Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,049,407	G→T	86.7%	T743K (ACG→AAG)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,049,407	0	G	T	86.7%	669.9 / 94.9	247	T743K (ACG→AAG)	torS	hybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand): ref base G (17/16); new base T (106/108); total (123/124)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

GACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  >  CP009273/1049261‑1049555

 aCCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAAt                                                                                                                                                  <  2:355604/150‑1 (MQ=255)

  ccGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATg                                                                                                                                                 <  1:3065140/150‑1 (MQ=255)

   cGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGc                                                                                                                                                >  1:4119343/1‑150 (MQ=255)

   cGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGc                                                                                                                                                >  1:3891655/1‑150 (MQ=255)

    gAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGcc                                                                                                                                               >  2:2127481/1‑150 (MQ=255)

       cACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTc                                                                                                                                            <  2:3613178/150‑1 (MQ=255)

         cTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGa                                                                                                                                          <  1:2228160/150‑1 (MQ=255)

          ttCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGat                                                                                                                                         >  2:2686543/1‑150 (MQ=255)

           tCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGata                                                                                                                                        >  2:286651/1‑150 (MQ=255)

           tCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGata                                                                                                                                        <  2:4230372/150‑1 (MQ=255)

             aCGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATc                                                                                                                                      <  2:4416900/150‑1 (MQ=255)

              cgcgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATcc                                                                                                                                     >  1:2956581/1‑150 (MQ=255)

                 ggCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGc                                                                                                                                  >  1:1000723/1‑150 (MQ=255)

                   cACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAg                                                                                                                                <  1:318586/150‑1 (MQ=255)

                   cACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAg                                                                                                                                >  2:1317102/1‑150 (MQ=255)

                   cACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTAAATGCCGTCGATATCCGGCAg                                                                                                                                >  1:3662284/1‑150 (MQ=255)

                       ggTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCa                                                                                                                            >  2:4016673/1‑150 (MQ=255)

                        gTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaa                                                                                                                           <  1:417749/150‑1 (MQ=255)

                        gTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaa                                                                                                                           <  1:2289383/150‑1 (MQ=255)

                        gTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaa                                                                                                                           >  2:763350/1‑150 (MQ=255)

                        gTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaa                                                                                                                           <  2:3169350/150‑1 (MQ=255)

                             gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATcc                                                                                                                      <  1:2621143/150‑1 (MQ=255)

                             gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATcc                                                                                                                      <  1:354864/150‑1 (MQ=255)

                                aTAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATccacc                                                                                                                   >  2:1197567/1‑150 (MQ=255)

                                  aaTCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAg                                                                                                                 >  1:4273594/1‑150 (MQ=255)

                                   aTCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGt                                                                                                                >  2:1730150/1‑150 (MQ=255)

                                    tCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTg                                                                                                               <  2:1113345/150‑1 (MQ=255)

                                     cccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGc                                                                                                              <  1:1968442/150‑1 (MQ=255)

                                     cccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGc                                                                                                              <  1:1518441/150‑1 (MQ=255)

                                      ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                             <  2:2264256/150‑1 (MQ=255)

                                       cgcgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCgg                                                                                                            >  1:1973661/1‑150 (MQ=255)

                                        gcgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgc                                                                                                           >  2:714398/1‑150 (MQ=255)

                                              taatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                     <  1:687961/150‑1 (MQ=255)

                                              taatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                     >  1:1911610/1‑150 (MQ=255)

                                               aatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAAc                                                                                                    >  2:713625/1‑150 (MQ=255)

                                               aatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAAc                                                                                                    <  2:2616047/150‑1 (MQ=255)

                                                atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAACCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACg                                                                                                   <  2:3419419/150‑1 (MQ=255)

                                                atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACg                                                                                                   <  1:4480265/150‑1 (MQ=255)

                                                    aCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTc                                                                                               >  2:3298068/1‑150 (MQ=255)

                                                       ggTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCt                                                                                            >  2:36661/1‑150 (MQ=255)

                                                        ttACGCTGGCGCTGCTTTTCGTCAATTACTTGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCTATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTa                                                                                           <  1:3498086/149‑1 (MQ=255)

                                                        gTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTa                                                                                           >  2:4391184/1‑150 (MQ=255)

                                                             cTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTg                                                                                      >  1:2401181/1‑150 (MQ=255)

                                                              tGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGc                                                                                     >  1:835774/1‑150 (MQ=255)

                                                                  gcAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAgtg                                                                                 <  2:355296/150‑1 (MQ=255)

                                                                  gcAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCCGTGCGGCAGCAAACGGTTCGCTATTTTGCAgtg                                                                                 <  1:1283819/150‑1 (MQ=255)

                                                                     gCGTTTCGACAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGtct                                                                              <  2:1188207/150‑1 (MQ=255)

                                                                       gTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTctct                                                                            >  2:2857098/1‑150 (MQ=255)

                                                                       gTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTctct                                                                            >  2:3969508/1‑150 (MQ=255)

                                                                         ttCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTaa                                                                          >  2:371846/1‑150 (MQ=255)

                                                                          tCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTaaa                                                                         <  2:621824/150‑1 (MQ=255)

                                                                            gTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGc                                                                       >  2:1971277/1‑150 (MQ=255)

                                                                               aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                    <  2:4206489/150‑1 (MQ=255)

                                                                               aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                    >  2:1573421/1‑150 (MQ=255)

                                                                                aTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgc                                                                   >  1:1894731/1‑150 (MQ=255)

                                                                                 tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                                  <  2:1088788/150‑1 (MQ=255)

                                                                                 tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                                  >  1:4445822/1‑150 (MQ=255)

                                                                                  gACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTCTTTTGCAGTGTCTCTAAAGCCTgcgc                                                                 >  2:1533488/1‑150 (MQ=255)

                                                                                   aCATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGcgcg                                                                <  2:1854920/150‑1 (MQ=255)

                                                                                    cATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCgg                                                               >  1:3471167/1‑150 (MQ=255)

                                                                                     aTGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGc                                                              >  1:2561092/1‑150 (MQ=255)

                                                                                     aTGGGCGCTAAAGCCAAACAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGc                                                              >  1:885742/1‑150 (MQ=255)

                                                                                       gggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAt                                                            <  2:1398651/150‑1 (MQ=255)

                                                                                        ggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAtt                                                           <  1:3973372/150‑1 (MQ=255)

                                                                                           gcTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGcc                                                        >  1:2433988/1‑150 (MQ=255)

                                                                                           gcTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGcc                                                        >  2:2754490/1‑150 (MQ=255)

                                                                                             tAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCaa                                                      >  2:909022/1‑150 (MQ=255)

                                                                                             tAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCaa                                                      >  1:1429445/1‑150 (MQ=255)

                                                                                             tAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCaa                                                      <  1:545562/150‑1 (MQ=255)

                                                                                                aGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAg                                                   <  2:2934398/150‑1 (MQ=255)

                                                                                                 gCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGc                                                  >  2:4110693/1‑150 (MQ=255)

                                                                                                    aaTCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaac                                               >  1:919828/1‑150 (MQ=255)

                                                                                                    aaTCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaac                                               >  1:1597082/1‑150 (MQ=255)

                                                                                                         aaaCCCGCGACGTATATTGCTGTGCCAGTTGTCGTGAAAGATTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCt                                          <  1:199431/150‑1 (MQ=255)

                                                                                                         aaaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCt                                          <  1:3402341/150‑1 (MQ=255)

                                                                                                          aaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTg                                         >  1:2816090/1‑150 (MQ=255)

                                                                                                            ccAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTgcg                                       <  1:3695444/150‑1 (MQ=255)

                                                                                                                cGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacc                                   >  1:3715004/1‑150 (MQ=255)

                                                                                                                 gACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacca                                  >  1:535074/1‑150 (MQ=255)

                                                                                                                 gACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacca                                  <  2:463984/150‑1 (MQ=255)

                                                                                                                 gACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTGCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacca                                  >  1:2082922/1‑150 (MQ=255)

                                                                                                                    ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                               >  2:651885/1‑150 (MQ=255)

                                                                                                                    ggATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                               <  2:1551430/150‑1 (MQ=255)

                                                                                                                      atatTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGt                             <  2:3891655/150‑1 (MQ=255)

                                                                                                                       tatTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGtt                            <  2:3957/150‑1 (MQ=255)

                                                                                                                          tGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTc                         <  1:1764468/150‑1 (MQ=255)

                                                                                                                          tGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTc                         >  1:2707829/1‑150 (MQ=255)

                                                                                                                          tGCTGTGCCAGTTGTCGGGCAAGCTTAAGGCCGTCCATAACCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTc                         <  1:3865175/150‑1 (MQ=255)

                                                                                                                              gtgCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGAAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCa                     <  1:1187900/150‑1 (MQ=255)

                                                                                                                               tgCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAt                    <  1:4184673/150‑1 (MQ=255)

                                                                                                                               tgCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAt                    <  2:80938/150‑1 (MQ=255)

                                                                                                                                   aGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCa                >  1:3709357/1‑150 (MQ=255)

                                                                                                                                     ttGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAt              <  1:2198072/150‑1 (MQ=255)

                                                                                                                                     ttGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAt              <  2:4316077/150‑1 (MQ=255)

                                                                                                                                         cGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTa          <  2:894854/150‑1 (MQ=255)

                                                                                                                                          gggCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTaa         >  2:3593957/1‑150 (MQ=255)

                                                                                                                                          gggCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATATTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACAGGTCTTCAGCAACTCAATGGTaa         >  1:722699/1‑150 (MQ=255)

                                                                                                                                            gCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAAtt       <  1:4460397/150‑1 (MQ=255)

                                                                                                                                                 cGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTg  <  1:2904629/150‑1 (MQ=255)

                                                                                                                                                 cGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTg  <  1:3303519/150‑1 (MQ=255)

GACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  >  CP009273/1049261‑1049555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  >  CP009273/1049258‑1049555

ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCT                                                                                                                                                      <  SRR19973080.3024687/150‑1 (MQ=60)

ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCT                                                                                                                                                      <  SRR19973080.3555504/150‑1 (MQ=60)

   GACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCACGCTTAA                                                                                                                                                   >  SRR19973080.4167879/1‑150 (MQ=60)

   GACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAA                                                                                                                                                   >  SRR19973080.4167900/1‑150 (MQ=60)

     CCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATG                                                                                                                                                 <  SRR19973080.3081810/150‑1 (MQ=60)

      CGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGC                                                                                                                                                >  SRR19973080.4141693/1‑150 (MQ=60)

      CGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGC                                                                                                                                                >  SRR19973080.3913107/1‑150 (MQ=60)

       GAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACTAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCC                                                                                                                                               >  SRR19973080.3603511/1‑150 (MQ=60)

        AGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCG                                                                                                                                              <  SRR19973080.468132/150‑1 (MQ=60)

          CACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCTTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTC                                                                                                                                            <  SRR19973080.3426926/150‑1 (MQ=60)

          CACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTC                                                                                                                                            <  SRR19973080.1965097/150‑1 (MQ=60)

            CTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGA                                                                                                                                          <  SRR19973080.2240597/150‑1 (MQ=60)

            CTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGA                                                                                                                                          <  SRR19973080.2240433/150‑1 (MQ=60)

             TTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGAT                                                                                                                                         <  SRR19973080.396802/150‑1 (MQ=60)

             TTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGAT                                                                                                                                         <  SRR19973080.2672513/150‑1 (MQ=60)

              TCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATA                                                                                                                                        >  SRR19973080.3732554/1‑150 (MQ=60)

               CACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATAT                                                                                                                                       <  SRR19973080.2166550/150‑1 (MQ=60)

                 CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCC                                                                                                                                     <  SRR19973080.996091/150‑1 (MQ=60)

                 CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCC                                                                                                                                     <  SRR19973080.3285272/150‑1 (MQ=60)

                 CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCC                                                                                                                                     >  SRR19973080.2972602/1‑150 (MQ=60)

                 CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCC                                                                                                                                     >  SRR19973080.2972686/1‑150 (MQ=60)

                 CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCC                                                                                                                                     >  SRR19973080.2972809/1‑150 (MQ=60)

                    GGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGC                                                                                                                                  >  SRR19973080.1006721/1‑150 (MQ=60)

                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAG                                                                                                                                <  SRR19973080.320559/150‑1 (MQ=60)

                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAG                                                                                                                                <  SRR19973080.4377535/150‑1 (MQ=60)

                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAG                                                                                                                                >  SRR19973080.4508960/1‑150 (MQ=60)

                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAG                                                                                                                                >  SRR19973080.3682478/1‑150 (MQ=60)

                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTAAATGCCGTCGATATCCGGCAG                                                                                                                                >  SRR19973080.3682473/1‑150 (MQ=60)

                       ACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGA                                                                                                                               >  SRR19973080.2926420/1‑150 (MQ=60)

                           GTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAA                                                                                                                           <  SRR19973080.2301991/150‑1 (MQ=60)

                           GTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAA                                                                                                                           <  SRR19973080.420298/150‑1 (MQ=60)

                             TTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAA                                                                                                                         >  SRR19973080.1351605/1‑150 (MQ=60)

                             TGAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAA                                                                                                                         <  SRR19973080.1528216/150‑1 (MQ=60)

                               AGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATC                                                                                                                       >  SRR19973080.245718/1‑150 (MQ=60)

                                GGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCC                                                                                                                      <  SRR19973080.1535432/150‑1 (MQ=60)

                                GGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCC                                                                                                                      <  SRR19973080.2635344/150‑1 (MQ=60)

                                GGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCC                                                                                                                      <  SRR19973080.356928/150‑1 (MQ=60)

                                   ATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACC                                                                                                                   <  SRR19973080.2915116/150‑1 (MQ=60)

                                    TAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCA                                                                                                                  <  SRR19973080.2409566/150‑1 (MQ=60)

                                     AATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAG                                                                                                                 >  SRR19973080.4296922/1‑150 (MQ=60)

                                     AATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAG                                                                                                                 >  SRR19973080.4296910/1‑150 (MQ=60)

                                        CCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGC                                                                                                              <  SRR19973080.1527147/150‑1 (MQ=60)

                                        CCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGC                                                                                                              <  SRR19973080.2103650/150‑1 (MQ=60)

                                        CCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGC                                                                                                              >  SRR19973080.3767824/1‑150 (MQ=60)

                                        CCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGC                                                                                                              <  SRR19973080.1979206/150‑1 (MQ=60)

                                        CCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGC                                                                                                              <  SRR19973080.1979615/150‑1 (MQ=60)

                                        CCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGC                                                                                                              <  SRR19973080.1979616/150‑1 (MQ=60)

                                         CCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCG                                                                                                             <  SRR19973080.3543987/150‑1 (MQ=60)

                                          CGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGG                                                                                                            >  SRR19973080.1984876/1‑150 (MQ=60)

                                              GAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGC                                                                                                        <  SRR19973080.447670/150‑1 (MQ=60)

                                                 TAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAA                                                                                                     >  SRR19973080.1922366/1‑150 (MQ=60)

                                                 TAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAA                                                                                                     <  SRR19973080.692211/150‑1 (MQ=60)

                                                 TAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAA                                                                                                     <  SRR19973080.692026/150‑1 (MQ=60)

                                                   ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                   <  SRR19973080.3235854/150‑1 (MQ=60)

                                                   ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                   <  SRR19973080.4504511/150‑1 (MQ=60)

                                                   ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCATCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                   <  SRR19973080.4504325/150‑1 (MQ=60)

                                                     GAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGT                                                                                                 <  SRR19973080.834293/150‑1 (MQ=60)

                                                        CTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCG                                                                                              >  SRR19973080.1826562/1‑150 (MQ=60)

                                                        CTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCG                                                                                              >  SRR19973080.1826571/1‑150 (MQ=60)

                                                         TGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGC                                                                                             >  SRR19973080.2013552/1‑150 (MQ=60)

                                                          GGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCCGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCT                                                                                            <  SRR19973080.553915/150‑1 (MQ=60)

                                                           TTACGCTGGCGCTGCTTTTCGTCAATTACTTGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCTATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTA                                                                                           <  SRR19973080.3517376/150‑1 (MQ=60)

                                                                CTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTG                                                                                      >  SRR19973080.2414505/1‑150 (MQ=60)

                                                                CTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTG                                                                                      >  SRR19973080.3987950/1‑150 (MQ=60)

                                                                CTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTG                                                                                      >  SRR19973080.2415278/1‑150 (MQ=60)

                                                                 TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                     >  SRR19973080.840787/1‑150 (MQ=60)

                                                                 TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                     >  SRR19973080.840842/1‑150 (MQ=60)

                                                                     GCAGCTTTTCGTCAATGACATGGGCGCTAAAGCCAATAAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCCGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTG                                                                                 <  SRR19973080.1291580/150‑1 (MQ=60)

                                                                           TTTCGTCAATGATATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTA                                                                           <  SRR19973080.4470005/150‑1 (MQ=60)

                                                                              CGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAG                                                                        >  SRR19973080.260505/1‑150 (MQ=60)

                                                                                   ATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGC                                                                   >  SRR19973080.3723721/1‑150 (MQ=60)

                                                                                   ATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGC                                                                   >  SRR19973080.1910524/1‑150 (MQ=60)

                                                                                   ATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGC                                                                   >  SRR19973080.1905329/1‑150 (MQ=60)

                                                                                    TGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCG                                                                  >  SRR19973080.4469943/1‑150 (MQ=60)

                                                                                    TGACATGGGCGCTAAAGCAAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCG                                                                  <  SRR19973080.3516425/150‑1 (MQ=60)

                                                                                       CATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGG                                                               >  SRR19973080.3490224/1‑150 (MQ=60)

                                                                                        ATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGC                                                              >  SRR19973080.2575096/1‑150 (MQ=60)

                                                                                        ATGGGCGCTAAAGCCAAACAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGC                                                              >  SRR19973080.891172/1‑150 (MQ=60)

                                                                                           GGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATT                                                           <  SRR19973080.3995238/150‑1 (MQ=60)

                                                                                             CGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGC                                                         >  SRR19973080.1184217/1‑150 (MQ=60)

                                                                                              GCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCC                                                        >  SRR19973080.2447484/1‑150 (MQ=60)

                                                                                                TAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAA                                                      >  SRR19973080.1437823/1‑150 (MQ=60)

                                                                                                TAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAA                                                      <  SRR19973080.549028/150‑1 (MQ=60)

                                                                                                  AAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTACTGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                    <  SRR19973080.2705395/150‑1 (MQ=60)

                                                                                                  AAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                    >  SRR19973080.3684988/1‑150 (MQ=60)

                                                                                                  AAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                    >  SRR19973080.1397537/1‑150 (MQ=60)

                                                                                                  AAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                    >  SRR19973080.4126167/1‑150 (MQ=60)

                                                                                                    GCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGC                                                  >  SRR19973080.2290208/1‑150 (MQ=60)

                                                                                                    CCCAAACAAACCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGC                                                  <  SRR19973080.4205095/150‑1 (MQ=60)

                                                                                                       AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                               >  SRR19973080.925345/1‑150 (MQ=60)

                                                                                                       AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                               >  SRR19973080.925454/1‑150 (MQ=60)

                                                                                                       AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                               >  SRR19973080.1606418/1‑150 (MQ=60)

                                                                                                        ATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACA                                              >  SRR19973080.3834926/1‑150 (MQ=60)

                                                                                                            AAACCCGCGACGTATATTGCTGTGCCAGTTGTCGTGAAAGATTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCT                                          <  SRR19973080.200918/150‑1 (MQ=60)

                                                                                                            AAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCT                                          <  SRR19973080.3420723/150‑1 (MQ=60)

                                                                                                            AAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCT                                          >  SRR19973080.3407562/1‑150 (MQ=60)

                                                                                                            AAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCT                                          <  SRR19973080.314314/150‑1 (MQ=60)

                                                                                                             AACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTG                                         >  SRR19973080.2831588/1‑150 (MQ=60)

                                                                                                             AACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTG                                         >  SRR19973080.2831717/1‑150 (MQ=60)

                                                                                                               CCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCG                                       <  SRR19973080.3715754/150‑1 (MQ=60)

                                                                                                               CCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCG                                       >  SRR19973080.2803812/1‑150 (MQ=60)

                                                                                                               CCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCG                                       <  SRR19973080.2567742/150‑1 (MQ=60)

                                                                                                                 AGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCA                                     >  SRR19973080.4280055/1‑150 (MQ=60)

                                                                                                                   CGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACC                                   >  SRR19973080.3735374/1‑150 (MQ=60)

                                                                                                                    GACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCA                                  >  SRR19973080.538488/1‑150 (MQ=60)

                                                                                                                    GACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCA                                  >  SRR19973080.538480/1‑150 (MQ=60)

                                                                                                                    GACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTGCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCA                                  >  SRR19973080.2094710/1‑150 (MQ=60)

                                                                                                                        GATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGG                              >  SRR19973080.1996721/1‑150 (MQ=60)

                                                                                                                        GATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGG                              >  SRR19973080.1996727/1‑150 (MQ=60)

                                                                                                                             TGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTC                         <  SRR19973080.1774603/150‑1 (MQ=60)

                                                                                                                             TGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTC                         <  SRR19973080.1774613/150‑1 (MQ=60)

                                                                                                                             TGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTC                         >  SRR19973080.2722661/1‑150 (MQ=60)

                                                                                                                             TGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTC                         <  SRR19973080.3886521/150‑1 (MQ=60)

                                                                                                                             TGCTGTGCCAGTTGTCGGGCAAGCTTAAGGCCGTCCATAACCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTC                         <  SRR19973080.3886453/150‑1 (MQ=60)

                                                                                                                                 GTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCA                     <  SRR19973080.1195034/150‑1 (MQ=60)

                                                                                                                                 GTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGAAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCA                     <  SRR19973080.1195028/150‑1 (MQ=60)

                                                                                                                                  TGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAT                    <  SRR19973080.4207365/150‑1 (MQ=60)

                                                                                                                                      AGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCA                >  SRR19973080.3729712/1‑150 (MQ=60)

                                                                                                                                        TTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCATATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAT              <  SRR19973080.3473124/150‑1 (MQ=60)

                                                                                                                                        TTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAT              <  SRR19973080.2210305/150‑1 (MQ=60)

                                                                                                                                        TTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAT              <  SRR19973080.2210264/150‑1 (MQ=60)

                                                                                                                                            CGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTA          >  SRR19973080.152378/1‑150 (MQ=60)

                                                                                                                                             GGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAA         >  SRR19973080.3529763/1‑150 (MQ=60)

                                                                                                                                             GGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGAATACAGCAGCTCAATGGTAA         >  SRR19973080.726853/1‑150 (MQ=60)

                                                                                                                                             GGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATATTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACAGGTCTTCAGCAACTCAATGGTAA         >  SRR19973080.726852/1‑150 (MQ=60)

                                                                                                                                               GCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATT       <  SRR19973080.4484569/150‑1 (MQ=60)

                                                                                                                                                    CTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  <  SRR19973080.119592/150‑1 (MQ=60)

                                                                                                                                                    CGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGTATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  <  SRR19973080.646600/150‑1 (MQ=60)

                                                                                                                                                    CGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  <  SRR19973080.3321598/150‑1 (MQ=60)

                                                                                                                                                    CGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  <  SRR19973080.2920477/150‑1 (MQ=60)

ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTG  >  CP009273/1049258‑1049555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`