Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,860,159 C→A 93.7% P33P (CCG→CCT)  ibpB ← heat shock chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,860,1590CA93.7% 604.5 / 28.7 230P33P (CCG→CCT) ibpBheat shock chaperone
Reads supporting (aligned to +/- strand):  ref base C (5/10);  new base A (94/115);  total (105/125)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

Only 100 of 233 total aligned reads displayed.

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGA  >  CP009273/3860013‑3860305
                                                                                                                                                  |                                                                                                                                                  
     ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGct                                                                                                                                            >  2:2377954/1‑150 (MQ=255)
     ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGct                                                                                                                                            <  1:1877819/150‑1 (MQ=255)
        ttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTg                                                                                                                                         <  2:1758962/150‑1 (MQ=255)
              tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTc                                                                                                                                   >  2:4270038/1‑150 (MQ=255)
               ggCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCa                                                                                                                                  <  2:2181156/150‑1 (MQ=255)
               ggCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCa                                                                                                                                  <  2:4860762/150‑1 (MQ=255)
                  tgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTTTACGGAGGGAATCTCTGGGTGTGACCg                                                                                                                               >  1:687555/1‑150 (MQ=255)
                    ctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCAGGCTTTCACCGGc                                                                                                                             >  1:2856813/1‑150 (MQ=255)
                     tCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCAAGTTGTACGGCGGGAACCTCTGGCGTTAACCGGCg                                                                                                                            >  1:4893350/1‑150 (MQ=255)
                     tCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAAGGAGGGAAGCTCAGGCGTTCAcacgcg                                                                                                                            >  1:4368630/1‑145 (MQ=255)
                      ccGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGCTCTGGCTTTAACCGGCGt                                                                                                                           >  2:894672/1‑150 (MQ=255)
                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          >  2:4767666/1‑150 (MQ=255)
                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          <  2:2832955/150‑1 (MQ=255)
                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          <  2:848051/150‑1 (MQ=255)
                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTACGGAGGGAAGCTCTGGGTTTCACCGGCggt                                                                                                                          >  2:1010363/1‑148 (MQ=255)
                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          >  1:3894650/1‑150 (MQ=255)
                        ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTTCGGAGGGAAGCTCTGGCTTTCACCGGCGttt                                                                                                                         >  1:5054660/1‑150 (MQ=255)
                        ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGgtt                                                                                                                         >  1:2519807/1‑150 (MQ=255)
                         gCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtttt                                                                                                                        <  1:4825759/150‑1 (MQ=255)
                         gCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtttt                                                                                                                        <  1:882367/150‑1 (MQ=255)
                          cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGGTTTg                                                                                                                       >  2:1375502/1‑150 (MQ=255)
                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGc                                                                                                                      <  1:142779/150‑1 (MQ=255)
                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTAAGGAGGGAAGCTCTGGCGTTCACCGGCGtttttc                                                                                                                      >  1:3212706/1‑148 (MQ=255)
                             gCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAg                                                                                                                    <  1:2501601/150‑1 (MQ=255)
                             gCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAg                                                                                                                    <  2:3474404/150‑1 (MQ=255)
                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGc                                                                                                                 <  1:967200/150‑1 (MQ=255)
                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGc                                                                                                                 >  2:2168764/1‑150 (MQ=255)
                                  ttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTTATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                               <  2:3103348/150‑1 (MQ=255)
                                  ttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                               <  1:1022466/150‑1 (MQ=255)
                                   tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGtt                                                                                                              <  2:4210809/150‑1 (MQ=255)
                                    aCGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTg                                                                                                             <  2:5170703/150‑1 (MQ=255)
                                     cGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTgg                                                                                                            <  2:5044641/150‑1 (MQ=255)
                                       cTCAGGCGCGAACCAGCCAGATGAATCTCGAAATCTTCCCGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCGGGCTTTCACCGGCGTTTTGCAGTGCGTTGGcc                                                                                                          <  2:3485868/150‑1 (MQ=255)
                                         cAGTCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCAGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGTTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTTGGCCAg                                                                                                        <  2:3170973/150‑1 (MQ=255)
                                         cAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAg                                                                                                        <  2:4561742/150‑1 (MQ=255)
                                         cAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTATATAAAAGGTGTAAGGAAGGAAGCTCTGGCATTAAACCGCGTTTTGCAGTGCGGAGGCCAg                                                                                                        >  1:849981/1‑150 (MQ=255)
                                           ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGtt                                                                                                      <  2:1194186/150‑1 (MQ=255)
                                             cgcgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGGTTg                                                                                                    >  2:4101862/1‑150 (MQ=255)
                                               cgTACCTTCCAGTTGAATCTCTACATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                  <  2:476359/150‑1 (MQ=255)
                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCaa                                                                                                <  1:3227243/150‑1 (MQ=255)
                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTGTTGCAGTGCGGTGGCCCGGTTGTcca                                                                                                >  2:2073936/1‑148 (MQ=255)
                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCCGTGCGTTGGCCAGTTTGTCaa                                                                                                >  2:2073414/1‑150 (MQ=255)
                                                  aCCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCaaa                                                                                               <  1:5172491/150‑1 (MQ=255)
                                                         aGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCGTTTGTCAAAACCGATc                                                                                        >  1:4211863/1‑150 (MQ=255)
                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTTTAAGGGGGGAAGCTCTGGCTTTACACGGCGTTTTTCAGTTCGTTTGGCAGGTTGTGAAAACCCAtac                                                                                       >  2:1570944/1‑148 (MQ=255)
                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCCGTGCGTTGGCCCGGTTGTCAAAACCGAtac                                                                                       >  2:277339/1‑148 (MQ=255)
                                                           ttGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTTTCAAAACCGATCCa                                                                                      >  2:723391/1‑150 (MQ=255)
                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGCGGGGAGCTCAGGCTTTAAACGGCGTTTTTCAGTGCGTTGGCCAGGTTGTCAAAACCGATCCaat                                                                                    >  2:601884/1‑148 (MQ=255)
                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAGTTTGTACGGCGGGAAGCTCAGGCATTCACCCGCGTTTTGCAGTGCGTTGGGCCGGTTTTCAAAACCGATACaat                                                                                    >  1:1085412/1‑148 (MQ=255)
                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGCAGCACTGGCTTTCAACGGCGTTTTGCAGTTCGTTGGCCCGTTTGTCAAAAACGCTCCaat                                                                                    >  1:1641706/1‑148 (MQ=255)
                                                              aaTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTg                                                                                   <  1:2219085/150‑1 (MQ=255)
                                                               aTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGa                                                                                  <  1:2632367/150‑1 (MQ=255)
                                                                    tAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGTGCAGTTTGTCAAAAACGATACAATGACGCAt                                                                             >  1:3759991/1‑150 (MQ=255)
                                                                    tAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGGGGGAAGCTCTGGCTTTCACCGGCGGGTTTGAGTGCGTTGGCCCGTTTTTAAAAAACCATACCATGACGCaa                                                                             >  1:659957/1‑149 (MQ=255)
                                                                        tCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGt                                                                         <  1:4962220/150‑1 (MQ=255)
                                                                        tCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCCTTTGTCAAAACCGATCCATTTACGCATCCGt                                                                         >  1:2988858/1‑150 (MQ=255)
                                                                             cTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGa                                                                    <  2:1573437/150‑1 (MQ=255)
                                                                             cTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGTTTGTCAAAACCGATACATTGACGCATCAGTGGGGa                                                                    >  2:428862/1‑150 (MQ=255)
                                                                              tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAt                                                                   <  1:1163763/150‑1 (MQ=255)
                                                                              tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAt                                                                   <  2:3099082/150‑1 (MQ=255)
                                                                              tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGGTTTGCAGTGCGTTGGCCAGGTTGTCAAAACCGATCCATTGACGCATCAGGGGGGAt                                                                   >  2:2150020/1‑150 (MQ=255)
                                                                                  ggAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAAt                                                               <  2:1521814/150‑1 (MQ=255)
                                                                                   gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                              <  2:1790908/150‑1 (MQ=255)
                                                                                     aaCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTTCGGAGGGAAGCACAGGCGTTCACCGGCGGTTTGCACGGCGGTTGCCCGGTTGTCAAAACCCATACCATGACGCAGCAGAGGGGGTAAATCGa                                                            >  2:4948457/1‑150 (MQ=255)
                                                                                         tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGtt                                                        <  1:1731601/150‑1 (MQ=255)
                                                                                            cAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACg                                                     <  1:3642431/150‑1 (MQ=255)
                                                                                                gcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATa                                                 <  1:2896742/150‑1 (MQ=255)
                                                                                                 cAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAg                                                <  2:941279/150‑1 (MQ=255)
                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:4408842/150‑1 (MQ=255)
                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:4467045/150‑1 (MQ=255)
                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:2099206/150‑1 (MQ=255)
                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:475424/150‑1 (MQ=255)
                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:4562552/150‑1 (MQ=255)
                                                                                                     ggTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTACGGTGGGCAGCTATGGCGTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTTTCAAAACCCATCCATTTTAGCATCAGTGGGGATAAAAAGAAGTTACGCAAAGTcct                                            >  1:5115577/1‑148 (MQ=255)
                                                                                                     ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAACGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATATTCAt                                            >  2:3682894/1‑150 (MQ=255)
                                                                                                               tAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCt                                  <  2:1933183/150‑1 (MQ=255)
                                                                                                                aGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTa                                 <  1:1174569/150‑1 (MQ=255)
                                                                                                                      tATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCCAAACCGATCCATTGACGCATCACTGGGGATAAATCGAAGTTACGCATAATAATTTCTCCATCTAAGAAGc                           >  2:3832592/1‑150 (MQ=255)
                                                                                                                       aTCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCg                          <  1:649176/150‑1 (MQ=255)
                                                                                                                         cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTTCGTTGGCCAGTTTTTAAAAACCGATCCAATGACGCAACAGTGGGGATAAATAGAAGTTAACCAAATTCAATTATCCATCAAAGAAGCGag                        >  2:39993/1‑150 (MQ=255)
                                                                                                                         cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGag                        <  2:1803278/150‑1 (MQ=255)
                                                                                                                         cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAATGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCATCTAAGAAGCGag                        >  2:2585054/1‑150 (MQ=255)
                                                                                                                           tcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagta                      <  2:324674/150‑1 (MQ=255)
                                                                                                                            cgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaa                     <  2:1221859/150‑1 (MQ=255)
                                                                                                                             gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTAATTTATCCTTCTAAGAAGCGagtaag                    >  1:4915916/1‑150 (MQ=255)
                                                                                                                              cTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagt                   >  1:241192/1‑150 (MQ=255)
                                                                                                                               ttttCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagta                  <  1:3300116/150‑1 (MQ=255)
                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt               <  2:2519807/150‑1 (MQ=255)
                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt               <  2:1335055/150‑1 (MQ=255)
                                                                                                                                   ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg              <  1:2701396/150‑1 (MQ=255)
                                                                                                                                    tcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGc             <  1:2014652/150‑1 (MQ=255)
                                                                                                                                     cAATGTTGTCCGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTGGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCa            <  2:3813753/150‑1 (MQ=255)
                                                                                                                                      aaTGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCaa           <  1:4085908/150‑1 (MQ=255)
                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc       <  1:3064147/150‑1 (MQ=255)
                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc       <  1:3546211/150‑1 (MQ=255)
                                                                                                                                           tgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCg      >  1:862970/1‑150 (MQ=255)
                                                                                                                                           tgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCg      <  1:2222187/150‑1 (MQ=255)
                                                                                                                                            gtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGa     <  2:4469808/150‑1 (MQ=255)
                                                                                                                                             tACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGaa    >  1:3457156/1‑150 (MQ=255)
                                                                                                                                               cggaggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGa  >  2:3525947/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGA  >  CP009273/3860013‑3860305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860011‑3860308
                                                                                                                                                    |                                                                                                                                                     
ATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAG                                                                                                                                                      <  SRR19973083.5108614/150‑1 (MQ=60)
  TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGG                                                                                                                                                    <  SRR19973083.4530819/150‑1 (MQ=60)
   gtgtgctcttccgatctTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGA                                                                                                                                                   <  SRR19973083.1626582/133‑1 (MQ=60)
     TTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTGTACGGAGGGAAG                                                                                                                                                 >  SRR19973083.3303233/1‑150 (MQ=60)
       CTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCT                                                                                                                                               <  SRR19973083.1887333/150‑1 (MQ=60)
          TTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTG                                                                                                                                            >  SRR19973083.3478671/1‑150 (MQ=60)
          TTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTG                                                                                                                                            <  SRR19973083.1816928/150‑1 (MQ=60)
            CTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTAAGGAGGGGAGCTCTGGC                                                                                                                                          >  SRR19973083.1657599/1‑150 (MQ=60)
            CTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAATGGTTATCGTCGCTTTTATCAATGGTGTTAGGAGGGTAGCTCTGGC                                                                                                                                          >  SRR19973083.4488636/1‑150 (MQ=60)
                 GGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCA                                                                                                                                     <  SRR19973083.5186086/150‑1 (MQ=60)
                    TGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTGAATGTTGTTCGGAGGGGAGCTCTGGCGTTCACCG                                                                                                                                  >  SRR19973083.690767/1‑150 (MQ=60)
                    TGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTTTACGGAGGGAATCTCTGGgtgtgaccg                                                                                                                                  >  SRR19973083.690991/1‑141 (MQ=60)
                      CTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCAGGCTTTCACCGGC                                                                                                                                >  SRR19973083.2870902/1‑150 (MQ=60)
                      CTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTAAGGAGGGAAGCTCTGGCTTTAACCCGC                                                                                                                                >  SRR19973083.2715681/1‑150 (MQ=60)
                       TCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCAAGTTGTACGGCGGGAACCTCTGGCGTTAACCGGCG                                                                                                                               >  SRR19973083.4914382/1‑150 (MQ=60)
                       TCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATGAATGTTTTTAGGAGGGAAGCTCTGGCTTTAACCGGCG                                                                                                                               >  SRR19973083.4914358/1‑150 (MQ=60)
                       TCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTTTTAGGAGGGGAGCTCTGGCTTTAAACGGCG                                                                                                                               >  SRR19973083.4914455/1‑150 (MQ=60)
                       TCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAAGGAGGGAAGCTCaggcgttcacacgcg                                                                                                                               >  SRR19973083.4387603/1‑135 (MQ=60)
                         CGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTT                                                                                                                             >  SRR19973083.3912006/1‑150 (MQ=60)
                          GGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTTCGGAGGGAAGCTCTGGCTTTCACCGGCGTTT                                                                                                                            >  SRR19973083.5076306/1‑150 (MQ=60)
                          GGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTT                                                                                                                            >  SRR19973083.5076482/1‑150 (MQ=60)
                          GGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTT                                                                                                                            >  SRR19973083.2532614/1‑150 (MQ=60)
                          GGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGAAGCTCTGGCTTTCACCGGCGTTT                                                                                                                            >  SRR19973083.2937126/1‑150 (MQ=60)
                          GGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTTTAAGGAGGGAAGCTCTGGGTTTAACCGGCGGTT                                                                                                                            >  SRR19973083.2532765/1‑150 (MQ=60)
                           GCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTT                                                                                                                           <  SRR19973083.4846458/150‑1 (MQ=60)
                           GCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTT                                                                                                                           <  SRR19973083.886522/150‑1 (MQ=60)
                             GTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGC                                                                                                                         <  SRR19973083.143652/150‑1 (MQ=60)
                             GTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGC                                                                                                                         >  SRR19973083.3228141/1‑150 (MQ=60)
                             GTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCGGGCTTTCACCGGCGGTTTGC                                                                                                                         >  SRR19973083.3228136/1‑150 (MQ=60)
                             GTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTAAGGAGGGAAGCTCTGGCGTTCACCGGCGTTTTTC                                                                                                                         >  SRR19973083.3227943/1‑150 (MQ=60)
                               GCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTCAATCTTCCTGACGGTAACCTGCCAGCGCAAGGGTAATGCGGTATTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAG                                                                                                                       <  SRR19973083.2514454/150‑1 (MQ=60)
                               GCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAG                                                                                                                       <  SRR19973083.2514350/150‑1 (MQ=60)
                               GCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAG                                                                                                                       <  SRR19973083.2514184/150‑1 (MQ=60)
                               GCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAG                                                                                                                       <  SRR19973083.2223741/150‑1 (MQ=60)
                               GCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAAAGTTGTAAGGAGGGAAGCTCTTGCTTTAAACGGCGGTTTGAAG                                                                                                                       >  SRR19973083.66363/1‑150 (MQ=60)
                                  TTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC                                                                                                                    <  SRR19973083.3050922/150‑1 (MQ=60)
                                  TTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC                                                                                                                    <  SRR19973083.971978/150‑1 (MQ=60)
                                  TTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGC                                                                                                                    <  SRR19973083.10680/150‑1 (MQ=60)
                                    TTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGT                                                                                                                  <  SRR19973083.1027587/150‑1 (MQ=60)
                                               CGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTG                                                                                                       <  SRR19973083.894810/150‑1 (MQ=60)
                                                   TACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAA                                                                                                   <  SRR19973083.3242527/150‑1 (MQ=60)
                                                    ACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAA                                                                                                  <  SRR19973083.5194419/150‑1 (MQ=60)
                                                      CTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTACGGAGGGAAGCTATTGCTTTCAACGGCGGTTTTCAGTTCGTTGGCCACTTTTTCCAAAC                                                                                                >  SRR19973083.827656/1‑150 (MQ=60)
                                                           AGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCGTTTGTCAAAACCGATC                                                                                           >  SRR19973083.4230357/1‑150 (MQ=60)
                                                           AGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTACGGTGGGAAGCTCTGGGGTTCACCGGCGGTTTGCCTTGCGGTGGCCCGTTTGTCAAAAACGAAC                                                                                           >  SRR19973083.2908766/1‑150 (MQ=60)
                                                              TGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAT                                                                                        >  SRR19973083.3848589/1‑150 (MQ=60)
                                                               GAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAGTTTGTACGGCGGGAAGCTCAGGCATTCACCCGCGTTTTGCAGTGCGTTGGgccggttttcaaaaccgatacaat                                                                                       >  SRR19973083.1090874/1‑126 (MQ=60)
                                                               GAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGCAGCACTGGCTTTCAACGGCGTTTTGCAGTTCGTTGGCCCGTTTGTCAAAAacgctccaat                                                                                       >  SRR19973083.1649881/1‑140 (MQ=60)
                                                                AATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTG                                                                                      <  SRR19973083.2230451/150‑1 (MQ=60)
                                                                AATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTG                                                                                      <  SRR19973083.2230298/150‑1 (MQ=60)
                                                                 ATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGA                                                                                     <  SRR19973083.2645588/150‑1 (MQ=60)
                                                                    TCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCAGGTTTTCAAAACCGATACATTGACGC                                                                                  >  SRR19973083.424802/1‑150 (MQ=60)
                                                                      TAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGTGCAGTTTGTCAAAAACGATACAATGACGCAT                                                                                >  SRR19973083.3777015/1‑150 (MQ=60)
                                                                      TAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATGAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAACGATACATTTAAGCAT                                                                                >  SRR19973083.3776948/1‑150 (MQ=60)
                                                                      TAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGGGGGAAGCTCTGGCTTTCACCGGCGggtttgagtgcgttggcccgtttttaaaaaaccataccatgacgcaa                                                                                >  SRR19973083.663259/1‑103 (MQ=60)
                                                                       AAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTTTACGGAGGGTAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGGTTGCCAGTTTTTCAAAACCCATACAATGACGCAGC                                                                               >  SRR19973083.2183778/1‑150 (MQ=60)
                                                                          TCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGT                                                                            <  SRR19973083.4983537/150‑1 (MQ=60)
                                                                          TCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCCTTTGTCAAAACCGATCCATTTACGCATCCGT                                                                            >  SRR19973083.3003336/1‑150 (MQ=60)
                                                                            TTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGG                                                                          <  SRR19973083.1238394/150‑1 (MQ=60)
                                                                            TTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAAAGTTGTACGGAGGGAAGCTCTGGCGTTCACCGGCGGTTTGGAGTGCGGTGGCCAGTTTGTCAAAAaccctcaagttacgcatcagtgg                                                                          >  SRR19973083.2031577/1‑127 (MQ=60)
                                                                                TGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAT                                                                      <  SRR19973083.1169885/150‑1 (MQ=60)
                                                                                TGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAT                                                                      <  SRR19973083.1169552/150‑1 (MQ=60)
                                                                                   CGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAA                                                                   <  SRR19973083.4879966/150‑1 (MQ=60)
                                                                                   CGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAA                                                                   <  SRR19973083.2476276/150‑1 (MQ=60)
                                                                                    GGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTGTCAAAGTTTTACGGGGGGAAGCACGGGGTTTCACCGGCGGTTTGGCGTGCGTTGGCCcggttttaaaaaaccatcccttttcgcatcactggggataaat                                                                  >  SRR19973083.3143559/1‑107 (MQ=53)
                                                                                          CTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGT                                                            <  SRR19973083.411101/150‑1 (MQ=60)
                                                                                           TGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTT                                                           <  SRR19973083.1740090/150‑1 (MQ=60)
                                                                                             CCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTAC                                                         <  SRR19973083.1136747/150‑1 (MQ=60)
                                                                                              CAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACG                                                        <  SRR19973083.3659093/150‑1 (MQ=60)
                                                                                               AGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTTTACGGAGGGAAGCTCAGGGGTTCACCGGCGTTTTGCAGTGCGGTGGCCCGTTTGTCAAAACCGATACATTTTCGCCTCAGGTGGGATAAATAGAAGTTACGC                                                       >  SRR19973083.3415499/1‑150 (MQ=60)
                                                                                                GCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCA                                                      <  SRR19973083.56972/150‑1 (MQ=60)
                                                                                                  GCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATA                                                    <  SRR19973083.2910941/150‑1 (MQ=60)
                                                                                                     AGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTTTAAGGAGGGAAGCTCTGGGTTTCACCcgcggtttggcgttggttgggcagtttgtaaaaaaacatcaaattaagcaaaaatgggggtaaaaagaagtttcgcatagta                                                 >  SRR19973083.4562580/1‑68 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.4427994/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.4428029/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.4485970/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.4486450/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.4582300/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.477629/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.477676/150‑1 (MQ=60)
                                                                                                      GGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCA                                                <  SRR19973083.2109843/150‑1 (MQ=60)
                                                                                                       GGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTACGGTGGGCAGCTATGGCGTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTTTCAAAACCCATCCATTTTAGCATCAGTGGGGATAAAAAGAAGTTACGCAAAGTCCT                                               >  SRR19973083.5137374/1‑150 (MQ=60)
                                                                                                       GGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGTGGGAAGCTCTGGGTTTCACCGGCGTTTTGCAGTTCGTTGGCCAGTTTGTAAAAAACGATCCAATTACGCATAAGTGGGGTTAAATAGTAGTTACGCATAttaat                                               >  SRR19973083.5137372/1‑145 (MQ=60)
                                                                                                        GTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATT                                              <  SRR19973083.265033/150‑1 (MQ=60)
                                                                                                           ATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCT                                           <  SRR19973083.869136/150‑1 (MQ=60)
                                                                                                             GCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCC                                         <  SRR19973083.483412/150‑1 (MQ=60)
                                                                                                                 TAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCT                                     <  SRR19973083.96935/150‑1 (MQ=60)
                                                                                                                  AGTGGTTATCGTCGCTTTTCTCAATGTTGTCCGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTA                                    <  SRR19973083.1180526/150‑1 (MQ=60)
                                                                                                                  AGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTA                                    <  SRR19973083.1180403/150‑1 (MQ=60)
                                                                                                                   GTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAA                                   <  SRR19973083.721594/150‑1 (MQ=60)
                                                                                                                         ATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCG                             <  SRR19973083.652440/150‑1 (MQ=60)
                                                                                                                          TCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGA                            <  SRR19973083.3537726/150‑1 (MQ=60)
                                                                                                                           CGTCGCTTTTCTCAATGTTGTTCGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAG                           <  SRR19973083.1578835/150‑1 (MQ=60)
                                                                                                                             TCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTA                         <  SRR19973083.3569904/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAA                        <  SRR19973083.975234/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGTCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTTACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAA                        <  SRR19973083.4975612/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAA                        <  SRR19973083.4363457/150‑1 (MQ=60)
                                                                                                                               GCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTAATTTATCCTTCTAAGAAGCGAGTAAG                       >  SRR19973083.4937041/1‑150 (MQ=60)
                                                                                                                               GCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGGGGGGATAAATAGAATTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAG                       >  SRR19973083.2345271/1‑150 (MQ=60)
                                                                                                                                CTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGT                      >  SRR19973083.5014956/1‑150 (MQ=60)
                                                                                                                                CTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGT                      >  SRR19973083.570506/1‑150 (MQ=60)
                                                                                                                                CTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGT                      >  SRR19973083.242490/1‑150 (MQ=60)
                                                                                                                                 TTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTA                     <  SRR19973083.3315625/150‑1 (MQ=60)
                                                                                                                                     CTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTG                 <  SRR19973083.2714921/150‑1 (MQ=60)
                                                                                                                                      TCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGC                <  SRR19973083.2024944/150‑1 (MQ=60)
                                                                                                                                        AATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAA              <  SRR19973083.4104016/150‑1 (MQ=60)
                                                                                                                                          TGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAAT            <  SRR19973083.2918218/150‑1 (MQ=60)
                                                                                                                                            TTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCC          <  SRR19973083.186841/150‑1 (MQ=60)
                                                                                                                                            TTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCC          <  SRR19973083.3078834/150‑1 (MQ=60)
                                                                                                                                            TTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCC          <  SRR19973083.3562490/150‑1 (MQ=60)
                                                                                                                                            TTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCC          <  SRR19973083.3902652/150‑1 (MQ=60)
                                                                                                                                            TTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCC          <  SRR19973083.4092547/150‑1 (MQ=60)
                                                                                                                                             TGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCG         >  SRR19973083.865823/1‑150 (MQ=60)
                                                                                                                                             TGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCG         >  SRR19973083.867009/1‑150 (MQ=60)
                                                                                                                                             TGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCG         <  SRR19973083.2233422/150‑1 (MQ=60)
                                                                                                                                               TACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAA       >  SRR19973083.3473001/1‑150 (MQ=60)
                                                                                                                                                    AGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  SRR19973083.2821779/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860011‑3860308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑