Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,226,292 T→A 100% C24S (TGT→AGT)  umuD → DNA polymerase V, subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,226,2920TA100.0% 815.0 / ‑6.7 226C24S (TGT→AGT) umuDDNA polymerase V, subunit D
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (125/100);  total (125/101)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACT  >  CP009273/1226145‑1226437
                                                                                                                                                    |                                                                                                                                                 
  aGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTgg                                                                                                                                               <  1:1949986/150‑1 (MQ=255)
    ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCt                                                                                                                                             <  2:682518/150‑1 (MQ=255)
    ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCt                                                                                                                                             >  1:2459959/1‑150 (MQ=255)
    ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCt                                                                                                                                             >  2:4134112/1‑150 (MQ=255)
           cAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTc                                                                                                                                      >  1:863588/1‑150 (MQ=255)
            aGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCa                                                                                                                                     >  2:1477366/1‑150 (MQ=255)
              tATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAcc                                                                                                                                   <  1:2056382/150‑1 (MQ=255)
                 tGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgc                                                                                                                                >  2:1074983/1‑150 (MQ=255)
                    tCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagc                                                                                                                             >  1:3448269/1‑150 (MQ=255)
                     ctgctgGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcagca                                                                                                                            <  1:1511548/150‑1 (MQ=255)
                     ctgctgGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGATTTCCTTCACCGgcagca                                                                                                                            >  2:4165752/1‑150 (MQ=255)
                      tgctgGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGcagcag                                                                                                                           <  1:121648/150‑1 (MQ=255)
                      tgctgGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGcagcag                                                                                                                           <  1:1857246/150‑1 (MQ=255)
                            cAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACg                                                                                                                     <  1:4010780/150‑1 (MQ=255)
                             aaGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGt                                                                                                                    >  1:2828319/1‑150 (MQ=255)
                               gAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTg                                                                                                                  <  2:3687752/150‑1 (MQ=255)
                               gAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTg                                                                                                                  >  1:1349785/1‑150 (MQ=255)
                                 aCAGACTACTGTATATAAAAACAGTATAAACTTCAGGCAGAGTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGa                                                                                                                 >  1:367534/1‑150 (MQ=255)
                                 aCAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGaa                                                                                                                >  1:2089076/1‑150 (MQ=255)
                                          tGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATc                                                                                                       >  2:540498/1‑150 (MQ=255)
                                            tatataAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                                                     <  2:2555756/150‑1 (MQ=255)
                                               ataAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                  >  2:3636747/1‑150 (MQ=255)
                                               ataAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                  >  1:4146960/1‑150 (MQ=255)
                                                taAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTg                                                                                                 >  2:199525/1‑150 (MQ=255)
                                                 aaaaaCAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGa                                                                                                <  1:2623122/150‑1 (MQ=255)
                                                 aaaaaCAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGa                                                                                                >  1:2359139/1‑150 (MQ=255)
                                                   aaaCAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAt                                                                                              >  2:3814801/1‑150 (MQ=255)
                                                        gTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACt                                                                                         <  2:1155549/150‑1 (MQ=255)
                                                          at‑aACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGt                                                                                       <  2:2370270/150‑1 (MQ=255)
                                                          at‑aACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGt                                                                                       <  2:1328452/150‑1 (MQ=255)
                                                          at‑aACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGAGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGt                                                                                       >  2:3929960/1‑150 (MQ=255)
                                                               cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACGGCGCATCGATCTGAATCAACTGTTGAt                                                                                   >  2:3870766/1‑150 (MQ=255)
                                                               cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGAt                                                                                   <  2:206107/150‑1 (MQ=255)
                                                                 tCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATcc                                                                                 <  1:3903278/150‑1 (MQ=255)
                                                                 tCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATcc                                                                                 <  2:3141782/150‑1 (MQ=255)
                                                                   aGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAg                                                                               >  2:2717074/1‑150 (MQ=255)
                                                                   aGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAg                                                                               >  2:1856148/1‑150 (MQ=255)
                                                                         attattATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc                                                                         <  2:43896/150‑1 (MQ=255)
                                                                           ttttatGTTGTTTATCAAGCCTGCGGTTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATGTTGTTCATTGTTGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGAATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                                                       <  1:3095878/148‑1 (MQ=255)
                                                                           tattatGTTGTTTATCACGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                                                       <  1:3129432/150‑1 (MQ=255)
                                                                           tattatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                                                       >  1:73612/1‑150 (MQ=255)
                                                                           tattatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                                                       <  2:655208/150‑1 (MQ=255)
                                                                           tattatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                                                       <  1:151278/150‑1 (MQ=255)
                                                                           tattatGTTGTATATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                                                       >  1:1380164/1‑150 (MQ=255)
                                                                             ttatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgcg                                                                     >  1:1641447/1‑150 (MQ=255)
                                                                                tgttgtTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGa                                                                  >  2:1107975/1‑150 (MQ=255)
                                                                                  ttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGact                                                                <  2:1554448/150‑1 (MQ=255)
                                                                                  ttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGact                                                                >  1:1337990/1‑150 (MQ=255)
                                                                                     tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttac                                                             >  2:903788/1‑150 (MQ=255)
                                                                                      ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                            >  1:3784554/1‑150 (MQ=255)
                                                                                      ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                            <  1:1494983/150‑1 (MQ=255)
                                                                                       tATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttactt                                                           <  1:493238/150‑1 (MQ=255)
                                                                                        aTCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc                                                          >  2:971382/1‑150 (MQ=255)
                                                                                          cAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGt                                                        >  1:2555232/1‑150 (MQ=255)
                                                                                           aaGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc                                                       <  2:534701/150‑1 (MQ=255)
                                                                                               cTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAg                                                   <  2:3302647/150‑1 (MQ=255)
                                                                                               cTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAg                                                   >  2:2871546/1‑150 (MQ=255)
                                                                                                   ggATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAg                                               >  1:3526562/1‑150 (MQ=255)
                                                                                                      tctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtgg                                            >  2:3591212/1‑150 (MQ=255)
                                                                                                       ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                                           <  1:2852677/150‑1 (MQ=255)
                                                                                                       ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                                           <  2:3987966/150‑1 (MQ=255)
                                                                                                       ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                                           <  1:375241/150‑1 (MQ=255)
                                                                                                            cgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTc                                      >  1:568390/1‑150 (MQ=255)
                                                                                                            cgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTc                                      >  1:1289369/1‑150 (MQ=255)
                                                                                                            cgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGAGGTGATTc                                      >  2:1873048/1‑150 (MQ=255)
                                                                                                               aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAt                                   <  2:822888/150‑1 (MQ=255)
                                                                                                               aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAt                                   >  1:3336188/1‑150 (MQ=255)
                                                                                                               aaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAt                                   >  1:3335806/1‑150 (MQ=255)
                                                                                                                aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                  >  2:3982907/1‑150 (MQ=255)
                                                                                                                aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                  >  2:2235597/1‑150 (MQ=255)
                                                                                                                  tgtgACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACGGTTGATCCAGCATCCCAGCGCGACTTACGTCGTCAAAGCAAGTGGTGATTCTCtgat                                >  2:3206933/1‑150 (MQ=255)
                                                                                                                     gACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattga                             <  2:363870/150‑1 (MQ=255)
                                                                                                                     gACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattga                             <  2:718596/150‑1 (MQ=255)
                                                                                                                       cTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                           >  1:839869/1‑150 (MQ=255)
                                                                                                                       cTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                           <  2:3405147/150‑1 (MQ=255)
                                                                                                                          ttCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAACGGCAGCAGATTACGTTGAACAGCGCATAGATCAGAAACAACTTTTGATCCAGCATCCCAGCGAGACTTACTTCGTCAAAGCAAGGGGTGTATCGATGATTGAtggtt                        >  1:2884741/1‑149 (MQ=255)
                                                                                                                           tCCGCTATTTAGCGATCTTGCTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggtgg                       <  2:1925083/150‑1 (MQ=255)
                                                                                                                             cGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGaa                     <  2:4078980/150‑1 (MQ=255)
                                                                                                                              gCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAt                    <  1:157109/150‑1 (MQ=255)
                                                                                                                               cTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAtt                   >  2:2627021/1‑150 (MQ=255)
                                                                                                                               cTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAtt                   <  1:2116767/150‑1 (MQ=255)
                                                                                                                               cTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAtt                   <  1:3421/150‑1 (MQ=255)
                                                                                                                                 aTTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAg                 <  2:443807/150‑1 (MQ=255)
                                                                                                                                    tAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa              <  1:3217947/150‑1 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc             <  2:1236534/150‑1 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc             >  2:2387385/1‑150 (MQ=255)
                                                                                                                                     aGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc             >  1:3521028/1‑150 (MQ=255)
                                                                                                                                         aTCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg         >  2:2845324/1‑150 (MQ=255)
                                                                                                                                          tCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa        >  2:3699665/1‑150 (MQ=255)
                                                                                                                                          tCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa        >  1:1014123/1‑150 (MQ=255)
                                                                                                                                           cTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAt       <  1:1002687/150‑1 (MQ=255)
                                                                                                                                           cTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAACGATCTGAATCAACTGTTGATCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAt       >  1:3149184/1‑150 (MQ=255)
                                                                                                                                             tGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAttt     >  2:196358/1‑150 (MQ=255)
                                                                                                                                             tGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAttt     >  1:3845237/1‑150 (MQ=255)
                                                                                                                                             tGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAttt     <  1:3369669/150‑1 (MQ=255)
                                                                                                                                              gTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa    >  2:3720406/1‑150 (MQ=255)
                                                                                                                                              gTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACGTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa    >  2:1799468/1‑150 (MQ=255)
                                                                                                                                               ttCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAc   >  2:3458635/1‑150 (MQ=255)
                                                                                                                                                tCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGGGATTGTATGATTGATGGTGGAATTAGTGACGGTGATTTACt  >  1:462179/1‑150 (MQ=255)
                                                                                                                                                tCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACt  >  1:4081891/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACT  >  CP009273/1226145‑1226437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226145‑1226441
                                                                                                                                                    |                                                                                                                                                     
ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGT                                                                                                                                                     >  SRR19973074.3122416/1‑150 (MQ=60)
 TAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTG                                                                                                                                                    <  SRR19973074.189530/150‑1 (MQ=60)
  AGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGG                                                                                                                                                   <  SRR19973074.1953709/150‑1 (MQ=60)
    CCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCT                                                                                                                                                 >  SRR19973074.2464512/1‑150 (MQ=60)
    CCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCT                                                                                                                                                 >  SRR19973074.3826403/1‑150 (MQ=60)
    CCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGCATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCT                                                                                                                                                 >  SRR19973074.3826264/1‑150 (MQ=60)
      TGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTT                                                                                                                                               <  SRR19973074.1306379/150‑1 (MQ=60)
           CAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTC                                                                                                                                          >  SRR19973074.865256/1‑150 (MQ=60)
           CAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTC                                                                                                                                          >  SRR19973074.3126896/1‑150 (MQ=60)
            AGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑ATCTTCAGGCAGATTATTTTGTTGTTTATCAAGCCTGCGGTTCTCCGCGTAATTGTGACTTTTCCGCTTTTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCA                                                                                                                                         <  SRR19973074.1683423/150‑1 (MQ=60)
              TATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACC                                                                                                                                       >  SRR19973074.3262857/1‑150 (MQ=60)
              TATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACC                                                                                                                                       <  SRR19973074.2060246/150‑1 (MQ=60)
              TATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACC                                                                                                                                       >  SRR19973074.3262753/1‑150 (MQ=60)
                  GATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCA                                                                                                                                   >  SRR19973074.2403535/1‑150 (MQ=60)
                  GATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCA                                                                                                                                   >  SRR19973074.2403458/1‑150 (MQ=60)
                    TCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGC                                                                                                                                 >  SRR19973074.3454610/1‑150 (MQ=60)
                    TCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGC                                                                                                                                 >  SRR19973074.3454609/1‑150 (MQ=60)
                     CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973074.2113094/150‑1 (MQ=60)
                     CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973074.554159/150‑1 (MQ=60)
                     CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973074.1514402/150‑1 (MQ=60)
                     CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCA                                                                                                                                <  SRR19973074.1514667/150‑1 (MQ=60)
                      TGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAG                                                                                                                               <  SRR19973074.1860792/150‑1 (MQ=60)
                      TGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAG                                                                                                                               <  SRR19973074.121933/150‑1 (MQ=60)
                         TGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATT                                                                                                                            <  SRR19973074.375891/150‑1 (MQ=60)
                            CAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACG                                                                                                                         <  SRR19973074.4018198/150‑1 (MQ=60)
                             AAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGT                                                                                                                        <  SRR19973074.284968/150‑1 (MQ=60)
                             AAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGT                                                                                                                        >  SRR19973074.2833552/1‑150 (MQ=60)
                               GAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTG                                                                                                                      >  SRR19973074.1352297/1‑150 (MQ=60)
                                 ACAGACTACTGTATATAAAAACAGTATAAACTTCAGGCAGAGTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGA                                                                                                                     >  SRR19973074.368213/1‑150 (MQ=60)
                                 ACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAA                                                                                                                    >  SRR19973074.2092986/1‑150 (MQ=60)
                                 ACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAA                                                                                                                    <  SRR19973074.4102559/150‑1 (MQ=60)
                                  CAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAC                                                                                                                   <  SRR19973074.2937483/150‑1 (MQ=60)
                                   AGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA                                                                                                                  >  SRR19973074.2031863/1‑150 (MQ=60)
                                             ATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGAT                                                                                                        >  SRR19973074.1364747/1‑150 (MQ=60)
                                              TATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATC                                                                                                       >  SRR19973074.958363/1‑150 (MQ=60)
                                               ATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCT                                                                                                      >  SRR19973074.4154601/1‑150 (MQ=60)
                                                 AAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGA                                                                                                    <  SRR19973074.2628027/150‑1 (MQ=60)
                                                 AAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGA                                                                                                    >  SRR19973074.2363532/1‑150 (MQ=60)
                                                       AGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAC                                                                                              <  SRR19973074.2760079/150‑1 (MQ=60)
                                                          AT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGT                                                                                           <  SRR19973074.249367/150‑1 (MQ=60)
                                                           T‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTT                                                                                          <  SRR19973074.1617159/150‑1 (MQ=60)
                                                              ACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGA                                                                                        <  SRR19973074.3643371/150‑1 (MQ=60)
                                                                 TCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCC                                                                                     <  SRR19973074.3910441/150‑1 (MQ=60)
                                                                   AGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGTCAGCAGATTACGTTGAACAGCGCATCGATATGAATCAACTGTTGATCCAG                                                                                   >  SRR19973074.938853/1‑150 (MQ=60)
                                                                         ATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCC                                                                             <  SRR19973074.583844/150‑1 (MQ=60)
                                                                           TTTTATGTTGTTTATCAAGCCTGCGGTTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATGTTGTTCATTGTTGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGAATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           <  SRR19973074.3101563/150‑1 (MQ=60)
                                                                           TATTATGTTGTTTATCACGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           <  SRR19973074.3135161/150‑1 (MQ=60)
                                                                           TATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTTAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           >  SRR19973074.73805/1‑150 (MQ=60)
                                                                           TATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           <  SRR19973074.3135171/150‑1 (MQ=60)
                                                                           TATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           <  SRR19973074.151622/150‑1 (MQ=60)
                                                                           TATTATGTTGTATATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAG                                                                           >  SRR19973074.1382711/1‑150 (MQ=60)
                                                                            ATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGC                                                                          >  SRR19973074.2053531/1‑150 (MQ=60)
                                                                             TTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCG                                                                         >  SRR19973074.1644554/1‑150 (MQ=60)
                                                                             TTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCG                                                                         >  SRR19973074.1644522/1‑150 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    >  SRR19973074.1340486/1‑150 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    >  SRR19973074.1340490/1‑150 (MQ=60)
                                                                                  TTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACT                                                                    <  SRR19973074.4135382/150‑1 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                >  SRR19973074.3791494/1‑150 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973074.1497803/150‑1 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973074.1497951/150‑1 (MQ=60)
                                                                                      TTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACT                                                                <  SRR19973074.1497800/150‑1 (MQ=60)
                                                                                       TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               <  SRR19973074.494229/150‑1 (MQ=60)
                                                                                       TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               <  SRR19973074.494083/150‑1 (MQ=60)
                                                                                       TATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTT                                                               <  SRR19973074.3428887/150‑1 (MQ=60)
                                                                                         TCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCG                                                             >  SRR19973074.1436342/1‑150 (MQ=60)
                                                                                          CAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGT                                                            >  SRR19973074.2560001/1‑150 (MQ=60)
                                                                                          CAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGT                                                            >  SRR19973074.2560000/1‑150 (MQ=60)
                                                                                          CAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGT                                                            >  SRR19973074.2213001/1‑150 (MQ=60)
                                                                                           AAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTC                                                           >  SRR19973074.275125/1‑150 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAA                                                        <  SRR19973074.1350846/150‑1 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAA                                                        <  SRR19973074.3915692/150‑1 (MQ=60)
                                                                                              CCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAA                                                        <  SRR19973074.3665533/150‑1 (MQ=60)
                                                                                                TGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGC                                                      <  SRR19973074.3431336/150‑1 (MQ=60)
                                                                                                 GCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCA                                                     <  SRR19973074.532349/150‑1 (MQ=60)
                                                                                                   GGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAG                                                   >  SRR19973074.3533057/1‑150 (MQ=60)
                                                                                                     ATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTG                                                 >  SRR19973074.1160443/1‑150 (MQ=60)
                                                                                                     ATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTG                                                 >  SRR19973074.815608/1‑150 (MQ=60)
                                                                                                       CTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT                                               <  SRR19973074.2857968/150‑1 (MQ=60)
                                                                                                       CTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT                                               <  SRR19973074.375943/150‑1 (MQ=60)
                                                                                                       CTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT                                               <  SRR19973074.375991/150‑1 (MQ=60)
                                                                                                          CGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAAAGATCTTAATAAACGGTTGATCCAGCATCCCAGCCCGACATACATCGTCAAAGCACCTGGTGAT                                            >  SRR19973074.846359/1‑150 (MQ=60)
                                                                                                            CGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTC                                          >  SRR19973074.1291815/1‑150 (MQ=60)
                                                                                                            CGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTC                                          >  SRR19973074.569505/1‑150 (MQ=60)
                                                                                                             GAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCT                                         <  SRR19973074.1522559/150‑1 (MQ=60)
                                                                                                               AATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAT                                       >  SRR19973074.3341995/1‑150 (MQ=60)
                                                                                                               AATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAT                                       >  SRR19973074.3342021/1‑150 (MQ=60)
                                                                                                               AATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAT                                       >  SRR19973074.3342377/1‑150 (MQ=60)
                                                                                                                  TGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGAT                                    <  SRR19973074.4076085/150‑1 (MQ=60)
                                                                                                                       CTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATG                               >  SRR19973074.841484/1‑150 (MQ=60)
                                                                                                                          TTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAACGGCAGCAGATTACGTTGAACAGCGCATAGATCAGAAACAACTTTTGATCCAGCATCCCAGCGAGACTTACTTCGTCAAAGCAAGGGGTGTATCGATGATTGATGGTT                            >  SRR19973074.2890093/1‑150 (MQ=60)
                                                                                                                              GCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAT                        <  SRR19973074.157464/150‑1 (MQ=60)
                                                                                                                               CTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATT                       <  SRR19973074.2120742/150‑1 (MQ=60)
                                                                                                                               CTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATT                       <  SRR19973074.3426/150‑1 (MQ=60)
                                                                                                                                    TAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGA                  <  SRR19973074.2065394/150‑1 (MQ=60)
                                                                                                                                    TAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGA                  >  SRR19973074.2874852/1‑150 (MQ=60)
                                                                                                                                    TAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGA                  <  SRR19973074.3223893/150‑1 (MQ=60)
                                                                                                                                    TAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGA                  <  SRR19973074.4169819/150‑1 (MQ=60)
                                                                                                                                     AGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                 >  SRR19973074.3527507/1‑150 (MQ=60)
                                                                                                                                     AGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACGGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                 >  SRR19973074.3527510/1‑150 (MQ=60)
                                                                                                                                        GATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGTTCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT              >  SRR19973074.3215097/1‑150 (MQ=60)
                                                                                                                                         ATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTG             <  SRR19973074.3399692/150‑1 (MQ=60)
                                                                                                                                          TCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGA            >  SRR19973074.1015021/1‑150 (MQ=60)
                                                                                                                                          TCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGA            >  SRR19973074.1016007/1‑150 (MQ=60)
                                                                                                                                           CTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAT           <  SRR19973074.1004549/150‑1 (MQ=60)
                                                                                                                                           CTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAACGATCTGAATCAACTGTTGATCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAT           >  SRR19973074.3154965/1‑150 (MQ=60)
                                                                                                                                             TGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT         >  SRR19973074.3852250/1‑150 (MQ=60)
                                                                                                                                             TGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT         <  SRR19973074.3375915/150‑1 (MQ=60)
                                                                                                                                             TGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT         <  SRR19973074.1277756/150‑1 (MQ=60)
                                                                                                                                             TGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT         <  SRR19973074.3375903/150‑1 (MQ=60)
                                                                                                                                             TGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAAACCGCATCGATCTGAATCAACTGTTGATCCAGCAACACAGCGCGACTTACTTAGTCAAAGCAAGTGGTGATTCTATTATTGATGGTGGAATTAGTGACGGTGATTT         >  SRR19973074.3852689/1‑150 (MQ=60)
                                                                                                                                                TCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCCGCGCGACTTACTTCGTCAAAGCAAGTGGGGATTGTATGATTGATGGTGGAATTAGTGACGGTGATTTACT      >  SRR19973074.463087/1‑150 (MQ=60)
                                                                                                                                                TCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACT      >  SRR19973074.4089443/1‑150 (MQ=60)
                                                                                                                                                   GAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAT   <  SRR19973074.3701744/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973074.3574830/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973074.3318121/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973074.3317980/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973074.2689043/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  <  SRR19973074.2689064/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTAT‑AACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT  >  CP009273/1226145‑1226441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑