Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,049,407 G→T 92.7% T743K (ACG→AAG)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,049,4070GT92.7% 698.5 / 37.3 220T743K (ACG→AAG) torShybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base G (5/11);  new base T (93/111);  total (98/122)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGC  >  CP009273/1049260‑1049553
                                                                                                                                                   |                                                                                                                                                  
tGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTa                                                                                                                                                  <  2:2419830/150‑1 (MQ=255)
  aCCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAAt                                                                                                                                                <  2:2597777/150‑1 (MQ=255)
  aCCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAAt                                                                                                                                                <  2:3475062/150‑1 (MQ=255)
   ccGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATg                                                                                                                                               <  1:3055147/150‑1 (MQ=255)
   ccGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATg                                                                                                                                               >  1:1719757/1‑150 (MQ=255)
    cGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTTCGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGc                                                                                                                                              <  1:1939269/150‑1 (MQ=255)
    cGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGc                                                                                                                                              >  2:3837556/1‑150 (MQ=255)
     gAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGcc                                                                                                                                             <  1:3179223/150‑1 (MQ=255)
     gAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGcc                                                                                                                                             <  1:990429/150‑1 (MQ=255)
       gCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGt                                                                                                                                           <  1:2403005/150‑1 (MQ=255)
           ttCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGat                                                                                                                                       <  2:4006863/150‑1 (MQ=255)
            tCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGata                                                                                                                                      >  1:3010247/1‑150 (MQ=255)
              aCGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATc                                                                                                                                    <  2:945822/150‑1 (MQ=255)
              aCGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATc                                                                                                                                    <  2:945728/150‑1 (MQ=255)
              aCGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATc                                                                                                                                    <  2:858220/150‑1 (MQ=255)
               cgcgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATcc                                                                                                                                   <  2:3964206/150‑1 (MQ=255)
                gcgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCg                                                                                                                                  <  2:2268633/150‑1 (MQ=255)
                 cgGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCgg                                                                                                                                 >  1:2497665/1‑150 (MQ=255)
                   gCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCa                                                                                                                               >  2:1850028/1‑150 (MQ=255)
                   gCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCa                                                                                                                               >  1:2338464/1‑150 (MQ=255)
                    cACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAg                                                                                                                              >  1:944340/1‑150 (MQ=255)
                    cACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAg                                                                                                                              <  2:4111253/150‑1 (MQ=255)
                      ccGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGAt                                                                                                                            >  1:2022876/1‑150 (MQ=255)
                        ggTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCa                                                                                                                          <  2:2218363/150‑1 (MQ=255)
                         gTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaa                                                                                                                         >  1:196609/1‑150 (MQ=255)
                          tttAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaaa                                                                                                                        >  1:3951871/1‑150 (MQ=255)
                             aGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAACACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATc                                                                                                                     <  1:1753830/150‑1 (MQ=255)
                             aGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATc                                                                                                                     >  1:1501944/1‑150 (MQ=255)
                              gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATcc                                                                                                                    <  2:795867/150‑1 (MQ=255)
                               ggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATcca                                                                                                                   >  2:3452943/1‑150 (MQ=255)
                                   aaTCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAg                                                                                                               <  2:1563088/150‑1 (MQ=255)
                                    aTCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGt                                                                                                              <  1:1626941/150‑1 (MQ=255)
                                       ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                           >  2:621515/1‑150 (MQ=255)
                                       ccGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCg                                                                                                           >  2:2792664/1‑150 (MQ=255)
                                          cgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgca                                                                                                        >  1:1915304/1‑150 (MQ=255)
                                          cgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgca                                                                                                        >  2:4265704/1‑150 (MQ=255)
                                          cgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgca                                                                                                        >  2:3293892/1‑150 (MQ=255)
                                            gAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgcagc                                                                                                      <  2:1538367/150‑1 (MQ=255)
                                                   gAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGt                                                                                               <  1:3115641/150‑1 (MQ=255)
                                                   gAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGt                                                                                               <  1:2725374/150‑1 (MQ=255)
                                                    aaCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGtt                                                                                              <  2:3934864/150‑1 (MQ=255)
                                                    aaCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGtt                                                                                              >  1:2116082/1‑150 (MQ=255)
                                                      cTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCg                                                                                            <  2:678641/150‑1 (MQ=255)
                                                         gTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTa                                                                                         >  1:1777891/1‑150 (MQ=255)
                                                            cGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAACCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCCGTGCGGCAGCAAACGGTTCGCTAttt                                                                                      <  1:2023287/150‑1 (MQ=255)
                                                               tGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGc                                                                                   >  2:1979798/1‑150 (MQ=255)
                                                                     aGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGtc                                                                             <  2:3437923/150‑1 (MQ=255)
                                                                         tttCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTa                                                                         <  2:3062670/150‑1 (MQ=255)
                                                                             gTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGc                                                                     >  1:1007186/1‑150 (MQ=255)
                                                                              tCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGcc                                                                    >  2:3739896/1‑150 (MQ=255)
                                                                                aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                  >  1:969265/1‑150 (MQ=255)
                                                                                aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                  <  1:4207896/150‑1 (MQ=255)
                                                                                aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                  >  1:643059/1‑150 (MQ=255)
                                                                                  tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                                >  2:334836/1‑150 (MQ=255)
                                                                                  tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                                >  2:2719335/1‑150 (MQ=255)
                                                                                        gggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAt                                                          <  1:3368673/150‑1 (MQ=255)
                                                                                         ggCGCTAACGCCAATCAAAACCACCCACGGATATTGCTGTGCCCGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCACAATCCACCAGTGCGGCCGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGTCAtt                                                         <  2:1633728/150‑1 (MQ=255)
                                                                                         ggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAtt                                                         >  1:2830166/1‑150 (MQ=255)
                                                                                         ggCGCTAAAGCCAATAAAAACCCGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAtt                                                         <  1:1830185/150‑1 (MQ=255)
                                                                                           cgcTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGc                                                       >  1:3512955/1‑150 (MQ=255)
                                                                                           cgcTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGc                                                       >  1:1744036/1‑150 (MQ=255)
                                                                                              tAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCaa                                                    >  2:3300127/1‑150 (MQ=255)
                                                                                               aaaGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAc                                                   >  1:2555603/1‑150 (MQ=255)
                                                                                               aaaGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAc                                                   >  1:22204/1‑150 (MQ=255)
                                                                                                     aaTCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaac                                             >  1:736013/1‑150 (MQ=255)
                                                                                                     aaTCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaac                                             <  1:4244038/150‑1 (MQ=255)
                                                                                                        cAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAAt                                          <  2:2904746/150‑1 (MQ=255)
                                                                                                        cAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAAt                                          >  2:603987/1‑150 (MQ=255)
                                                                                                              cAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTgcgc                                    >  2:636086/1‑150 (MQ=255)
                                                                                                              cAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTgcgc                                    >  1:1157084/1‑150 (MQ=255)
                                                                                                                  gACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacca                                <  1:2698146/150‑1 (MQ=255)
                                                                                                                    cGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACt                              <  1:1678941/150‑1 (MQ=255)
                                                                                                                    cGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACt                              <  1:3835552/150‑1 (MQ=255)
                                                                                                                    cGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACt                              <  1:455383/150‑1 (MQ=255)
                                                                                                                     ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                             >  1:1027668/1‑150 (MQ=255)
                                                                                                                     ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                             >  1:1815839/1‑150 (MQ=255)
                                                                                                                     ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTg                             <  1:479997/150‑1 (MQ=255)
                                                                                                                       atatTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGt                           <  2:2591135/150‑1 (MQ=255)
                                                                                                                       atatTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGt                           >  1:3169335/1‑150 (MQ=255)
                                                                                                                       atatTGCTGTGCCAGGTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGt                           <  2:3911059/150‑1 (MQ=255)
                                                                                                                        tatTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGtt                          >  2:1944590/1‑150 (MQ=255)
                                                                                                                         atTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGttt                         <  1:1881247/150‑1 (MQ=255)
                                                                                                                               gtgCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCa                   >  2:1144857/1‑150 (MQ=255)
                                                                                                                                tgCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAt                  >  2:1109335/1‑150 (MQ=255)
                                                                                                                                  ccAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAtct                <  2:527307/150‑1 (MQ=255)
                                                                                                                                    aGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCa              <  1:1185759/150‑1 (MQ=255)
                                                                                                                                    aGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCa              >  2:1679429/1‑150 (MQ=255)
                                                                                                                                      ttGTCGGGCAAGCTTACTGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAt            <  1:4102590/150‑1 (MQ=255)
                                                                                                                                       tGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg           <  2:1649764/150‑1 (MQ=255)
                                                                                                                                       tGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg           <  1:4325959/150‑1 (MQ=255)
                                                                                                                                       tGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg           <  2:1703525/150‑1 (MQ=255)
                                                                                                                                       tGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCCATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg           <  2:3854911/150‑1 (MQ=255)
                                                                                                                                         tCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGt         <  2:2688591/150‑1 (MQ=255)
                                                                                                                                          cGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTa        <  2:1418471/150‑1 (MQ=255)
                                                                                                                                            ggCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAAt      >  2:1452923/1‑150 (MQ=255)
                                                                                                                                            ggCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAAt      >  2:3124158/1‑150 (MQ=255)
                                                                                                                                              cAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTc    >  2:238028/1‑150 (MQ=255)
                                                                                                                                               aaGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCg   >  2:2519968/1‑150 (MQ=255)
                                                                                                                                               aaGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCg   >  2:2771657/1‑150 (MQ=255)
                                                                                                                                                aGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAAGTCGc  >  2:3164829/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGC  >  CP009273/1049260‑1049553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGG  >  CP009273/1049258‑1049556
                                                                                                                                                     |                                                                                                                                                     
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCT                                                                                                                                                       <  SRR19973079.3628152/150‑1 (MQ=60)
   GACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAA                                                                                                                                                    >  SRR19973079.4250017/1‑150 (MQ=60)
   GACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAA                                                                                                                                                    >  SRR19973079.464325/1‑150 (MQ=60)
     CCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATG                                                                                                                                                  <  SRR19973079.3071178/150‑1 (MQ=60)
     CCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATG                                                                                                                                                  <  SRR19973079.3070964/150‑1 (MQ=60)
     CCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATG                                                                                                                                                  >  SRR19973079.1728926/1‑150 (MQ=60)
     CCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATG                                                                                                                                                  >  SRR19973079.1728854/1‑150 (MQ=60)
      CGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTTCGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGC                                                                                                                                                 <  SRR19973079.1949607/150‑1 (MQ=60)
       GAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCC                                                                                                                                                <  SRR19973079.996030/150‑1 (MQ=60)
       GAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCC                                                                                                                                                <  SRR19973079.3195488/150‑1 (MQ=60)
         GCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGT                                                                                                                                              <  SRR19973079.2415406/150‑1 (MQ=60)
            CTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGA                                                                                                                                           <  SRR19973079.2347929/150‑1 (MQ=60)
              TCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATA                                                                                                                                         >  SRR19973079.3025797/1‑150 (MQ=60)
                   CGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGG                                                                                                                                    <  SRR19973079.184727/150‑1 (MQ=60)
                   CGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGG                                                                                                                                    <  SRR19973079.2201351/150‑1 (MQ=60)
                   CGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGG                                                                                                                                    >  SRR19973079.2510363/1‑150 (MQ=60)
                     GCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCA                                                                                                                                  >  SRR19973079.2350609/1‑150 (MQ=60)
                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAG                                                                                                                                 >  SRR19973079.949766/1‑150 (MQ=60)
                      CACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAG                                                                                                                                 >  SRR19973079.949961/1‑150 (MQ=60)
                        CCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGAT                                                                                                                               >  SRR19973079.2033531/1‑150 (MQ=60)
                           GTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAA                                                                                                                            >  SRR19973079.198018/1‑150 (MQ=60)
                            TTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAA                                                                                                                           >  SRR19973079.3971787/1‑150 (MQ=60)
                            TTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAA                                                                                                                           >  SRR19973079.1773683/1‑150 (MQ=60)
                               AGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAACACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATC                                                                                                                        <  SRR19973079.1763085/150‑1 (MQ=60)
                               AGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATC                                                                                                                        <  SRR19973079.1763554/150‑1 (MQ=60)
                               AGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATC                                                                                                                        >  SRR19973079.1510216/1‑150 (MQ=60)
                               AGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATC                                                                                                                        >  SRR19973079.1512192/1‑150 (MQ=60)
                                      ATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGT                                                                                                                 <  SRR19973079.1635190/150‑1 (MQ=60)
                                      ATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGT                                                                                                                 <  SRR19973079.1635834/150‑1 (MQ=60)
                                      ATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGT                                                                                                                 <  SRR19973079.2651374/150‑1 (MQ=60)
                                      ATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGT                                                                                                                 <  SRR19973079.2651610/150‑1 (MQ=60)
                                       TCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCATTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTG                                                                                                                <  SRR19973079.398976/150‑1 (MQ=60)
                                            CGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCA                                                                                                           >  SRR19973079.1925572/1‑150 (MQ=60)
                                            CGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCA                                                                                                           >  SRR19973079.1925317/1‑150 (MQ=60)
                                            CGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCA                                                                                                           >  SRR19973079.1925527/1‑150 (MQ=60)
                                            CGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCA                                                                                                           >  SRR19973079.1150185/1‑150 (MQ=60)
                                                    TGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGG                                                                                                   <  SRR19973079.3481808/150‑1 (MQ=60)
                                                    TGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGG                                                                                                   >  SRR19973079.2974554/1‑150 (MQ=60)
                                                     GAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGT                                                                                                  <  SRR19973079.2739103/150‑1 (MQ=60)
                                                     GAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGT                                                                                                  <  SRR19973079.2739467/150‑1 (MQ=60)
                                                     GAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGT                                                                                                  <  SRR19973079.3131705/150‑1 (MQ=60)
                                                      AACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTT                                                                                                 >  SRR19973079.2127043/1‑150 (MQ=60)
                                                      AACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTT                                                                                                 >  SRR19973079.991579/1‑150 (MQ=60)
                                                       ACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTC                                                                                                <  SRR19973079.2032758/150‑1 (MQ=60)
                                                           GTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTA                                                                                            >  SRR19973079.1787201/1‑150 (MQ=60)
                                                           GTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTA                                                                                            <  SRR19973079.4215617/150‑1 (MQ=60)
                                                           GTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTA                                                                                            >  SRR19973079.1953428/1‑150 (MQ=60)
                                                              CGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAACCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCCGTGCGGCAGCAAACGGTTCGCTATTT                                                                                         <  SRR19973079.2033943/150‑1 (MQ=60)
                                                              CGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAAACAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTT                                                                                         <  SRR19973079.2033827/150‑1 (MQ=60)
                                                                 TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                      >  SRR19973079.2209375/1‑150 (MQ=60)
                                                                      CAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGT                                                                                 <  SRR19973079.1749380/150‑1 (MQ=60)
                                                                      CAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGT                                                                                 <  SRR19973079.4338949/150‑1 (MQ=60)
                                                                      CAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGT                                                                                 >  SRR19973079.4368253/1‑150 (MQ=60)
                                                                               GTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGC                                                                        >  SRR19973079.1012859/1‑150 (MQ=60)
                                                                                  AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                     >  SRR19973079.974787/1‑150 (MQ=60)
                                                                                  AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                     <  SRR19973079.4229203/150‑1 (MQ=60)
                                                                                  AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                     >  SRR19973079.646707/1‑150 (MQ=60)
                                                                                   ATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGC                                                                    >  SRR19973079.665414/1‑150 (MQ=60)
                                                                                      ACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCG                                                                 >  SRR19973079.3224908/1‑150 (MQ=60)
                                                                                          GGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAT                                                             <  SRR19973079.3386016/150‑1 (MQ=60)
                                                                                           GGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATT                                                            >  SRR19973079.2844622/1‑150 (MQ=60)
                                                                                           GGCGCTAAAGCCAATAAAAACCCGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATT                                                            <  SRR19973079.1839792/150‑1 (MQ=60)
                                                                                             CGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGC                                                          >  SRR19973079.1753265/1‑150 (MQ=60)
                                                                                             CGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGC                                                          >  SRR19973079.3531022/1‑150 (MQ=60)
                                                                                              GCTAAAGCCAATCAAAACCCGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCC                                                         <  SRR19973079.1261640/150‑1 (MQ=60)
                                                                                               CTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCA                                                        <  SRR19973079.312027/150‑1 (MQ=60)
                                                                                               CTAAAGCAAATCAAACCCAGCGACGGATATTGCTGTGCCTGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCA                                                        <  SRR19973079.806684/150‑1 (MQ=60)
                                                                                                 AAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAC                                                      >  SRR19973079.2568571/1‑150 (MQ=60)
                                                                                                 AAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAC                                                      >  SRR19973079.22465/1‑150 (MQ=60)
                                                                                                  AAGCCAAACAAAACCAGCGACGTATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                     <  SRR19973079.827022/150‑1 (MQ=60)
                                                                                                       AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                                >  SRR19973079.739916/1‑150 (MQ=60)
                                                                                                       AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                                <  SRR19973079.4265515/150‑1 (MQ=60)
                                                                                                       AATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAAC                                                <  SRR19973079.4266471/150‑1 (MQ=60)
                                                                                                                CAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGC                                       >  SRR19973079.1163733/1‑150 (MQ=60)
                                                                                                                    GACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCA                                   <  SRR19973079.2712113/150‑1 (MQ=60)
                                                                                                                      CGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACT                                 <  SRR19973079.2479974/150‑1 (MQ=60)
                                                                                                                      CGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACT                                 <  SRR19973079.458124/150‑1 (MQ=60)
                                                                                                                      CGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACT                                 <  SRR19973079.3855142/150‑1 (MQ=60)
                                                                                                                      CGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACT                                 <  SRR19973079.1687988/150‑1 (MQ=60)
                                                                                                                       GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                <  SRR19973079.482905/150‑1 (MQ=60)
                                                                                                                       GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                >  SRR19973079.1825267/1‑150 (MQ=60)
                                                                                                                       GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                >  SRR19973079.1825302/1‑150 (MQ=60)
                                                                                                                       GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                <  SRR19973079.1661730/150‑1 (MQ=60)
                                                                                                                       GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                                >  SRR19973079.1033390/1‑150 (MQ=60)
                                                                                                                         ATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGT                              >  SRR19973079.3185715/1‑150 (MQ=60)
                                                                                                                         ATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGT                              >  SRR19973079.3185568/1‑150 (MQ=60)
                                                                                                                           ATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTT                            <  SRR19973079.1891252/150‑1 (MQ=60)
                                                                                                                            TTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTT                           <  SRR19973079.3143152/150‑1 (MQ=60)
                                                                                                                                   GCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATC                    >  SRR19973079.3069633/1‑150 (MQ=60)
                                                                                                                                    CCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCT                   >  SRR19973079.1926244/1‑150 (MQ=60)
                                                                                                                                    CCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCT                   <  SRR19973079.1756084/150‑1 (MQ=60)
                                                                                                                                      AGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCA                 <  SRR19973079.1192585/150‑1 (MQ=60)
                                                                                                                                        TTGTCGGGCAAGCTTACTGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAT               <  SRR19973079.4123388/150‑1 (MQ=60)
                                                                                                                                         TGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATG              <  SRR19973079.3810834/150‑1 (MQ=60)
                                                                                                                                         TGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATG              <  SRR19973079.4347707/150‑1 (MQ=60)
                                                                                                                                         TGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATG              >  SRR19973079.1180403/1‑150 (MQ=60)
                                                                                                                                          GTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGG             <  SRR19973079.2383683/150‑1 (MQ=60)
                                                                                                                                           TCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGT            <  SRR19973079.625099/150‑1 (MQ=60)
                                                                                                                                           TCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGT            <  SRR19973079.2806985/150‑1 (MQ=60)
                                                                                                                                            CGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTA           <  SRR19973079.1015351/150‑1 (MQ=60)
                                                                                                                                               GCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATT        >  SRR19973079.3913745/1‑150 (MQ=60)
                                                                                                                                                CAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC       <  SRR19973079.3567347/150‑1 (MQ=60)
                                                                                                                                                CAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC       <  SRR19973079.2550075/150‑1 (MQ=60)
                                                                                                                                                CAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGGGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC       <  SRR19973079.1834571/150‑1 (MQ=60)
                                                                                                                                                     TTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGG  <  SRR19973079.2382612/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGCTGG  >  CP009273/1049258‑1049556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑