Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,226,292 T→A 56.5% C24S (TGT→AGT)  umuD → DNA polymerase V, subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,226,2920TA56.5% 94.5 / 286.2 184C24S (TGT→AGT) umuDDNA polymerase V, subunit D
Reads supporting (aligned to +/- strand):  ref base T (34/46);  new base A (57/47);  total (91/93)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 189 total aligned reads displayed.

GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAC  >  CP009273/1226144‑1226436
                                                                                                                                                    |                                                                                                                                                
gATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAgtg                                                                                                                                                 >  2:208328/1‑150 (MQ=255)
 aTAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAgagt                                                                                                                                                <  2:3722062/150‑1 (MQ=255)
  tAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGtgtg                                                                                                                                               >  2:2205917/1‑150 (MQ=255)
   aGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTgg                                                                                                                                              <  2:2592289/150‑1 (MQ=255)
     ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCt                                                                                                                                            >  1:730946/1‑150 (MQ=255)
       tGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCttt                                                                                                                                          <  1:2440786/150‑1 (MQ=255)
        gAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAATAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAATTTGTGACTTTTCCGCTTTTTAGCGATCTTGTTCAGAGTGGCTTTc                                                                                                                                         <  1:3144426/150‑1 (MQ=255)
           tCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCtt                                                                                                                                      <  2:1122775/150‑1 (MQ=255)
              gTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGACATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTACGTCAc                                                                                                                                   >  1:3280045/1‑150 (MQ=255)
                aTTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCg                                                                                                                                 >  2:2936491/1‑150 (MQ=255)
                aTTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAACCg                                                                                                                                 >  2:2936622/1‑150 (MQ=255)
                     tCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagc                                                                                                                            >  1:357966/1‑150 (MQ=255)
                     tCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagc                                                                                                                            >  1:1878812/1‑150 (MQ=255)
                         ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAt                                                                                                                        >  1:4548715/1‑150 (MQ=255)
                           ggCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTAAACGGCAGCAGATTa                                                                                                                      >  2:493547/1‑150 (MQ=255)
                               aGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGtt                                                                                                                  <  1:480273/150‑1 (MQ=255)
                               aGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGtt                                                                                                                  <  1:1342254/150‑1 (MQ=255)
                                gAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTg                                                                                                                 <  2:4766226/150‑1 (MQ=255)
                                    aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa                                                                                                             <  1:4713899/150‑1 (MQ=255)
                                    aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa                                                                                                             <  2:1790480/150‑1 (MQ=255)
                                      actactGTAGATAAAAACAGTATAACGTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgc                                                                                                           <  2:3884955/150‑1 (MQ=255)
                                       ctactGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgcg                                                                                                          >  2:2229374/1‑150 (MQ=255)
                                       ctactGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgcg                                                                                                          <  2:3252692/150‑1 (MQ=255)
                                           tGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATc                                                                                                      <  2:3436709/150‑1 (MQ=255)
                                               tataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc                                                                                                  <  1:3901332/150‑1 (MQ=255)
                                                ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                 <  2:274651/150‑1 (MQ=255)
                                                ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                 >  2:1502591/1‑150 (MQ=255)
                                                ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                 >  1:4185690/1‑150 (MQ=255)
                                                ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                 >  1:4185568/1‑150 (MQ=255)
                                                 taAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTg                                                                                                >  1:2386691/1‑150 (MQ=255)
                                                 taAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTg                                                                                                >  1:2386768/1‑150 (MQ=255)
                                                   aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                              >  1:2422207/1‑150 (MQ=255)
                                                   aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                              >  2:548678/1‑150 (MQ=255)
                                                    aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAt                                                                                             <  1:983267/150‑1 (MQ=255)
                                                     aaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATc                                                                                            <  1:2466921/150‑1 (MQ=255)
                                                        aGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAc                                                                                         >  1:329461/1‑150 (MQ=255)
                                                        aGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAc                                                                                         <  2:966118/150‑1 (MQ=255)
                                                         gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACt                                                                                        >  1:2295004/1‑150 (MQ=255)
                                                         gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACt                                                                                        >  1:4743306/1‑150 (MQ=255)
                                                            taACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGtt                                                                                     <  2:1244019/150‑1 (MQ=255)
                                                            taACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGtt                                                                                     <  2:3704451/150‑1 (MQ=255)
                                                              aCTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGa                                                                                   >  1:2661008/1‑150 (MQ=255)
                                                              aCTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGa                                                                                   >  2:4578688/1‑150 (MQ=255)
                                                               cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAt                                                                                  <  1:2604450/150‑1 (MQ=255)
                                                               cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAt                                                                                  <  2:810742/150‑1 (MQ=255)
                                                                ttCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATc                                                                                 >  1:2775412/1‑150 (MQ=255)
                                                                  cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCa                                                                               <  2:2290800/150‑1 (MQ=255)
                                                                  cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCa                                                                               <  1:3922508/150‑1 (MQ=255)
                                                                  cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCa                                                                               <  1:1463750/150‑1 (MQ=255)
                                                                    ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACATCGCATAGATATGAATCAACTTTTGATACAGc                                                                             >  2:3291944/1‑150 (MQ=255)
                                                                    ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGc                                                                             >  2:2962523/1‑150 (MQ=255)
                                                                      cAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAt                                                                           >  1:489071/1‑150 (MQ=255)
                                                                      cAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAACAAATGTTGATAcatcat                                                                           >  2:4463809/1‑150 (MQ=255)
                                                                      cAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAACGATCAGAAACAAAAGTTTATCCAGCaa                                                                           >  1:81033/1‑149 (MQ=255)
                                                                          ttattaTGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCa                                                                       <  1:4571658/150‑1 (MQ=255)
                                                                               atGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGcgcg                                                                  <  1:3714360/150‑1 (MQ=255)
                                                                                 gttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCATTCACCGGCAGCAGAATACGTTTAACACCGCATCGAACTGAATAAACTGATGATACATCATCCCAGCGCGac                                                                >  1:2298769/1‑150 (MQ=255)
                                                                                  ttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGact                                                               <  1:1565571/150‑1 (MQ=255)
                                                                                   tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCACCCCAGCGCGactt                                                              <  1:3623304/150‑1 (MQ=255)
                                                                                   tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactt                                                              <  2:3224448/150‑1 (MQ=255)
                                                                                   tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactt                                                              >  2:2283436/1‑150 (MQ=255)
                                                                                   tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTAAGTTGAACAGCGCAACGATCTGAATCAACAGTTGATCCAGCATCCCAGCGCGactt                                                              >  1:1208418/1‑150 (MQ=255)
                                                                                    gttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactta                                                             <  2:4616026/150‑1 (MQ=255)
                                                                                      ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACGGTTGATCCAGCATCCCAGCGCGacttact                                                           >  1:2654922/1‑150 (MQ=255)
                                                                                          cAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGt                                                       <  1:1778196/150‑1 (MQ=255)
                                                                                           aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc                                                      >  2:1977311/1‑150 (MQ=255)
                                                                                           aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc                                                      <  1:2556855/150‑1 (MQ=255)
                                                                                              ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTGCTTCACCCGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATAAACAGTTGATACAGCAACCCAGCGCGACATACTTAGTCaaa                                                   >  1:205806/1‑150 (MQ=255)
                                                                                              ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaaa                                                   <  1:1955402/150‑1 (MQ=255)
                                                                                              ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAACCCAGCGCGACATACTTCGTCaaa                                                   >  1:205846/1‑150 (MQ=255)
                                                                                                   ggATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAg                                              >  1:2447666/1‑150 (MQ=255)
                                                                                                   ggATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAg                                              >  1:3033162/1‑150 (MQ=255)
                                                                                                    gATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgt                                             >  2:29816/1‑150 (MQ=255)
                                                                                                     aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                            >  1:2418797/1‑150 (MQ=255)
                                                                                                       ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                                          >  1:308651/1‑150 (MQ=255)
                                                                                                        tcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACATCGTCAAAGCAAgtggtg                                         >  2:1182469/1‑150 (MQ=255)
                                                                                                         ccGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGa                                        >  2:408603/1‑150 (MQ=255)
                                                                                                          cgcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAt                                       <  1:4088874/150‑1 (MQ=255)
                                                                                                           gcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt                                      <  2:3612351/150‑1 (MQ=255)
                                                                                                           gcgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt                                      <  2:3848444/150‑1 (MQ=255)
                                                                                                            cgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGTACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTc                                     >  2:238769/1‑150 (MQ=255)
                                                                                                               aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAt                                  >  2:712784/1‑150 (MQ=255)
                                                                                                                   gtgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgatt                              <  1:4798580/150‑1 (MQ=255)
                                                                                                                    tgACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattg                             <  1:1701777/150‑1 (MQ=255)
                                                                                                                    tgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCGTCACCGGCAGCCGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGTTACAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattg                             >  1:2161844/1‑150 (MQ=255)
                                                                                                                         tttCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggt                        <  2:2517500/150‑1 (MQ=255)
                                                                                                                         tttCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggt                        <  1:1576335/150‑1 (MQ=255)
                                                                                                                         tttCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggt                        <  2:2720017/150‑1 (MQ=255)
                                                                                                                          ttCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggtg                       <  2:2163519/150‑1 (MQ=255)
                                                                                                                            ccGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGa                     <  1:2939182/150‑1 (MQ=255)
                                                                                                                                tATTTAGCGATCTTTTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTa                 <  1:3476741/150‑1 (MQ=255)
                                                                                                                                tATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTa                 <  2:3811891/150‑1 (MQ=255)
                                                                                                                                 aTTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAg                >  2:2929478/1‑150 (MQ=255)
                                                                                                                                    tAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa             <  2:1253349/150‑1 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc            >  2:4622783/1‑150 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTAGTCAAAGCAAGTGGTGATTCTATGATTTATGGTGGAATTAGTGaa            >  2:3071191/1‑149 (MQ=255)
                                                                                                                                      gCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACg           <  1:7577/150‑1 (MQ=255)
                                                                                                                                        gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt         >  2:799535/1‑150 (MQ=255)
                                                                                                                                        gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACGTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt         >  2:1691007/1‑150 (MQ=255)
                                                                                                                                             tGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAttt    >  2:872116/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAC  >  CP009273/1226144‑1226436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226144‑1226441
                                                                                                                                                    |                                                                                                                                                     
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTG                                                                                                                                                      <  SRR19973081.315183/150‑1 (MQ=60)
 ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGT                                                                                                                                                     <  SRR19973081.2058110/150‑1 (MQ=60)
     CCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCT                                                                                                                                                 >  SRR19973081.735758/1‑150 (MQ=60)
       TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               <  SRR19973081.2455814/150‑1 (MQ=60)
        GAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAATAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAATTTGTGACTTTTCCGCTTTTTAGCGATCTTGTTCAGAGTGGCTTTC                                                                                                                                              <  SRR19973081.3163632/150‑1 (MQ=60)
              GTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGACATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCgttacgtcac                                                                                                                                        >  SRR19973081.3299980/1‑140 (MQ=60)
              GTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCGTTCCTTCAC                                                                                                                                        >  SRR19973081.3300009/1‑150 (MQ=60)
                     TCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGC                                                                                                                                 >  SRR19973081.360417/1‑150 (MQ=60)
                     TCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGC                                                                                                                                 >  SRR19973081.1890911/1‑150 (MQ=60)
                     TCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGC                                                                                                                                 >  SRR19973081.1891144/1‑150 (MQ=60)
                         CTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAT                                                                                                                             >  SRR19973081.2736815/1‑150 (MQ=60)
                         CTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAT                                                                                                                             >  SRR19973081.4576291/1‑150 (MQ=60)
                           GGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTA                                                                                                                           >  SRR19973081.2177298/1‑150 (MQ=60)
                             CAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACG                                                                                                                         <  SRR19973081.1716860/150‑1 (MQ=60)
                               AGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTT                                                                                                                       <  SRR19973081.1351250/150‑1 (MQ=60)
                               AGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTT                                                                                                                       <  SRR19973081.483650/150‑1 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  >  SRR19973081.267937/1‑150 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  <  SRR19973081.4742453/150‑1 (MQ=60)
                                    AGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTACCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  <  SRR19973081.4742733/150‑1 (MQ=60)
                                     GACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAG                                                                                                                 >  SRR19973081.3502062/1‑150 (MQ=60)
                                           TGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATC                                                                                                           <  SRR19973081.2548818/150‑1 (MQ=60)
                                            GTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCG                                                                                                          >  SRR19973081.764397/1‑150 (MQ=60)
                                               TATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATC                                                                                                       <  SRR19973081.3925290/150‑1 (MQ=60)
                                               TATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACATTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATC                                                                                                       <  SRR19973081.1557455/150‑1 (MQ=60)
                                                ATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCT                                                                                                      >  SRR19973081.4211164/1‑150 (MQ=60)
                                                ATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCT                                                                                                      >  SRR19973081.4211280/1‑150 (MQ=60)
                                                ATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCT                                                                                                      >  SRR19973081.4211157/1‑150 (MQ=60)
                                                ATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTAGTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCT                                                                                                      >  SRR19973081.2467101/1‑150 (MQ=60)
                                                 TAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTG                                                                                                     >  SRR19973081.2401437/1‑150 (MQ=60)
                                                 TAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTG                                                                                                     >  SRR19973081.2401458/1‑150 (MQ=60)
                                                 TAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTG                                                                                                     >  SRR19973081.2401514/1‑150 (MQ=60)
                                                   AAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAA                                                                                                   >  SRR19973081.2437067/1‑150 (MQ=60)
                                                    AAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAT                                                                                                  <  SRR19973081.989956/150‑1 (MQ=60)
                                                    AAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAT                                                                                                  <  SRR19973081.991889/150‑1 (MQ=60)
                                                    AAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAT                                                                                                  <  SRR19973081.244098/150‑1 (MQ=60)
                                                     AACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATC                                                                                                 <  SRR19973081.2482230/150‑1 (MQ=60)
                                                     AACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATC                                                                                                 <  SRR19973081.413960/150‑1 (MQ=60)
                                                        AGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAC                                                                                              >  SRR19973081.331818/1‑150 (MQ=60)
                                                         GTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACT                                                                                             >  SRR19973081.4771996/1‑150 (MQ=60)
                                                         GTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACT                                                                                             >  SRR19973081.2309426/1‑150 (MQ=60)
                                                              ACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGA                                                                                        >  SRR19973081.2677531/1‑150 (MQ=60)
                                                               CTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAT                                                                                       <  SRR19973081.2620364/150‑1 (MQ=60)
                                                               CTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAT                                                                                       <  SRR19973081.2620684/150‑1 (MQ=60)
                                                                TTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATC                                                                                      >  SRR19973081.2792411/1‑150 (MQ=60)
                                                                  CAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCA                                                                                    <  SRR19973081.1473473/150‑1 (MQ=60)
                                                                  CAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCA                                                                                    <  SRR19973081.3946570/150‑1 (MQ=60)
                                                                   AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAG                                                                                   <  SRR19973081.2137261/150‑1 (MQ=60)
                                                                      CAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAT                                                                                >  SRR19973081.3344672/1‑150 (MQ=60)
                                                                      CAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAT                                                                                >  SRR19973081.492537/1‑150 (MQ=60)
                                                                      CAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAACGATCagaaacaaaagtttatccagcaa                                                                                >  SRR19973081.81925/1‑127 (MQ=60)
                                                                          TTATTTTGTTGTTTATCTATCGTGAGGTTCTCCGAGAAATTGTGACTTTTCCGCTATTTTGAGATCTTGTTCATTGTGGCTTTCCTTCTCCTTCAGCATATTACTTTTAACAGCGCATCGATATTAATCAACTGTTGATCCAGCATCCCA                                                                            <  SRR19973081.4599368/150‑1 (MQ=56)
                                                                          TTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCA                                                                            <  SRR19973081.4599379/150‑1 (MQ=60)
                                                                           TATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           <  SRR19973081.3242390/150‑1 (MQ=60)
                                                                              TATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGC                                                                        >  SRR19973081.4358260/1‑150 (MQ=60)
                                                                               ATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCG                                                                       <  SRR19973081.3738392/150‑1 (MQ=60)
                                                                               ATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCG                                                                       <  SRR19973081.3737013/150‑1 (MQ=60)
                                                                                 GTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGAC                                                                     >  SRR19973081.2313189/1‑150 (MQ=60)
                                                                                 GTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCATTCACCGGCAGCAGAATACGTTTAACACCGCATCGAACTGAATAAACTGATGATACATCATCCCAGCGCGAC                                                                     >  SRR19973081.2313201/1‑150 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    <  SRR19973081.1576479/150‑1 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    <  SRR19973081.1575695/150‑1 (MQ=60)
                                                                                   TGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTT                                                                   <  SRR19973081.3645881/150‑1 (MQ=60)
                                                                                   TGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCACCCCAGCGCGACTT                                                                   <  SRR19973081.3645202/150‑1 (MQ=60)
                                                                                   TGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTACTTCACCGGCAGCAGATTAAGTTGAACAGCGCAACGATCTGAATCAACAGTTGATCCAGCATCCCAGCGCGACTT                                                                   >  SRR19973081.1216222/1‑150 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACGGTTGATCCAGCATCCCAGCGCGACTTACT                                                                >  SRR19973081.2671418/1‑150 (MQ=60)
                                                                                       TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               >  SRR19973081.4652793/1‑150 (MQ=60)
                                                                                       TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               <  SRR19973081.1887439/150‑1 (MQ=60)
                                                                                          CAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGT                                                            <  SRR19973081.892672/150‑1 (MQ=60)
                                                                                          CAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGT                                                            <  SRR19973081.1789824/150‑1 (MQ=60)
                                                                                           AAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTC                                                           <  SRR19973081.2572781/150‑1 (MQ=60)
                                                                                            AGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCA                                                          >  SRR19973081.636306/1‑150 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTGCTTCACCCGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATAAACAGTTGATACAGCAACCCAGCGCGACATACTTAGTCAAA                                                        >  SRR19973081.207580/1‑150 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAA                                                        <  SRR19973081.1967755/150‑1 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAACCCAGCGCGACATACTTCGTCAAA                                                        >  SRR19973081.207620/1‑150 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCGTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACGGTTGATCCAGCAACCCAGCGCGACGTACATCGTCAAA                                                        >  SRR19973081.2652151/1‑150 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAG                                                   >  SRR19973081.2462753/1‑150 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAG                                                   >  SRR19973081.3051857/1‑150 (MQ=60)
                                                                                                     ATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTG                                                 >  SRR19973081.2433645/1‑150 (MQ=60)
                                                                                                       CTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT                                               >  SRR19973081.310932/1‑150 (MQ=60)
                                                                                                          CGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGTTTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAT                                            <  SRR19973081.4800852/150‑1 (MQ=60)
                                                                                                          CGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAT                                            <  SRR19973081.4113688/150‑1 (MQ=60)
                                                                                                          CGAGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAT                                            <  SRR19973081.4113644/150‑1 (MQ=60)
                                                                                                            CGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTC                                          <  SRR19973081.236091/150‑1 (MQ=60)
                                                                                                              AAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTGCTTAACCGGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATCAACAGTTGATCCACCATCCCAGCGCGACTTACTTCGTCAAAACAACTGGTGTTTCTA                                        >  SRR19973081.4185455/1‑150 (MQ=60)
                                                                                                                   GTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATT                                   <  SRR19973081.4827502/150‑1 (MQ=60)
                                                                                                                   GTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATT                                   <  SRR19973081.2451526/150‑1 (MQ=60)
                                                                                                                    TGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTG                                  <  SRR19973081.1712939/150‑1 (MQ=60)
                                                                                                                    TGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTG                                  >  SRR19973081.3622159/1‑150 (MQ=60)
                                                                                                                    TGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCGTCACCGGCAGCCGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGTTACAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTG                                  >  SRR19973081.2175613/1‑150 (MQ=60)
                                                                                                                       CTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATG                               <  SRR19973081.2415355/150‑1 (MQ=60)
                                                                                                                         TTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGT                             <  SRR19973081.1586510/150‑1 (MQ=60)
                                                                                                                            CCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGA                          <  SRR19973081.2957294/150‑1 (MQ=60)
                                                                                                                            CCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGA                          <  SRR19973081.2957307/150‑1 (MQ=60)
                                                                                                                                TATTTAGCGATCTTTTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTA                      <  SRR19973081.3498090/150‑1 (MQ=60)
                                                                                                                                TATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTA                      <  SRR19973081.2980603/150‑1 (MQ=60)
                                                                                                                                 ATTTAGCGATCTTGTTCAGAGTGGCGTTACTTCACCCGCAGCAGATTACGTTGAAAACCGCAAAGCTCTGAAAAAAAAGTTTATACAGCATACCAGCGCGACTTAAGTCGTCAAAGCAAGTGGTGATTGTATGATTGTTGGTGGAATTAT                     >  SRR19973081.3299188/1‑150 (MQ=36)
                                                                                                                                      GCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACG                <  SRR19973081.7687/150‑1 (MQ=60)
                                                                                                                                      GCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACG                <  SRR19973081.7688/150‑1 (MQ=60)
                                                                                                                                        GATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT              <  SRR19973081.4855655/150‑1 (MQ=60)
                                                                                                                                         ATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTG             <  SRR19973081.1613853/150‑1 (MQ=60)
                                                                                                                                               TTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATACAGCATCCCAGCGCGACATACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACTGTGATTTAA       >  SRR19973081.1389218/1‑150 (MQ=60)
                                                                                                                                                    AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973081.4490583/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226144‑1226441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑