Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 868,897 G→T 33.9% D155Y (GAC→TAC)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,8970GT33.9% 301.4 / 178.5 188D155Y (GAC→TAC) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (83/41);  new base T (35/29);  total (118/70)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

TAAATATTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGAA  >  CP009273/868749‑869045
                                                                                                                                                     |                                                                                                                                                    
taaattTTATCTTTTTTCGGGGTGGGCGAATTATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGGGGGTTATTTTTGCCTATTTT‑AACCCATCTAAACGACCGTGGTCGGTGGCTTCAGATGa                                                                                                                                                     <  2:4150638/150‑1 (MQ=255)
    tattatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCAACAGTAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTActct                                                                                                                                                 <  2:403056/150‑1 (MQ=255)
      ttatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AAACCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaa                                                                                                                                               >  2:4267239/1‑150 (MQ=255)
       tatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGTTGGCTCTaac                                                                                                                                              >  1:2840814/1‑150 (MQ=255)
       tatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTaac                                                                                                                                              >  1:4151764/1‑150 (MQ=255)
       tatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTaac                                                                                                                                              >  1:3732563/1‑150 (MQ=255)
       tatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGTCTCTaaa                                                                                                                                              >  1:2841199/1‑149 (MQ=255)
        atCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaaca                                                                                                                                             >  2:4333773/1‑150 (MQ=255)
          cTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaacaac                                                                                                                                           >  2:3775541/1‑150 (MQ=255)
           ttttttCGCGGTGCTCTAATGATAAAATCGTTTTTAATCATAAGAAAAAATTAATTGGCCCGCTTGTCTACGATTCTGGGGGGTTTATTTTTGCCTATTTA‑AACCCAAACACACGACCGTGGTCGGTGGCTTCAGATGACTCTAacaaca                                                                                                                                          <  2:557786/150‑1 (MQ=255)
            tttttCGCGGAGGGCTAATTATAAAATCTTTGTTTATCATCAGGAACAATTAAATGGACCGCTTGTCGACGAGTCTGGGGGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAg                                                                                                                                         <  2:4714568/150‑1 (MQ=255)
            tttttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAg                                                                                                                                         >  1:2569112/1‑150 (MQ=255)
             ttttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGc                                                                                                                                        >  2:611187/1‑150 (MQ=255)
             ttgtCGCGGTGGGGGAATGATAAAATGGTTGTTTATCATCAGGAACATTTAATTGGACCGCTTGTCTACGAGTCTGGGGGGGTTATTTTTGCCGTTTTT‑AACCAATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGc                                                                                                                                        <  2:3528369/147‑1 (MQ=255)
               ttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTg                                                                                                                                      >  2:1665154/1‑150 (MQ=255)
                     gAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATcc                                                                                                                                >  1:4398852/1‑150 (MQ=255)
                      aGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCg                                                                                                                               >  1:3573143/1‑150 (MQ=255)
                      aGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCg                                                                                                                               >  1:628782/1‑150 (MQ=255)
                      aGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCg                                                                                                                               >  1:1164186/1‑150 (MQ=255)
                      aGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCg                                                                                                                               >  2:236950/1‑150 (MQ=255)
                      aGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCg                                                                                                                               >  1:2438652/1‑150 (MQ=255)
                       gggCTAATTATAAAATCGTTTTTTATAATCAGTAAAAATTAAATGGACCGCTTGTCGACGAGTCTGGGGGGTTTTTTTTTGCCCATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCgg                                                                                                                              <  2:1042935/150‑1 (MQ=255)
                       gggCGATTTATAAAATGTTTGTTTATAATCTGTAAAATGTAATTGGACCGGTTGTCGACGTGTCTGGGGGGTTTATTTTTGCCGTTTTT‑ACCCCATCTAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCgg                                                                                                                              <  2:2436580/150‑1 (MQ=255)
                       gggCGAATTATAAAATCGTTTTTTATCATCTGTAACAATTAAATGGACCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCgg                                                                                                                              <  2:3757268/150‑1 (MQ=255)
                            aaTGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATAc                                                                                                                         >  2:1642802/1‑150 (MQ=255)
                            aaTGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATAc                                                                                                                         >  1:3451674/1‑150 (MQ=255)
                              tGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACaa                                                                                                                       >  1:1354749/1‑150 (MQ=255)
                                aTAAAATCGTTGTTTATAATCAGTAACATTTAAATGGACCGTTTGTCGACGTGTCTGGGGGGTTTTTTTTTGCCTATTTT‑AACCCTTCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAAtt                                                                                                                     <  1:3392131/150‑1 (MQ=255)
                                 tAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTg                                                                                                                    >  2:881230/1‑150 (MQ=255)
                                  aaaaTCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGc                                                                                                                   >  1:507451/1‑150 (MQ=255)
                                  aaaaTCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AAACCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGATGGAATCAGGCATACAATTGc                                                                                                                   >  1:2899459/1‑150 (MQ=255)
                                  aaaaTCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AAACCATAGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCAGGAATACGGCATACAAtttc                                                                                                                   >  1:2899395/1‑148 (MQ=255)
                                   aaaTCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCt                                                                                                                  >  2:1719798/1‑150 (MQ=255)
                                     aTCGTTGTTTATCATCAGTAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTGGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCttt                                                                                                                <  2:2599983/150‑1 (MQ=255)
                                      tCGTTTTTTAACATCAGTAACAATTAAAGGCACCGCTTGCCGACTCTTCTGGG‑GGTTTTTTTTTGGCATTTTTAAACCAATCCACACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTg                                                                                                               <  1:2083991/150‑1 (MQ=255)
                                         ttgttTATCATCAGTAACAAGTAAAGGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCTATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATc                                                                                                            <  2:1707805/150‑1 (MQ=255)
                                         ttgttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATc                                                                                                            >  2:2622456/1‑150 (MQ=255)
                                          tgttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTGTGATCg                                                                                                           >  2:2974503/1‑150 (MQ=255)
                                              tATCATCTGTAAAAAGTAATTGGCCCGCTTGTGGACGTGTCTGGGGGGGTTTTTTTTGCCTTTTTT‑AACCCATCGACACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCg                                                                                                       <  1:180631/150‑1 (MQ=255)
                                              tATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCg                                                                                                       >  1:2252032/1‑150 (MQ=255)
                                              tATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCg                                                                                                       >  1:563416/1‑150 (MQ=255)
                                               atcatcAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCgg                                                                                                      >  2:3074833/1‑150 (MQ=255)
                                                tcatcaGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCggg                                                                                                     >  2:318501/1‑150 (MQ=255)
                                                   tcaGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaa                                                                                                  >  1:2180171/1‑150 (MQ=255)
                                                    caGGACCAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaa                                                                                                 >  2:4510826/1‑150 (MQ=255)
                                                      tgAAAAATTAATTGGCCCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCTATTTT‑AACCCAGCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaaa                                                                                               <  2:2426747/149‑1 (MQ=255)
                                                          cAAGTAATTGGACCGCTTGTCGACGAGTCAGGGCGGTTTATTTTGGCCTATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGt                                                                                           <  1:1568789/150‑1 (MQ=255)
                                                             gTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGTTCGTCCGGGTAAAAAATGTAtc                                                                                        >  1:4047327/1‑150 (MQ=255)
                                                             gTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtc                                                                                        >  1:1156461/1‑150 (MQ=255)
                                                              tAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtct                                                                                       >  1:3139725/1‑150 (MQ=255)
                                                               aaaTGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtctc                                                                                      >  1:2191952/1‑150 (MQ=255)
                                                               aaaTGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtctc                                                                                      >  1:2372562/1‑150 (MQ=255)
                                                                attGGACCGGTTGTCGACTTGTCTGGGGGTGTTATTTTTGCCTTTTTT‑AACCAATCTAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATctct                                                                                     <  1:3380663/148‑1 (MQ=255)
                                                                  tGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCttt                                                                                   >  1:3388695/1‑150 (MQ=255)
                                                                       cGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGc                                                                              >  1:3950964/1‑150 (MQ=255)
                                                                         gttGTCGTCGTGTCTGGGCGGGTTTTTTTTGCCGATTTA‑ACCCCCTCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTa                                                                            <  1:2503448/149‑1 (MQ=255)
                                                                               gacgaGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATTTATATCTTTTAAGCTACACAta                                                                      >  1:4013087/1‑149 (MQ=255)
                                                                                 cgtgTCTGGGGGGTTTATTTTTGCATTTTTT‑AACCAATCTAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCaa                                                                    <  1:2522436/147‑1 (MQ=255)
                                                                                  gcggcgggggggTTTTTTTTTGCCTATTTA‑ACCCCATCCACACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAAc                                                                   <  1:3202693/144‑1 (MQ=255)
                                                                                     tCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGc                                                                >  2:106318/1‑150 (MQ=255)
                                                                                         ggCGGTTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAg                                                            <  2:954348/150‑1 (MQ=255)
                                                                                          ggggtttATTTTTGCCTATTTT‑ACCCCATCCAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGa                                                           <  1:1697911/145‑1 (MQ=255)
                                                                                          gCGGGTTATTTTTGCCTATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGa                                                           <  2:4298417/150‑1 (MQ=255)
                                                                                          gCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGa                                                           <  2:725120/150‑1 (MQ=255)
                                                                                            gggTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATc                                                         >  1:2126868/1‑150 (MQ=255)
                                                                                              gTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCAc                                                       >  2:3360782/1‑150 (MQ=255)
                                                                                                 aTTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGAt                                                    >  1:2262797/1‑150 (MQ=255)
                                                                                                    tttGCCTTTTTT‑AACCCATCGAAACGACCGTGGTAGGTGGCTTAAGATTACTCTAACAACCTCTGGAATACGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTg                                                 <  1:4194548/150‑1 (MQ=255)
                                                                                                    tttGCCGATTTT‑AACCAATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTg                                                 <  1:2748742/150‑1 (MQ=255)
                                                                                                     ttGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGt                                                >  1:566623/1‑150 (MQ=255)
                                                                                                           ttttt‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCgg                                          <  2:3250724/149‑1 (MQ=255)
                                                                                                              tt‑AACCCATAGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTgg                                       >  2:564348/1‑150 (MQ=255)
                                                                                                                 aaCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGAt                                     >  2:3646417/1‑150 (MQ=255)
                                                                                                                    ccATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATaaa                                  >  1:2791353/1‑150 (MQ=255)
                                                                                                                        cGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTc                              >  2:4125909/1‑150 (MQ=255)
                                                                                                                         taaaCGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCa                             <  2:4685709/149‑1 (MQ=255)
                                                                                                                         gAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCa                             >  2:1382324/1‑150 (MQ=255)
                                                                                                                         gAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCa                             <  2:3507596/150‑1 (MQ=255)
                                                                                                                          aaaCGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCAt                            <  2:2898770/150‑1 (MQ=255)
                                                                                                                             cGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTc                         <  1:1879615/150‑1 (MQ=255)
                                                                                                                             cGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTc                         <  1:1879741/150‑1 (MQ=255)
                                                                                                                             cGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTc                         >  1:1389187/1‑150 (MQ=255)
                                                                                                                              gACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCg                        >  1:1498957/1‑150 (MQ=255)
                                                                                                                              gACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCg                        >  1:2643795/1‑150 (MQ=255)
                                                                                                                                ccGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAt                      >  2:1997706/1‑150 (MQ=255)
                                                                                                                                  gTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAttt                    <  2:4183988/150‑1 (MQ=255)
                                                                                                                                  gTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAttt                    <  2:2219684/150‑1 (MQ=255)
                                                                                                                                    ggTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTaa                  >  2:2277396/1‑150 (MQ=255)
                                                                                                                                      tCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAAc                <  2:3573143/150‑1 (MQ=255)
                                                                                                                                      tCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAAc                <  2:1164186/150‑1 (MQ=255)
                                                                                                                                       cGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACt               <  2:132185/150‑1 (MQ=255)
                                                                                                                                         gTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTgg             >  2:488758/1‑150 (MQ=255)
                                                                                                                                          tGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGc            >  2:2093904/1‑150 (MQ=255)
                                                                                                                                             cTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGat         >  2:1816866/1‑150 (MQ=255)
                                                                                                                                               tCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGatat       >  1:923705/1‑150 (MQ=255)
                                                                                                                                                cAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATc      <  1:3547988/150‑1 (MQ=255)
                                                                                                                                                cAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATc      <  1:1138458/150‑1 (MQ=255)
                                                                                                                                                  gATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTg    <  1:2467932/150‑1 (MQ=255)
                                                                                                                                                    tGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGaa  <  1:2314843/150‑1 (MQ=255)
                                                                                                                                                    tGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGaa  >  2:856817/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAATATTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTT‑AACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTGAA  >  CP009273/868749‑869045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A