Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 800,488 G→T 83.8% V510V (GTG→GTT)  ybhJ → putative hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273800,4880GT83.8% 566.5 / 121.1 240V510V (GTG→GTT) ybhJputative hydratase
Reads supporting (aligned to +/- strand):  ref base G (14/25);  new base T (103/97);  total (118/122)
Fisher's exact test for biased strand distribution p-value = 8.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

Only 100 of 241 total aligned reads displayed.

GGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCGG  >  CP009273/800341‑800634
                                                                                                                                                   |                                                                                                                                                  
ggCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTaa                                                                                                                                                  <  1:3842606/150‑1 (MQ=255)
ggCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTaa                                                                                                                                                  <  1:4327135/150‑1 (MQ=255)
ggCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTaa                                                                                                                                                  >  2:1513075/1‑150 (MQ=255)
     tGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAgggg                                                                                                                                             <  1:189843/150‑1 (MQ=255)
          gACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACt                                                                                                                                        >  1:4190224/1‑150 (MQ=255)
            cGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCa                                                                                                                                      <  1:4276979/150‑1 (MQ=255)
                 gTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAAc                                                                                                                                 >  1:2440929/1‑150 (MQ=255)
                  ttCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAAcc                                                                                                                                <  1:65866/150‑1 (MQ=255)
                   tCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCg                                                                                                                               <  2:860516/150‑1 (MQ=255)
                     tATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCt                                                                                                                             <  2:1975996/150‑1 (MQ=255)
                          cTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCTGCAACCGCTGAttt                                                                                                                        <  2:447718/150‑1 (MQ=255)
                           tGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTa                                                                                                                       <  1:3713581/150‑1 (MQ=255)
                            gCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCCACCGCTGATTTAc                                                                                                                      >  2:4034563/1‑150 (MQ=255)
                            gCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTAc                                                                                                                      <  2:2602350/150‑1 (MQ=255)
                             cGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACg                                                                                                                     <  1:1647421/150‑1 (MQ=255)
                             cGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACg                                                                                                                     >  2:1791748/1‑150 (MQ=255)
                             cGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACg                                                                                                                     <  2:4138675/150‑1 (MQ=255)
                              gACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACgg                                                                                                                    >  2:314581/1‑150 (MQ=255)
                                cTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGc                                                                                                                  >  1:1757461/1‑150 (MQ=255)
                                cTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGc                                                                                                                  <  1:3675303/150‑1 (MQ=255)
                                       aaaCGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACAt                                                                                                           >  1:3588749/1‑150 (MQ=255)
                                       aaaCGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACAt                                                                                                           >  1:3297631/1‑150 (MQ=255)
                                         aCGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTa                                                                                                         <  1:292117/150‑1 (MQ=255)
                                               gCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACt                                                                                                   <  1:2180530/150‑1 (MQ=255)
                                               gCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACt                                                                                                   <  1:276909/150‑1 (MQ=255)
                                                cTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTg                                                                                                  >  1:4101653/1‑150 (MQ=255)
                                                 tATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTgg                                                                                                 >  1:4522860/1‑150 (MQ=255)
                                                  aTTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGc                                                                                                <  1:2520189/150‑1 (MQ=255)
                                                       aCCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGaa                                                                                           <  1:4266769/150‑1 (MQ=255)
                                                         ctctGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAAtt                                                                                         <  1:948914/150‑1 (MQ=255)
                                                            tGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTggg                                                                                      <  1:2939309/150‑1 (MQ=255)
                                                             gCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGt                                                                                     <  1:573643/150‑1 (MQ=255)
                                                              ccAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTg                                                                                    >  2:3076313/1‑150 (MQ=255)
                                                                aGCGACCTTGAGTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTgcg                                                                                  <  2:4366819/150‑1 (MQ=255)
                                                                  cGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGct                                                                                <  2:2579594/150‑1 (MQ=255)
                                                                  cGAACTAGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTGTATCAGGGCTTTGTTAAAGGGGCACCTCAGCAACCGCTGATTTACGGGCCGACCCTTAAAGACTGGCCGGAATTGGGTGCGct                                                                                <  2:2906533/150‑1 (MQ=255)
                                                                   gAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGctg                                                                               >  1:1040732/1‑150 (MQ=255)
                                                                     aCTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGctgac                                                                             <  2:3907340/150‑1 (MQ=255)
                                                                          aTTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGACTTGGGTGCGCTGACTGACa                                                                        >  1:4149644/1‑150 (MQ=255)
                                                                              cTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAatat                                                                    >  1:3485180/1‑150 (MQ=255)
                                                                              cTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAatat                                                                    >  2:2167342/1‑150 (MQ=255)
                                                                               tGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATc                                                                   >  2:1035197/1‑150 (MQ=255)
                                                                               tGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATc                                                                   <  2:1735256/150‑1 (MQ=255)
                                                                                gggACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCg                                                                  <  1:4226339/150‑1 (MQ=255)
                                                                                     aaCGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTg                                                             >  1:2239648/1‑150 (MQ=255)
                                                                                       cGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGaa                                                           <  1:3887158/150‑1 (MQ=255)
                                                                                       cGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGaa                                                           <  1:2698175/150‑1 (MQ=255)
                                                                                         tGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAg                                                         >  1:1180958/1‑150 (MQ=255)
                                                                                         tGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAg                                                         >  1:1251670/1‑150 (MQ=255)
                                                                                          gCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAgt                                                        <  2:1433749/150‑1 (MQ=255)
                                                                                            cGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAgtgt                                                      <  2:1994958/150‑1 (MQ=255)
                                                                                            cGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAgtgt                                                      >  2:4268893/1‑150 (MQ=255)
                                                                                            cGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAgtgt                                                      >  1:739492/1‑150 (MQ=255)
                                                                                             ggAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGtgtg                                                     <  1:1272587/150‑1 (MQ=255)
                                                                                             ggAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGtgtg                                                     <  1:1288427/150‑1 (MQ=255)
                                                                                              gAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGc                                                    <  2:633836/150‑1 (MQ=255)
                                                                                              gAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGc                                                    <  2:407650/150‑1 (MQ=255)
                                                                                               aGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCt                                                   <  1:2319946/150‑1 (MQ=255)
                                                                                                   cGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGaa                                               <  1:4434775/150‑1 (MQ=255)
                                                                                                    gCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAg                                              <  1:1814959/150‑1 (MQ=255)
                                                                                                      cTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGAt                                            >  2:411418/1‑150 (MQ=255)
                                                                                                        tCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATcc                                          <  1:1691832/150‑1 (MQ=255)
                                                                                                         cGATGTAACTCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCt                                         <  2:2963106/150‑1 (MQ=255)
                                                                                                         cGATGTAACTCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCt                                         <  2:112780/150‑1 (MQ=255)
                                                                                                         cGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCt                                         >  2:1374863/1‑150 (MQ=255)
                                                                                                          gATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTc                                        <  2:4380961/150‑1 (MQ=255)
                                                                                                          gATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTc                                        <  2:3731766/150‑1 (MQ=255)
                                                                                                          gATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTc                                        <  2:4380825/150‑1 (MQ=255)
                                                                                                           aTGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcg                                       <  2:873085/150‑1 (MQ=255)
                                                                                                           aTGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcg                                       <  1:328186/150‑1 (MQ=255)
                                                                                                           aTGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcg                                       <  2:1555874/150‑1 (MQ=255)
                                                                                                            tGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcga                                      >  2:3343424/1‑150 (MQ=255)
                                                                                                            tGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcga                                      >  2:1306832/1‑150 (MQ=255)
                                                                                                             gTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcgac                                     <  1:4380636/150‑1 (MQ=255)
                                                                                                              tAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTcgacg                                    >  1:1450882/1‑150 (MQ=255)
                                                                                                                   ccGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTg                               <  1:3524477/150‑1 (MQ=255)
                                                                                                                   ccGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTg                               <  2:2842675/150‑1 (MQ=255)
                                                                                                                    cGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGa                              >  2:170262/1‑150 (MQ=255)
                                                                                                                       ataAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGacca                           >  1:3220040/1‑150 (MQ=255)
                                                                                                                       ataAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGacca                           <  1:879047/150‑1 (MQ=255)
                                                                                                                          aaaaCCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGaccacca                        <  1:1049735/150‑1 (MQ=255)
                                                                                                                            aaCCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGaccaccacc                      >  1:2790473/1‑150 (MQ=255)
                                                                                                                             aCCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACcg                     <  2:2631010/150‑1 (MQ=255)
                                                                                                                               cGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACcgac                   >  2:1627991/1‑150 (MQ=255)
                                                                                                                               cGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACcgac                   >  2:2556406/1‑150 (MQ=255)
                                                                                                                                gtgtTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACcgacg                  >  1:2712673/1‑150 (MQ=255)
                                                                                                                                gtgtTTATCAGGGCTTTGTAAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGTGTGCGCAGACTGACAAAATCTTCCTGAAAGAGTGCTCTAAGATCCTCGACGAAGTGACCAACACcgacg                  >  2:1853428/1‑150 (MQ=255)
                                                                                                                                  gtTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGaa                >  1:3658292/1‑150 (MQ=255)
                                                                                                                                   tttATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAAc               >  1:3817618/1‑150 (MQ=255)
                                                                                                                                    ttATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACt              >  2:3610734/1‑150 (MQ=255)
                                                                                                                                      aTCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGa            >  2:2056986/1‑150 (MQ=255)
                                                                                                                                      aTCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGa            >  1:1323134/1‑150 (MQ=255)
                                                                                                                                       tCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAt           >  1:1495182/1‑150 (MQ=255)
                                                                                                                                        cAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAtt          >  2:2266063/1‑150 (MQ=255)
                                                                                                                                          gggCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAttcc        >  1:1212957/1‑150 (MQ=255)
                                                                                                                                          gggCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAttcc        >  2:1914736/1‑150 (MQ=255)
                                                                                                                                           ggCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAttcct       >  2:3254492/1‑150 (MQ=255)
                                                                                                                                            gCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAttcctt      <  2:2004420/150‑1 (MQ=255)
                                                                                                                                              tttGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAttccttcc    <  1:2831596/150‑1 (MQ=255)
                                                                                                                                               ttGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCg   >  1:3150440/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCGG  >  CP009273/800341‑800634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCG  >  CP009273/800339‑800633
                                                                                                                                                     |                                                                                                                                                 
GTGGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTT                                                                                                                                                   <  SRR19973080.2751852/150‑1 (MQ=60)
GTGGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTT                                                                                                                                                   <  SRR19973080.2749891/150‑1 (MQ=60)
 TGGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTA                                                                                                                                                  <  SRR19973080.4950/150‑1 (MQ=60)
  GGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAA                                                                                                                                                 <  SRR19973080.3863717/150‑1 (MQ=60)
  GGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAA                                                                                                                                                 <  SRR19973080.3863952/150‑1 (MQ=60)
  GGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAA                                                                                                                                                 <  SRR19973080.4350770/150‑1 (MQ=60)
  GGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAA                                                                                                                                                 >  SRR19973080.4235466/1‑150 (MQ=60)
       TGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGG                                                                                                                                            <  SRR19973080.191294/150‑1 (MQ=60)
         ATGGACGCTCGTTCTATCGCTGCGACAGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCA                                                                                                                                          >  SRR19973080.4385580/1‑150 (MQ=60)
            GACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACT                                                                                                                                       <  SRR19973080.3816646/150‑1 (MQ=60)
            GACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACT                                                                                                                                       <  SRR19973080.1544101/150‑1 (MQ=60)
            GACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACT                                                                                                                                       >  SRR19973080.4212962/1‑150 (MQ=60)
              CGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCA                                                                                                                                     <  SRR19973080.4300297/150‑1 (MQ=60)
              CGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCA                                                                                                                                     <  SRR19973080.4300331/150‑1 (MQ=60)
               GCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACGTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTGTGTTAAAGGGGCAACTCAG                                                                                                                                    >  SRR19973080.3847228/1‑150 (MQ=60)
                CTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGC                                                                                                                                   >  SRR19973080.3588346/1‑150 (MQ=60)
                   GTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAAC                                                                                                                                >  SRR19973080.2454454/1‑150 (MQ=60)
                   GTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAAC                                                                                                                                >  SRR19973080.253322/1‑150 (MQ=60)
                    TTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACC                                                                                                                               <  SRR19973080.2940469/150‑1 (MQ=60)
                    TTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACC                                                                                                                               <  SRR19973080.66627/150‑1 (MQ=60)
                    TTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACC                                                                                                                               <  SRR19973080.66510/150‑1 (MQ=60)
                      CTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGC                                                                                                                             <  SRR19973080.4058650/150‑1 (MQ=60)
                        ATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTG                                                                                                                           >  SRR19973080.2567855/1‑150 (MQ=60)
                           GCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATT                                                                                                                        <  SRR19973080.3538387/150‑1 (MQ=60)
                             TGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTA                                                                                                                      >  SRR19973080.1685355/1‑150 (MQ=60)
                             TGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTA                                                                                                                      <  SRR19973080.3733945/150‑1 (MQ=60)
                               CGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACG                                                                                                                    <  SRR19973080.1657058/150‑1 (MQ=60)
                                 ACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGG                                                                                                                  >  SRR19973080.1821785/1‑150 (MQ=60)
                                  CTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGC                                                                                                                 >  SRR19973080.1767586/1‑150 (MQ=60)
                                  CTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGC                                                                                                                 <  SRR19973080.3695536/150‑1 (MQ=60)
                                  CTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGC                                                                                                                 <  SRR19973080.3695493/150‑1 (MQ=60)
                                       GCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAAC                                                                                                            >  SRR19973080.3456748/1‑150 (MQ=60)
                                         AAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACAT                                                                                                          >  SRR19973080.3608591/1‑150 (MQ=60)
                                         AAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACAT                                                                                                          >  SRR19973080.3608631/1‑150 (MQ=60)
                                         AAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACAT                                                                                                          >  SRR19973080.3315690/1‑150 (MQ=60)
                                           ACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTA                                                                                                        <  SRR19973080.293994/150‑1 (MQ=60)
                                             GGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAA                                                                                                      >  SRR19973080.933563/1‑150 (MQ=60)
                                             GGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTGTGGTAAAGGGGCAACTCAGCAACCGCTGATGTACGGGCCGAACATTAAA                                                                                                      >  SRR19973080.4175665/1‑150 (MQ=60)
                                               TGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGA                                                                                                    <  SRR19973080.1422194/150‑1 (MQ=60)
                                               TGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGA                                                                                                    >  SRR19973080.4392048/1‑150 (MQ=60)
                                                GGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGAC                                                                                                   >  SRR19973080.1256820/1‑150 (MQ=60)
                                                 GCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACT                                                                                                  <  SRR19973080.2192669/150‑1 (MQ=60)
                                                 GCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACT                                                                                                  <  SRR19973080.278732/150‑1 (MQ=60)
                                                  CTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTG                                                                                                 >  SRR19973080.4123949/1‑150 (MQ=60)
                                                   TATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGG                                                                                                >  SRR19973080.4546176/1‑150 (MQ=60)
                                                   TATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGG                                                                                                >  SRR19973080.4547233/1‑150 (MQ=60)
                                                   TATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGG                                                                                                <  SRR19973080.55195/150‑1 (MQ=60)
                                                    ATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGC                                                                                               <  SRR19973080.2534059/150‑1 (MQ=60)
                                                    ATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGC                                                                                               <  SRR19973080.2535827/150‑1 (MQ=60)
                                                         ACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAA                                                                                          <  SRR19973080.4289923/150‑1 (MQ=60)
                                                         ACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAA                                                                                          <  SRR19973080.4290060/150‑1 (MQ=60)
                                                          CCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAAT                                                                                         <  SRR19973080.2833657/150‑1 (MQ=60)
                                                           CTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATT                                                                                        <  SRR19973080.954688/150‑1 (MQ=60)
                                                              TGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGG                                                                                     <  SRR19973080.2955283/150‑1 (MQ=60)
                                                               GCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGT                                                                                    <  SRR19973080.577231/150‑1 (MQ=60)
                                                                 CAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGC                                                                                  <  SRR19973080.3696534/150‑1 (MQ=60)
                                                                  AGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCG                                                                                 <  SRR19973080.1292311/150‑1 (MQ=60)
                                                                  AGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCG                                                                                 >  SRR19973080.1442595/1‑150 (MQ=60)
                                                                    CGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCT                                                                               <  SRR19973080.3967773/150‑1 (MQ=60)
                                                                     GAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTG                                                                              >  SRR19973080.1046897/1‑150 (MQ=60)
                                                                     GAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTG                                                                              >  SRR19973080.1046879/1‑150 (MQ=60)
                                                                        CTTGATTGCTGTGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACT                                                                           <  SRR19973080.2036896/150‑1 (MQ=60)
                                                                          TGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGA                                                                         >  SRR19973080.75363/1‑150 (MQ=60)
                                                                            ATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGACTTGGGTGCGCTGACTGACA                                                                       >  SRR19973080.4172062/1‑150 (MQ=60)
                                                                            ATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACA                                                                       >  SRR19973080.1795071/1‑150 (MQ=60)
                                                                            ATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACA                                                                       >  SRR19973080.4172093/1‑150 (MQ=60)
                                                                             TTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAA                                                                      >  SRR19973080.954177/1‑150 (MQ=60)
                                                                               GCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATA                                                                    <  SRR19973080.2862234/150‑1 (MQ=60)
                                                                                CTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATAT                                                                   >  SRR19973080.2315691/1‑150 (MQ=60)
                                                                                CTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATAT                                                                   >  SRR19973080.3504380/1‑150 (MQ=60)
                                                                                  GGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCG                                                                 >  SRR19973080.2789859/1‑150 (MQ=60)
                                                                                  GGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCG                                                                 <  SRR19973080.4249343/150‑1 (MQ=60)
                                                                                     ACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCC                                                              <  SRR19973080.2982149/150‑1 (MQ=60)
                                                                                       AACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTG                                                            >  SRR19973080.2251943/1‑150 (MQ=60)
                                                                                       AACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTG                                                            <  SRR19973080.4478300/150‑1 (MQ=60)
                                                                                        ACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGA                                                           <  SRR19973080.435169/150‑1 (MQ=60)
                                                                                         CGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAA                                                          <  SRR19973080.3908586/150‑1 (MQ=60)
                                                                                         CGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAA                                                          <  SRR19973080.2713588/150‑1 (MQ=60)
                                                                                         CGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAA                                                          <  SRR19973080.2712939/150‑1 (MQ=60)
                                                                                           TGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAG                                                        >  SRR19973080.1259414/1‑150 (MQ=60)
                                                                                           TGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAG                                                        >  SRR19973080.1259246/1‑150 (MQ=60)
                                                                                           TGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAG                                                        >  SRR19973080.1188106/1‑150 (MQ=60)
                                                                                           TGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAG                                                        >  SRR19973080.1188020/1‑150 (MQ=60)
                                                                                              CGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGT                                                     >  SRR19973080.743706/1‑150 (MQ=60)
                                                                                               GGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTG                                                    <  SRR19973080.1296219/150‑1 (MQ=60)
                                                                                               GGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTG                                                    <  SRR19973080.4159261/150‑1 (MQ=60)
                                                                                               GGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTG                                                    <  SRR19973080.1280292/150‑1 (MQ=60)
                                                                                                 agtacgccttcgatgttttttttTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCT                                                  <  SRR19973080.3301633/127‑1 (MQ=60)
                                                                                                 AGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCT                                                  <  SRR19973080.2332819/150‑1 (MQ=60)
                                                                                                 AGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCT                                                  <  SRR19973080.2334435/150‑1 (MQ=60)
                                                                                                     CGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAA                                              <  SRR19973080.4458581/150‑1 (MQ=60)
                                                                                                     CGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAA                                              <  SRR19973080.4458858/150‑1 (MQ=60)
                                                                                                      GCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAG                                             <  SRR19973080.1825241/150‑1 (MQ=60)
                                                                                                      GCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAG                                             <  SRR19973080.1825246/150‑1 (MQ=60)
                                                                                                          TCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCC                                         <  SRR19973080.1701687/150‑1 (MQ=60)
                                                                                                            GATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTC                                       <  SRR19973080.1119624/150‑1 (MQ=60)
                                                                                                             ATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCG                                      <  SRR19973080.330219/150‑1 (MQ=60)
                                                                                                             ATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCG                                      <  SRR19973080.330187/150‑1 (MQ=60)
                                                                                                             ATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCG                                      <  SRR19973080.583244/150‑1 (MQ=60)
                                                                                                               GTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGAC                                    <  SRR19973080.4404510/150‑1 (MQ=60)
                                                                                                                TAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACG                                   >  SRR19973080.1459303/1‑150 (MQ=60)
                                                                                                                     CCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTG                              <  SRR19973080.3543962/150‑1 (MQ=60)
                                                                                                                         ATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCA                          <  SRR19973080.2773961/150‑1 (MQ=60)
                                                                                                                         ATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCA                          <  SRR19973080.884426/150‑1 (MQ=60)
                                                                                                                         ATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCA                          >  SRR19973080.3238966/1‑150 (MQ=60)
                                                                                                                         ATAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCA                          >  SRR19973080.3237803/1‑150 (MQ=60)
                                                                                                                          TAAAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCAC                         >  SRR19973080.1207152/1‑150 (MQ=60)
                                                                                                                          TAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCAC                         >  SRR19973080.2228619/1‑150 (MQ=60)
                                                                                                                           AAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACC                        >  SRR19973080.1490358/1‑150 (MQ=60)
                                                                                                                            AAAACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCA                       <  SRR19973080.1055906/150‑1 (MQ=60)
                                                                                                                              AACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACC                     >  SRR19973080.2805866/1‑150 (MQ=60)
                                                                                                                              AACCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACC                     >  SRR19973080.1748445/1‑150 (MQ=60)
                                                                                                                                CCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGA                   >  SRR19973080.4277021/1‑150 (MQ=60)
                                                                                                                                CCGTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGA                   >  SRR19973080.1913066/1‑150 (MQ=60)
                                                                                                                                  GTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACG                 >  SRR19973080.2727546/1‑150 (MQ=60)
                                                                                                                                  GTGTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACG                 >  SRR19973080.2727556/1‑150 (MQ=60)
                                                                                                                                    GTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAA               >  SRR19973080.3679068/1‑150 (MQ=60)
                                                                                                                                    GTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAA               >  SRR19973080.3678470/1‑150 (MQ=60)
                                                                                                                                    GTTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAA               <  SRR19973080.351912/150‑1 (MQ=60)
                                                                                                                                     TTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAAC              <  SRR19973080.1521737/150‑1 (MQ=60)
                                                                                                                                     TTTATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAAC              >  SRR19973080.3838516/1‑150 (MQ=60)
                                                                                                                                       TATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTG            >  SRR19973080.676228/1‑150 (MQ=60)
                                                                                                                                        ATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGA           >  SRR19973080.1265683/1‑150 (MQ=60)
                                                                                                                                        ATCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGA           >  SRR19973080.3748814/1‑150 (MQ=60)
                                                                                                                                        ATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGA           >  SRR19973080.1330974/1‑150 (MQ=60)
                                                                                                                                        ATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGA           >  SRR19973080.1331125/1‑150 (MQ=60)
                                                                                                                                        ATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGA           >  SRR19973080.2372744/1‑150 (MQ=60)
                                                                                                                                         TCAGGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAT          >  SRR19973080.1503696/1‑150 (MQ=60)
                                                                                                                                            GGGCTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCC       >  SRR19973080.1220292/1‑150 (MQ=60)
                                                                                                                                               CTTTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTC    >  SRR19973080.3080525/1‑150 (MQ=60)
                                                                                                                                                TTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCC   <  SRR19973080.2847796/150‑1 (MQ=60)
                                                                                                                                                TTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCC   <  SRR19973080.2847161/150‑1 (MQ=60)
                                                                                                                                                 TTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCG  >  SRR19973080.3167828/1‑150 (MQ=60)
                                                                                                                                                 TTGTTAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCG  >  SRR19973080.3167794/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
GTGGCGTTGATGGACGCTCGTTCTATCGCTGCGACTGCGGCAAACGGTGGCTATTTAACCTCTGCCAGCGAACTTGATTGCTGGGACAACGTGCCGGAGTACGCCTTCGATGTAACGCCGTATAAAAACCGTGTTTATCAGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAACATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCG  >  CP009273/800339‑800633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑