Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,049,407 G→T 100% T743K (ACG→AAG)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,049,4070GT97.3% 755.5 / 10.0 222T743K (ACG→AAG) torShybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (114/102);  total (116/106)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

TGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCG  >  CP009273/1049260‑1049552
                                                                                                                                                   |                                                                                                                                                 
  aCCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAAt                                                                                                                                               >  2:2165588/1‑150 (MQ=255)
  aCCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAAt                                                                                                                                               <  2:2934584/150‑1 (MQ=255)
        cACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTc                                                                                                                                         >  2:1011742/1‑150 (MQ=255)
         aCTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCg                                                                                                                                        <  1:944756/150‑1 (MQ=255)
          cTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGa                                                                                                                                       >  1:3069728/1‑150 (MQ=255)
           ttCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGat                                                                                                                                      <  2:2675155/150‑1 (MQ=255)
             cACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGatat                                                                                                                                    <  1:3072463/150‑1 (MQ=255)
               cgcgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATcc                                                                                                                                  >  1:3625706/1‑150 (MQ=255)
               cgcgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATcc                                                                                                                                  <  2:2721951/150‑1 (MQ=255)
                 cgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCgg                                                                                                                                >  1:4014932/1‑150 (MQ=255)
                  ggCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGc                                                                                                                               >  2:2116960/1‑150 (MQ=255)
                  ggCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGc                                                                                                                               >  2:3733780/1‑150 (MQ=255)
                  ggCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGc                                                                                                                               >  1:1853894/1‑150 (MQ=255)
                     aCCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGa                                                                                                                            <  1:1045802/150‑1 (MQ=255)
                      ccGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGAt                                                                                                                           <  1:335175/150‑1 (MQ=255)
                        ggTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCa                                                                                                                         <  2:1158762/150‑1 (MQ=255)
                            tAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAt                                                                                                                     >  1:196725/1‑150 (MQ=255)
                            tAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAt                                                                                                                     >  1:3551352/1‑150 (MQ=255)
                              gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATcc                                                                                                                   <  2:559019/150‑1 (MQ=255)
                              gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATcc                                                                                                                   <  2:558920/150‑1 (MQ=255)
                               ggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATcca                                                                                                                  <  2:1330123/150‑1 (MQ=255)
                                 aTAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATccacc                                                                                                                <  1:488319/150‑1 (MQ=255)
                                 aTAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATccacc                                                                                                                <  1:3944409/150‑1 (MQ=255)
                                       ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                          <  2:420859/150‑1 (MQ=255)
                                       ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                          <  1:2333156/150‑1 (MQ=255)
                                       ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                          <  2:3769251/150‑1 (MQ=255)
                                       ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                          <  2:866860/150‑1 (MQ=255)
                                        cgcgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCgg                                                                                                         >  2:2262704/1‑150 (MQ=255)
                                        cgcgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCgg                                                                                                         >  2:2262711/1‑150 (MQ=255)
                                            gAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgcagc                                                                                                     >  1:1233821/1‑150 (MQ=255)
                                             aataatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgcagca                                                                                                    >  1:1640603/1‑150 (MQ=255)
                                               taatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                  >  2:3947956/1‑150 (MQ=255)
                                                aatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAAc                                                                                                 >  2:1148172/1‑150 (MQ=255)
                                                  tGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACgg                                                                                               >  2:1224472/1‑150 (MQ=255)
                                                  tGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACgg                                                                                               >  2:432899/1‑150 (MQ=255)
                                                    aaCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGtt                                                                                             <  2:1378444/150‑1 (MQ=255)
                                                     aCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTc                                                                                            >  1:3393964/1‑150 (MQ=255)
                                                     aCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTc                                                                                            >  2:1769259/1‑150 (MQ=255)
                                                     aCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTc                                                                                            <  2:3185352/150‑1 (MQ=255)
                                                      cTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCg                                                                                           <  1:1001647/150‑1 (MQ=255)
                                                        ggTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCt                                                                                         <  2:3461069/150‑1 (MQ=255)
                                                        ggTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCt                                                                                         <  2:3461163/150‑1 (MQ=255)
                                                        ggTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCt                                                                                         >  1:3846173/1‑150 (MQ=255)
                                                            cGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTAttt                                                                                     >  1:1365827/1‑150 (MQ=255)
                                                             gCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTAtttt                                                                                    <  1:589663/150‑1 (MQ=255)
                                                             gCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTAtttt                                                                                    <  1:3250555/150‑1 (MQ=255)
                                                             gCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTAtttt                                                                                    <  1:1689795/150‑1 (MQ=255)
                                                              cTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTg                                                                                   <  2:966469/150‑1 (MQ=255)
                                                                ggCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCa                                                                                 <  2:3312618/150‑1 (MQ=255)
                                                                 gcgcAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAg                                                                                <  2:1564954/150‑1 (MQ=255)
                                                                     aGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGtc                                                                            >  1:2910108/1‑150 (MQ=255)
                                                                      gCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGtct                                                                           <  2:3037809/150‑1 (MQ=255)
                                                                         tttCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTa                                                                        <  1:3324945/150‑1 (MQ=255)
                                                                           tCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTaaa                                                                      <  2:2664256/150‑1 (MQ=255)
                                                                           tCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTaaa                                                                      <  2:2457475/150‑1 (MQ=255)
                                                                            cGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAg                                                                     <  2:1300401/150‑1 (MQ=255)
                                                                             gTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGc                                                                    >  2:3845057/1‑150 (MQ=255)
                                                                             gTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGc                                                                    >  2:3258015/1‑150 (MQ=255)
                                                                               cAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCt                                                                  <  1:3429414/150‑1 (MQ=255)
                                                                                aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                 >  1:3868789/1‑150 (MQ=255)
                                                                                aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                 <  2:4013696/150‑1 (MQ=255)
                                                                                  tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                               >  2:2068063/1‑150 (MQ=255)
                                                                                  tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                               <  1:530162/150‑1 (MQ=255)
                                                                                  tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                               <  1:560810/150‑1 (MQ=255)
                                                                                        gggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAt                                                         <  2:508444/150‑1 (MQ=255)
                                                                                            gcTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGcc                                                     <  2:1535123/150‑1 (MQ=255)
                                                                                              tAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCaa                                                   >  1:1225925/1‑150 (MQ=255)
                                                                                               aaaGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAc                                                  >  2:1976643/1‑150 (MQ=255)
                                                                                                  gCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGc                                               <  2:1066454/150‑1 (MQ=255)
                                                                                                  gCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGc                                               >  1:3568413/1‑150 (MQ=255)
                                                                                                    cAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaa                                             >  2:54250/1‑150 (MQ=255)
                                                                                                     aaTCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaac                                            <  1:937567/150‑1 (MQ=255)
                                                                                                       tCAAAAACAGCGACGGATATTGCTGTGCCATTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATATTTCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaacaa                                          >  2:1247467/1‑150 (MQ=255)
                                                                                                        cAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAAt                                         >  1:2748774/1‑150 (MQ=255)
                                                                                                        cAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAAt                                         >  1:663186/1‑150 (MQ=255)
                                                                                                         aaaaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATc                                        >  1:2433674/1‑150 (MQ=255)
                                                                                                         aaaaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATc                                        >  1:2433653/1‑150 (MQ=255)
                                                                                                         aaaaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATc                                        >  2:311090/1‑150 (MQ=255)
                                                                                                           aaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTg                                      >  1:1498780/1‑150 (MQ=255)
                                                                                                           aaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTg                                      >  1:3722500/1‑150 (MQ=255)
                                                                                                            aCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTgc                                     <  1:175527/150‑1 (MQ=255)
                                                                                                              cAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTgcgc                                   >  2:4137207/1‑150 (MQ=255)
                                                                                                                gCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcac                                 <  1:3869504/150‑1 (MQ=255)
                                                                                                                  gACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacca                               >  2:93492/1‑150 (MQ=255)
                                                                                                                     ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCTGCAGATAAAAATCCAACAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGACAACAGCAACAATCTGCGCACAACTg                            >  1:768202/1‑150 (MQ=255)
                                                                                                                     ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                            <  2:1022444/150‑1 (MQ=255)
                                                                                                                     ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                            >  1:768280/1‑150 (MQ=255)
                                                                                                                       atatTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGt                          >  2:929665/1‑150 (MQ=255)
                                                                                                                         atTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGttt                        >  2:1904539/1‑150 (MQ=255)
                                                                                                                           tGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTc                      <  2:3051598/150‑1 (MQ=255)
                                                                                                                            gCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCa                     >  1:2546303/1‑150 (MQ=255)
                                                                                                                               gtgCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCa                  <  2:688156/150‑1 (MQ=255)
                                                                                                                               gtgCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAGTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGct                  <  2:898160/150‑2 (MQ=255)
                                                                                                                                  ccAGTTGTCGGTCTAGCTTAATGCCGTCTATATCCTGCAGATCAAAATCCACCAGTGCGGCTGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCTTTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAtct               <  1:719490/150‑1 (MQ=255)
                                                                                                                                  ccAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAtct               <  1:3017952/150‑1 (MQ=255)
                                                                                                                                     gTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCaa            >  1:1231051/1‑150 (MQ=255)
                                                                                                                                       tGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg          <  1:148416/150‑1 (MQ=255)
                                                                                                                                       tGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg          <  2:3396782/150‑1 (MQ=255)
                                                                                                                                            ggCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAAt     <  2:2495430/150‑1 (MQ=255)
                                                                                                                                              cAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTc   <  1:1587120/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCG  >  CP009273/1049260‑1049552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGG  >  CP009273/1049264‑1049556
                                                                                                                                               |                                                                                                                                                     
CGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGC                                                                                                                                                 >  SRR19973074.928564/1‑150 (MQ=60)
  AGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCG                                                                                                                                               <  SRR19973074.3518829/150‑1 (MQ=60)
   GCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGT                                                                                                                                              >  SRR19973074.3986437/1‑150 (MQ=60)
   GCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGT                                                                                                                                              >  SRR19973074.1304524/1‑150 (MQ=60)
     ACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCG                                                                                                                                            <  SRR19973074.946552/150‑1 (MQ=60)
      CTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGA                                                                                                                                           >  SRR19973074.3075380/1‑150 (MQ=60)
         CACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATAT                                                                                                                                        <  SRR19973074.3078119/150‑1 (MQ=60)
           CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCC                                                                                                                                      <  SRR19973074.752000/150‑1 (MQ=60)
           CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCC                                                                                                                                      >  SRR19973074.3632406/1‑150 (MQ=60)
             CGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGG                                                                                                                                    >  SRR19973074.4022356/1‑150 (MQ=60)
              GGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGC                                                                                                                                   >  SRR19973074.1857433/1‑150 (MQ=60)
                 ACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGA                                                                                                                                <  SRR19973074.1047659/150‑1 (MQ=60)
                 ACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGA                                                                                                                                <  SRR19973074.1047724/150‑1 (MQ=60)
                  CCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGAT                                                                                                                               <  SRR19973074.335785/150‑1 (MQ=60)
                        TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                         >  SRR19973074.197143/1‑150 (MQ=60)
                        TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                         >  SRR19973074.3557900/1‑150 (MQ=60)
                             ATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACC                                                                                                                    <  SRR19973074.489299/150‑1 (MQ=60)
                             ATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACC                                                                                                                    <  SRR19973074.3951677/150‑1 (MQ=60)
                             ATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACC                                                                                                                    >  SRR19973074.3817141/1‑150 (MQ=60)
                                   CCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCG                                                                                                              <  SRR19973074.2337505/150‑1 (MQ=60)
                                      CGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAAACAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCA                                                                                                           >  SRR19973074.148995/1‑150 (MQ=60)
                                        GAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGC                                                                                                         >  SRR19973074.1236185/1‑150 (MQ=60)
                                         AATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCA                                                                                                        >  SRR19973074.1643718/1‑150 (MQ=60)
                                         AATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCA                                                                                                        >  SRR19973074.1643708/1‑150 (MQ=60)
                                            AATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAAC                                                                                                     <  SRR19973074.2505903/150‑1 (MQ=60)
                                             ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                    >  SRR19973074.1673027/1‑150 (MQ=60)
                                             ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                    <  SRR19973074.3076573/150‑1 (MQ=60)
                                               GAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGT                                                                                                  >  SRR19973074.1339358/1‑150 (MQ=60)
                                                 ACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTC                                                                                                >  SRR19973074.3400351/1‑150 (MQ=60)
                                                 ACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTC                                                                                                >  SRR19973074.3400248/1‑150 (MQ=60)
                                                 ACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTC                                                                                                >  SRR19973074.3400230/1‑150 (MQ=60)
                                                  CTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCG                                                                                               <  SRR19973074.2816587/150‑1 (MQ=60)
                                                  CTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCG                                                                                               <  SRR19973074.1003507/150‑1 (MQ=60)
                                                    GGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCT                                                                                             >  SRR19973074.3853188/1‑150 (MQ=60)
                                                     GTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGACGATATACGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTA                                                                                            >  SRR19973074.303736/1‑150 (MQ=60)
                                                        CGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTT                                                                                         >  SRR19973074.1368354/1‑150 (MQ=60)
                                                         GCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTT                                                                                        <  SRR19973074.3256588/150‑1 (MQ=60)
                                                         GCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTT                                                                                        >  SRR19973074.1588161/1‑150 (MQ=60)
                                                         GCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTT                                                                                        <  SRR19973074.1692953/150‑1 (MQ=60)
                                                         GCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTT                                                                                        <  SRR19973074.3256572/150‑1 (MQ=60)
                                                         GCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTT                                                                                        <  SRR19973074.590799/150‑1 (MQ=60)
                                                          CTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTG                                                                                       <  SRR19973074.3620006/150‑1 (MQ=60)
                                                           TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                      <  SRR19973074.2795662/150‑1 (MQ=60)
                                                           TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                      >  SRR19973074.1822117/1‑150 (MQ=60)
                                                           TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                      >  SRR19973074.187724/1‑150 (MQ=60)
                                                            GGCGCAGCGTTTCGTCAATGACATGGGCGCAAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCA                                                                                     <  SRR19973074.123073/150‑1 (MQ=60)
                                                               GCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTG                                                                                  <  SRR19973074.3136874/150‑1 (MQ=60)
                                                                 AGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTC                                                                                <  SRR19973074.3708272/150‑1 (MQ=60)
                                                                 AGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTC                                                                                >  SRR19973074.4182579/1‑150 (MQ=60)
                                                                 AGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTC                                                                                >  SRR19973074.2915514/1‑150 (MQ=60)
                                                                    GTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCT                                                                             >  SRR19973074.3898537/1‑150 (MQ=60)
                                                                     TTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTA                                                                            <  SRR19973074.3331121/150‑1 (MQ=60)
                                                                     TTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTA                                                                            >  SRR19973074.3225390/1‑150 (MQ=60)
                                                                         GTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGC                                                                        >  SRR19973074.1163695/1‑150 (MQ=60)
                                                                         GTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGC                                                                        >  SRR19973074.523379/1‑150 (MQ=60)
                                                                           CAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCT                                                                      <  SRR19973074.3435738/150‑1 (MQ=60)
                                                                           CAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCT                                                                      <  SRR19973074.3435775/150‑1 (MQ=60)
                                                                            AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                     >  SRR19973074.3875863/1‑150 (MQ=60)
                                                                            AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                     >  SRR19973074.3875859/1‑150 (MQ=60)
                                                                            AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                     >  SRR19973074.413197/1‑150 (MQ=60)
                                                                              TGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCG                                                                   <  SRR19973074.531221/150‑1 (MQ=60)
                                                                              TGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCG                                                                   <  SRR19973074.561913/150‑1 (MQ=60)
                                                                                          TAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAA                                                       >  SRR19973074.1228273/1‑150 (MQ=60)
                                                                                           AAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAC                                                      >  SRR19973074.2736482/1‑150 (MQ=60)
                                                                                              GCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGC                                                   >  SRR19973074.3574995/1‑150 (MQ=60)
                                                                                                CAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAA                                                 >  SRR19973074.3742651/1‑150 (MQ=60)
                                                                                                 AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                                <  SRR19973074.939487/150‑1 (MQ=60)
                                                                                                 AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                                <  SRR19973074.939478/150‑1 (MQ=60)
                                                                                                 AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                                <  SRR19973074.939356/150‑1 (MQ=60)
                                                                                                  ATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACA                                               <  SRR19973074.718597/150‑1 (MQ=60)
                                                                                                    CAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAAT                                             >  SRR19973074.2753837/1‑150 (MQ=60)
                                                                                                    CAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAAT                                             >  SRR19973074.664413/1‑150 (MQ=60)
                                                                                                     AAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATC                                            >  SRR19973074.2438156/1‑150 (MQ=60)
                                                                                                     AAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATC                                            >  SRR19973074.2438136/1‑150 (MQ=60)
                                                                                                     AAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATC                                            >  SRR19973074.2438174/1‑150 (MQ=60)
                                                                                                     AAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATC                                            >  SRR19973074.2438177/1‑150 (MQ=60)
                                                                                                     AAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATC                                            <  SRR19973074.3566825/150‑1 (MQ=60)
                                                                                                      AAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCT                                           >  SRR19973074.162240/1‑150 (MQ=60)
                                                                                                       AACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTG                                          >  SRR19973074.3729364/1‑150 (MQ=60)
                                                                                                       AACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTG                                          >  SRR19973074.144283/1‑150 (MQ=60)
                                                                                                       AACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTG                                          >  SRR19973074.1501611/1‑150 (MQ=60)
                                                                                                        ACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGC                                         <  SRR19973074.175908/150‑1 (MQ=60)
                                                                                                        ACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGC                                         >  SRR19973074.2969632/1‑150 (MQ=60)
                                                                                                          CAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGC                                       >  SRR19973074.589478/1‑150 (MQ=60)
                                                                                                            GCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCAC                                     <  SRR19973074.3876573/150‑1 (MQ=60)
                                                                                                            GCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCAC                                     <  SRR19973074.3876582/150‑1 (MQ=60)
                                                                                                                 GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCTGCAGATAAAAATCCAACAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGACAACAGCAACAATCTGCGCACAACTG                                >  SRR19973074.769676/1‑150 (MQ=60)
                                                                                                                 GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                >  SRR19973074.769754/1‑150 (MQ=60)
                                                                                                                 GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                >  SRR19973074.769726/1‑150 (MQ=60)
                                                                                                                  GATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGG                               <  SRR19973074.1235379/150‑1 (MQ=60)
                                                                                                                   ATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGT                              >  SRR19973074.3346021/1‑150 (MQ=60)
                                                                                                                     ATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTT                            <  SRR19973074.1701818/150‑1 (MQ=60)
                                                                                                                       TGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTC                          <  SRR19973074.2102099/150‑1 (MQ=60)
                                                                                                                        GCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCA                         >  SRR19973074.2551054/1‑150 (MQ=60)
                                                                                                                              CCAGTTGTCGGTCTAGCTTAATGCCGTCTATATCCTGCAGATCAAAATCCACCAGTGCGGCTGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCTTTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCT                   <  SRR19973074.720838/150‑1 (MQ=60)
                                                                                                                              CCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCT                   <  SRR19973074.3023540/150‑1 (MQ=60)
                                                                                                                                 GTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAA                >  SRR19973074.1233409/1‑150 (MQ=60)
                                                                                                                                   TGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATG              <  SRR19973074.148753/150‑1 (MQ=60)
                                                                                                                                     TCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGT            <  SRR19973074.548700/150‑1 (MQ=60)
                                                                                                                                      CGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTA           <  SRR19973074.2001605/150‑1 (MQ=60)
                                                                                                                                      CGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTA           >  SRR19973074.603013/1‑150 (MQ=60)
                                                                                                                                      CGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTA           >  SRR19973074.602976/1‑150 (MQ=60)
                                                                                                                                          CAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC       <  SRR19973074.1590134/150‑1 (MQ=60)
                                                                                                                                               TTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGG  <  SRR19973074.2112392/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                     
CGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGG  >  CP009273/1049264‑1049556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑