Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,049,407 G→T 100% T743K (ACG→AAG)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,049,4070GT97.4% 486.9 / 3.8 154T743K (ACG→AAG) torShybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (74/76);  total (78/76)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 154 total aligned reads displayed.

ACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC  >  CP009273/1049262‑1049551
                                                                                                                                                 |                                                                                                                                                
aCCTAGCACTTCACGCGGCACGGGTTTAGGGATAATCCCGCGTAATAATGAAGTGGTACGCTGGGGCAGCGTTTAGTCTATGACATGGGCGCTAAAGCAAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTa                                                                                                                                                <  1:3390099/148‑1 (MQ=255)
     gCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGcc                                                                                                                                           <  1:608426/148‑1 (MQ=255)
      cACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCg                                                                                                                                          <  2:1347924/148‑1 (MQ=255)
      cACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCg                                                                                                                                          <  2:3114699/148‑1 (MQ=255)
      cACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCg                                                                                                                                          >  1:2195941/1‑148 (MQ=255)
       aCTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGt                                                                                                                                         <  1:295486/148‑1 (MQ=255)
       aCTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGt                                                                                                                                         <  1:294814/148‑1 (MQ=255)
       aCTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGt                                                                                                                                         <  2:2675974/148‑1 (MQ=255)
         ttCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCg                                                                                                                                       <  2:2481656/148‑1 (MQ=255)
          tCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTCATGCCGTCGa                                                                                                                                      >  2:3452290/1‑148 (MQ=255)
                ggCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCg                                                                                                                                <  1:1987902/148‑1 (MQ=255)
                ggCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCg                                                                                                                                <  1:1987728/148‑1 (MQ=255)
                     cGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGa                                                                                                                           <  1:2540504/148‑1 (MQ=255)
                       gTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCCGATc                                                                                                                         >  1:2296624/1‑148 (MQ=255)
                          tAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaaa                                                                                                                      <  1:3078268/148‑1 (MQ=255)
                          tAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCaaa                                                                                                                      <  2:2893768/148‑1 (MQ=255)
                            gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAt                                                                                                                    >  1:1719737/1‑148 (MQ=255)
                            gggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAt                                                                                                                    >  1:1470754/1‑148 (MQ=255)
                             ggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATc                                                                                                                   <  1:1712769/148‑1 (MQ=255)
                             ggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATc                                                                                                                   <  1:376162/148‑1 (MQ=255)
                             ggATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATc                                                                                                                   <  2:1645408/148‑1 (MQ=255)
                             ggATAATCCCGAGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGAAATTGTCGATAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAATCTTAATCCAGTATAAATCCGGCAGATCAAAATc                                                                                                                   >  2:1097753/1‑148 (MQ=255)
                                  aTCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATccacca                                                                                                              <  2:2796281/148‑1 (MQ=255)
                                  aTCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATccacca                                                                                                              >  2:2454020/1‑148 (MQ=255)
                                   tCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAg                                                                                                             >  1:3040563/1‑148 (MQ=255)
                                   tCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAg                                                                                                             >  1:3041404/1‑148 (MQ=255)
                                        cgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCgg                                                                                                        >  1:1668135/1‑148 (MQ=255)
                                        cgGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCgg                                                                                                        >  1:1474346/1‑148 (MQ=255)
                                           aataatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgcag                                                                                                     <  1:1083266/148‑1 (MQ=255)
                                           aataatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGgcag                                                                                                     >  2:1784371/1‑148 (MQ=255)
                                              aatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaa                                                                                                  >  2:347575/1‑148 (MQ=255)
                                              aatGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaa                                                                                                  <  1:381842/148‑1 (MQ=255)
                                               atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                 >  2:2057246/1‑148 (MQ=255)
                                               atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                 <  1:1112931/148‑1 (MQ=255)
                                               atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                 >  2:1444612/1‑148 (MQ=255)
                                               atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                 >  2:1266823/1‑148 (MQ=255)
                                               atGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCaaa                                                                                                 >  2:1266860/1‑148 (MQ=255)
                                                 gAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACg                                                                                               >  2:2018558/1‑148 (MQ=255)
                                                 gAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACg                                                                                               >  2:2018548/1‑148 (MQ=255)
                                                 gAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACg                                                                                               >  1:1707540/1‑148 (MQ=255)
                                                 gAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACg                                                                                               >  2:586998/1‑148 (MQ=255)
                                                  aaCTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACgg                                                                                              <  1:1175687/148‑1 (MQ=255)
                                                    cTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGtt                                                                                            >  2:1728053/1‑148 (MQ=255)
                                                    cTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGtt                                                                                            >  1:1799945/1‑148 (MQ=255)
                                                       gTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGc                                                                                         >  1:1555529/1‑148 (MQ=255)
                                                       gTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGc                                                                                         >  1:1800698/1‑148 (MQ=255)
                                                         aCGCTGGCGCAGCGTTTCGTAAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTa                                                                                       <  1:1874397/148‑1 (MQ=255)
                                                          cGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTAt                                                                                      <  2:2386485/148‑1 (MQ=255)
                                                               gcgcAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGc                                                                                 >  1:1942509/1‑148 (MQ=255)
                                                               gcgcAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGc                                                                                 <  1:2000244/148‑1 (MQ=255)
                                                                 gcAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAg                                                                               <  2:1235587/148‑1 (MQ=255)
                                                                  cAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAgt                                                                              <  2:1769619/148‑1 (MQ=255)
                                                                          cGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTaa                                                                      <  2:1892119/148‑1 (MQ=255)
                                                                          cGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTaa                                                                      <  2:1892034/148‑1 (MQ=255)
                                                                            tCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAg                                                                    >  1:1314824/1‑148 (MQ=255)
                                                                             cAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGc                                                                   <  1:574777/148‑1 (MQ=255)
                                                                             cAATGACATGGGCGCAAAAGCAAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGc                                                                   <  1:616284/148‑1 (MQ=255)
                                                                              aaTGACTTGGGCGCAAACGCCAATCAAAACCCGCGTCGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATTTCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGcc                                                                  <  1:1835172/148‑1 (MQ=255)
                                                                              aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGcc                                                                  >  1:1207831/1‑148 (MQ=255)
                                                                              aaTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGcc                                                                  <  1:504752/148‑1 (MQ=255)
                                                                               aTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCt                                                                 >  2:1783324/1‑148 (MQ=255)
                                                                               aTGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCt                                                                 >  2:2312402/1‑148 (MQ=255)
                                                                                tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                >  2:886643/1‑148 (MQ=255)
                                                                                tGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTg                                                                >  2:414613/1‑148 (MQ=255)
                                                                                  aCATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                              >  2:3317835/1‑148 (MQ=255)
                                                                                  aCATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTgcg                                                              <  2:3097743/148‑1 (MQ=255)
                                                                                    aTGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGcgcg                                                            >  2:1899574/1‑148 (MQ=255)
                                                                                    aTGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGcgcg                                                            >  1:3077952/1‑148 (MQ=255)
                                                                                     tGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCgg                                                           >  1:2445872/1‑148 (MQ=255)
                                                                                       ggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCa                                                         <  1:2078007/148‑1 (MQ=255)
                                                                                       ggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCa                                                         >  2:1526198/1‑148 (MQ=255)
                                                                                       ggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCa                                                         >  2:491534/1‑148 (MQ=255)
                                                                                       ggCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCa                                                         >  2:491442/1‑148 (MQ=255)
                                                                                        gcgcTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCAt                                                        <  1:2754438/148‑1 (MQ=255)
                                                                                           cTAAAGCCAATCAACACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGc                                                     <  1:1614661/148‑1 (MQ=255)
                                                                                           cTAAAGCCAATCAAAACCCGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGc                                                     <  2:62704/148‑1 (MQ=255)
                                                                                           cTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGc                                                     >  2:434734/1‑148 (MQ=255)
                                                                                           cTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGc                                                     <  1:1613409/148‑1 (MQ=255)
                                                                                             aaaGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCa                                                   >  2:2697617/1‑148 (MQ=255)
                                                                                               aGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAc                                                 >  1:1121674/1‑148 (MQ=255)
                                                                                                      cAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaaca                                          >  2:2419026/1‑148 (MQ=255)
                                                                                                      cAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGcaaca                                          <  2:1189138/148‑1 (MQ=255)
                                                                                                         aaCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATc                                       >  2:3042459/1‑148 (MQ=255)
                                                                                                           ccAGCGACGGATATTGCTGTGCCAGTTGTCGGGCCAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCAACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTg                                     >  2:3360572/1‑148 (MQ=255)
                                                                                                           ccAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTg                                     >  2:3360581/1‑148 (MQ=255)
                                                                                                                gACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcac                                <  1:231237/148‑1 (MQ=255)
                                                                                                                 aCGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacc                               <  2:2902204/148‑1 (MQ=255)
                                                                                                                  cGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcacca                              <  2:2684426/148‑1 (MQ=255)
                                                                                                                   ggATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGcaccac                             <  1:428913/148‑1 (MQ=255)
                                                                                                                    gATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACt                            <  1:783522/148‑1 (MQ=255)
                                                                                                                    gATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACt                            >  2:2369954/1‑148 (MQ=255)
                                                                                                                          gCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGtttt                      <  1:832498/148‑1 (MQ=255)
                                                                                                                               gCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCa                 <  1:1073520/148‑1 (MQ=255)
                                                                                                                               gCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCa                 <  1:2854434/148‑1 (MQ=255)
                                                                                                                                  aGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCAtct              >  2:1481140/1‑148 (MQ=255)
                                                                                                                                    ttGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCa            >  2:1097427/1‑148 (MQ=255)
                                                                                                                                      gTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAAt          <  1:472230/148‑1 (MQ=255)
                                                                                                                                       tCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATg         >  2:2660664/1‑148 (MQ=255)
                                                                                                                                            cAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTact    >  2:1923360/1‑146 (MQ=255)
                                                                                                                                              aGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTc  >  1:86945/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC  >  CP009273/1049262‑1049551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGC  >  CP009273/1049258‑1049553
                                                                                                                                                     |                                                                                                                                                  
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCT                                                                                                                                                    <  SRR19973066.2468619/150‑1 (MQ=60)
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCT                                                                                                                                                    <  SRR19973066.1661312/150‑1 (MQ=60)
 CTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCTGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAACCCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTT                                                                                                                                                   <  SRR19973066.1650550/150‑1 (MQ=60)
 CTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTT                                                                                                                                                   <  SRR19973066.1650607/150‑1 (MQ=60)
  TGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTA                                                                                                                                                  <  SRR19973066.1443477/150‑1 (MQ=60)
  TGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTA                                                                                                                                                  <  SRR19973066.1443616/150‑1 (MQ=60)
    ACCTAGCACTTCACGCGGCACGGGTTTAGGGATAATCCCGCGTAATAATGAAGTGGTACGCTGGGGCAGCGTTTAGTCTATGACATGGGCGCTAAAGCAAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAAT                                                                                                                                                <  SRR19973066.3412725/150‑1 (MQ=60)
        AGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCG                                                                                                                                            >  SRR19973066.2211169/1‑150 (MQ=60)
         GCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGT                                                                                                                                           <  SRR19973066.3066277/150‑1 (MQ=60)
         GCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGT                                                                                                                                           <  SRR19973066.612651/150‑1 (MQ=60)
           ACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCG                                                                                                                                         <  SRR19973066.297586/150‑1 (MQ=60)
           ACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCG                                                                                                                                         <  SRR19973066.296912/150‑1 (MQ=60)
                 CGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCC                                                                                                                                   >  SRR19973066.385937/1‑150 (MQ=60)
                  GCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCG                                                                                                                                  <  SRR19973066.2958376/150‑1 (MQ=60)
                    GGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGC                                                                                                                                <  SRR19973066.2001736/150‑1 (MQ=60)
                    GGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGC                                                                                                                                <  SRR19973066.2001910/150‑1 (MQ=60)
                         CGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTCAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATC                                                                                                                           >  SRR19973066.2311492/1‑150 (MQ=60)
                         CGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCCGATC                                                                                                                           >  SRR19973066.2312370/1‑150 (MQ=60)
                         CGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATC                                                                                                                           <  SRR19973066.2182433/150‑1 (MQ=60)
                         CGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATC                                                                                                                           <  SRR19973066.2557767/150‑1 (MQ=60)
                         CGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATC                                                                                                                           <  SRR19973066.2421013/150‑1 (MQ=60)
                              TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                      >  SRR19973066.1481237/1‑150 (MQ=60)
                              TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                      >  SRR19973066.1481127/1‑150 (MQ=60)
                              TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                      <  SRR19973066.3098832/150‑1 (MQ=60)
                              TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                      >  SRR19973066.1732189/1‑150 (MQ=60)
                              TAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAAT                                                                                                                      >  SRR19973066.1732176/1‑150 (MQ=60)
                                 GGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCA                                                                                                                   <  SRR19973066.379116/150‑1 (MQ=60)
                                 GGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCA                                                                                                                   <  SRR19973066.1725190/150‑1 (MQ=60)
                                   ATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACC                                                                                                                 >  SRR19973066.3000671/1‑150 (MQ=60)
                                   ATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACC                                                                                                                 >  SRR19973066.375663/1‑150 (MQ=60)
                                     AATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAG                                                                                                               >  SRR19973066.3061732/1‑150 (MQ=60)
                                     AATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAG                                                                                                               >  SRR19973066.3060882/1‑150 (MQ=60)
                                          CGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGG                                                                                                          >  SRR19973066.1681570/1‑150 (MQ=60)
                                          CGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGG                                                                                                          >  SRR19973066.1680289/1‑150 (MQ=60)
                                          CGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGG                                                                                                          >  SRR19973066.1484731/1‑150 (MQ=60)
                                               AATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCA                                                                                                     <  SRR19973066.1091176/150‑1 (MQ=60)
                                               AATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCA                                                                                                     <  SRR19973066.1091201/150‑1 (MQ=60)
                                                  AATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAAC                                                                                                  <  SRR19973066.384857/150‑1 (MQ=60)
                                                   ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                 <  SRR19973066.1120972/150‑1 (MQ=60)
                                                   ATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACG                                                                                                 >  SRR19973066.1719931/1‑150 (MQ=60)
                                                      AACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTT                                                                                              <  SRR19973066.1183971/150‑1 (MQ=60)
                                                      AACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTT                                                                                              >  SRR19973066.1812738/1‑150 (MQ=60)
                                                      AACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTT                                                                                              >  SRR19973066.1812983/1‑150 (MQ=60)
                                                         TGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGC                                                                                           >  SRR19973066.1566713/1‑150 (MQ=60)
                                                         TGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGC                                                                                           >  SRR19973066.1568071/1‑150 (MQ=60)
                                                         TGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGC                                                                                           >  SRR19973066.1813495/1‑150 (MQ=60)
                                                             ACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATT                                                                                       <  SRR19973066.1887775/150‑1 (MQ=60)
                                                             ACGCTGGCGCAGCGTTTCGTAAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATT                                                                                       <  SRR19973066.1887776/150‑1 (MQ=60)
                                                              CGCTGGCGCAGCGTTTCGTCAATGACAGGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTT                                                                                      <  SRR19973066.1673955/150‑1 (MQ=60)
                                                                 TGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGC                                                                                   >  SRR19973066.1956280/1‑150 (MQ=60)
                                                                   GCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAG                                                                                 <  SRR19973066.2014302/150‑1 (MQ=60)
                                                                      CAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGT                                                                              >  SRR19973066.495103/1‑150 (MQ=60)
                                                                         CGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTC                                                                           <  SRR19973066.2618188/150‑1 (MQ=60)
                                                                              CGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAG                                                                      >  SRR19973066.1163682/1‑150 (MQ=60)
                                                                              CGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAG                                                                      >  SRR19973066.1324358/1‑150 (MQ=60)
                                                                                TCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCC                                                                    >  SRR19973066.1216265/1‑150 (MQ=60)
                                                                                 CAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCT                                                                   <  SRR19973066.578826/150‑1 (MQ=60)
                                                                                 CAATGACATGGGCGCAAAAGCAAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATAAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCT                                                                   <  SRR19973066.620618/150‑1 (MQ=60)
                                                                                 CAATGACAGGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCT                                                                   <  SRR19973066.578909/150‑1 (MQ=60)
                                                                                  AATGACTTGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                  <  SRR19973066.2006984/150‑1 (MQ=60)
                                                                                  AATGACTTGGGCGCAAACGCCAATCAAAACCCGCGTCGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATTTCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                  <  SRR19973066.1848279/150‑1 (MQ=60)
                                                                                  AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                  <  SRR19973066.196563/150‑1 (MQ=60)
                                                                                  AATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTG                                                                  <  SRR19973066.508503/150‑1 (MQ=60)
                                                                                      ACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCG                                                              >  SRR19973066.3098511/1‑150 (MQ=60)
                                                                                       CATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGG                                                             >  SRR19973066.2462533/1‑150 (MQ=60)
                                                                                           GGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATT                                                         <  SRR19973066.2092366/150‑1 (MQ=60)
                                                                                            GCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTG                                                        <  SRR19973066.2772984/150‑1 (MQ=60)
                                                                                               CTAAAGCCAATCAACACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCA                                                     <  SRR19973066.1626375/150‑1 (MQ=60)
                                                                                               CTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCA                                                     <  SRR19973066.1625109/150‑1 (MQ=60)
                                                                                                 AAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAC                                                   >  SRR19973066.3230825/1‑150 (MQ=60)
                                                                                                 AAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAAC                                                   >  SRR19973066.1129756/1‑150 (MQ=60)
                                                                                                  AAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                  >  SRR19973066.1312899/1‑150 (MQ=60)
                                                                                                  AAGCCAAACAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACA                                                  <  SRR19973066.710481/150‑1 (MQ=60)
                                                                                                                    GACGGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCA                                <  SRR19973066.233072/150‑1 (MQ=60)
                                                                                                                       GGATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTG                             <  SRR19973066.432229/150‑1 (MQ=60)
                                                                                                                        GATATTGCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGG                            <  SRR19973066.789307/150‑1 (MQ=60)
                                                                                                                              GCTGTGCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCA                      <  SRR19973066.838567/150‑1 (MQ=60)
                                                                                                                                   GCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATC                 <  SRR19973066.2873613/150‑1 (MQ=60)
                                                                                                                                   GCCAGTTGTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATC                 <  SRR19973066.1081382/150‑1 (MQ=60)
                                                                                                                                          GTCGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGG          <  SRR19973066.475810/150‑1 (MQ=60)
                                                                                                                                            CGGGCAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTA        >  SRR19973066.86283/1‑150 (MQ=60)
                                                                                                                                                CAAGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTC    >  SRR19973066.87894/1‑150 (MQ=60)
                                                                                                                                                  AGCTTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGC  >  SRR19973066.2129236/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
ACTGACCGAGCACTTCACGCGGCACCGGTTTAGGGATAATCCCGCGGAATAATGAACTGGTACGCTGGCGCAGCGTTTCGTCAATGACATGGGCGCTAAAGCCAATCAAAACCAGCGACGGATATTGCTGTGCCAGTTGTCGGGCAAGCGTAATGCCGTCGATATCCGGCAGATCAAAATCCACCAGTGCGGCAGCAAACGGTTCGCTATTTTGCAGTGTCTCTAAAGCCTGCGCGGCATTGCCAACAGCAACAATCTGCGCACCACTGGTTTTCAGCATCTCAATGGTAATTCGC  >  CP009273/1049258‑1049553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑