Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 868,897 G→T 37.4% D155Y (GAC→TAC)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,8970GT37.4% 287.9 / 206.3 198D155Y (GAC→TAC) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (60/64);  new base T (32/42);  total (92/106)
Fisher's exact test for biased strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 199 total aligned reads displayed.

ATAAATATTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTG  >  CP009273/868748‑869043
                                                                                                                                                     |                                                                                                                                                  
   aataTTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGAct                                                                                                                                                 >  2:1379864/1‑150 (MQ=255)
       ttatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTaa                                                                                                                                             >  2:842723/1‑150 (MQ=255)
        tatCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaac                                                                                                                                            >  2:1238834/1‑150 (MQ=255)
          tCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTaacaa                                                                                                                                          >  1:2267870/1‑150 (MQ=255)
           cTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaacaac                                                                                                                                         >  1:1069441/1‑150 (MQ=255)
           cTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaacaac                                                                                                                                         >  1:2729465/1‑150 (MQ=255)
           cTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTaacaac                                                                                                                                         >  1:2729404/1‑150 (MQ=255)
             tttttCGCGGAGGGCGAATGATAAAATCTTTTTTTATAATCAGGAACAAGTAAATGGACCGCTGGTCGACGAGTCAGGGCGGTTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAg                                                                                                                                       <  2:2513375/150‑1 (MQ=255)
               tttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCt                                                                                                                                     >  2:3719530/1‑150 (MQ=255)
               tttCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCt                                                                                                                                     >  2:3158156/1‑150 (MQ=255)
                ttCGCGGCGGGCTAATTATAAAATCGTTGTTTATCATCAGGAACAATTAAATGGACCTTTTGTCTACTTGTCTGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTg                                                                                                                                    <  1:978210/150‑1 (MQ=255)
                    cggcggGCTATTTATAAAATCGTTGTTTATCATCAGTAACAATTAATTGGACCGCTTGTCGACGTGTCTGGGGGGTTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAAt                                                                                                                                <  1:3261108/150‑1 (MQ=255)
                    cgGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGTGTCAGGGCGGGTTATTTTTGCCGATTTTAACCAATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAAt                                                                                                                                <  2:2849774/150‑1 (MQ=255)
                    cgGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAAt                                                                                                                                >  1:2572729/1‑150 (MQ=255)
                     ggAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATc                                                                                                                               >  1:1431914/1‑150 (MQ=255)
                        gggCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCgg                                                                                                                            >  2:1030864/1‑150 (MQ=255)
                          gCGAATGATAAATTCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCa                                                                                                                          <  2:954248/150‑1 (MQ=255)
                            gAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCGAACAACAGCTGGAATCCGGCATa                                                                                                                        >  1:3678012/1‑150 (MQ=255)
                             aaTGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATAc                                                                                                                       >  2:2147180/1‑150 (MQ=255)
                              aTGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACa                                                                                                                      >  2:2313989/1‑150 (MQ=255)
                                taTAAAATCGTTTTTTATCATCAGTAACAATTAAATGGCCCGCTTGTCGACGAGTCTGGGCGGGTTATTTTTGCCTATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAAt                                                                                                                    <  1:3190681/149‑1 (MQ=255)
                                gATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAAt                                                                                                                    >  1:3358725/1‑150 (MQ=255)
                                gATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAAt                                                                                                                    <  2:3433418/150‑1 (MQ=255)
                                gATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAAt                                                                                                                    >  2:541058/1‑150 (MQ=255)
                                  tAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTg                                                                                                                  >  1:1384556/1‑150 (MQ=255)
                                       tCGTTGTTAATCATCTGGAACAAGTAAATGGACCGCTTGACGACGAGTCTGGGGGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTg                                                                                                             <  1:2168026/150‑1 (MQ=255)
                                         gttgttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGAt                                                                                                           >  2:41025/1‑150 (MQ=255)
                                           tgttTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCg                                                                                                         <  2:465117/150‑1 (MQ=255)
                                               tATCATCAGTAACATGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCg                                                                                                     <  1:2339193/150‑1 (MQ=255)
                                               tATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCg                                                                                                     >  1:652976/1‑150 (MQ=255)
                                                   atcaGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGACATACAATTGCTTTGATCGTCCGGGTa                                                                                                 >  2:3683263/1‑150 (MQ=255)
                                                     caGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaa                                                                                               >  1:328601/1‑150 (MQ=255)
                                                     caGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaa                                                                                               >  2:2469502/1‑150 (MQ=255)
                                                     caGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaa                                                                                               >  2:868847/1‑150 (MQ=255)
                                                       ggAACACGTAATTGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaaa                                                                                             <  2:3229797/150‑1 (MQ=255)
                                                        gAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTaaaaaa                                                                                            <  2:2958304/150‑1 (MQ=255)
                                                               tAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtct                                                                                     >  2:368260/1‑150 (MQ=255)
                                                               tAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtct                                                                                     <  2:2766347/150‑1 (MQ=255)
                                                                aaaTGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAAAAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTAtctc                                                                                    >  2:311433/1‑150 (MQ=255)
                                                                 aaTGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATctct                                                                                   <  2:1713109/150‑1 (MQ=255)
                                                                    ggACCGCTTGTCGACGAGTCTGGGCGGTTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCtttt                                                                                <  1:3201689/150‑1 (MQ=255)
                                                                    ggACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCtttt                                                                                >  2:3324877/1‑150 (MQ=255)
                                                                         gCTTGTCGACGAGTCAGGGCGGTTTATTTTTGCCTATTTTACCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCt                                                                           <  1:1463017/150‑1 (MQ=255)
                                                                                gacgaGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATc                                                                    <  2:42146/150‑1 (MQ=255)
                                                                                  cgaGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCaa                                                                  >  1:2090417/1‑150 (MQ=255)
                                                                                        aGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCaa                                                            >  1:3113623/1‑150 (MQ=255)
                                                                                           gCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGa                                                         <  2:2240964/150‑1 (MQ=255)
                                                                                           gCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGa                                                         <  2:581392/150‑1 (MQ=255)
                                                                                            cGGGTTATTTTTGCCGATTTTAACCAATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACTCTACACATCAACGGAAAAGAt                                                        >  1:1192697/1‑150 (MQ=255)
                                                                                              ggTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCa                                                      >  2:1514803/1‑150 (MQ=255)
                                                                                              ggTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCa                                                      >  2:3377935/1‑150 (MQ=255)
                                                                                               tttATTTTTGCCTATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCAc                                                     <  1:1303340/149‑1 (MQ=255)
                                                                                               gTTATTTTTGCCGATTTTAACCCATCTAAACGCCCGTGGTCGGTGGCTTCAGATGACTCTAAAAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCAc                                                     <  1:2494083/150‑1 (MQ=255)
                                                                                                  aTTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGAt                                                  <  1:4105551/150‑1 (MQ=255)
                                                                                                  aTTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGAt                                                  <  2:3652191/150‑1 (MQ=255)
                                                                                                      ttGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGt                                              <  1:2392932/150‑1 (MQ=255)
                                                                                                      ttGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGt                                              <  1:2393002/150‑1 (MQ=255)
                                                                                                      ttGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGt                                              <  1:1018942/150‑1 (MQ=255)
                                                                                                       tGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGtt                                             <  2:1455454/150‑1 (MQ=255)
                                                                                                        gCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTa                                            >  1:41325/1‑150 (MQ=255)
                                                                                                        gCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTa                                            <  2:1580461/150‑1 (MQ=255)
                                                                                                         ccGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAg                                           >  1:1716831/1‑150 (MQ=255)
                                                                                                          cGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGc                                          >  2:1925637/1‑150 (MQ=255)
                                                                                                           gATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCg                                         >  2:3515792/1‑150 (MQ=255)
                                                                                                            aTTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCgg                                        >  2:462729/1‑150 (MQ=255)
                                                                                                                taccccATCTAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGa                                    <  1:2208742/147‑1 (MQ=255)
                                                                                                                tAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGa                                    <  1:907073/150‑1 (MQ=255)
                                                                                                                tAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGa                                    <  1:1713457/150‑1 (MQ=255)
                                                                                                                 aaCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGGGGAt                                   >  2:819765/1‑150 (MQ=255)
                                                                                                                  aCCCATCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACCGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATa                                  <  2:670875/150‑1 (MQ=255)
                                                                                                                      aTCGAAACGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAAt                              <  1:3829155/150‑1 (MQ=255)
                                                                                                                      aTCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAAt                              <  2:2403049/150‑1 (MQ=255)
                                                                                                                         gAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCa                           >  1:2747758/1‑150 (MQ=255)
                                                                                                                          aaaCGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCAt                          <  2:2718218/150‑1 (MQ=255)
                                                                                                                          aaaCGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCAt                          >  1:3513468/1‑150 (MQ=255)
                                                                                                                           aaCGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATg                         <  1:2975827/150‑1 (MQ=255)
                                                                                                                            aCGACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGt                        <  2:2643126/150‑1 (MQ=255)
                                                                                                                            aCGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGt                        <  1:2775606/150‑1 (MQ=255)
                                                                                                                              gACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCg                      <  2:2557006/150‑1 (MQ=255)
                                                                                                                              gACCGTGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCg                      <  1:1594711/150‑1 (MQ=255)
                                                                                                                              gACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAATTTCATGTCg                      <  1:3622856/150‑1 (MQ=255)
                                                                                                                                 cGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAtt                   <  1:4158178/150‑1 (MQ=255)
                                                                                                                                 cGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAtt                   <  1:807214/150‑1 (MQ=255)
                                                                                                                                 cGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAtt                   <  2:3605616/150‑1 (MQ=255)
                                                                                                                                  gTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAATTTCATGTCGAttt                  >  1:190148/1‑150 (MQ=255)
                                                                                                                                   tGGTCGGTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTa                 >  2:4123828/1‑150 (MQ=255)
                                                                                                                                    ggTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTaa                <  1:774634/150‑1 (MQ=255)
                                                                                                                                     gTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTaaa               <  2:3939880/150‑1 (MQ=255)
                                                                                                                                      tCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGAttttatc              >  2:2542953/1‑146 (MQ=255)
                                                                                                                                         gTGGCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTgg           <  1:3726517/150‑1 (MQ=255)
                                                                                                                                           ggCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAATTTCATGTCGATTTAAACTGGCg         <  1:1153100/150‑1 (MQ=255)
                                                                                                                                            gCTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGa        <  2:2007473/150‑1 (MQ=255)
                                                                                                                                             cTTCAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGat       <  1:3802958/150‑1 (MQ=255)
                                                                                                                                             cTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGat       <  1:3231647/150‑1 (MQ=255)
                                                                                                                                              ttCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGata      >  1:182601/1‑150 (MQ=255)
                                                                                                                                               tCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACTAAATGTTAGCGGTGGATAAAAGTCATGTCGATAAAAACTGGCGatat     >  2:397766/1‑150 (MQ=255)
                                                                                                                                                cAGATTACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATc    <  2:3037436/150‑1 (MQ=255)
                                                                                                                                                 aGATGACTCTAACACCAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCt   <  1:12808/150‑1 (MQ=255)
                                                                                                                                                 aGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCt   <  1:12831/150‑1 (MQ=255)
                                                                                                                                                  gATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTg  >  1:3652496/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATAAATATTATCTTTTTTCGCGGAGGGCGAATGATAAAATCGTTGTTTATCATCAGGAACAAGTAAATGGACCGCTTGTCGACGAGTCAGGGCGGGTTATTTTTGCCGATTTTAACCCATCGAAACGACCGTGGTCGGTGGCTTCAGATGACTCTAACAACAGCTGGAATCCGGCATACAATTGCTTTGATCGTCCGGGTAAAAAATGTATCTCTTTTACGCTACACATCAACGGCAAAGATCACGATTTGTTAGCGGTGGATAAAATTCATGTCGATTTAAACTGGCGATATCTG  >  CP009273/868748‑869043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A