Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 997,965 G→T 10.0% D235Y (GAT→TAT)  elfG → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273997,9650GT10.0% 1104.4 / 67.6 306D235Y (GAT→TAT) elfGputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (143/132);  new base T (14/17);  total (157/149)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTAT  >  CP009273/997816‑998114
                                                                                                                                                     |                                                                                                                                                     
cgcgATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTg                                                                                                                                                       <  1:2855592/150‑1 (MQ=255)
cgcgATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTg                                                                                                                                                       >  2:1015672/1‑150 (MQ=255)
   gATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATg                                                                                                                                                    <  1:3395219/150‑1 (MQ=255)
   gATTATATGAATGCTACAACATACCCGCAGGTGAGTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATg                                                                                                                                                    <  1:1581210/150‑1 (MQ=255)
       atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                                >  1:1440512/1‑150 (MQ=255)
       atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                                >  1:3724491/1‑150 (MQ=255)
        tatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTc                                                                                                                                               <  2:269211/150‑1 (MQ=255)
         atGAATGCTACAACATACCCGCCGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCg                                                                                                                                              <  2:3258391/150‑1 (MQ=255)
         atGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCg                                                                                                                                              <  2:638268/150‑1 (MQ=255)
           gAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCt                                                                                                                                            <  2:4334866/150‑1 (MQ=255)
            aaTGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTg                                                                                                                                           >  1:4098319/1‑150 (MQ=255)
                 tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAAt                                                                                                                                      >  2:1093019/1‑150 (MQ=255)
                 tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAAt                                                                                                                                      <  1:3759680/150‑1 (MQ=255)
                 tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAAt                                                                                                                                      >  1:3333546/1‑150 (MQ=255)
                  acaacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAAtt                                                                                                                                     <  1:2720319/150‑1 (MQ=255)
                      caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCg                                                                                                                                 >  2:3950176/1‑150 (MQ=255)
                      caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCg                                                                                                                                 >  1:1960357/1‑150 (MQ=255)
                       aTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCgt                                                                                                                                >  2:2587261/1‑150 (MQ=255)
                        tACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCgtg                                                                                                                               >  2:1559028/1‑150 (MQ=255)
                          cccGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgtt                                                                                                                             >  2:251417/1‑150 (MQ=255)
                           ccGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttg                                                                                                                            >  2:3311318/1‑150 (MQ=255)
                           ccGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttg                                                                                                                            <  2:2233391/150‑1 (MQ=255)
                            cGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttgt                                                                                                                           <  2:278538/150‑1 (MQ=255)
                             gCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGGATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTa                                                                                                                          <  2:3038937/150‑1 (MQ=255)
                                ggTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAggg                                                                                                                       <  2:2341787/150‑1 (MQ=255)
                                  tGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCa                                                                                                                     <  1:3267032/150‑1 (MQ=255)
                                   gATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCAt                                                                                                                    <  1:3871788/150‑1 (MQ=255)
                                       ccTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAc                                                                                                                <  1:707626/150‑1 (MQ=255)
                                          gCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCCATTTCCGTGTTGTAGGGCATAAACctc                                                                                                             >  2:3172196/1‑150 (MQ=255)
                                             cGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTg                                                                                                          <  1:2353203/150‑1 (MQ=255)
                                                   cAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCa                                                                                                    <  1:3591225/150‑1 (MQ=255)
                                                    aGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAg                                                                                                   <  2:373965/150‑1 (MQ=255)
                                                      gTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGTCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGaa                                                                                                 <  2:2041819/150‑1 (MQ=255)
                                                       tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                                <  1:2767194/150‑1 (MQ=255)
                                                       tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                                <  1:4124031/150‑1 (MQ=255)
                                                       tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                                <  2:4269500/150‑1 (MQ=255)
                                                        acacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACg                                                                                               >  2:2121384/1‑150 (MQ=255)
                                                        acacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACg                                                                                               <  2:1537374/150‑1 (MQ=255)
                                                         cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                              >  1:1377766/1‑150 (MQ=255)
                                                          acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                             >  1:1059568/1‑150 (MQ=255)
                                                          acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                             >  1:3495960/1‑150 (MQ=255)
                                                            cGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAg                                                                                           <  1:2501219/150‑1 (MQ=255)
                                                              gTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGaa                                                                                         >  1:1815160/1‑150 (MQ=255)
                                                              gTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGaa                                                                                         >  2:2999347/1‑150 (MQ=255)
                                                               ttttAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAAc                                                                                        <  1:2587064/150‑1 (MQ=255)
                                                                tttAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACt                                                                                       >  2:1987011/1‑150 (MQ=255)
                                                                tttAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACt                                                                                       >  2:1292440/1‑150 (MQ=255)
                                                                 ttAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACtt                                                                                      >  1:1316428/1‑150 (MQ=255)
                                                                         ttACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc                                                                              >  2:3558211/1‑150 (MQ=255)
                                                                         ttACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc                                                                              >  2:1273875/1‑150 (MQ=255)
                                                                          tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCa                                                                             <  2:568904/150‑1 (MQ=255)
                                                                           aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAg                                                                            >  1:254653/1‑150 (MQ=255)
                                                                             cTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc                                                                          >  2:3672843/1‑150 (MQ=255)
                                                                               gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAg                                                                        <  1:2817904/150‑1 (MQ=255)
                                                                               gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAg                                                                        <  2:76182/150‑1 (MQ=255)
                                                                                  tGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtg                                                                     >  1:3095889/1‑150 (MQ=255)
                                                                                   ggTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtgt                                                                    >  1:3321900/1‑150 (MQ=255)
                                                                                    gTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTa                                                                   >  1:968500/1‑150 (MQ=255)
                                                                                     tACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTaa                                                                  >  2:1573723/1‑150 (MQ=255)
                                                                                     tACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTaa                                                                  <  1:834940/150‑1 (MQ=255)
                                                                                           cAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACggg                                                            >  2:4432270/1‑150 (MQ=255)
                                                                                             aTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaa                                                          >  1:2641311/1‑150 (MQ=255)
                                                                                               tCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAc                                                        <  2:4142147/150‑1 (MQ=255)
                                                                                                cACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcg                                                       >  1:3796988/1‑150 (MQ=255)
                                                                                                 aCTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgc                                                      >  1:3602124/1‑150 (MQ=255)
                                                                                                 aCTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgc                                                      >  1:4075413/1‑150 (MQ=255)
                                                                                                      gCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAGCACGGGAAACGCGGGAt                                                 <  1:4459427/150‑1 (MQ=255)
                                                                                                      gCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAt                                                 <  1:671460/150‑1 (MQ=255)
                                                                                                       cTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAtt                                                >  1:2143992/1‑150 (MQ=255)
                                                                                                         cATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAg                                              <  2:884675/150‑1 (MQ=255)
                                                                                                           tGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGtt                                            <  1:1919690/150‑1 (MQ=255)
                                                                                                           tGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGtt                                            <  2:1529054/150‑1 (MQ=255)
                                                                                                                cGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGt                                       <  2:791000/150‑1 (MQ=255)
                                                                                                                cGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGt                                       <  2:4367802/150‑1 (MQ=255)
                                                                                                                  tCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCa                                     <  2:1960357/150‑1 (MQ=255)
                                                                                                                    aaTGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAc                                   <  2:4193226/150‑1 (MQ=255)
                                                                                                                    aaTGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAc                                   >  1:684329/1‑150 (MQ=255)
                                                                                                                    aaTGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAc                                   >  1:1677559/1‑150 (MQ=255)
                                                                                                                     aTGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAcc                                  <  1:2948911/150‑1 (MQ=255)
                                                                                                                     aTGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAcc                                  <  1:2445365/150‑1 (MQ=255)
                                                                                                                       gCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTg                                <  1:3664782/150‑1 (MQ=255)
                                                                                                                          ggAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGt                             <  2:1215725/150‑1 (MQ=255)
                                                                                                                          ggAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGt                             <  2:1353078/150‑1 (MQ=255)
                                                                                                                            agagaCAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCt                           <  1:127060/150‑1 (MQ=255)
                                                                                                                             gagaCAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTg                          <  2:3495960/150‑1 (MQ=255)
                                                                                                                                aCAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACg                       >  1:3848235/1‑150 (MQ=255)
                                                                                                                                 cAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg                      <  2:682892/150‑1 (MQ=255)
                                                                                                                                 cAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg                      <  2:2943861/150‑1 (MQ=255)
                                                                                                                                   aTTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCa                    >  1:587946/1‑150 (MQ=255)
                                                                                                                                     tGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAc                  >  1:1860636/1‑150 (MQ=255)
                                                                                                                                       aaGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACca                <  2:1593762/150‑1 (MQ=255)
                                                                                                                                         gTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcaca              <  1:2029661/150‑1 (MQ=255)
                                                                                                                                               ttAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc        <  2:3788876/150‑1 (MQ=255)
                                                                                                                                               ttAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc        <  1:165521/150‑1 (MQ=255)
                                                                                                                                               ttAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc        >  2:2078688/1‑150 (MQ=255)
                                                                                                                                                tAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa       <  1:1293092/150‑1 (MQ=255)
                                                                                                                                                tAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa       >  2:1065090/1‑150 (MQ=255)
                                                                                                                                                  ggTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGc     >  2:2536817/1‑150 (MQ=255)
                                                                                                                                                  ggTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGc     <  1:1559028/150‑1 (MQ=255)
                                                                                                                                                     gATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTAt  <  1:10275/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTAT  >  CP009273/997816‑998114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A