Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F181 I0 R1	1262	323.1	10297870	97.5%	10040423	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	997,965	G→T	37.8%	D235Y (GAT→TAT)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	997,965	0	G	T	37.8%	396.5 / inf	291	D235Y (GAT→TAT)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand): ref base G (96/85); new base T (62/48); total (158/133)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

CGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTAT  >  CP009273/997818‑998114

cgATTATATGCATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACCGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTAt                                                                                                                                                     <  2:668669/150‑1 (MQ=255)

 gATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATg                                                                                                                                                    <  1:1517417/150‑1 (MQ=255)

 gATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATg                                                                                                                                                    <  1:2639804/150‑1 (MQ=255)

   ttATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTGACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGATGTTGATTTAGGTTATGTc                                                                                                                                                  <  1:478544/150‑1 (MQ=255)

   ttATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTc                                                                                                                                                  >  1:3343860/1‑150 (MQ=255)

     atatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCtt                                                                                                                                                >  1:4195833/1‑150 (MQ=255)

      tatGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTc                                                                                                                                               <  1:68287/150‑1 (MQ=255)

          aaTGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTg                                                                                                                                           <  2:4685190/150‑1 (MQ=255)

            tGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGcc                                                                                                                                         <  1:2562247/150‑1 (MQ=255)

               tACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAAt                                                                                                                                      >  2:1800060/1‑150 (MQ=255)

                  aacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTc                                                                                                                                   >  1:4065549/1‑150 (MQ=255)

                    caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCg                                                                                                                                 <  2:3299027/150‑1 (MQ=255)

                    caTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCg                                                                                                                                 >  1:4581967/1‑150 (MQ=255)

                     aTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCgt                                                                                                                                >  2:1687929/1‑150 (MQ=255)

                      tACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCgtg                                                                                                                               <  2:774647/150‑1 (MQ=255)

                         ccGCAGGTGAGTCCTGCACGACTACCGGGACACCGGTTTGAGTGTATTACCTGTCTGGTACGATCAATTCACGTGGCGCATGTTCCGTCAAGGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATGTCCGtgttg                                                                                                                            <  2:2542980/150‑1 (MQ=255)

                          cGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTTCGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttgt                                                                                                                           <  2:2433060/150‑1 (MQ=255)

                          cGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttgt                                                                                                                           <  1:3778713/150‑1 (MQ=255)

                          cGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGtgttgt                                                                                                                           >  2:4988258/1‑150 (MQ=255)

                            cAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAg                                                                                                                         <  1:4543788/150‑1 (MQ=255)

                                tGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCa                                                                                                                     >  1:2721677/1‑150 (MQ=255)

                                 gATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAAGTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCAt                                                                                                                    >  2:4800565/1‑150 (MQ=255)

                                  aTTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATa                                                                                                                   <  2:1134311/150‑1 (MQ=255)

                                   ttCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                                                                                                                  >  1:3014361/1‑150 (MQ=255)

                                      cTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAcc                                                                                                               >  2:2397072/1‑150 (MQ=255)

                                      cTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTGAGGTGATGTCTTCGCTGCCAAATTCCGTGTTGTAGGGCATACAcc                                                                                                               >  2:4583895/1‑150 (MQ=255)

                                         cACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACctct                                                                                                            >  2:2625271/1‑150 (MQ=255)

                                          aCGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCtt                                                                                                           >  2:3733536/1‑150 (MQ=255)

                                            gACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTgg                                                                                                         <  2:568829/150‑1 (MQ=255)

                                             aCTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTggg                                                                                                        <  1:1336094/150‑1 (MQ=255)

                                              cTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTgggg                                                                                                       <  1:4405195/150‑1 (MQ=255)

                                                aCAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGcc                                                                                                     >  1:3990686/1‑150 (MQ=255)

                                                 cAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCa                                                                                                    <  2:428482/150‑1 (MQ=255)

                                                    gTACACCGGTTTTAGTGTATTACCTGTCTTGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGaa                                                                                                 <  1:4882872/150‑1 (MQ=255)

                                                    gTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGaa                                                                                                 <  1:2340034/150‑1 (MQ=255)

                                                     tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                                >  1:3673063/1‑150 (MQ=255)

                                                     tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                                <  2:1468145/150‑1 (MQ=255)

                                                     tACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAAc                                                                                                <  1:1800060/150‑1 (MQ=255)

                                                       cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACACTTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                              >  1:2861688/1‑150 (MQ=255)

                                                       cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACgg                                                                                              >  1:1616732/1‑150 (MQ=255)

                                                                 aGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc                                                                                    <  2:177821/150‑1 (MQ=255)

                                                                 aGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc                                                                                    >  2:5072613/1‑150 (MQ=255)

                                                                   tgtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCaa                                                                                  >  1:282576/1‑150 (MQ=255)

                                                                    gtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt                                                                                 <  1:1411204/150‑1 (MQ=255)

                                                                        tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCa                                                                             <  1:2114292/150‑1 (MQ=255)

                                                                         tccTGTCTGGTACGATCAATTCACTTGGCTCATGTTCGGTCAATGCCGGAGAGACTATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCTATTCAAg                                                                            <  2:3332097/149‑1 (MQ=255)

                                                                         aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAg                                                                            <  2:210968/150‑1 (MQ=255)

                                                                         aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAg                                                                            >  2:3193350/1‑150 (MQ=255)

                                                                          ccTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt                                                                           >  2:1853370/1‑150 (MQ=255)

                                                                                tGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtg                                                                     >  1:2060695/1‑150 (MQ=255)

                                                                                   tACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTaa                                                                  >  1:4747937/1‑150 (MQ=255)

                                                                                   tACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTaa                                                                  <  2:1299071/150‑1 (MQ=255)

                                                                                      gATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAacac                                                               >  1:298615/1‑150 (MQ=255)

                                                                                      gATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAacac                                                               <  1:217309/150‑1 (MQ=255)

                                                                                       aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACg                                                              >  1:3848317/1‑150 (MQ=255)

                                                                                        tCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACgg                                                             >  1:1860884/1‑150 (MQ=255)

                                                                                        tCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACgg                                                             >  1:2369504/1‑150 (MQ=255)

                                                                                         cAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACggg                                                            <  2:3674777/150‑1 (MQ=255)

                                                                                          aaTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGa                                                           >  1:2943258/1‑150 (MQ=255)

                                                                                           aTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaa                                                          >  1:239859/1‑150 (MQ=255)

                                                                                            ttCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaaa                                                         <  1:4494228/150‑1 (MQ=255)

                                                                                              cACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcg                                                       <  1:3770034/150‑1 (MQ=255)

                                                                                              cACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcg                                                       <  1:4709811/150‑1 (MQ=255)

                                                                                                cTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAAcgcg                                                     >  1:1440497/1‑150 (MQ=255)

                                                                                                  tGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCggg                                                   >  2:1596888/1‑150 (MQ=255)

                                                                                                  tGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCggg                                                   <  2:3885906/150‑1 (MQ=255)

                                                                                                  tGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCggg                                                   >  1:754422/1‑150 (MQ=255)

                                                                                                     cTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAtt                                                >  1:3111989/1‑150 (MQ=255)

                                                                                                      tCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTa                                               <  1:1164105/150‑1 (MQ=255)

                                                                                                        aTGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGt                                             >  2:3068346/1‑150 (MQ=255)

                                                                                                          gTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTa                                           >  2:2725448/1‑150 (MQ=255)

                                                                                                           ttCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTaa                                          <  2:4033011/150‑1 (MQ=255)

                                                                                                           ttCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTaa                                          >  1:431363/1‑150 (MQ=255)

                                                                                                              cGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGt                                       >  2:2861509/1‑150 (MQ=255)

                                                                                                                tCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCa                                     >  2:3412219/1‑150 (MQ=255)

                                                                                                                tCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCa                                     >  2:4460132/1‑150 (MQ=255)

                                                                                                                  aaTGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAAc                                   >  1:193417/1‑150 (MQ=255)

                                                                                                                  aaTGCCGGAGAGACAAGTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTACCGTGTTGTAGGGCATAAACCTCTTGGGGACAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGCGAAACGCGGGAGTAGTTAATGTCAAc                                   >  2:4078355/1‑150 (MQ=255)

                                                                                                                    tGCCGGAGAGACAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCt                                 <  1:1127369/150‑1 (MQ=255)

                                                                                                                     gCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTg                                >  2:175131/1‑150 (MQ=255)

                                                                                                                        ggAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGt                             >  1:198185/1‑150 (MQ=255)

                                                                                                                        ggAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGt                             >  2:2764467/1‑150 (MQ=255)

                                                                                                                          agagaCAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCt                           <  2:2314038/150‑1 (MQ=255)

                                                                                                                          agagaCAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCt                           >  2:4368747/1‑150 (MQ=255)

                                                                                                                          agagaCAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCt                           >  2:443077/1‑150 (MQ=255)

                                                                                                                              aCAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACg                       >  2:4835824/1‑150 (MQ=255)

                                                                                                                               cAATTGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg                      >  1:1471871/1‑150 (MQ=255)

                                                                                                                                  ttGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCaa                   >  1:1032347/1‑150 (MQ=255)

                                                                                                                                   tGAAGTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAAc                  >  1:3818835/1‑150 (MQ=255)

                                                                                                                                       gTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcaca              >  1:3326317/1‑150 (MQ=255)

                                                                                                                                       gTTGATTTAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcaca              >  2:389581/1‑150 (MQ=255)

                                                                                                                                       gTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTACCACGCGACACGCCCGCTTAGATAATGTCAACCTGAGTCTGACGGCAACcaca              >  2:4140931/1‑150 (MQ=255)

                                                                                                                                              tAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa       <  1:4312821/150‑1 (MQ=255)

                                                                                                                                              tAGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa       <  1:812822/150‑1 (MQ=255)

                                                                                                                                              tAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa       >  1:358868/1‑150 (MQ=255)

                                                                                                                                              tAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCa       >  2:2938683/1‑150 (MQ=255)

                                                                                                                                               aGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAg      >  2:3868663/1‑150 (MQ=255)

                                                                                                                                               aGGTTATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAg      >  1:5010545/1‑150 (MQ=255)

                                                                                                                                               aGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAg      >  2:2025212/1‑150 (MQ=255)

                                                                                                                                                   gATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGGTAt  <  2:4372588/150‑1 (MQ=255)

CGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTAT  >  CP009273/997818‑998114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A