Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,604,068 G→T 9.7% T290T (ACC→ACA)  uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,604,0680GT 9.7% 667.0 / 42.2 196T290T (ACC→ACA) uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (91/86);  new base T (8/11);  total (99/97)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 205 total aligned reads displayed.

CGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAG  >  CP009273/1603920‑1604216
                                                                                                                                                    |                                                                                                                                                    
 gAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGgt                                                                                                                                                    <  1:4834848/150‑1 (MQ=255)
 gAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGgt                                                                                                                                                    >  2:1029360/1‑150 (MQ=255)
 gAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGgt                                                                                                                                                    >  2:1029652/1‑150 (MQ=255)
  aaaGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGgtg                                                                                                                                                   >  2:1858805/1‑150 (MQ=255)
     gATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTgc                                                                                                                                                >  2:3000047/1‑150 (MQ=255)
       ttCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTgcgc                                                                                                                                              >  2:4196183/1‑150 (MQ=255)
       ttCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTgcgc                                                                                                                                              >  2:4194712/1‑150 (MQ=255)
       ttCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTgcgc                                                                                                                                              >  2:1604992/1‑150 (MQ=255)
        tCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCa                                                                                                                                             <  2:1737272/150‑1 (MQ=255)
          cAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCAcc                                                                                                                                           <  2:1624761/150‑1 (MQ=255)
          cAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCAcc                                                                                                                                           >  1:2756760/1‑150 (MQ=255)
             ttCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGtt                                                                                                                                        <  1:3006492/150‑1 (MQ=255)
              tcatcaCGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTg                                                                                                                                       >  2:238483/1‑150 (MQ=255)
              tcatcaCGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTg                                                                                                                                       >  1:2580033/1‑150 (MQ=255)
                atcaCGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTATCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAg                                                                                                                                     <  2:5129715/150‑1 (MQ=255)
                    cGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGAtt                                                                                                                                 >  1:2523041/1‑150 (MQ=255)
                    cGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGAtt                                                                                                                                 <  1:2456228/150‑1 (MQ=255)
                       ggCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGcc                                                                                                                              >  1:937266/1‑150 (MQ=255)
                       ggCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGcc                                                                                                                              <  2:1918868/150‑1 (MQ=255)
                       ggCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGcc                                                                                                                              <  2:2869998/150‑1 (MQ=255)
                       ggCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCGACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGcc                                                                                                                              <  2:2377747/150‑1 (MQ=255)
                         cAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCAc                                                                                                                            >  2:680782/1‑150 (MQ=255)
                         cAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCGTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCAc                                                                                                                            <  2:5096269/150‑1 (MQ=255)
                          aaaTCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACt                                                                                                                           >  1:4922764/1‑150 (MQ=255)
                          aaaTCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACt                                                                                                                           >  1:1524914/1‑150 (MQ=255)
                          aaaTCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACt                                                                                                                           >  1:2333920/1‑150 (MQ=255)
                            aTCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACttt                                                                                                                         <  2:4968101/150‑1 (MQ=255)
                            aTCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGGTGAGGATTGCCACttt                                                                                                                         >  1:44566/1‑150 (MQ=255)
                               cAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCg                                                                                                                      <  2:3462726/150‑1 (MQ=255)
                                aGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGt                                                                                                                     <  1:5089262/150‑1 (MQ=255)
                                 gTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGtt                                                                                                                    >  2:4026383/1‑150 (MQ=255)
                                  tACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTc                                                                                                                   <  1:2907902/150‑1 (MQ=255)
                                   aCCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCt                                                                                                                  <  1:3185284/150‑1 (MQ=255)
                                   aCCGGAATAATTTCTTCGTAGATAGCTTTGTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCt                                                                                                                  <  2:3773905/150‑1 (MQ=255)
                                     cGGAATAATTTCTTCGTAGATAACTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCttt                                                                                                                >  2:1546871/1‑150 (MQ=255)
                                      ggAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTg                                                                                                               <  1:3149335/150‑1 (MQ=255)
                                        aataatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTa                                                                                                             <  1:2421982/150‑1 (MQ=255)
                                        aataatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTa                                                                                                             >  1:1419739/1‑150 (MQ=255)
                                        aataatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTa                                                                                                             <  1:5002433/150‑1 (MQ=255)
                                        aataatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTa                                                                                                             >  1:4549848/1‑150 (MQ=255)
                                          taatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACg                                                                                                           <  2:2911148/150‑1 (MQ=255)
                                          taatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACg                                                                                                           >  1:1020283/1‑150 (MQ=255)
                                           aatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACgg                                                                                                          >  1:501054/1‑150 (MQ=255)
                                           aatTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACgg                                                                                                          >  1:4436248/1‑150 (MQ=255)
                                              ttcttcGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGttt                                                                                                       >  1:2960181/1‑150 (MQ=255)
                                              ttcttcGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGttt                                                                                                       <  2:2424525/150‑1 (MQ=255)
                                              ttcttcGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGttt                                                                                                       >  1:348825/1‑150 (MQ=255)
                                                     tAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCg                                                                                                >  2:389671/1‑150 (MQ=255)
                                                     tAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCg                                                                                                >  1:869232/1‑150 (MQ=255)
                                                      aGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGt                                                                                               >  1:3010849/1‑150 (MQ=255)
                                                      aGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGt                                                                                               >  1:3532125/1‑150 (MQ=255)
                                                       gATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTc                                                                                              <  1:3344548/150‑1 (MQ=255)
                                                               tttCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCa                                                                                      <  1:4978906/150‑1 (MQ=255)
                                                                ttCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAg                                                                                     <  2:5060549/150‑1 (MQ=255)
                                                                 tCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGc                                                                                    <  2:3401960/150‑1 (MQ=255)
                                                                   aaCGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCAc                                                                                  >  1:3136330/1‑150 (MQ=255)
                                                                     cGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGt                                                                                >  1:4905456/1‑150 (MQ=255)
                                                                      gAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTTCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGtt                                                                               <  2:4809487/150‑1 (MQ=255)
                                                                      gAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTGTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGtt                                                                               >  2:4576314/1‑150 (MQ=255)
                                                                       anTGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTg                                                                              >  1:3759154/1‑150 (MQ=255)
                                                                        aTGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGa                                                                             <  1:4022484/150‑1 (MQ=255)
                                                                            gcAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCgg                                                                         <  2:4430299/150‑1 (MQ=255)
                                                                             cAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGa                                                                        <  1:3862131/150‑1 (MQ=255)
                                                                              aaaTTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGat                                                                       <  1:3223120/150‑1 (MQ=255)
                                                                              aaaTTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGat                                                                       >  1:2069552/1‑150 (MQ=255)
                                                                                  ttCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATAttt                                                                   >  1:135287/1‑150 (MQ=255)
                                                                                   tCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTg                                                                  <  2:3067206/150‑1 (MQ=255)
                                                                                    cAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGt                                                                 >  1:641884/1‑150 (MQ=255)
                                                                                    cAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATACTTGt                                                                 >  1:3361971/1‑150 (MQ=255)
                                                                                          cGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGAc                                                           <  2:4127788/150‑1 (MQ=255)
                                                                                           gTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACg                                                          <  2:2205326/150‑1 (MQ=255)
                                                                                             tCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCa                                                        <  2:1289374/150‑1 (MQ=255)
                                                                                                   cTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAg                                                  >  2:1708966/1‑150 (MQ=255)
                                                                                                    ttCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGt                                                 <  2:1027911/150‑1 (MQ=255)
                                                                                                     tCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTc                                                <  1:399046/150‑1 (MQ=255)
                                                                                                       aCCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGc                                              >  2:31852/1‑150 (MQ=255)
                                                                                                          tACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTaa                                           <  2:2772447/150‑1 (MQ=255)
                                                                                                            cGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAgg                                         >  1:5158539/1‑150 (MQ=255)
                                                                                                                aTCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGAttt                                     <  1:4041153/150‑1 (MQ=255)
                                                                                                                 tCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCTACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTc                                    <  1:4330675/150‑1 (MQ=255)
                                                                                                                 tCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTc                                    <  1:4322608/150‑1 (MQ=255)
                                                                                                                  ccAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCg                                   >  1:1885407/1‑150 (MQ=255)
                                                                                                                   cAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCgg                                  >  2:1979912/1‑150 (MQ=255)
                                                                                                                    aGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGt                                 <  1:4923283/150‑1 (MQ=255)
                                                                                                                     gCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTc                                >  2:2544340/1‑150 (MQ=255)
                                                                                                                         tgcctgGAACGCCACCGGCACCAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTg                            <  1:3188073/150‑1 (MQ=255)
                                                                                                                             tgGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATc                        >  2:57618/1‑150 (MQ=255)
                                                                                                                              ggAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATca                       <  1:4599861/150‑1 (MQ=255)
                                                                                                                              ggAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATca                       >  1:4156214/1‑150 (MQ=255)
                                                                                                                                  cGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACaaa                   >  1:370888/1‑150 (MQ=255)
                                                                                                                                        cGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTa             <  1:3593516/150‑1 (MQ=255)
                                                                                                                                            aCCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAg         >  2:3373708/1‑150 (MQ=255)
                                                                                                                                             ccAGCGCTGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGACCCTGAATCACAAACAGGTAAAAgt        <  1:3052221/150‑1 (MQ=255)
                                                                                                                                                gcgcGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGtt     <  1:1209712/150‑1 (MQ=255)
                                                                                                                                                gcgcGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGtt     <  2:278633/150‑1 (MQ=255)
                                                                                                                                                 cgctGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTc    >  1:685813/1‑150 (MQ=255)
                                                                                                                                                 cgcgGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTc    <  2:3685370/150‑1 (MQ=255)
                                                                                                                                                 cgcgGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTc    <  1:680782/150‑1 (MQ=255)
                                                                                                                                                   cgGTGTGCGCACCGTTGAGGATTGTCACTGTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGTATCACAAACAGGTAAAAGTGTTCAg  <  1:927276/150‑1 (MQ=255)
                                                                                                                                                   cgGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAg  <  2:708532/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTTCTTCGTAGATAGCTTTTTCAACGAATGCGCAAATTTCAGCATCGTTCATCGCTTCACCTACGGTATCCAGCCCTGCCTGGAACGCCACCGGCACCAGCGCGGTGTGCGCACCGTTGAGGATTGCCACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAG  >  CP009273/1603920‑1604216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A