Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,860,159 C→A 87.9% P33P (CCG→CCT)  ibpB ← heat shock chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,860,1590CA87.9% 458.4 / 67.3 221P33P (CCG→CCT) ibpBheat shock chaperone
Reads supporting (aligned to +/- strand):  ref base C (13/13);  new base A (86/102);  total (106/115)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

Only 100 of 228 total aligned reads displayed.

TTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860012‑3860308
                                                                                                                                                   |                                                                                                                                                     
ttttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAcggagg                                                                                                                                                     <  2:2538672/150‑1 (MQ=255)
 tttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTGTAAGGAggg                                                                                                                                                    >  1:1332086/1‑150 (MQ=255)
  ttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGa                                                                                                                                                   <  1:2536073/150‑1 (MQ=255)
   tttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCCGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGaa                                                                                                                                                  <  2:1765989/150‑1 (MQ=255)
     tctcTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGc                                                                                                                                                <  1:3378869/150‑1 (MQ=255)
      ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGct                                                                                                                                               >  1:3301899/1‑150 (MQ=255)
       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTGTAAGGAGGGTAGcgc                                                                                                                                              >  1:3948440/1‑148 (MQ=255)
       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGctc                                                                                                                                              <  1:3023049/150‑1 (MQ=255)
       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTATAAATTTTTTTCGGAGGGAAGctc                                                                                                                                              >  1:531371/1‑150 (MQ=255)
           cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTTTTAGGAGGGACGCGCTggg                                                                                                                                          >  1:2075013/1‑149 (MQ=255)
            ttttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCt                                                                                                                                         <  2:4472079/150‑1 (MQ=255)
            ttttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGCTCTGGCt                                                                                                                                         >  2:457370/1‑150 (MQ=255)
              ttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCttt                                                                                                                                       <  1:4506680/150‑1 (MQ=255)
              ttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCttt                                                                                                                                       <  1:3136775/150‑1 (MQ=255)
               tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCttta                                                                                                                                      >  2:4325236/1‑149 (MQ=255)
                   tgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCCATGTTGTAAGGAGGGAAGCTCTGGCTTGAACCg                                                                                                                                  >  1:2793364/1‑150 (MQ=255)
                     ctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGGTGTTCGGATGGAAGCACTTGCGTTCACCGGc                                                                                                                                >  2:1130208/1‑150 (MQ=255)
                        cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                             <  1:2925839/150‑1 (MQ=255)
                        cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                             <  2:59613/150‑1 (MQ=255)
                         ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAATTTGTTAGGTGGGAAGCTCTGGCTTTAAACGGCGgtt                                                                                                                            >  2:1194142/1‑150 (MQ=255)
                           cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                          <  2:3626330/150‑1 (MQ=255)
                             tGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCa                                                                                                                        >  2:2933446/1‑150 (MQ=255)
                             tGCCTTTGACGCTCAGGCGCGGACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCa                                                                                                                        <  2:3980324/150‑1 (MQ=255)
                              gCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTTTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAg                                                                                                                       >  2:1646112/1‑150 (MQ=255)
                               ccTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTAAGGAGGGAAGCTCTGGCTTTAAACGGCGGTTTGCAGt                                                                                                                      >  2:2761561/1‑150 (MQ=255)
                                cTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTg                                                                                                                     >  2:4253653/1‑150 (MQ=255)
                                    tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGtt                                                                                                                 <  2:2936896/150‑1 (MQ=255)
                                       gCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGc                                                                                                              <  1:1338460/150‑1 (MQ=255)
                                        cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGcc                                                                                                             <  2:967736/150‑1 (MQ=255)
                                         tCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTTCGGAGGGTAGCGCTGGGTTTAACCGGCGTTGTGAAGGGCGTTGGCCa                                                                                                            >  2:2152757/1‑150 (MQ=255)
                                           aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTAAATTTTTTACGGAGGGAAGCTCTGGCGTTCACCGGCGGGTTTCACTGCGTTGGccccg                                                                                                          >  1:2462861/1‑147 (MQ=255)
                                           aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGt                                                                                                          <  1:4011388/150‑1 (MQ=255)
                                           aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGt                                                                                                          <  1:3663273/150‑1 (MQ=255)
                                            ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTAAGGTGGGAAGCTCTGGGTTTGACCGGCGGTTTTCAGTGCGTTGGCCAGtt                                                                                                         >  1:2973206/1‑150 (MQ=255)
                                               gcgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGt                                                                                                      <  1:4144232/150‑1 (MQ=255)
                                                cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                     <  1:3759790/150‑1 (MQ=255)
                                                cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                     <  1:3595705/150‑1 (MQ=255)
                                                  tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGGTTTTCGGAGGGAACCGCTGGCTTTCACCCGCCGGTTGCAGTGCGGGTGGCAGTTTGTGaa                                                                                                   >  1:1312985/1‑150 (MQ=255)
                                                  tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAAGGAGGGAAGATCTGGCGTTAAACGGCGGTTTTCAGTGCGTTGGCCAGGTTGTCaa                                                                                                   >  2:644638/1‑150 (MQ=255)
                                                     cTTCCAGTTGAATCTCTAAATATTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTTTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTGTAaaaaa                                                                                                >  2:1781238/1‑149 (MQ=255)
                                                      ttCCAGTTGAATCTCTAACTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAcc                                                                                               <  2:1145427/150‑1 (MQ=255)
                                                       tCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGGTGTACGGAGGGAAGCGCTGGCGTTAACCGGCGTTTTGCAGTGCGTTTGCCAGTTTGTCaaaaacc                                                                                              >  2:4291185/1‑147 (MQ=255)
                                                          aGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCATGTGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAACCCGATc                                                                                           <  1:983501/150‑1 (MQ=255)
                                                             tGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAt                                                                                        <  1:4460563/150‑1 (MQ=255)
                                                                aTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGGAGCTCTGGCTTTCAACGGCGGTTTTCAGTGCGGTTGCCACGTTGTCAAAACCCATACAATGa                                                                                     >  1:3746900/1‑150 (MQ=255)
                                                                 tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAc                                                                                    <  1:143742/150‑1 (MQ=255)
                                                                  ctctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACg                                                                                   <  1:3921707/150‑1 (MQ=255)
                                                                  ctctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACg                                                                                   <  1:331231/150‑1 (MQ=255)
                                                                    ctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTAAGGAGGGTAGGTCTGGCTTTGACCGGCGGTTTTGAGTGCGGTGGGCAGGGTGTGAAAAACCATACAttttcgcc                                                                                 >  1:601100/1‑144 (MQ=255)
                                                                       aaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCa                                                                              <  1:1039953/150‑1 (MQ=255)
                                                                        aTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTTTGCCCGTTTGTCAAAACCGATCCAATGACGCATCAg                                                                             >  2:1023001/1‑150 (MQ=255)
                                                                          cTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGGTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTTTCAAAACCGATCCATTGACGCATCAGTg                                                                           >  2:2948912/1‑150 (MQ=255)
                                                                           ttCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCAAGTTTTAAGGCGGGCAGCTCAGGCTTTCACCCGCGGTTTGCAGTGCGGTGGGCAGGTTTTCAAAAACCAAACAAAGACGCCTCAGTgg                                                                          >  2:1196434/1‑150 (MQ=255)
                                                                            tCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTggg                                                                         <  2:2379561/150‑1 (MQ=255)
                                                                             ccTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTGTCAAAACCGATACATTGACGCATCAGTgggg                                                                        >  2:3272127/1‑150 (MQ=255)
                                                                                 aCGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATaa                                                                    <  2:2387746/150‑1 (MQ=255)
                                                                                 aCGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATaa                                                                    <  2:1713894/150‑1 (MQ=255)
                                                                                  cGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCGGCATGTTGGACCGGGGGTAGCTCTGGCGTTCACCGGCGTTTTGCAGTGCGGTGGCCAGGTTGTCCAAAACGCTACCTTGGCGCAGCCggggggctcaa                                                                   >  2:3099325/1‑145 (MQ=255)
                                                                                   ggAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGTGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGTTTGTCAAAACCGATACATTGTCGCCACAGGGGGGATAAAt                                                                  >  2:4144109/1‑150 (MQ=255)
                                                                                    gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                                 <  2:131381/150‑1 (MQ=255)
                                                                                    gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                                 <  2:4178858/150‑1 (MQ=255)
                                                                                       aCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTATCAAAGTTGTACGGAGGGAAGCACAGGCGGTCACCCGCGGTTTGCAGTGCGGTGGGCCCGTTGTCAAAACCCAACCATTGACGCCTCAGGGGGGGTAAATAGaa                                                              >  2:450428/1‑150 (MQ=255)
                                                                                         cTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCGTTCACCGGCGGTTTTCAGTTCGTTGGCCAGTTTTTCAAAAACGATCCAATGAAGCATCAGGGGGGTTAAATCGAAGt                                                            >  2:3021549/1‑150 (MQ=255)
                                                                                          tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGtt                                                           <  2:4350877/150‑1 (MQ=255)
                                                                                              aGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGGTGGCCAGGTTGTCAAAAACGATACATTGACGCATCCGTGGGGATAAATAGAAGTTAAGc                                                       >  2:3659681/1‑150 (MQ=255)
                                                                                              aGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTTTAAGGAGGGAAGCTCTGGGTTTAACCGGCGTTTTTCAGTGCGTTTGCCAGTTTTTCAAAAACCATCCATTTACGCATCAGTGGGGGTAAATCGAAGTTAAGc                                                       >  2:2867846/1‑150 (MQ=255)
                                                                                                cgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGGTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCAt                                                     <  2:2948299/150‑1 (MQ=255)
                                                                                                  cAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAg                                                   <  1:1567799/150‑1 (MQ=255)
                                                                                                   aaGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGt                                                  <  1:207091/150‑1 (MQ=255)
                                                                                                      ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTTTAAGGAGGGAAGCTCTGGCTTTCAACGGCGTTTTTCAGTGGGTTGGGCAGTTTGTCAAAAACGATCCATTGAAGCAAAAGTGGGGTTAAATAGAAGTTAAGCAAAGtaaa                                               >  2:1352767/1‑147 (MQ=255)
                                                                                                      ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAt                                               <  1:2479458/150‑1 (MQ=255)
                                                                                                      ggTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTTTACCGAGGGAAGCTCTGGCTTTCACCCGCGGTTTGCCGTGCGTTGGCCCGTTTGTGAAAAACGATCCAATTACGCATCAGTGGGGATAAAACGAAGTTTAGCAAAGTAAt                                               >  2:3342195/1‑150 (MQ=255)
                                                                                                       gTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCAGGCGTTCACCGGCGTTTTGCAGTGCCTTGGCCCGTTTGTCAAAACCGATCCCTTGACGCCTCACTGGGGATAAAACGAAGTTACCCAAAGTCaat                                              >  2:2219274/1‑148 (MQ=255)
                                                                                                        tAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAttt                                             <  2:4231378/150‑1 (MQ=255)
                                                                                                        tAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAttt                                             <  1:1194131/150‑1 (MQ=255)
                                                                                                         aaTGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTtc                                            <  2:1839098/150‑1 (MQ=255)
                                                                                                            gCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTcc                                         <  2:4152852/150‑1 (MQ=255)
                                                                                                              ggTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCtt                                       <  2:2441095/150‑1 (MQ=255)
                                                                                                                  gTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaa                                   <  1:3988925/150‑1 (MQ=255)
                                                                                                                  gTGGTTATCGTCGCTTTTCTAAATTTTGTAAGGAGGGAATCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTTTGCCAGTTTTTAAAAAACGATCCATTTACCCATCAGTGGGGAAAAAAAGAAGTTACGCATAGTCATTTATCCTTCTaa                                   >  2:2568138/1‑150 (MQ=255)
                                                                                                                     gTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaagaa                                <  1:4157822/150‑1 (MQ=255)
                                                                                                                          cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGag                           >  2:1827914/1‑150 (MQ=255)
                                                                                                                          cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGag                           <  2:948537/150‑1 (MQ=255)
                                                                                                                          cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAACGATACATTTAAGCATCAGTGGGGATAAATAGAAGTTACCCAAACTCAATTATACATCTAAGAAGCGag                           >  2:3210757/1‑150 (MQ=255)
                                                                                                                           gtcgCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAAGCATCAGTGGGGATAAATAGAAGTTAAGCATAGTCATTTATCCTTCTAATAAGCGagt                          >  2:844848/1‑150 (MQ=255)
                                                                                                                           gtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagt                          >  2:3563650/1‑150 (MQ=255)
                                                                                                                              gCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCATTCTAAGAATCGagtaag                       >  2:3688466/1‑150 (MQ=255)
                                                                                                                              gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                       <  2:3437320/150‑1 (MQ=255)
                                                                                                                              gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                       >  1:2570798/1‑150 (MQ=255)
                                                                                                                               cTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagt                      >  1:4159259/1‑150 (MQ=255)
                                                                                                                                  ttCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                   >  1:1071875/1‑150 (MQ=255)
                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  1:3282686/150‑1 (MQ=255)
                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  2:263524/150‑1 (MQ=255)
                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  1:1005113/150‑1 (MQ=255)
                                                                                                                                    ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg                 <  1:3255858/150‑1 (MQ=255)
                                                                                                                                           ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc          <  2:736178/150‑1 (MQ=255)
                                                                                                                                           ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc          <  2:1029948/150‑1 (MQ=255)
                                                                                                                                           ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc          <  2:1795888/150‑1 (MQ=255)
                                                                                                                                                cggcggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCCTAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGa     >  2:783187/1‑150 (MQ=255)
                                                                                                                                                cggaggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGa     >  2:3481171/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860012‑3860308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860010‑3860308
                                                                                                                                                     |                                                                                                                                                     
CATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGA                                                                                                                                                       <  SRR19973080.2829753/150‑1 (MQ=60)
 ATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAG                                                                                                                                                      <  SRR19973080.2987966/150‑1 (MQ=60)
   TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGG                                                                                                                                                    <  SRR19973080.1056867/150‑1 (MQ=60)
   TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTGTAAGGAGGG                                                                                                                                                    >  SRR19973080.1340116/1‑150 (MQ=60)
   TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGG                                                                                                                                                    >  SRR19973080.2558300/1‑150 (MQ=60)
    TTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGA                                                                                                                                                   <  SRR19973080.2549991/150‑1 (MQ=60)
     TTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTTTAAGGAGGGAA                                                                                                                                                  >  SRR19973080.4164371/1‑150 (MQ=60)
       TCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGC                                                                                                                                                <  SRR19973080.3397157/150‑1 (MQ=60)
       TCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGAAGC                                                                                                                                                >  SRR19973080.3034410/1‑150 (MQ=60)
        CTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCT                                                                                                                                               >  SRR19973080.3319967/1‑150 (MQ=60)
        CTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGCT                                                                                                                                               >  SRR19973080.3319972/1‑150 (MQ=60)
        CTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCT                                                                                                                                               >  SRR19973080.3322458/1‑150 (MQ=60)
         TCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTC                                                                                                                                              <  SRR19973080.3039270/150‑1 (MQ=60)
         TCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTC                                                                                                                                              >  SRR19973080.2454620/1‑150 (MQ=60)
                TTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTT                                                                                                                                       <  SRR19973080.3154041/150‑1 (MQ=60)
                TTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTT                                                                                                                                       <  SRR19973080.4530470/150‑1 (MQ=60)
                TTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTT                                                                                                                                       <  SRR19973080.4531013/150‑1 (MQ=60)
                   GCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTTAGGAGGGAAGCGCTGGGTTTCAC                                                                                                                                    >  SRR19973080.1622579/1‑150 (MQ=60)
                     TGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCG                                                                                                                                  <  SRR19973080.2180045/150‑1 (MQ=60)
                     TGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCCATGTTGTAAGGAGGGAAGCTCTGGCTTGAACCG                                                                                                                                  >  SRR19973080.2808764/1‑150 (MQ=60)
                        TCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCG                                                                                                                               <  SRR19973080.3243274/150‑1 (MQ=60)
                          CGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTT                                                                                                                             <  SRR19973080.2941761/150‑1 (MQ=60)
                          CGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTT                                                                                                                             <  SRR19973080.2941587/150‑1 (MQ=60)
                            GCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTT                                                                                                                           <  SRR19973080.3305451/150‑1 (MQ=60)
                                      TACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT                                                                                                                 <  SRR19973080.3737494/150‑1 (MQ=60)
                                         GCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGC                                                                                                              <  SRR19973080.1346520/150‑1 (MQ=60)
                                             AGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTAAATTTTTTACGGAGGGAAGCTCTGGCGTTCACCGGCGggtttcactgcgttggccccg                                                                                                          >  SRR19973080.2476493/1‑129 (MQ=60)
                                             AGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGT                                                                                                          <  SRR19973080.3683461/150‑1 (MQ=60)
                                             AGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGT                                                                                                          <  SRR19973080.3683463/150‑1 (MQ=60)
                                             AGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGT                                                                                                          <  SRR19973080.4033404/150‑1 (MQ=60)
                                             AGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTACGGGGGGAAGCTCTGGCTTTAAACCGCGTTTTGCAGTGCGGTGGGCAGT                                                                                                          >  SRR19973080.2476498/1‑150 (MQ=60)
                                              GGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGTAGCTCTGGGTTTGACCGGCGgttttgagttgggttgccagtt                                                                                                         >  SRR19973080.2989287/1‑128 (MQ=60)
                                              GGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTAAGGTGGGAAGCTCTGGGTTTGACCGGCGGTTTTCAGTGCGTTGGCCAGTT                                                                                                         >  SRR19973080.2989279/1‑150 (MQ=60)
                                                 GCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGT                                                                                                      <  SRR19973080.4166634/150‑1 (MQ=60)
                                                 GCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGT                                                                                                      <  SRR19973080.4166667/150‑1 (MQ=60)
                                                  CGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTC                                                                                                     <  SRR19973080.3780284/150‑1 (MQ=60)
                                                  CGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTC                                                                                                     <  SRR19973080.3780254/150‑1 (MQ=60)
                                                  CGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTC                                                                                                     <  SRR19973080.3615577/150‑1 (MQ=60)
                                                       CTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAC                                                                                                <  SRR19973080.1414439/150‑1 (MQ=60)
                                                         TCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCG                                                                                              <  SRR19973080.444486/150‑1 (MQ=60)
                                                            AGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCATGTGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAACCCGATC                                                                                           <  SRR19973080.989437/150‑1 (MQ=60)
                                                            AGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATC                                                                                           <  SRR19973080.3153110/150‑1 (MQ=60)
                                                               TGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAT                                                                                        <  SRR19973080.4484736/150‑1 (MQ=60)
                                                               TGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAT                                                                                        <  SRR19973080.4484122/150‑1 (MQ=60)
                                                                  ATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGGAGCTCTGGCTTTCAACGGCGGTTTTCAGTGCGgttgccacgttgtcaaaacccatacaatga                                                                                     >  SRR19973080.3767368/1‑120 (MQ=60)
                                                                   TCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAC                                                                                    <  SRR19973080.144925/150‑1 (MQ=60)
                                                                    CTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACG                                                                                   <  SRR19973080.3943362/150‑1 (MQ=60)
                                                                    CTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACG                                                                                   <  SRR19973080.333239/150‑1 (MQ=60)
                                                                      CTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTGTACGGAGGGAAGCGCTGGCGTTCACCCGCGGGTTGGAGTGCGTTGGGCAGTTTGTCAAAAACCATCCAGTGACGCC                                                                                 >  SRR19973080.604842/1‑150 (MQ=60)
                                                                      CTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTTAGGAGGGGAGCACTGGCTTTAACCGGCGGTTTTCAGTGGGGTGGCCAGTTTGTgaaaaaccatccaaatacgca                                                                                 >  SRR19973080.2879652/1‑129 (MQ=60)
                                                                      CTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTAAGGAGGGTAGGTCTGGCTTTGACCGGCGgttttgagtgcggtgggcagggtgtgaaaaaccatacattttcgcc                                                                                 >  SRR19973080.604819/1‑104 (MQ=60)
                                                                       TAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCAT                                                                                <  SRR19973080.1339243/150‑1 (MQ=60)
                                                                         AATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCA                                                                              <  SRR19973080.1045602/150‑1 (MQ=60)
                                                                         AATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCA                                                                              <  SRR19973080.1046097/150‑1 (MQ=60)
                                                                                CTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGCGGGAAGCTATGGCGTTCACCGGggggttggcgggcgttgggccgtttgtcaaaacccaacccattacgcagccggggggg                                                                       >  SRR19973080.636046/1‑92 (MQ=60)
                                                                                  GACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATA                                                                     <  SRR19973080.3520890/150‑1 (MQ=60)
                                                                                   ACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTACGGTGGGAAGCGCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCcggttgtgaaaaaccagcccatgacgcaacaggggggataa                                                                    >  SRR19973080.3377906/1‑109 (MQ=60)
                                                                                          CCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAG                                                             <  SRR19973080.1280130/150‑1 (MQ=60)
                                                                                           CTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGT                                                            <  SRR19973080.2505284/150‑1 (MQ=60)
                                                                                              CCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTGTTAGGAGGGTAGCTCTGGCGTTCACCCGCGTTTTTGAGTGCGTTGGCCAGTTTGTCAAAAACGAACCATTTACGCATAAGTGGGGgtaaaaagaatttaa                                                         >  SRR19973080.1489568/1‑135 (MQ=60)
                                                                                              CCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTTAGGAGGGAAGCTCTGGCGTTCACCGGCGGTTTGCAGTTCGGTGGCCAGgttttcaaaaaccctcccatgtcgcctcagggggggtaaaaagaagttaa                                                         >  SRR19973080.4508488/1‑100 (MQ=60)
                                                                                                    CAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAG                                                   <  SRR19973080.1576939/150‑1 (MQ=60)
                                                                                                    CAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAG                                                   <  SRR19973080.1576879/150‑1 (MQ=60)
                                                                                                     AAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGT                                                  <  SRR19973080.208626/150‑1 (MQ=60)
                                                                                                     AAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTATGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGT                                                  <  SRR19973080.208644/150‑1 (MQ=60)
                                                                                                        GGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAT                                               <  SRR19973080.2493169/150‑1 (MQ=60)
                                                                                                          TAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTT                                             <  SRR19973080.4022455/150‑1 (MQ=60)
                                                                                                          TAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTT                                             <  SRR19973080.1201318/150‑1 (MQ=60)
                                                                                                          TAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTT                                             <  SRR19973080.1201329/150‑1 (MQ=60)
                                                                                                            ATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCT                                           <  SRR19973080.2776796/150‑1 (MQ=60)
                                                                                                                GGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTT                                       <  SRR19973080.3960733/150‑1 (MQ=60)
                                                                                                                   AGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTA                                    <  SRR19973080.775072/150‑1 (MQ=60)
                                                                                                                    GTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAA                                   <  SRR19973080.4010863/150‑1 (MQ=60)
                                                                                                                       GTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAA                                <  SRR19973080.4180263/150‑1 (MQ=60)
                                                                                                                                GCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAG                       >  SRR19973080.2584835/1‑150 (MQ=60)
                                                                                                                                 CTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGT                      >  SRR19973080.4181212/1‑150 (MQ=60)
                                                                                                                                 CTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGT                      >  SRR19973080.4181702/1‑150 (MQ=60)
                                                                                                                                 CTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCCATACATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATATTCATTTATCCTTATAAGAATCGAGTAAGT                      >  SRR19973080.3489924/1‑150 (MQ=60)
                                                                                                                                    TTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAATCGAGTAAGTACC                   >  SRR19973080.1078112/1‑150 (MQ=60)
                                                                                                                                     TCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCT                  <  SRR19973080.1011132/150‑1 (MQ=60)
                                                                                                                                     TCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCT                  <  SRR19973080.3300644/150‑1 (MQ=60)
                                                                                                                                     TCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCT                  <  SRR19973080.1085780/150‑1 (MQ=60)
                                                                                                                                     TCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCT                  <  SRR19973080.3300711/150‑1 (MQ=60)
                                                                                                                                      CTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTG                 <  SRR19973080.3273772/150‑1 (MQ=60)
                                                                                                                                              TGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCG         <  SRR19973080.2957642/150‑1 (MQ=60)
                                                                                                                                              TGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGAGCGGTGGCCAGATTGTAAAAACCCAACCAATGACGCAACAGTGGGGATAAAACGAAGGTAAGCAAAGTCATTTCTACATCTAAGAAACGCGTAAGTACCAGCAAATCCC         >  SRR19973080.3822439/1‑150 (MQ=51)
                                                                                                                                                   GGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGAT    <  SRR19973080.2315222/150‑1 (MQ=60)
                                                                                                                                                    GAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATT   <  SRR19973080.3315762/150‑1 (MQ=60)
                                                                                                                                                    GAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATT   <  SRR19973080.3316083/150‑1 (MQ=60)
                                                                                                                                                     CGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGTTTGTCAAAACCGATCCAATGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCACCTTCTAAGAAGCGAGTAAGTACCAGCAAAACCGAAGATTC  >  SRR19973080.2773311/1‑150 (MQ=60)
                                                                                                                                                     AGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  <  SRR19973080.983466/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860010‑3860308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑