Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 997,965 G→T 92.1% D235Y (GAT→TAT)  elfG → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273997,9650GT92.1% 750.6 / 51.7 257D235Y (GAT→TAT) elfGputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (13/8);  new base T (124/112);  total (137/120)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGC  >  CP009273/997816‑998111
                                                                                                                                                     |                                                                                                                                                  
  cgATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTAt                                                                                                                                                  <  2:2642382/150‑1 (MQ=255)
  cgATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGAt                                                                                                                                                  >  2:4347202/1‑150 (MQ=255)
  cgATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGAACACCGGATTTAGTGTATTACCTGTCTGGAAAGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAAATGAAGTTGATTTAGGTTAt                                                                                                                                                  >  1:2396606/1‑150 (MQ=255)
   gATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATg                                                                                                                                                 >  1:4232346/1‑150 (MQ=255)
    aTTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGt                                                                                                                                                <  1:4331335/150‑1 (MQ=255)
     ttATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTc                                                                                                                                               <  1:2079050/150‑1 (MQ=255)
       atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                             <  2:2977082/150‑1 (MQ=255)
       atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                             >  1:508016/1‑150 (MQ=255)
       atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                             <  2:3692820/150‑1 (MQ=255)
       atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCtt                                                                                                                                             >  1:3666683/1‑150 (MQ=255)
        tatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTc                                                                                                                                            <  2:1314036/150‑1 (MQ=255)
         atGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCg                                                                                                                                           <  2:1624893/150‑1 (MQ=255)
           gAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCt                                                                                                                                         >  1:407633/1‑150 (MQ=255)
             aTGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGc                                                                                                                                       <  2:1990143/150‑1 (MQ=255)
                 tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAt                                                                                                                                   <  1:2262312/150‑1 (MQ=255)
                 tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAt                                                                                                                                   >  2:1477928/1‑150 (MQ=255)
                 tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAt                                                                                                                                   <  1:3111534/150‑1 (MQ=255)
                    aacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTc                                                                                                                                <  1:3937508/150‑1 (MQ=255)
                    aacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTc                                                                                                                                <  1:3935091/150‑1 (MQ=255)
                    aacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTc                                                                                                                                >  1:1318712/1‑150 (MQ=255)
                      caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCg                                                                                                                              >  2:4193203/1‑150 (MQ=255)
                       aTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCgt                                                                                                                             >  1:193655/1‑150 (MQ=255)
                           ccGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGtgttg                                                                                                                         >  2:4424387/1‑150 (MQ=255)
                           ccGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGtgttg                                                                                                                         <  2:1754309/150‑1 (MQ=255)
                             gCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTa                                                                                                                       <  1:1394686/150‑1 (MQ=255)
                              cAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAg                                                                                                                      >  1:2685900/1‑150 (MQ=255)
                               aGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAgg                                                                                                                     >  2:1367341/1‑150 (MQ=255)
                                ggTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAggg                                                                                                                    <  1:2721973/150‑1 (MQ=255)
                                 gTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGc                                                                                                                   >  2:1771220/1‑150 (MQ=255)
                                 gTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGc                                                                                                                   >  1:2873951/1‑150 (MQ=255)
                                     ttCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                                                                                                               >  1:321488/1‑150 (MQ=255)
                                      tCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaaa                                                                                                              <  2:4218416/150‑1 (MQ=255)
                                       ccTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAc                                                                                                             <  2:792284/150‑1 (MQ=255)
                                         tGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACct                                                                                                           <  2:2800851/150‑1 (MQ=255)
                                          gCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACctc                                                                                                          <  1:3813683/150‑1 (MQ=255)
                                            aCGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCtt                                                                                                        >  1:1815185/1‑150 (MQ=255)
                                              gACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTgg                                                                                                      <  1:312708/150‑1 (MQ=255)
                                               aCTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTggg                                                                                                     <  1:938356/150‑1 (MQ=255)
                                               aCTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTggg                                                                                                     <  2:1950644/150‑1 (MQ=255)
                                                  aCAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGcc                                                                                                  <  1:1960731/150‑1 (MQ=255)
                                                   cAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCa                                                                                                 <  1:817852/150‑1 (MQ=255)
                                                    aGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAg                                                                                                >  1:4593768/1‑150 (MQ=255)
                                                       tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                             <  2:845398/150‑1 (MQ=255)
                                                         ctccGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                           <  2:2371052/148‑1 (MQ=255)
                                                         cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                           >  2:2481685/1‑150 (MQ=255)
                                                         cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                           >  1:4625970/1‑150 (MQ=255)
                                                          acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                          >  2:938051/1‑150 (MQ=255)
                                                          acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                          >  2:3567405/1‑150 (MQ=255)
                                                             ggTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGa                                                                                       <  1:3303860/150‑1 (MQ=255)
                                                             ggTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGa                                                                                       >  1:1983546/1‑150 (MQ=255)
                                                              gTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGaa                                                                                      >  1:4114427/1‑150 (MQ=255)
                                                              gTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGaa                                                                                      >  2:4034472/1‑150 (MQ=255)
                                                               ttttAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAAc                                                                                     <  2:617183/150‑1 (MQ=255)
                                                                    gtgtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCa                                                                                <  2:3673213/150‑1 (MQ=255)
                                                                    gtgtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCa                                                                                <  2:3989538/150‑1 (MQ=255)
                                                                      gtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt                                                                              <  2:3338429/150‑1 (MQ=255)
                                                                        aTTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTc                                                                            >  1:1735867/1‑150 (MQ=255)
                                                                         ttACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc                                                                           <  2:3116243/150‑1 (MQ=255)
                                                                         ttACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc                                                                           <  1:376533/150‑1 (MQ=255)
                                                                             cTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc                                                                       >  1:2107783/1‑150 (MQ=255)
                                                                              tGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCa                                                                      >  2:2848298/1‑150 (MQ=255)
                                                                               gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAg                                                                     >  2:238809/1‑150 (MQ=255)
                                                                                tCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAgg                                                                    >  2:2252490/1‑150 (MQ=255)
                                                                                 cTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgt                                                                   <  1:1678499/150‑1 (MQ=255)
                                                                                  tGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtg                                                                  >  1:360241/1‑150 (MQ=255)
                                                                                  tGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtg                                                                  <  2:2559344/150‑1 (MQ=255)
                                                                                        gATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAacac                                                            >  2:1102439/1‑150 (MQ=255)
                                                                                         aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACg                                                           <  2:1217346/150‑1 (MQ=255)
                                                                                         aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACg                                                           <  2:2156841/150‑1 (MQ=255)
                                                                                           cAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACggg                                                         >  1:1227223/1‑150 (MQ=255)
                                                                                            aaTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAAGTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGa                                                        >  1:2937734/1‑150 (MQ=255)
                                                                                             aTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaa                                                       <  1:21923/150‑1 (MQ=255)
                                                                                               tCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAc                                                     >  2:875177/1‑150 (MQ=255)
                                                                                                  cTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgcg                                                  <  2:2071754/150‑1 (MQ=255)
                                                                                                  cTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgcg                                                  <  2:685642/150‑1 (MQ=255)
                                                                                                   ttGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCgg                                                 <  2:328830/150‑1 (MQ=255)
                                                                                                     ggCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGa                                               <  2:182219/150‑1 (MQ=255)
                                                                                                        tCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTa                                            <  2:2391359/150‑1 (MQ=255)
                                                                                                        tCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTa                                            <  2:4322079/150‑1 (MQ=255)
                                                                                                         cATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAg                                           <  2:1240432/150‑1 (MQ=255)
                                                                                                          aTGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGt                                          <  1:863308/150‑1 (MQ=255)
                                                                                                          aTGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGt                                          <  2:272522/150‑1 (MQ=255)
                                                                                                           tggtCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGtt                                         <  1:3544030/147‑1 (MQ=255)
                                                                                                                            agagaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTTCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCt                        >  1:2795014/1‑150 (MQ=255)
                                                                                                                            agagaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCt                        >  1:1022974/1‑150 (MQ=255)
                                                                                                                              agaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGa                      <  2:3814008/150‑1 (MQ=255)
                                                                                                                               gaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAc                     >  2:4282638/1‑150 (MQ=255)
                                                                                                                               gaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAc                     <  1:4204525/150‑1 (MQ=255)
                                                                                                                               gaCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAc                     >  2:1142998/1‑150 (MQ=255)
                                                                                                                                 cAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg                   >  1:3757667/1‑150 (MQ=255)
                                                                                                                                   aTTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCa                 >  1:728254/1‑150 (MQ=255)
                                                                                                                                   aTTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCa                 <  2:1863029/150‑1 (MQ=255)
                                                                                                                                     tGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAc               >  2:3807970/1‑150 (MQ=255)
                                                                                                                                     tGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAc               >  2:4552916/1‑150 (MQ=255)
                                                                                                                                      gAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAcc              >  1:2556055/1‑150 (MQ=255)
                                                                                                                                       aaGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACca             <  2:3140058/150‑1 (MQ=255)
                                                                                                                                            gATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAc        >  1:283548/1‑150 (MQ=255)
                                                                                                                                             aTTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcc       >  2:1485970/1‑150 (MQ=255)
                                                                                                                                                tAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa    >  1:4573914/1‑150 (MQ=255)
                                                                                                                                                 aGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAg   <  1:163966/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGC  >  CP009273/997816‑998111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAG  >  CP009273/997816‑998110
                                                                                                                                                     |                                                                                                                                                 
CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTT                                                                                                                                                   <  SRR19973073.3137541/150‑1 (MQ=60)
  CGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGAACACCGGATTTAGTGTATTACCTGTCTGGAAAGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAAATGAAGTTGATTTAGGTTAT                                                                                                                                                 >  SRR19973073.2401528/1‑150 (MQ=60)
   GATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATG                                                                                                                                                >  SRR19973073.4240729/1‑150 (MQ=60)
    ATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGT                                                                                                                                               <  SRR19973073.910894/150‑1 (MQ=60)
    ATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGT                                                                                                                                               <  SRR19973073.4339991/150‑1 (MQ=60)
     TTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTC                                                                                                                                              <  SRR19973073.2083391/150‑1 (MQ=60)
       ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                            >  SRR19973073.509107/1‑150 (MQ=60)
       ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTT                                                                                                                                            <  SRR19973073.2064892/150‑1 (MQ=60)
       ATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTT                                                                                                                                            >  SRR19973073.3674036/1‑150 (MQ=60)
           GAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCT                                                                                                                                        >  SRR19973073.408446/1‑150 (MQ=60)
                 TACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAT                                                                                                                                  <  SRR19973073.2835046/150‑1 (MQ=60)
                 TACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAT                                                                                                                                  <  SRR19973073.3117769/150‑1 (MQ=60)
                 TACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAT                                                                                                                                  <  SRR19973073.3117790/150‑1 (MQ=60)
                 TACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAT                                                                                                                                  <  SRR19973073.2266950/150‑1 (MQ=60)
                 TACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAT                                                                                                                                  <  SRR19973073.3117858/150‑1 (MQ=60)
                 TACAACAGACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAAT                                                                                                                                  <  SRR19973073.3117781/150‑1 (MQ=60)
                    AACATACCCGCAGGTGATTCCTTCACGACTACATGTACACCGGTTTTAGTGTATTACCTGTCTTGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               <  SRR19973073.1819563/150‑1 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               <  SRR19973073.3942984/150‑1 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               >  SRR19973073.1321357/1‑150 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               >  SRR19973073.1321506/1‑150 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               <  SRR19973073.3945354/150‑1 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               <  SRR19973073.3945378/150‑1 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               <  SRR19973073.3942908/150‑1 (MQ=60)
                    AACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTC                                                                                                                               <  SRR19973073.3942929/150‑1 (MQ=60)
                     ACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCC                                                                                                                              >  SRR19973073.1950420/1‑150 (MQ=60)
                     ACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCC                                                                                                                              >  SRR19973073.2492392/1‑150 (MQ=60)
                       ATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGT                                                                                                                            >  SRR19973073.194111/1‑150 (MQ=60)
                        TACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTG                                                                                                                           >  SRR19973073.2742827/1‑150 (MQ=60)
                        TACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGAATTAGGTTATGTCTTCGCTGCCAATTTCCGTG                                                                                                                           >  SRR19973073.4259888/1‑150 (MQ=60)
                             GCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTA                                                                                                                      >  SRR19973073.4229747/1‑150 (MQ=60)
                             GCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTA                                                                                                                      <  SRR19973073.1397637/150‑1 (MQ=60)
                             GCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCAGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTTGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTA                                                                                                                      >  SRR19973073.4229053/1‑150 (MQ=60)
                              CAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAG                                                                                                                     >  SRR19973073.2691389/1‑150 (MQ=60)
                              CAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGACTTCGCTGCCAATTTCCGTGTTGTAG                                                                                                                     >  SRR19973073.2691422/1‑150 (MQ=60)
                                GGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGG                                                                                                                   <  SRR19973073.2727533/150‑1 (MQ=60)
                                GGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGG                                                                                                                   <  SRR19973073.2727521/150‑1 (MQ=60)
                                GGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGG                                                                                                                   >  SRR19973073.2370354/1‑150 (MQ=60)
                                 GTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGC                                                                                                                  >  SRR19973073.2879824/1‑150 (MQ=60)
                                    ATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATA                                                                                                               >  SRR19973073.1655553/1‑150 (MQ=60)
                                     TTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAA                                                                                                              >  SRR19973073.1596292/1‑150 (MQ=60)
                                     TTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAA                                                                                                              >  SRR19973073.322150/1‑150 (MQ=60)
                                        CTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACC                                                                                                           <  SRR19973073.1964190/150‑1 (MQ=60)
                                         TGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCT                                                                                                          >  SRR19973073.1803469/1‑150 (MQ=60)
                                          GCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTC                                                                                                         <  SRR19973073.3821286/150‑1 (MQ=60)
                                            ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTT                                                                                                       >  SRR19973073.1818938/1‑150 (MQ=60)
                                             CGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTG                                                                                                      <  SRR19973073.2231856/150‑1 (MQ=60)
                                             CGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTG                                                                                                      <  SRR19973073.2231828/150‑1 (MQ=60)
                                             CGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTG                                                                                                      <  SRR19973073.2107395/150‑1 (MQ=60)
                                              GACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGG                                                                                                     <  SRR19973073.313357/150‑1 (MQ=60)
                                               ACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGG                                                                                                    <  SRR19973073.940310/150‑1 (MQ=60)
                                                CTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGG                                                                                                   >  SRR19973073.2898157/1‑150 (MQ=60)
                                                 TACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGC                                                                                                  >  SRR19973073.2763030/1‑150 (MQ=60)
                                                  ACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCC                                                                                                 <  SRR19973073.3279997/150‑1 (MQ=60)
                                                  ACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCC                                                                                                 <  SRR19973073.1964770/150‑1 (MQ=60)
                                                   CAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCA                                                                                                <  SRR19973073.819522/150‑1 (MQ=60)
                                                    AGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCCTAAACCTCTTGGGGCCAG                                                                                               >  SRR19973073.615848/1‑150 (MQ=60)
                                                    AGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAG                                                                                               >  SRR19973073.4602948/1‑150 (MQ=60)
                                                    AGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAG                                                                                               >  SRR19973073.615860/1‑150 (MQ=60)
                                                    AGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAG                                                                                               >  SRR19973073.4080389/1‑150 (MQ=60)
                                                        ACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACG                                                                                           >  SRR19973073.4625765/1‑150 (MQ=60)
                                                         CACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGG                                                                                          >  SRR19973073.4635163/1‑150 (MQ=60)
                                                         CACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGG                                                                                          >  SRR19973073.4635196/1‑150 (MQ=60)
                                                            CGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAG                                                                                       >  SRR19973073.1757075/1‑150 (MQ=60)
                                                             GGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGA                                                                                      <  SRR19973073.3310496/150‑1 (MQ=60)
                                                             GGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGA                                                                                      >  SRR19973073.1694044/1‑150 (MQ=60)
                                                             GGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGA                                                                                      <  SRR19973073.3310547/150‑1 (MQ=60)
                                                             GGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGA                                                                                      >  SRR19973073.1987655/1‑150 (MQ=60)
                                                              GTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAA                                                                                     >  SRR19973073.4122660/1‑150 (MQ=60)
                                                              GTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAA                                                                                     >  SRR19973073.4122671/1‑150 (MQ=60)
                                                                   AGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGC                                                                                >  SRR19973073.2159656/1‑150 (MQ=60)
                                                                      GTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAT                                                                             >  SRR19973073.3206513/1‑150 (MQ=60)
                                                                        ATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTC                                                                           >  SRR19973073.1739475/1‑150 (MQ=60)
                                                                         TTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCC                                                                          <  SRR19973073.377271/150‑1 (MQ=60)
                                                                         TTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCC                                                                          <  SRR19973073.1736586/150‑1 (MQ=60)
                                                                             CTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTC                                                                      >  SRR19973073.2112194/1‑150 (MQ=60)
                                                                                 CTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGT                                                                  <  SRR19973073.1681987/150‑1 (MQ=60)
                                                                                 CTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGT                                                                  >  SRR19973073.4354685/1‑150 (MQ=60)
                                                                                  TGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTG                                                                 >  SRR19973073.360962/1‑150 (MQ=60)
                                                                                  TGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTG                                                                 >  SRR19973073.360905/1‑150 (MQ=60)
                                                                                   GGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGT                                                                <  SRR19973073.2664155/150‑1 (MQ=60)
                                                                                      ACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAAC                                                             <  SRR19973073.1393828/150‑1 (MQ=60)
                                                                                       CGTTCTATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACA                                                            <  SRR19973073.2864939/150‑1 (MQ=60)
                                                                                        GATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACAC                                                           >  SRR19973073.3165562/1‑150 (MQ=60)
                                                                                         ATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTAGCTGCCAATTTCCGTTTTGTAGGGCATAAACCAATTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACG                                                          >  SRR19973073.3711735/1‑150 (MQ=60)
                                                                                           CAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGG                                                        >  SRR19973073.1229786/1‑150 (MQ=60)
                                                                                            AATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAAGTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGA                                                       >  SRR19973073.2943738/1‑150 (MQ=60)
                                                                                             ATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAA                                                      <  SRR19973073.21991/150‑1 (MQ=60)
                                                                                               TCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAC                                                    <  SRR19973073.4469222/150‑1 (MQ=60)
                                                                                                  CTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCG                                                 <  SRR19973073.913302/150‑1 (MQ=60)
                                                                                                       CTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATT                                            >  SRR19973073.2061927/1‑150 (MQ=60)
                                                                                                         CATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAG                                          >  SRR19973073.3815711/1‑150 (MQ=60)
                                                                                                          ATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGT                                         <  SRR19973073.865099/150‑1 (MQ=60)
                                                                                                           TGGTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTT                                        <  SRR19973073.3551129/150‑1 (MQ=60)
                                                                                                            GTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTA                                       >  SRR19973073.415132/1‑150 (MQ=60)
                                                                                                            GTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTA                                       <  SRR19973073.286876/150‑1 (MQ=60)
                                                                                                              TCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAAT                                     >  SRR19973073.3826870/1‑150 (MQ=60)
                                                                                                              TCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAAT                                     >  SRR19973073.3596022/1‑150 (MQ=60)
                                                                                                               CCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATG                                    >  SRR19973073.2523490/1‑150 (MQ=60)
                                                                                                                    AATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAC                               >  SRR19973073.3637652/1‑150 (MQ=60)
                                                                                                                    AATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAC                               >  SRR19973073.851008/1‑150 (MQ=60)
                                                                                                                           GAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTC                        >  SRR19973073.1202372/1‑150 (MQ=60)
                                                                                                                            AGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTTCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCT                       >  SRR19973073.2800743/1‑150 (MQ=60)
                                                                                                                            AGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCT                       >  SRR19973073.1025113/1‑150 (MQ=60)
                                                                                                                               GACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAC                    <  SRR19973073.4212873/150‑1 (MQ=60)
                                                                                                                               GACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGTGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGAC                    >  SRR19973073.811771/1‑150 (MQ=60)
                                                                                                                                ACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCCATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACG                   <  SRR19973073.1972428/150‑1 (MQ=60)
                                                                                                                                ACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACG                   >  SRR19973073.3765112/1‑150 (MQ=60)
                                                                                                                                 CAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGG                  <  SRR19973073.3447350/150‑1 (MQ=60)
                                                                                                                                 CAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGG                  >  SRR19973073.468481/1‑150 (MQ=60)
                                                                                                                                 CAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGG                  >  SRR19973073.3765220/1‑150 (MQ=60)
                                                                                                                                 CAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGG                  >  SRR19973073.3765197/1‑150 (MQ=60)
                                                                                                                                  AATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGC                 >  SRR19973073.378087/1‑150 (MQ=60)
                                                                                                                                   ATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCA                >  SRR19973073.729746/1‑150 (MQ=60)
                                                                                                                                    TTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCGA               <  SRR19973073.2176101/150‑1 (MQ=60)
                                                                                                                                    TTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAA               >  SRR19973073.1444741/1‑150 (MQ=60)
                                                                                                                                    TTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAA               <  SRR19973073.8024/150‑1 (MQ=60)
                                                                                                                                      GAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACC             >  SRR19973073.2561328/1‑150 (MQ=60)
                                                                                                                                            GATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAC       >  SRR19973073.284151/1‑150 (MQ=60)
                                                                                                                                              TTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCC     >  SRR19973073.1305710/1‑150 (MQ=60)
                                                                                                                                                TAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCA   <  SRR19973073.4089886/150‑1 (MQ=60)
                                                                                                                                                TAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCA   >  SRR19973073.4583066/1‑150 (MQ=60)
                                                                                                                                                TAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCA   >  SRR19973073.4583061/1‑150 (MQ=60)
                                                                                                                                                 AGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAG  <  SRR19973073.164360/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
CGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAG  >  CP009273/997816‑998110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑