGATCCAGCATCCCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGGTTCTAT                                       >  SRR19973079.1230334/1‑150 (MQ=60)
                                                                                                                 TTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGA                                     >  SRR19973079.3600964/1‑150 (MQ=60)
                                                                                                                  TGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCGGCAGCAGATTACGTTGAAAAGCGCATCGATATGAATAAACAGTTTATACAGCATCCCAGCGCGACTTACTTCGTCAAATCAAGTGGTGATTCTATGAT                                    >  SRR19973079.2322769/1‑150 (MQ=60)
                                                                                                                  TGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGGTGATCCAGCATCCCCGCGCGACTTACATAGTCAAAGCAAGTGGTGtttgtatggt                                    >  SRR19973079.1475802/1‑140 (MQ=60)
                                                                                                                   GTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATT                                   <  SRR19973079.3276400/150‑1 (MQ=60)
                                                                                                                     GACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTGCTTAACCGGCACCAGATTACGTTTAAAAGCGCAAAGATCTGAATCAAAAGTTTATACAGCATCCCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGATTCTATGGTTGA                                 >  SRR19973079.372485/1‑150 (MQ=60)
                                                                                                                       CTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAAAGCGCATCGATCAGAATCAAATGTTGATACAGCATCCCCGCGCGACTTACGTCGTCAAAGCAAGTGGTGATTAGATGTTTGATG                               >  SRR19973079.3729008/1‑150 (MQ=60)
                                                                                                                       CTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCCGCAGCAGATTAAGTTTAACAGCGCAACGATATGAATAAAATGTTGATACAGCATCCCCGCGCGACTTAaatagtaaaagcaagtggtgtttgtattattgatt                               >  SRR19973079.491095/1‑115 (MQ=60)
                                                                                                                           TCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGG                           <  SRR19973079.3990445/150‑1 (MQ=60)
                                                                                                                             CGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAA                         <  SRR19973079.2837230/150‑1 (MQ=60)
                                                                                                                             CGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACAGTTGATCCAGCAACCCAGCGCGACTTACTTCGTCAAAGCAAGTGGGGATTCTATGATTGATGGTGGAA                         >  SRR19973079.246728/1‑150 (MQ=60)
                                                                                                                              GCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAT                        >  SRR19973079.1072726/1‑150 (MQ=60)
                                                                                                                               CTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATT                       <  SRR19973079.1540743/150‑1 (MQ=60)
                                                                                                                               CTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATT                       <  SRR19973079.2167589/150‑1 (MQ=60)
                                                                                                                                TATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTA                      <  SRR19973079.335523/150‑1 (MQ=60)
                                                                                                                                TATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATAAACTGTTGATCCAGCATCCCCGCGCGACATACTTCGTCAAAGCAAGGGGGGTTTGTATGATTGATGGTGGAATTA                      >  SRR19973079.2669383/1‑150 (MQ=60)
                                                                                                                                TATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCCGCAGCACATTACGTTTAACAGCGCATCGATCTGTATCAAATGTTGATACAGCAACCCACCGCGACTTACTTCGTAAAAGCAAGTGGTGATTCTATGATTGtttgggggatta                      >  SRR19973079.2669232/1‑138 (MQ=60)
                                                                                                                                 ATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAACGGCAGCAGATTACGTTGTAAAGCGCATCGATCTGAATCAACTGTTTATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTTATGGTGGAAATAG                     >  SRR19973079.635489/1‑150 (MQ=60)
                                                                                                                                 ATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCAACGATCTGAATCAACAGTTGATACAGCAACCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAG                     >  SRR19973079.1648728/1‑150 (MQ=60)
                                                                                                                                   TTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTG                   <  SRR19973079.134198/150‑1 (MQ=60)
                                                                                                                                         ATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTG             <  SRR19973079.3821622/150‑1 (MQ=60)
                                                                                                                                          TCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATACCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGATTATATGATTGATGGTGGAATTAGTGACGGTGA            >  SRR19973079.2797489/1‑150 (MQ=60)
                                                                                                                                            TTGTTCAGAGTGGCTTTCCATAAACGGCAGCAGATTACGTTTAACAGCGCAACGATCAGAAAAAACAGTTTATCCAGCCACCCAGCGCGACATACATCGTCAAAGCAAGTGGTGATTATAAGATTGATGGTGGAATTAGTGACCGTGATT          >  SRR19973079.1531332/1‑150 (MQ=53)
                                                                                                                                              GTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTA        <  SRR19973079.2538606/150‑1 (MQ=60)
                                                                                                                                                   GAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAT   <  SRR19973079.1215961/150‑1 (MQ=60)
                                                                                                                                                    TGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973079.1386978/150‑1 (MQ=60)
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GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226144‑1226441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑