Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 868,897 G→T 27.7% D155Y (GAC→TAC)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,8970GT27.7% 595.8 / 203.4 276D155Y (GAC→TAC) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (93/106);  new base T (33/44);  total (126/150)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

ATAAATATTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGAAC  >  CP009273/868748‑869046
                                                                                                                                                     |                                                                                                                                                     
ataaataTTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATg                                                                                                                                                       <  2:3128483/150‑1 (MQ=255)
 taaataTTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGa                                                                                                                                                      >  1:207524/1‑150 (MQ=255)
  aaataTTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGCAACGACCGTGGTCGGTGGCTTCAGATTAc                                                                                                                                                     >  2:2674421/1‑150 (MQ=255)
  aaataTTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTAc                                                                                                                                                     >  1:1218206/1‑150 (MQ=255)
    atatTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGActc                                                                                                                                                   >  1:3100281/1‑150 (MQ=255)
     tattatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGActct                                                                                                                                                  >  2:2997856/1‑150 (MQ=255)
      attatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTa                                                                                                                                                 >  1:1796165/1‑150 (MQ=255)
         atCTTTTTTCGCGGAGGCCTAATTATAAAATCGTTTTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGTGTCAGGGCGGTTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTaaca                                                                                                                                              <  1:2307657/150‑1 (MQ=255)
            ttttttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAacaaca                                                                                                                                           <  2:3387476/150‑1 (MQ=255)
              ttttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGc                                                                                                                                         >  1:2373406/1‑150 (MQ=255)
                   gcgGAGGGCGAATGATAAAATCGTTGTTTATCATCAGTAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGaa                                                                                                                                    <  1:2146475/150‑1 (MQ=255)
                    cgGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAAt                                                                                                                                   >  1:5098710/1‑150 (MQ=255)
                     ggtgggCGAATGATAAAATCGTTTTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGTTTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATc                                                                                                                                  <  2:425850/147‑1 (MQ=255)
                     ggAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATc                                                                                                                                  <  2:3961378/150‑1 (MQ=255)
                     ggAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATc                                                                                                                                  >  2:2201996/1‑150 (MQ=255)
                      gAGGGCGAATTATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATcc                                                                                                                                 <  1:768034/150‑1 (MQ=255)
                       aGGGCTAATGATAAAATCGTTGTTTATCATCTGTAAAAAGTAATTGGACCGATTGTCGACGTGTCCGGGCGGGTTATTTTTGCCGATTTTAACCCATAGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCg                                                                                                                                <  1:856990/150‑1 (MQ=255)
                          gCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCTAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCa                                                                                                                             >  2:2278678/1‑150 (MQ=255)
                           cGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCAt                                                                                                                            <  2:5102815/150‑1 (MQ=255)
                            gAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATa                                                                                                                           >  1:5114994/1‑150 (MQ=255)
                            gAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATa                                                                                                                           >  2:2105185/1‑150 (MQ=255)
                            gAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATa                                                                                                                           >  1:2254522/1‑150 (MQ=255)
                            gAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATa                                                                                                                           >  1:4624120/1‑150 (MQ=255)
                                 aTAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAAtt                                                                                                                      >  2:2056056/1‑150 (MQ=255)
                                     aaTCGTTGTTTATCATCAGTAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCtt                                                                                                                  <  1:1037947/150‑1 (MQ=255)
                                      ttCGTTGTTTATCATCAGTAAAAAGTAAATGGACCGCTTGTCGACGTGTCTGGGCGGTTTATTTTTGCCTATTTTAACCCATCTACACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCttt                                                                                                                 <  1:26187/149‑1 (MQ=255)
                                      aTCGTTTTTTATAATCAGTAACAATTAAATGGACCGCTTGTCGACGTGTCCGGGGGGGTTATTTTTGCCTATTTTAACCCATCTAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCttt                                                                                                                 <  1:25821/150‑1 (MQ=255)
                                       tCGTTGTTTATAATCAGGAACAATTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTg                                                                                                                <  1:1143328/150‑1 (MQ=255)
                                       tCGTTGTTTATAATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTg                                                                                                                <  1:3719626/150‑1 (MQ=255)
                                        ggttgttTATCATCTGGACCATGTAATTGGCCGGGTTGTCGCCGCGTCTGGGGGGGTTATTTTGGCGGATTTTACCCCTTCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGa                                                                                                               <  1:2630232/149‑1 (MQ=255)
                                         gttgttTATCATCTGGAAAAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGAt                                                                                                              <  2:1962043/150‑1 (MQ=255)
                                         gttgttTATCATCAGGAACAAGTAAATGGACCGCTTGTCTACGAGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCATCTAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGAt                                                                                                              <  1:3562557/150‑1 (MQ=255)
                                          ttgttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATc                                                                                                             <  2:303863/150‑1 (MQ=255)
                                            gttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGt                                                                                                           >  2:2007185/1‑150 (MQ=255)
                                            gttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGt                                                                                                           >  2:500700/1‑150 (MQ=255)
                                             tttATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTc                                                                                                          >  2:4595031/1‑150 (MQ=255)
                                                  catcaGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATAGCTTTGATCGTCCGGGt                                                                                                     >  1:1574555/1‑150 (MQ=255)
                                                      aGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaa                                                                                                 >  1:3522368/1‑150 (MQ=255)
                                                        gAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaaaa                                                                                               >  2:1788708/1‑150 (MQ=255)
                                                        gAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaaaa                                                                                               >  2:2141191/1‑150 (MQ=255)
                                                        gAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaaaa                                                                                               >  2:28912/1‑150 (MQ=255)
                                                          aCATGTAATTGGACCGCTTGTCGACGAGTCTGGGCGGTTTATTTTTGCCGATTTTAACCCACCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATg                                                                                             <  1:4113451/150‑1 (MQ=255)
                                                           cAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGt                                                                                            >  2:5106203/1‑150 (MQ=255)
                                                           cAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGt                                                                                            >  2:1008/1‑150 (MQ=255)
                                                                aaaTGGACCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtctc                                                                                       <  1:3262537/150‑1 (MQ=255)
                                                                   gggACCGCTTGTCGACGAGTCTGGGCGGGTTATTTGTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCttt                                                                                    <  1:711453/149‑1 (MQ=255)
                                                                     gACCGCTTGTCGAGGTGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCAGCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAAGTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTa                                                                                  <  2:1585599/150‑1 (MQ=255)
                                                                     gACCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTa                                                                                  <  2:1235982/150‑1 (MQ=255)
                                                                     gACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTa                                                                                  <  2:455379/150‑1 (MQ=255)
                                                                     gACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTa                                                                                  <  2:2393055/150‑1 (MQ=255)
                                                                     gACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTa                                                                                  >  2:4004089/1‑150 (MQ=255)
                                                                     gACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTa                                                                                  <  1:2516235/150‑1 (MQ=255)
                                                                         gCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCt                                                                              <  2:1037802/150‑1 (MQ=255)
                                                                           ttGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTac                                                                            <  1:3609512/150‑1 (MQ=255)
                                                                              tCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTAcaca                                                                         <  1:302227/150‑1 (MQ=255)
                                                                                 acgtGTCTGGGCGGTTTTTTTTTGCCTATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCa                                                                      <  1:1306380/150‑1 (MQ=255)
                                                                                 acgaGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCa                                                                      <  2:71675/150‑1 (MQ=255)
                                                                                 acgaGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCa                                                                      <  1:4687451/150‑1 (MQ=255)
                                                                                  cgaGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCaa                                                                     <  1:3376318/150‑1 (MQ=255)
                                                                                   gaGTCAGGGCGGGTTATTTTTGCCGATTTAAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAAc                                                                    <  1:2756579/150‑1 (MQ=255)
                                                                                    aGTCAGGGCGGGTTATTTTTGCCGATTTAAACCCATCGACACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACg                                                                   <  1:1888848/150‑1 (MQ=255)
                                                                                     gTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACgg                                                                  >  1:4753088/1‑150 (MQ=255)
                                                                                     gTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAAATGCTTTGATCGTCCTGGAAAAAAATGTATCTCTATTACGCTACACATCAACgg                                                                  >  2:1513557/1‑150 (MQ=255)
                                                                                      tCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGc                                                                 >  2:1931720/1‑150 (MQ=255)
                                                                                        tggggggGTTTTTTTGGCCGATTTTACCCCTTCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCaa                                                               <  1:2662536/149‑1 (MQ=255)
                                                                                        aGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCaa                                                               >  2:4153172/1‑150 (MQ=255)
                                                                                        aGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCaa                                                               <  1:1383469/150‑1 (MQ=255)
                                                                                         gggCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCaaa                                                              <  1:1666579/150‑1 (MQ=255)
                                                                                                ttATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACg                                                       <  2:3544815/150‑1 (MQ=255)
                                                                                                  aTTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGAt                                                     <  2:4830103/150‑1 (MQ=255)
                                                                                                    ttttGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGAttt                                                   <  1:2820428/150‑1 (MQ=255)
                                                                                                        gCCGATTTTACCCCATCGACACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTa                                               <  1:508253/150‑1 (MQ=255)
                                                                                                         ccGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAg                                              >  1:175061/1‑150 (MQ=255)
                                                                                                            aTTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGTCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTCTCTCTTTTACTCTACACATCAACGGCACAGATCAAGATTTGTTAGCgg                                           >  1:4488939/1‑150 (MQ=255)
                                                                                                             ttttAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGt                                          >  2:4406127/1‑150 (MQ=255)
                                                                                                             ttttAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGt                                          >  1:1001905/1‑150 (MQ=255)
                                                                                                              tttAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTg                                         <  2:680636/150‑1 (MQ=255)
                                                                                                                tAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGa                                       <  2:1149558/150‑1 (MQ=255)
                                                                                                                tAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGa                                       <  2:3369720/150‑1 (MQ=255)
                                                                                                                  aCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATa                                     <  2:3446386/150‑1 (MQ=255)
                                                                                                                  aCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATa                                     <  2:535100/150‑1 (MQ=255)
                                                                                                                  aCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATa                                     <  1:3832499/150‑1 (MQ=255)
                                                                                                                   cccATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATaa                                    >  2:4487461/1‑150 (MQ=255)
                                                                                                                    ccATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATaaa                                   <  2:2762622/150‑1 (MQ=255)
                                                                                                                    ccATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATaaa                                   >  2:4728711/1‑150 (MQ=255)
                                                                                                                      aTCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAAt                                 <  1:1909491/150‑1 (MQ=255)
                                                                                                                         gAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCa                              >  2:1416788/1‑150 (MQ=255)
                                                                                                                         gAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCa                              <  2:805189/150‑1 (MQ=255)
                                                                                                                           aaCGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATg                            <  2:2254522/150‑1 (MQ=255)
                                                                                                                           aaCGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATg                            <  2:3267855/150‑1 (MQ=255)
                                                                                                                             cGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTc                          <  1:534830/150‑1 (MQ=255)
                                                                                                                             cGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTc                          >  2:3666376/1‑150 (MQ=255)
                                                                                                                                 cGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAtt                      <  2:2341454/150‑1 (MQ=255)
                                                                                                                                       cGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACt                <  1:202992/150‑1 (MQ=255)
                                                                                                                                        ggTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTg               <  2:1647528/150‑1 (MQ=255)
                                                                                                                                        ggTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTg               <  2:4564163/150‑1 (MQ=255)
                                                                                                                                          tGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGc             <  2:131287/150‑1 (MQ=255)
                                                                                                                                              ttCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGata         <  1:1638427/150‑1 (MQ=255)
                                                                                                                                                  gATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTg     <  2:3759928/150‑1 (MQ=255)
                                                                                                                                                   aTGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGa    >  1:2361171/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAAATATTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGAAC  >  CP009273/868748‑869046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A