Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F368 I0 R1	1472	214.4	7116750	95.9%	6824963	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	997,965	G→T	100%	D235Y (GAT→TAT)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	997,965	0	G	T	96.6%	638.5 / 11.9	209	D235Y (GAT→TAT)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand): ref base G (2/5); new base T (107/95); total (109/100)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 209 total aligned reads displayed.

GATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCA  >  CP009273/997819‑998109

gATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGa                                                                                                                                                 <  2:2002313/148‑1 (MQ=255)

gATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGa                                                                                                                                                 <  2:176837/148‑1 (MQ=255)

  ttATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATg                                                                                                                                               <  1:2358047/148‑1 (MQ=255)

   tataTGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGt                                                                                                                                              <  2:3172134/148‑1 (MQ=255)

    atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTc                                                                                                                                             <  1:1031566/148‑1 (MQ=255)

    atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTc                                                                                                                                             >  1:135327/1‑148 (MQ=255)

     tatgtatgCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGCTACACCGGTTTTAGTGTATTACCTGTCTGTTACGATCAATTCACTTGGCTCCTGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCt                                                                                                                                            <  2:3263639/148‑1 (MQ=255)

     tatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCt                                                                                                                                            <  1:2635884/148‑1 (MQ=255)

      atGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                           >  1:2602317/1‑148 (MQ=255)

      atGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                           >  2:773237/1‑148 (MQ=255)

        gAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCg                                                                                                                                         <  2:1892278/148‑1 (MQ=255)

           tGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTg                                                                                                                                      <  2:157534/148‑1 (MQ=255)

           tGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTg                                                                                                                                      <  1:1922400/148‑1 (MQ=255)

            gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGc                                                                                                                                     <  2:1347859/148‑1 (MQ=255)

             cTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGcc                                                                                                                                    >  2:652618/1‑148 (MQ=255)

                caacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAt                                                                                                                                 <  2:373774/148‑1 (MQ=255)

                caacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAt                                                                                                                                 <  1:2206661/148‑1 (MQ=255)

                  acaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAttt                                                                                                                               >  1:193691/1‑148 (MQ=255)

                   caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTc                                                                                                                              >  2:1679807/1‑148 (MQ=255)

                   caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTc                                                                                                                              <  1:909401/148‑1 (MQ=255)

                       cccGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCgtg                                                                                                                          <  2:1091910/148‑1 (MQ=255)

                         cGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGtgtt                                                                                                                        >  1:2452080/1‑148 (MQ=255)

                         cGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGtgtt                                                                                                                        <  1:660396/148‑1 (MQ=255)

                          gCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCACTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGtgttg                                                                                                                       <  2:1875911/148‑1 (MQ=255)

                           cAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGtgttgt                                                                                                                      <  1:1075735/148‑1 (MQ=255)

                           cAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttgt                                                                                                                      >  1:1651818/1‑148 (MQ=255)

                              gTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAgg                                                                                                                   >  2:266854/1‑148 (MQ=255)

                              gTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAgg                                                                                                                   >  2:2786401/1‑148 (MQ=255)

                               tGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAggg                                                                                                                  >  1:3552078/1‑148 (MQ=255)

                                 aTTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCa                                                                                                                >  1:853924/1‑148 (MQ=255)

                                   tCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATa                                                                                                              <  1:3184230/148‑1 (MQ=255)

                                    ccTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                                                                                                             <  1:50986/148‑1 (MQ=255)

                                        cACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACct                                                                                                         <  2:2464903/148‑1 (MQ=255)

                                              tACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTggg                                                                                                   >  2:3518775/1‑148 (MQ=255)

                                                cAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGc                                                                                                 <  1:1784579/148‑1 (MQ=255)

                                                 aGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGcc                                                                                                <  1:2619829/148‑1 (MQ=255)

                                                    tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGa                                                                                             <  1:2803364/148‑1 (MQ=255)

                                                       acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACg                                                                                          <  1:3416451/148‑1 (MQ=255)

                                                        ccGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                         >  1:3325483/1‑148 (MQ=255)

                                                        ccGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                         >  1:1153192/1‑148 (MQ=255)

                                                         cGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                        >  2:569594/1‑148 (MQ=255)

                                                                  tgtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc                                                                               >  2:905558/1‑148 (MQ=255)

                                                                  tgtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc                                                                               <  2:1535387/148‑1 (MQ=255)

                                                                  tgtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc                                                                               >  1:1963217/1‑148 (MQ=255)

                                                                     aTTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt                                                                            <  1:980542/148‑1 (MQ=255)

                                                                     aTTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt                                                                            <  1:2756348/148‑1 (MQ=255)

                                                                      ttACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAtt                                                                           >  2:2042221/1‑148 (MQ=255)

                                                                      ttACCTGTCTGGTACGATCAAGTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAtt                                                                           <  1:3124433/148‑1 (MQ=255)

                                                                       tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTc                                                                          >  1:1730799/1‑148 (MQ=255)

                                                                       tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTc                                                                          <  2:3439631/148‑1 (MQ=255)

                                                                       tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTc                                                                          >  2:2330265/1‑148 (MQ=255)

                                                                         ccTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCa                                                                        >  1:162627/1‑148 (MQ=255)

                                                                          cTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAg                                                                       <  1:2276702/148‑1 (MQ=255)

                                                                           tGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt                                                                      >  1:3208769/1‑148 (MQ=255)

                                                                           tGTCTGGTACGATCAATTCACTTAGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt                                                                      <  1:2158717/148‑1 (MQ=255)

                                                                            ttCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATAGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc                                                                     >  2:2218391/2‑148 (MQ=255)

                                                                              cTGGTACGATCAATTCACTTGGCGCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAg                                                                   >  1:2393730/1‑148 (MQ=255)

                                                                                ggTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgt                                                                 <  1:2042221/148‑1 (MQ=255)

                                                                                   aCGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTa                                                              >  2:1232062/1‑148 (MQ=255)

                                                                                   aCGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTa                                                              >  2:2489445/1‑148 (MQ=255)

                                                                                    cGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTaa                                                             >  1:569337/1‑148 (MQ=255)

                                                                                     gATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAac                                                            >  1:2532658/1‑148 (MQ=255)

                                                                                      aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAaca                                                           >  2:525282/1‑148 (MQ=255)

                                                                                      aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAaca                                                           >  2:3502862/1‑148 (MQ=255)

                                                                                        cAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACg                                                         <  1:533841/148‑1 (MQ=255)

                                                                                           ttCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGa                                                      >  2:2479699/1‑148 (MQ=255)

                                                                                            tCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaa                                                     <  1:2355428/148‑1 (MQ=255)

                                                                                              aCTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTATTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTGGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAc                                                   >  2:829303/1‑148 (MQ=255)

                                                                                                 tGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgcg                                                >  1:771276/1‑148 (MQ=255)

                                                                                                 tGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgcg                                                >  1:844623/1‑148 (MQ=255)

                                                                                                  ggCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCgg                                               <  1:2575377/148‑1 (MQ=255)

                                                                                                      cATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAtt                                           >  1:233425/1‑148 (MQ=255)

                                                                                                       aTGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTa                                          >  1:371851/1‑148 (MQ=255)

                                                                                                        tGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAg                                         >  1:1597160/1‑148 (MQ=255)

                                                                                                         gTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGt                                        >  2:1932873/1‑148 (MQ=255)

                                                                                                         gTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGt                                        >  1:3120142/1‑148 (MQ=255)

                                                                                                            ccGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTaa                                     <  1:780641/148‑1 (MQ=255)

                                                                                                            ccGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTaa                                     <  1:3114149/148‑1 (MQ=255)

                                                                                                            ccGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATACCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTaa                                     <  1:1221725/148‑1 (MQ=255)

                                                                                                               tCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGACACGCGGGATTAGTTAATGt                                  >  2:1816372/1‑148 (MQ=255)

                                                                                                               tCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGt                                  <  1:3238702/148‑1 (MQ=255)

                                                                                                                  aTGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAACTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCaa                               <  1:1630662/148‑1 (MQ=255)

                                                                                                                   tGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAc                              >  2:1489639/1‑148 (MQ=255)

                                                                                                                       ggAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGa                          >  1:642115/1‑148 (MQ=255)

                                                                                                                        gagagaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAg                         <  2:2107838/148‑1 (MQ=255)

                                                                                                                         agagaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGt                        >  2:1254893/1‑148 (MQ=255)

                                                                                                                         agagaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGt                        <  2:3195394/148‑1 (MQ=255)

                                                                                                                           agaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGAGTTGTATGGCATAAACCTCTTGGGGCCAGAACGGCAGAACATGCAATTCCAGTCAGGTGTAACACCGGAAACGCGGGATTAGTTAAAGTCAACCTTAGTCt                      >  1:817758/1‑148 (MQ=255)

                                                                                                                             aCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGa                    <  2:431810/148‑1 (MQ=255)

                                                                                                                              cAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAc                   >  1:2807962/1‑148 (MQ=255)

                                                                                                                               aaTTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACg                  >  1:2936002/1‑148 (MQ=255)

                                                                                                                                aTTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg                 >  2:2236856/1‑148 (MQ=255)

                                                                                                                                 ttGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGc                >  1:328040/1‑148 (MQ=255)

                                                                                                                                  tGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCa               >  1:2667679/1‑148 (MQ=255)

                                                                                                                                    aaGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAc             >  1:139798/1‑148 (MQ=255)

                                                                                                                                     aGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAcc            >  1:799047/1‑148 (MQ=255)

                                                                                                                                          aTTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGa       >  1:3526640/1‑148 (MQ=255)

                                                                                                                                           tttAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAc      >  1:2063881/1‑148 (MQ=255)

                                                                                                                                            ttAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcc     <  2:2844583/148‑1 (MQ=255)

                                                                                                                                               ggTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa  >  2:2920610/1‑148 (MQ=255)

GATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCA  >  CP009273/997819‑998109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTA  >  CP009273/997817‑998113

GCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTA                                                                                                                                                     <  SRR19973067.2261467/150‑1 (MQ=60)

GCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTA                                                                                                                                                     <  SRR19973067.2261461/150‑1 (MQ=60)

    TTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTC                                                                                                                                                 <  SRR19973067.905885/150‑1 (MQ=60)

    TTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTC                                                                                                                                                 >  SRR19973067.136534/1‑150 (MQ=60)

    TTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTC                                                                                                                                                 <  SRR19973067.2373424/150‑1 (MQ=60)

      ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                               <  SRR19973067.732010/150‑1 (MQ=60)

      ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                               >  SRR19973067.1677488/1‑150 (MQ=60)

      ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                               <  SRR19973067.1038663/150‑1 (MQ=60)

      ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                               >  SRR19973067.2619240/1‑150 (MQ=60)

      ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                               >  SRR19973067.2619223/1‑150 (MQ=60)

       TATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTC                                                                                                                                              <  SRR19973067.2652937/150‑1 (MQ=60)

       TATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTC                                                                                                                                              <  SRR19973067.1947198/150‑1 (MQ=60)

       TATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTC                                                                                                                                              <  SRR19973067.810633/150‑1 (MQ=60)

        ATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCG                                                                                                                                             >  SRR19973067.20804/1‑150 (MQ=60)

             TGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCC                                                                                                                                        <  SRR19973067.1935516/150‑1 (MQ=60)

             TGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCC                                                                                                                                        <  SRR19973067.960438/150‑1 (MQ=60)

                  CAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTT                                                                                                                                   <  SRR19973067.2219028/150‑1 (MQ=60)

                  CAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTT                                                                                                                                   <  SRR19973067.2221376/150‑1 (MQ=60)

                  CAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTT                                                                                                                                   <  SRR19973067.3242134/150‑1 (MQ=60)

                  CAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTT                                                                                                                                   >  SRR19973067.195187/1‑150 (MQ=60)

                   AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                                  >  SRR19973067.2377551/1‑150 (MQ=60)

                   AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                                  <  SRR19973067.3572092/150‑1 (MQ=60)

                   AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                                  >  SRR19973067.3280026/1‑150 (MQ=60)

                     CATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCG                                                                                                                                <  SRR19973067.3064486/150‑1 (MQ=60)

                     CATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCG                                                                                                                                <  SRR19973067.3216927/150‑1 (MQ=60)

                     CATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCG                                                                                                                                <  SRR19973067.915434/150‑1 (MQ=60)

                       TACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTG                                                                                                                              <  SRR19973067.2866459/150‑1 (MQ=60)

                         CCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTT                                                                                                                            >  SRR19973067.2468198/1‑150 (MQ=60)

                          CCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTG                                                                                                                           <  SRR19973067.824401/150‑1 (MQ=60)

                           CGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGT                                                                                                                          <  SRR19973067.664891/150‑1 (MQ=60)

                           CGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGT                                                                                                                          <  SRR19973067.1647837/150‑1 (MQ=60)

                           CGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGT                                                                                                                          >  SRR19973067.1663195/1‑150 (MQ=60)

                             CAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAG                                                                                                                        >  SRR19973067.2521085/1‑150 (MQ=60)

                             CAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAG                                                                                                                        <  SRR19973067.1083111/150‑1 (MQ=60)

                               GGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGG                                                                                                                      >  SRR19973067.2862842/1‑150 (MQ=60)

                               GGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGG                                                                                                                      >  SRR19973067.3574478/1‑150 (MQ=60)

                                 TGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCA                                                                                                                    >  SRR19973067.859685/1‑150 (MQ=60)

                                     TCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAA                                                                                                                <  SRR19973067.3204369/150‑1 (MQ=60)

                                      CCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAC                                                                                                               <  SRR19973067.51647/150‑1 (MQ=60)

                                          CACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCT                                                                                                           >  SRR19973067.2623716/1‑150 (MQ=60)

                                          CACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCT                                                                                                           <  SRR19973067.1821606/150‑1 (MQ=60)

                                           ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTT                                                                                                          <  SRR19973067.2081102/150‑1 (MQ=60)

                                           ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTT                                                                                                          <  SRR19973067.637179/150‑1 (MQ=60)

                                            CGACGACAGGTACTCCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTG                                                                                                         <  SRR19973067.3314907/150‑1 (MQ=60)

                                             GACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGG                                                                                                        >  SRR19973067.231804/1‑150 (MQ=60)

                                                  CAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCA                                                                                                   <  SRR19973067.1796631/150‑1 (MQ=60)

                                                  CAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCA                                                                                                   <  SRR19973067.538261/150‑1 (MQ=60)

                                                   AGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAG                                                                                                  <  SRR19973067.2636825/150‑1 (MQ=60)

                                                      TACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAC                                                                                               <  SRR19973067.2821440/150‑1 (MQ=60)

                                                      TACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAC                                                                                               <  SRR19973067.2821426/150‑1 (MQ=60)

                                                      TACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAC                                                                                               >  SRR19973067.1213423/1‑150 (MQ=60)

                                                      TACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAC                                                                                               <  SRR19973067.2423918/150‑1 (MQ=60)

                                                      TACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAC                                                                                               <  SRR19973067.2371033/150‑1 (MQ=60)

                                                       ACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACG                                                                                              <  SRR19973067.1809488/150‑1 (MQ=60)

                                                       ACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGAGTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCGAGAACG                                                                                              >  SRR19973067.792775/1‑150 (MQ=60)

                                                        CACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGG                                                                                             >  SRR19973067.1160900/1‑150 (MQ=60)

                                                        CACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGG                                                                                             >  SRR19973067.3346518/1‑150 (MQ=60)

                                                         ACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGC                                                                                            <  SRR19973067.3438196/150‑1 (MQ=60)

                                                           CGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAG                                                                                          <  SRR19973067.2047966/150‑1 (MQ=60)

                                                               TTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTATTGGGGCCAGAACGGCAGAACT                                                                                      >  SRR19973067.532280/1‑150 (MQ=60)

                                                                TTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTT                                                                                     <  SRR19973067.3357525/150‑1 (MQ=60)

                                                                  AGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGC                                                                                   >  SRR19973067.1976554/1‑150 (MQ=60)

                                                                       ATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTC                                                                              >  SRR19973067.1742653/1‑150 (MQ=60)

                                                                       ATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTC                                                                              <  SRR19973067.2774158/150‑1 (MQ=60)

                                                                       ATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTC                                                                              <  SRR19973067.987275/150‑1 (MQ=60)

                                                                        TTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCC                                                                             <  SRR19973067.3144239/150‑1 (MQ=60)

                                                                        TTACCTGTCTGGTACGATCAAGTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCC                                                                             <  SRR19973067.3144293/150‑1 (MQ=60)

                                                                         TACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCA                                                                            >  SRR19973067.163966/1‑150 (MQ=60)

                                                                         TACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCA                                                                            >  SRR19973067.265606/1‑150 (MQ=60)

                                                                         TACCTGTCTGGTACGATCAATTCACTAGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCA                                                                            >  SRR19973067.1261824/1‑150 (MQ=60)

                                                                           CCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGT                                                                          >  SRR19973067.3228991/1‑150 (MQ=60)

                                                                            CTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTC                                                                         <  SRR19973067.2291758/150‑1 (MQ=60)

                                                                            CTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTC                                                                         <  SRR19973067.2291765/150‑1 (MQ=60)

                                                                             TGTCTGGTACGATCAATTCACTTAGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCA                                                                        <  SRR19973067.2173151/150‑1 (MQ=60)

                                                                              GTCTGGTACGATCAATTCACTTGGCGCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAG                                                                       >  SRR19973067.2409310/1‑150 (MQ=60)

                                                                                 TGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTG                                                                    >  SRR19973067.46566/1‑150 (MQ=60)

                                                                                  GGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGT                                                                   <  SRR19973067.2055881/150‑1 (MQ=60)

                                                                                    TACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAA                                                                 >  SRR19973067.573032/1‑150 (MQ=60)

                                                                                     ACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAAC                                                                >  SRR19973067.2549509/1‑150 (MQ=60)

                                                                                     ACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAAC                                                                >  SRR19973067.2549249/1‑150 (MQ=60)

                                                                                          CAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGG                                                           <  SRR19973067.537417/150‑1 (MQ=60)

                                                                                            ATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAA                                                         >  SRR19973067.3182275/1‑150 (MQ=60)

                                                                                              TCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAC                                                       <  SRR19973067.2370035/150‑1 (MQ=60)

                                                                                              TCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAC                                                       <  SRR19973067.2370792/150‑1 (MQ=60)

                                                                                              TCACTTGGCTCATGGTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAC                                                       <  SRR19973067.1531490/150‑1 (MQ=60)

                                                                                                 CTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCG                                                    >  SRR19973067.776621/1‑150 (MQ=60)

                                                                                                 CTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCG                                                    >  SRR19973067.850247/1‑150 (MQ=60)

                                                                                                 CTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCG                                                    >  SRR19973067.850344/1‑150 (MQ=60)

                                                                                                 CTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCG                                                    >  SRR19973067.2641223/1‑150 (MQ=60)

                                                                                                    GGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGA                                                 <  SRR19973067.2592171/150‑1 (MQ=60)

                                                                                                     GCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAT                                                <  SRR19973067.1386290/150‑1 (MQ=60)

                                                                                                      CTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATT                                               >  SRR19973067.235080/1‑150 (MQ=60)

                                                                                                       TCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTA                                              >  SRR19973067.374434/1‑150 (MQ=60)

                                                                                                       TCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTA                                              >  SRR19973067.374439/1‑150 (MQ=60)

                                                                                                        CATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAG                                             >  SRR19973067.1608115/1‑150 (MQ=60)

                                                                                                         ATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGT                                            >  SRR19973067.3139976/1‑150 (MQ=60)

                                                                                                           GTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTA                                          <  SRR19973067.1074696/150‑1 (MQ=60)

                                                                                                            TTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAA                                         <  SRR19973067.2740683/150‑1 (MQ=60)

                                                                                                              CCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATG                                       <  SRR19973067.3134158/150‑1 (MQ=60)

                                                                                                              CCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATG                                       <  SRR19973067.3133946/150‑1 (MQ=60)

                                                                                                              CCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATG                                       <  SRR19973067.786043/150‑1 (MQ=60)

                                                                                                              CCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATG                                       <  SRR19973067.785736/150‑1 (MQ=60)

                                                                                                              CCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATACCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATG                                       <  SRR19973067.1229857/150‑1 (MQ=60)

                                                                                                                GTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTC                                     >  SRR19973067.3555221/1‑150 (MQ=60)

                                                                                                                 TCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCA                                    <  SRR19973067.3259010/150‑1 (MQ=60)

                                                                                                                    ATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACC                                 <  SRR19973067.1641810/150‑1 (MQ=60)

                                                                                                                    ATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAACTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACC                                 <  SRR19973067.1641884/150‑1 (MQ=60)

                                                                                                                       CCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGA                              >  SRR19973067.646440/1‑150 (MQ=60)

                                                                                                                       CCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGA                              >  SRR19973067.646639/1‑150 (MQ=60)

                                                                                                                         GGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGT                            >  SRR19973067.1615340/1‑150 (MQ=60)

                                                                                                                           AGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGAGTTGTATGGCATAAACCTCTTGGGGCCAGAACGGCAGAACATGCAATTCCAGTCAGGTGTAACACCGGAAACGCGGGATTAGTTAAAGTCAACCTTAGTCT                          >  SRR19973067.823356/1‑150 (MQ=60)

                                                                                                                              GACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAC                       >  SRR19973067.2826064/1‑150 (MQ=60)

                                                                                                                              GACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAC                       >  SRR19973067.2825396/1‑150 (MQ=60)

                                                                                                                               ACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACG                      >  SRR19973067.2954628/1‑150 (MQ=60)

                                                                                                                                 AATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGC                    >  SRR19973067.330280/1‑150 (MQ=60)

                                                                                                                                 AATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGC                    >  SRR19973067.851114/1‑150 (MQ=60)

                                                                                                                                  ATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCA                   >  SRR19973067.2684815/1‑150 (MQ=60)

                                                                                                                                   TTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAA                  <  SRR19973067.2394197/150‑1 (MQ=60)

                                                                                                                                    TGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAC                 >  SRR19973067.141038/1‑150 (MQ=60)

                                                                                                                                     GAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACC                >  SRR19973067.804550/1‑150 (MQ=60)

                                                                                                                                     GAAGTTGATTTAGGTTATGTCTTCGCTGCCAATGTCCGTGGTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACC                <  SRR19973067.1968254/150‑1 (MQ=60)

                                                                                                                                      AAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCA               >  SRR19973067.1794448/1‑150 (MQ=60)

                                                                                                                                          TGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGA           >  SRR19973067.3548945/1‑150 (MQ=60)

                                                                                                                                          TGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGA           >  SRR19973067.3548079/1‑150 (MQ=60)

                                                                                                                                           GATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAC          >  SRR19973067.2077614/1‑150 (MQ=60)

                                                                                                                                                AGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAG     <  SRR19973067.1133953/150‑1 (MQ=60)

                                                                                                                                                 GGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGC    >  SRR19973067.1938694/1‑150 (MQ=60)

                                                                                                                                                 GGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGC    >  SRR19973067.1939440/1‑150 (MQ=60)

                                                                                                                                                 GGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGC    >  SRR19973067.128694/1‑150 (MQ=60)

                                                                                                                                                  GTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCT   >  SRR19973067.232080/1‑150 (MQ=60)

                                                                                                                                                   TTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTA  >  SRR19973067.2691556/1‑150 (MQ=60)

GCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTA  >  CP009273/997817‑998113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`